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The map label for this gene is hyuB [H]

Identifier: 119714348

GI number: 119714348

Start: 90197

End: 91915

Strand: Direct

Name: hyuB [H]

Synonym: Noca_0080

Alternate gene names: 119714348

Gene position: 90197-91915 (Clockwise)

Preceding gene: 119714347

Following gene: 119714349

Centisome position: 1.81

GC content: 67.31

Gene sequence:

>1719_bases
GTGGTTGATTCCATCACTGTCGAGGTCATCCGCCACAGCCTCCTGGCGTCGGCCGATGAGATGGCGCGCAACCTGTGCCG
GACGGCGTACAACACCGTGGTCTACGAGATCCACGACTACGGGATCGGGCTGCACGACGCGGCCGGCGACGTCGTCGCCG
ACACGCCGGGCATCGCGATGTTCACGGGGGCCAACGACGTGGGCATCCGCAACACGATCAAGTTCCTCGGCGAGGACAAC
CTCCACCCGGGCGACGTGTACTTCCTGAACTACCCGTACTGGTCGTCGGCGCACACCCTGGACGCGCTCGTGCTCGCCCC
GATCTTCGTGGGGGAGGAGCTCGTCGCGTGGTCGACCTGCCGGGTCCACCTGCTCGACCTGAAGCAGAAGGACGCGGGCT
ACGTGCTCGACTCCACCGACATGTCGCAGGAAGGCATCTTCTTCCCGTCGGTGAAGCTCTACGACCGCGGCGTGATCAAC
GACGACATCTTCAACGTGATCCGGTTCAACAGCCGGATGCCCGAGCGGACCATCGGCGACATCCAGGCACAGGTCTCGGC
CTGTCAGACCGGGGAACGCCGGGTCCAGGAGATCGCGGCCAAGCACGGCGTGGACGTCCTGCGGGAGGCGATGCGCGAGA
TCAACGACCATGGCGAGCGCCTGGCGAAGGCCGCGTTGGCGAAGCTGCCGAAGGGCACCTGGTCGGCGTACGACTTCGTC
GACACCGACGGCGTCGTCAAGGACGAGATGGTCCGGATGGACGTCACGGTCACCGTCACCGACGAGAAGATGATCGTCGA
CTGGCGGGAGACCTCCAGGCCGGCCAAGGGCCCCATCAACCTGCCGCGTGGTCTCACGGTGGCCACGACGATGATGGCCT
TCAAGGCGCTCACCACGCCGGACACTCCGGTGAACTCCGGCAACTTCCGTCCGGTCGAGGTGCTCACGACGCCCGGTTCG
CTGATGCATGCGGTTGAGCCGATGCCGACGTTCACGATCTGGCCGGCGATCCTCGCTCCGGAGGTCATCAACAAGGCGCT
CGCCCAGGGGATGCCGGACCTGGTCCCGGCGTGCTCGGGTGGGGACGTCTGCTCGATCATGGCGCTCGGGGTCGACCCCG
AGACGGGGGTCTCGTGGCTGGAGGCCACGAACGACGGGGTCGGTTTCGGCGCGCACTCGGGCGGCGACGGGTCGGACGGC
ATCATGCACCTCTCGGAGCCGGGGTGTCGGAACAACCCGATCGAGATCCTGGAGACCAAGGCGCCCATGATCATCGAGAA
CTACCGGTACCGCACCGACTCCGGAGGTCCCGGGAAGTACCGCGGTGGCGTCGGCGTGGAACGCACCTACCGCTTCCTGG
CCCCGTCCTCGGCGATCGTCATCAACTACAAGACCAAGACGAAGCCGTGGGGCATCGGCGAGGGTCGTGACGGCCTGAAC
AACACGGTCGTGCTCTACCCGGGGACCGACCGCTCCGTCGAGGTCGGCCAGAGCTACACGCACTTCGCGACCAACGAGTG
CATGGCCAACGTCACCGGCGGCGGAGGGGGATGGGGCGACCCGTTCACGCGTGACCCGGCCGCCGTCGCCACCGACGTGC
GGCTCGGGTTCGTCAGCGTCGAGCGTGCCGCGAGCGACTACGGCGTCGTGGTCGACCCGGCGACGTACGACGTCGACGAG
GAGGCCACCCGTCGGTTGCGCTCGGTCGGTGCGTCGTGA

Upstream 100 bases:

>100_bases
ATCGTCGAGCACACCTCGACGACGGTCATGCACACCGGCGACACGGCCGAGGTCGGCCGTGTCGGAGAGATCGTCATCAC
GCTTGCAGAGGAGACGAACC

Downstream 100 bases:

