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The map label for this gene is lhgO [H]

Identifier: 119714306

GI number: 119714306

Start: 37757

End: 38983

Strand: Direct

Name: lhgO [H]

Synonym: Noca_0034

Alternate gene names: 119714306

Gene position: 37757-38983 (Clockwise)

Preceding gene: 119714305

Following gene: 119714307

Centisome position: 0.76

GC content: 73.84

Gene sequence:

>1227_bases
GTGAGCGGGAGCCCGCGCCGACCCCGTGTCGGGGTCGTGGGCGGCGGGATCGTGGGCGCGGCCGTCGCGCGCGAGCTGCT
CGCCCGGTACGACGCCGAGGTCGTCCTCTGGGAGAAGGAGGACGGCCTCGGCCGGCATCAGACCGGCCACAACAGCGGCG
TCGTCCACGCCGGTGTCTACTACACCCCCGGGTCTCACAAGGCACGCCTGTGCGTCCAGGGCGGTCGGTTGCTGCGGGCC
TTCTGCGAGGAGCGCGGCCTGGTCTACGAGGAGTGCGGGAAGCTCATCGTCGCCACCGACGAAGGTCAGCGAAGTCTGCT
GCACGACATCGAGCGGCGAGGTCGGAGCAACGGTGTGACCGGGCTGCGGTGGCTCGAGGGCGAGGAGATCGGATCGGTCG
AGCCGCACGCTCGCGGTGTCGCCGCACTGCACTCTCCTCGCACCGCGATCGTCGACTACGGCGCGGTCACCCGAGCGCTG
GGAGCCGAGGTCGTCGACCGCGGCGGCCTGCTCCACGTCGGGACGGCGGTCACGGGCCTGCAGACCCGGGGGCGTGAGGT
CGTCGTGACCACGTCCGCGGGCTCACAGGTCGTCGACCTGGTGGTGGCGTGCGCCGGGCTCCATGCCGACCGGGTCGCCC
GGATGGCCGGCGACGACTCCGAGCCGATGATCGTTCCGTTCCGCGGCGAGTACCACGTCCTGCGCCCCGACCGCAGCGCG
CTGGTGCGCGGCATGATCTACCCGGTGCCGGATCCCCGCTATCCCTTCCTCGGCGTGCACCTGACCCGGCACGTCGACGG
GTCGGTGTCGATCGGCCCCAACGCCGTCCTGGCACTCGCCCGGGAGGGCTACCGCTGGCGCGACGTCGCTGGGCGCGACC
TCTGGGAGATGGCTCGGTGGCCGGGCTTCGCCCGCCTCGCGCGACGGCACTGGCGCACCGGCGTGCGCGAGCTCGCCGGC
TCGGCCAGCAAGCGGCGGTTCGTGGCGGCGGCCCGTCTCTACGTGCCCGAGCTGCGGGTGGCGGACGTCGAGCGCCGAGT
CGCGGGTGTCCGTGCGCAGGCCGTGGACCGGGACGGGACGCTTGTCGACGACTTCCGCATCGGCCGCGCCGGCCGGGTGC
TGAGCGTGCGCAACGCACCGTCGCCCGCGGCGACCTCCTCGCTCGCGATCGCCGCCGAGGTCGTCGCCATGCTGGCCGAC
GATCTCCCGCGCCGGTCCGGAGGCTGA

Upstream 100 bases:

>100_bases
CGGTGGTGCGGCCGCCGCTCCGGCCGCTGCCGGAGGACCACGACGCGGAGCTGGGGCTCATCCTCGAGGGATGGAAGAAC
TCGCCACGACCGGGGGCCGG

Downstream 100 bases:

>100_bases
AGGCCCCACTCCGGCGTGTATTCTGCTGACAAGAGACAACTGGGTCTCGCGGGCGGCCGTGACGTCGCTACCTCAGGGCG
GTCCGGGCCATGGGCCCACC

Product: hydroxyglutarate oxidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MSGSPRRPRVGVVGGGIVGAAVARELLARYDAEVVLWEKEDGLGRHQTGHNSGVVHAGVYYTPGSHKARLCVQGGRLLRA
FCEERGLVYEECGKLIVATDEGQRSLLHDIERRGRSNGVTGLRWLEGEEIGSVEPHARGVAALHSPRTAIVDYGAVTRAL
GAEVVDRGGLLHVGTAVTGLQTRGREVVVTTSAGSQVVDLVVACAGLHADRVARMAGDDSEPMIVPFRGEYHVLRPDRSA
LVRGMIYPVPDPRYPFLGVHLTRHVDGSVSIGPNAVLALAREGYRWRDVAGRDLWEMARWPGFARLARRHWRTGVRELAG
SASKRRFVAAARLYVPELRVADVERRVAGVRAQAVDRDGTLVDDFRIGRAGRVLSVRNAPSPAATSSLAIAAEVVAMLAD
DLPRRSGG

