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The map label for this gene is btuE [H]

Identifier: 119714404

GI number: 119714404

Start: 151665

End: 152153

Strand: Reverse

Name: btuE [H]

Synonym: Noca_0137

Alternate gene names: 119714404

Gene position: 152153-151665 (Counterclockwise)

Preceding gene: 119714407

Following gene: 119714403

Centisome position: 3.05

GC content: 71.17

Gene sequence:

>489_bases
ATGAGCATCCTCGACGCCAAGATCGCCCGCCTGGACGGGACCCCGAGCACGCTGGGCGAGATCACCGGCGGCCGGCCCGC
GCTGTTGGTCAACGTCGCCAGCAAGTGCGGGCTGACCCCGCAGTACGCCGGGCTCGAGGAGCTCCACGAGCGGCTCGCGG
ACCGCGGCTTCACGGTGGTCGGGCTGCCGTGCAACCAGTTCCGCGGGCAGGAGCCGGGGACCGCGGACGAGATCGCCGAG
TTCTGCTCCGCGACGTACGGCGTCACCTTCCCGATGACCGAGAAGATCGACGTCAACGGCCCGGACCGCCACGAGATCTA
CCGGACCCTCGTCGACACCCCGAACGAGTCCGGCGAGAGCGGCGACATCACGTGGAACTTCGAGAAGTTCCTCGTCGACG
CCTCCGGCGCGGTCCTCGCCCGGTTCAGCCCCGGCGTCGAGCCCGGCGACCCCCGCCTGGTCGCCGCCGTCGAGTCCCAG
GTCGGCTGA

Upstream 100 bases:

>100_bases
CACGGAACGTCGCCGGGCCGGTGACCGGGACGAAGCTCACGCCCCGACGCTAGCCGGACGGTCCGACGGTTCCCTCCCGG
CCGCGACGTAGCCTGGCCGC

Downstream 100 bases:

>100_bases
CTCCCCTGTCCTCACTCGGTGACCCAGCCCACAGGCCGGAGAGCCGAAAGTAGGGTGCTGAGCCCAGGTAGGCTCACCTA
GCCTCTTCCGGTGACCGAGA

Product: glutathione peroxidase

Products: glutathione disulfide; H2O

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MSILDAKIARLDGTPSTLGEITGGRPALLVNVASKCGLTPQYAGLEELHERLADRGFTVVGLPCNQFRGQEPGTADEIAE
FCSATYGVTFPMTEKIDVNGPDRHEIYRTLVDTPNESGESGDITWNFEKFLVDASGAVLARFSPGVEPGDPRLVAAVESQ
VG

Sequences:

>Translated_162_residues
MSILDAKIARLDGTPSTLGEITGGRPALLVNVASKCGLTPQYAGLEELHERLADRGFTVVGLPCNQFRGQEPGTADEIAE
FCSATYGVTFPMTEKIDVNGPDRHEIYRTLVDTPNESGESGDITWNFEKFLVDASGAVLARFSPGVEPGDPRLVAAVESQ
VG
>Mature_161_residues
SILDAKIARLDGTPSTLGEITGGRPALLVNVASKCGLTPQYAGLEELHERLADRGFTVVGLPCNQFRGQEPGTADEIAEF
CSATYGVTFPMTEKIDVNGPDRHEIYRTLVDTPNESGESGDITWNFEKFLVDASGAVLARFSPGVEPGDPRLVAAVESQV
G

Specific function: Not essential for B12 transport; however, it is an auxiliary component of the transport system [H]

COG id: COG0386

COG function: function code O; Glutathione peroxidase

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutathione peroxidase family [H]

Homologues:

Organism=Homo sapiens, GI75709200, Length=140, Percent_Identity=42.1428571428571, Blast_Score=105, Evalue=2e-23,
Organism=Homo sapiens, GI90903240, Length=140, Percent_Identity=42.1428571428571, Blast_Score=104, Evalue=3e-23,
Organism=Homo sapiens, GI15618997, Length=162, Percent_Identity=38.8888888888889, Blast_Score=100, Evalue=7e-22,
Organism=Homo sapiens, GI192455698, Length=126, Percent_Identity=39.6825396825397, Blast_Score=96, Evalue=1e-20,
Organism=Homo sapiens, GI90903238, Length=106, Percent_Identity=44.3396226415094, Blast_Score=87, Evalue=8e-18,
Organism=Homo sapiens, GI4557629, Length=139, Percent_Identity=38.8489208633094, Blast_Score=84, Evalue=5e-17,
Organism=Homo sapiens, GI6006001, Length=160, Percent_Identity=34.375, Blast_Score=80, Evalue=5e-16,
Organism=Homo sapiens, GI33186887, Length=155, Percent_Identity=35.4838709677419, Blast_Score=78, Evalue=4e-15,
Organism=Homo sapiens, GI41406084, Length=155, Percent_Identity=33.5483870967742, Blast_Score=74, Evalue=5e-14,
Organism=Escherichia coli, GI1788003, Length=176, Percent_Identity=46.5909090909091, Blast_Score=155, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI71993584, Length=150, Percent_Identity=38, Blast_Score=102, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17506887, Length=152, Percent_Identity=36.1842105263158, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17535473, Length=142, Percent_Identity=39.4366197183099, Blast_Score=96, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17550320, Length=170, Percent_Identity=35.8823529411765, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI193209493, Length=164, Percent_Identity=34.1463414634146, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI193210707, Length=151, Percent_Identity=33.7748344370861, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319721, Length=127, Percent_Identity=52.755905511811, Blast_Score=147, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6322228, Length=146, Percent_Identity=50.6849315068493, Blast_Score=146, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6322826, Length=139, Percent_Identity=43.1654676258993, Blast_Score=117, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24656767, Length=156, Percent_Identity=37.1794871794872, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24656777, Length=156, Percent_Identity=37.1794871794872, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24656772, Length=138, Percent_Identity=39.1304347826087, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI85726443, Length=138, Percent_Identity=37.6811594202899, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000889
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00255 GSHPx [H]

EC number: 1.11.1.9

Molecular weight: Translated: 17283; Mature: 17151

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILDAKIARLDGTPSTLGEITGGRPALLVNVASKCGLTPQYAGLEELHERLADRGFTVV
CCCCCCHHHHCCCCCHHHHHCCCCCCEEEEEEHHHCCCCCCCCCHHHHHHHHHHCCCEEE
GLPCNQFRGQEPGTADEIAEFCSATYGVTFPMTEKIDVNGPDRHEIYRTLVDTPNESGES
ECCHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHCCCCCCCCC
GDITWNFEKFLVDASGAVLARFSPGVEPGDPRLVAAVESQVG
CCEEEEHHHEEEECCCCEEEEECCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure 
SILDAKIARLDGTPSTLGEITGGRPALLVNVASKCGLTPQYAGLEELHERLADRGFTVV
CCCCCHHHHCCCCCHHHHHCCCCCCEEEEEEHHHCCCCCCCCCHHHHHHHHHHCCCEEE
GLPCNQFRGQEPGTADEIAEFCSATYGVTFPMTEKIDVNGPDRHEIYRTLVDTPNESGES
ECCHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHCCCCCCCCC
GDITWNFEKFLVDASGAVLARFSPGVEPGDPRLVAAVESQVG
CCEEEEHHHEEEECCCCEEEEECCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glutathione; H2O2

Specific reaction: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3528129; 9097039; 9278503 [H]