>100_bases
GCGAGCCGAGGCCGCGCCCGGCCGCGCGCGAGCCGCTCGTGCTCGACGCGGCCGTGCCCCTCGTCTCGCATCCCCGGCAC
CTGTCGGCTCTGCTCCCTGC

Product: 5-oxoprolinase

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MVDSITVEVIRHSLLASADEMARNLCRTAYNTVVYEIHDYGIGLHDAAGDVVADTPGIAMFTGANDVGIRNTIKFLGEDN
LHPGDVYFLNYPYWSSAHTLDALVLAPIFVGEELVAWSTCRVHLLDLKQKDAGYVLDSTDMSQEGIFFPSVKLYDRGVIN
DDIFNVIRFNSRMPERTIGDIQAQVSACQTGERRVQEIAAKHGVDVLREAMREINDHGERLAKAALAKLPKGTWSAYDFV
DTDGVVKDEMVRMDVTVTVTDEKMIVDWRETSRPAKGPINLPRGLTVATTMMAFKALTTPDTPVNSGNFRPVEVLTTPGS
LMHAVEPMPTFTIWPAILAPEVINKALAQGMPDLVPACSGGDVCSIMALGVDPETGVSWLEATNDGVGFGAHSGGDGSDG
IMHLSEPGCRNNPIEILETKAPMIIENYRYRTDSGGPGKYRGGVGVERTYRFLAPSSAIVINYKTKTKPWGIGEGRDGLN
NTVVLYPGTDRSVEVGQSYTHFATNECMANVTGGGGGWGDPFTRDPAAVATDVRLGFVSVERAASDYGVVVDPATYDVDE
EATRRLRSVGAS

Sequences:

>Translated_572_residues
MVDSITVEVIRHSLLASADEMARNLCRTAYNTVVYEIHDYGIGLHDAAGDVVADTPGIAMFTGANDVGIRNTIKFLGEDN
LHPGDVYFLNYPYWSSAHTLDALVLAPIFVGEELVAWSTCRVHLLDLKQKDAGYVLDSTDMSQEGIFFPSVKLYDRGVIN
DDIFNVIRFNSRMPERTIGDIQAQVSACQTGERRVQEIAAKHGVDVLREAMREINDHGERLAKAALAKLPKGTWSAYDFV
DTDGVVKDEMVRMDVTVTVTDEKMIVDWRETSRPAKGPINLPRGLTVATTMMAFKALTTPDTPVNSGNFRPVEVLTTPGS
LMHAVEPMPTFTIWPAILAPEVINKALAQGMPDLVPACSGGDVCSIMALGVDPETGVSWLEATNDGVGFGAHSGGDGSDG
IMHLSEPGCRNNPIEILETKAPMIIENYRYRTDSGGPGKYRGGVGVERTYRFLAPSSAIVINYKTKTKPWGIGEGRDGLN
NTVVLYPGTDRSVEVGQSYTHFATNECMANVTGGGGGWGDPFTRDPAAVATDVRLGFVSVERAASDYGVVVDPATYDVDE
EATRRLRSVGAS
>Mature_572_residues
MVDSITVEVIRHSLLASADEMARNLCRTAYNTVVYEIHDYGIGLHDAAGDVVADTPGIAMFTGANDVGIRNTIKFLGEDN
LHPGDVYFLNYPYWSSAHTLDALVLAPIFVGEELVAWSTCRVHLLDLKQKDAGYVLDSTDMSQEGIFFPSVKLYDRGVIN
DDIFNVIRFNSRMPERTIGDIQAQVSACQTGERRVQEIAAKHGVDVLREAMREINDHGERLAKAALAKLPKGTWSAYDFV
DTDGVVKDEMVRMDVTVTVTDEKMIVDWRETSRPAKGPINLPRGLTVATTMMAFKALTTPDTPVNSGNFRPVEVLTTPGS
LMHAVEPMPTFTIWPAILAPEVINKALAQGMPDLVPACSGGDVCSIMALGVDPETGVSWLEATNDGVGFGAHSGGDGSDG
IMHLSEPGCRNNPIEILETKAPMIIENYRYRTDSGGPGKYRGGVGVERTYRFLAPSSAIVINYKTKTKPWGIGEGRDGLN
NTVVLYPGTDRSVEVGQSYTHFATNECMANVTGGGGGWGDPFTRDPAAVATDVRLGFVSVERAASDYGVVVDPATYDVDE
EATRRLRSVGAS

