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The map label for this gene is emrB [H]

Identifier: 119714403

GI number: 119714403

Start: 150060

End: 151574

Strand: Reverse

Name: emrB [H]

Synonym: Noca_0136

Alternate gene names: 119714403

Gene position: 151574-150060 (Counterclockwise)

Preceding gene: 119714404

Following gene: 119714399

Centisome position: 3.04

GC content: 70.76

Gene sequence:

>1515_bases
GTGACCGAGACCCAGGACGAGCGCACCGCCCCGGCCCCAGGCCGCCCCGTCGACGCCCCCGACGCACCTCCGGAGGGACT
CGACCGCCGGGTGCTGATGGTCGCCGGGGTGGTCGTGCTCGGCGCGATCATGTCGATCCTCGACATCACGGTGGTCTCGG
TCGCACTGCAGACCTTCCAGCAGGAGTTCGACGCGACCTCGGCCGAGGTCGCGTGGACGATGACCGGCTACACCCTCGCC
CTGGCCAGCGTGATCCCGCTGACCGGCTGGGCGGCCGACCGGTTCGGCACCAAGCGCCTCTACCTGCTCGCCGTCTTGCT
GTTCACCGCCGGATCCGTGCTGTGCGCGGCGGCCACGTCGCTCGAGATGCTGGTGAGCTTCCGGGTCCTGCAGGGCGTCG
GCGGCGGCATGCTGATGCCGCTGGGCATGACGATCCTGACTCGGGCCGCCGGCCCGGAGCGGGTCGGCCGGGTGATGGCG
GTGCTCGGCATCCCGATGCTGCTCGGCCCGATCTTCGGCCCGATCCTCGGCGGCGCCCTGATCGACTCCGCGAGCTGGCA
CTGGATCTTCCTGATCAACCTGCCCATCGGGATCGCCGCCCTCGCCTACGCCGCCGTCGTGCTGCCGCGCGACCACGTCG
AGCCGTCCGAGACCTTCGACTGGCTCGGCATGGTCCTGCTCTCCCCCGGGCTCGCCGCGTTCCTGTACGGCGTCTCCTCG
ATCCCGGCAGCACGTGAGGAGCACGGCACCCTCTGGACGACCGAGGTGGTCGCGCCCGCGCTCGCCGGCCTGCTGCTGAT
CGCGGCCTTCGTGCCGTGGGCCCTCAACCGCCGCAACCTCCACCCGCTGGTCGACCTGCGGCTGTTCGGCAACGGCACGA
TGACGGCCGCGGTGCTGTCGATGTCGCTGTTCGCGATCGCGTTCTTCGGCGCCAGCCTGCTGTTCCCGCTGTACTTCCAG
TGGGTGCGCGGCGAGGACGCGCTCGGCGCCGGCCTGCTGCTGGCACCGCAGGGCGTCGGCGCGATGATCACGATGCCGAT
CGCGGGTGTGCTCGCCGACCGCATCGGCCCCGGCAAGATCGTGCTGACCGGCATCGCCGTGATCACGGTCGGCATGGGCA
TGTTCACCCAGCTCGACCAGGACACCTCCTATGCCTACCTGCTCACCGCACTGTTCGTGATGGGCCTCGGCATGGGCGCC
ACGATGATGCCGATCATGTCCGCCGCGCTCGCGACGCTGCGCGAGCACACGATCGCCCGCGGCTCGACGCTGATGAACAT
CGTCCAGCAGGTCGCCGCCTCGATCGGTACGGCGGTGTTCGCGATGCTGCTCACCAACGGCACCATCGATCTCGACCCGA
GCGACCCCGCCGGGTTCGTCACCGGCCTCGCCGACGCGTTCGCCCACGTGTTCGTGGTCGCCACGGTCATGGTCGCGCTG
GTCCTGGTCCCGGCGTTCTTCCTGCCCCGCCGCAAGGTCGCCCCGGCCGACCCCACCTTGGTGGTCGGCCACTAG

Upstream 100 bases:

>100_bases
GGTCGGCTGACTCCCCTGTCCTCACTCGGTGACCCAGCCCACAGGCCGGAGAGCCGAAAGTAGGGTGCTGAGCCCAGGTA
GGCTCACCTAGCCTCTTCCG

Downstream 100 bases:

>100_bases
TTGTCGAGCCTCGCCCCGTCACCTCCGCGGGACCACGCGAGAGGTCATACGGACGGTGGAGGGGGACACACTGTCCGTAT
GACGTCCCGCGAGCCGGCGC

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MTETQDERTAPAPGRPVDAPDAPPEGLDRRVLMVAGVVVLGAIMSILDITVVSVALQTFQQEFDATSAEVAWTMTGYTLA
LASVIPLTGWAADRFGTKRLYLLAVLLFTAGSVLCAAATSLEMLVSFRVLQGVGGGMLMPLGMTILTRAAGPERVGRVMA
VLGIPMLLGPIFGPILGGALIDSASWHWIFLINLPIGIAALAYAAVVLPRDHVEPSETFDWLGMVLLSPGLAAFLYGVSS
IPAAREEHGTLWTTEVVAPALAGLLLIAAFVPWALNRRNLHPLVDLRLFGNGTMTAAVLSMSLFAIAFFGASLLFPLYFQ
WVRGEDALGAGLLLAPQGVGAMITMPIAGVLADRIGPGKIVLTGIAVITVGMGMFTQLDQDTSYAYLLTALFVMGLGMGA
TMMPIMSAALATLREHTIARGSTLMNIVQQVAASIGTAVFAMLLTNGTIDLDPSDPAGFVTGLADAFAHVFVVATVMVAL
VLVPAFFLPRRKVAPADPTLVVGH

Sequences:

>Translated_504_residues
MTETQDERTAPAPGRPVDAPDAPPEGLDRRVLMVAGVVVLGAIMSILDITVVSVALQTFQQEFDATSAEVAWTMTGYTLA
LASVIPLTGWAADRFGTKRLYLLAVLLFTAGSVLCAAATSLEMLVSFRVLQGVGGGMLMPLGMTILTRAAGPERVGRVMA
VLGIPMLLGPIFGPILGGALIDSASWHWIFLINLPIGIAALAYAAVVLPRDHVEPSETFDWLGMVLLSPGLAAFLYGVSS
IPAAREEHGTLWTTEVVAPALAGLLLIAAFVPWALNRRNLHPLVDLRLFGNGTMTAAVLSMSLFAIAFFGASLLFPLYFQ
WVRGEDALGAGLLLAPQGVGAMITMPIAGVLADRIGPGKIVLTGIAVITVGMGMFTQLDQDTSYAYLLTALFVMGLGMGA
TMMPIMSAALATLREHTIARGSTLMNIVQQVAASIGTAVFAMLLTNGTIDLDPSDPAGFVTGLADAFAHVFVVATVMVAL
VLVPAFFLPRRKVAPADPTLVVGH
>Mature_503_residues
TETQDERTAPAPGRPVDAPDAPPEGLDRRVLMVAGVVVLGAIMSILDITVVSVALQTFQQEFDATSAEVAWTMTGYTLAL
ASVIPLTGWAADRFGTKRLYLLAVLLFTAGSVLCAAATSLEMLVSFRVLQGVGGGMLMPLGMTILTRAAGPERVGRVMAV
LGIPMLLGPIFGPILGGALIDSASWHWIFLINLPIGIAALAYAAVVLPRDHVEPSETFDWLGMVLLSPGLAAFLYGVSSI
PAAREEHGTLWTTEVVAPALAGLLLIAAFVPWALNRRNLHPLVDLRLFGNGTMTAAVLSMSLFAIAFFGASLLFPLYFQW
VRGEDALGAGLLLAPQGVGAMITMPIAGVLADRIGPGKIVLTGIAVITVGMGMFTQLDQDTSYAYLLTALFVMGLGMGAT
MMPIMSAALATLREHTIARGSTLMNIVQQVAASIGTAVFAMLLTNGTIDLDPSDPAGFVTGLADAFAHVFVVATVMVALV
LVPAFFLPRRKVAPADPTLVVGH

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=423, Percent_Identity=34.7517730496454, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1789042, Length=432, Percent_Identity=29.8611111111111, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1788710, Length=404, Percent_Identity=29.2079207920792, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1788392, Length=412, Percent_Identity=28.6407766990291, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87081983, Length=425, Percent_Identity=28, Blast_Score=132, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6322958, Length=462, Percent_Identity=24.4588744588745, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=35.3333333333333, Blast_Score=93, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6325455, Length=393, Percent_Identity=28.2442748091603, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52862; Mature: 52731

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETQDERTAPAPGRPVDAPDAPPEGLDRRVLMVAGVVVLGAIMSILDITVVSVALQTFQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEFDATSAEVAWTMTGYTLALASVIPLTGWAADRFGTKRLYLLAVLLFTAGSVLCAAATS
HHHCCCCCEEEEEEHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LEMLVSFRVLQGVGGGMLMPLGMTILTRAAGPERVGRVMAVLGIPMLLGPIFGPILGGAL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDSASWHWIFLINLPIGIAALAYAAVVLPRDHVEPSETFDWLGMVLLSPGLAAFLYGVSS
HCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHC
IPAAREEHGTLWTTEVVAPALAGLLLIAAFVPWALNRRNLHPLVDLRLFGNGTMTAAVLS
CCCCHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHH
MSLFAIAFFGASLLFPLYFQWVRGEDALGAGLLLAPQGVGAMITMPIAGVLADRIGPGKI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEEHHHHHHHHHHCCCCCHH
VLTGIAVITVGMGMFTQLDQDTSYAYLLTALFVMGLGMGATMMPIMSAALATLREHTIAR
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
GSTLMNIVQQVAASIGTAVFAMLLTNGTIDLDPSDPAGFVTGLADAFAHVFVVATVMVAL
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VLVPAFFLPRRKVAPADPTLVVGH
HHHHHHHCCCCCCCCCCCCEEECC
>Mature Secondary Structure 
TETQDERTAPAPGRPVDAPDAPPEGLDRRVLMVAGVVVLGAIMSILDITVVSVALQTFQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEFDATSAEVAWTMTGYTLALASVIPLTGWAADRFGTKRLYLLAVLLFTAGSVLCAAATS
HHHCCCCCEEEEEEHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LEMLVSFRVLQGVGGGMLMPLGMTILTRAAGPERVGRVMAVLGIPMLLGPIFGPILGGAL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDSASWHWIFLINLPIGIAALAYAAVVLPRDHVEPSETFDWLGMVLLSPGLAAFLYGVSS
HCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHC
IPAAREEHGTLWTTEVVAPALAGLLLIAAFVPWALNRRNLHPLVDLRLFGNGTMTAAVLS
CCCCHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHH
MSLFAIAFFGASLLFPLYFQWVRGEDALGAGLLLAPQGVGAMITMPIAGVLADRIGPGKI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEEHHHHHHHHHHCCCCCHH
VLTGIAVITVGMGMFTQLDQDTSYAYLLTALFVMGLGMGATMMPIMSAALATLREHTIAR
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
GSTLMNIVQQVAASIGTAVFAMLLTNGTIDLDPSDPAGFVTGLADAFAHVFVVATVMVAL
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VLVPAFFLPRRKVAPADPTLVVGH
HHHHHHHCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]