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0146

COG function: function code EQ; N-methylhydantoinase B/acetone carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=543, Percent_Identity=27.255985267035, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI133901900, Length=545, Percent_Identity=25.6880733944954, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI133901902, Length=508, Percent_Identity=25.1968503937008, Blast_Score=140, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6322634, Length=546, Percent_Identity=24.9084249084249, Blast_Score=147, Evalue=4e-36,
Organism=Drosophila melanogaster, GI45550492, Length=558, Percent_Identity=23.6559139784946, Blast_Score=135, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003692 [H]

Pfam domain/function: PF02538 Hydantoinase_B [H]

EC number: 3.5.2.14

Molecular weight: Translated: 61935; Mature: 61935

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDSITVEVIRHSLLASADEMARNLCRTAYNTVVYEIHDYGIGLHDAAGDVVADTPGIAM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEEECCCCEEE
FTGANDVGIRNTIKFLGEDNLHPGDVYFLNYPYWSSAHTLDALVLAPIFVGEELVAWSTC
EECCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHEEHEE
RVHLLDLKQKDAGYVLDSTDMSQEGIFFPSVKLYDRGVINDDIFNVIRFNSRMPERTIGD
EEEEEECCCCCCCEEEECCCCCCCCEECCCCHHHCCCCCCHHHHHHHHHCCCCCCHHHHH
IQAQVSACQTGERRVQEIAAKHGVDVLREAMREINDHGERLAKAALAKLPKGTWSAYDFV
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
DTDGVVKDEMVRMDVTVTVTDEKMIVDWRETSRPAKGPINLPRGLTVATTMMAFKALTTP
CCCCCCCCCEEEEEEEEEEECCEEEEEEHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
DTPVNSGNFRPVEVLTTPGSLMHAVEPMPTFTIWPAILAPEVINKALAQGMPDLVPACSG
CCCCCCCCCEEEEEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GDVCSIMALGVDPETGVSWLEATNDGVGFGAHSGGDGSDGIMHLSEPGCRNNPIEILETK
CCEEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHEEECC
APMIIENYRYRTDSGGPGKYRGGVGVERTYRFLAPSSAIVINYKTKTKPWGIGEGRDGLN
CCEEEECCEEECCCCCCCCCCCCCCHHHHHHEECCCCEEEEEEECCCCCCCCCCCCCCCC
NTVVLYPGTDRSVEVGQSYTHFATNECMANVTGGGGGWGDPFTRDPAAVATDVRLGFVSV
CEEEEECCCCCCEECCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHEEHHEEEEEEH
ERAASDYGVVVDPATYDVDEEATRRLRSVGAS
HHCCCCCCEEECCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MVDSITVEVIRHSLLASADEMARNLCRTAYNTVVYEIHDYGIGLHDAAGDVVADTPGIAM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEEECCCCEEE
FTGANDVGIRNTIKFLGEDNLHPGDVYFLNYPYWSSAHTLDALVLAPIFVGEELVAWSTC
EECCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHEEHEE
RVHLLDLKQKDAGYVLDSTDMSQEGIFFPSVKLYDRGVINDDIFNVIRFNSRMPERTIGD
EEEEEECCCCCCCEEEECCCCCCCCEECCCCHHHCCCCCCHHHHHHHHHCCCCCCHHHHH
IQAQVSACQTGERRVQEIAAKHGVDVLREAMREINDHGERLAKAALAKLPKGTWSAYDFV
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
DTDGVVKDEMVRMDVTVTVTDEKMIVDWRETSRPAKGPINLPRGLTVATTMMAFKALTTP
CCCCCCCCCEEEEEEEEEEECCEEEEEEHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
DTPVNSGNFRPVEVLTTPGSLMHAVEPMPTFTIWPAILAPEVINKALAQGMPDLVPACSG
CCCCCCCCCEEEEEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GDVCSIMALGVDPETGVSWLEATNDGVGFGAHSGGDGSDGIMHLSEPGCRNNPIEILETK
CCEEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHEEECC
APMIIENYRYRTDSGGPGKYRGGVGVERTYRFLAPSSAIVINYKTKTKPWGIGEGRDGLN
CCEEEECCEEECCCCCCCCCCCCCCHHHHHHEECCCCEEEEEEECCCCCCCCCCCCCCCC
NTVVLYPGTDRSVEVGQSYTHFATNECMANVTGGGGGWGDPFTRDPAAVATDVRLGFVSV
CEEEEECCCCCCEECCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHEEHHEEEEEEH
ERAASDYGVVVDPATYDVDEEATRRLRSVGAS
HHCCCCCCEEECCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]