Sequences:

>Translated_408_residues
MSGSPRRPRVGVVGGGIVGAAVARELLARYDAEVVLWEKEDGLGRHQTGHNSGVVHAGVYYTPGSHKARLCVQGGRLLRA
FCEERGLVYEECGKLIVATDEGQRSLLHDIERRGRSNGVTGLRWLEGEEIGSVEPHARGVAALHSPRTAIVDYGAVTRAL
GAEVVDRGGLLHVGTAVTGLQTRGREVVVTTSAGSQVVDLVVACAGLHADRVARMAGDDSEPMIVPFRGEYHVLRPDRSA
LVRGMIYPVPDPRYPFLGVHLTRHVDGSVSIGPNAVLALAREGYRWRDVAGRDLWEMARWPGFARLARRHWRTGVRELAG
SASKRRFVAAARLYVPELRVADVERRVAGVRAQAVDRDGTLVDDFRIGRAGRVLSVRNAPSPAATSSLAIAAEVVAMLAD
DLPRRSGG
>Mature_407_residues
SGSPRRPRVGVVGGGIVGAAVARELLARYDAEVVLWEKEDGLGRHQTGHNSGVVHAGVYYTPGSHKARLCVQGGRLLRAF
CEERGLVYEECGKLIVATDEGQRSLLHDIERRGRSNGVTGLRWLEGEEIGSVEPHARGVAALHSPRTAIVDYGAVTRALG
AEVVDRGGLLHVGTAVTGLQTRGREVVVTTSAGSQVVDLVVACAGLHADRVARMAGDDSEPMIVPFRGEYHVLRPDRSAL
VRGMIYPVPDPRYPFLGVHLTRHVDGSVSIGPNAVLALAREGYRWRDVAGRDLWEMARWPGFARLARRHWRTGVRELAGS
ASKRRFVAAARLYVPELRVADVERRVAGVRAQAVDRDGTLVDDFRIGRAGRVLSVRNAPSPAATSSLAIAAEVVAMLADD
LPRRSGG

Specific function: Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate [H]

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L2HGDH family [H]

Homologues:

Organism=Homo sapiens, GI13376331, Length=403, Percent_Identity=42.6799007444169, Blast_Score=334, Evalue=8e-92,
Organism=Escherichia coli, GI87082148, Length=389, Percent_Identity=42.159383033419, Blast_Score=316, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI17565764, Length=413, Percent_Identity=43.0992736077482, Blast_Score=332, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24585081, Length=398, Percent_Identity=46.9849246231156, Blast_Score=352, Evalue=3e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015657
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.1.3.15 [H]

Molecular weight: Translated: 44012; Mature: 43881

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGSPRRPRVGVVGGGIVGAAVARELLARYDAEVVLWEKEDGLGRHQTGHNSGVVHAGVY
CCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEEEE
YTPGSHKARLCVQGGRLLRAFCEERGLVYEECGKLIVATDEGQRSLLHDIERRGRSNGVT
ECCCCCCEEEEECCHHHHHHHHHHCCCCHHHCCCEEEEECCCHHHHHHHHHHCCCCCCCC
GLRWLEGEEIGSVEPHARGVAALHSPRTAIVDYGAVTRALGAEVVDRGGLLHVGTAVTGL
CEEEECCCCCCCCCCCCCCHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCEEEECHHHHHH
QTRGREVVVTTSAGSQVVDLVVACAGLHADRVARMAGDDSEPMIVPFRGEYHVLRPDRSA
HCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEECCCEEEECCCHHH
LVRGMIYPVPDPRYPFLGVHLTRHVDGSVSIGPNAVLALAREGYRWRDVAGRDLWEMARW
HHHCCEECCCCCCCCEEEEEEEEECCCCEEECCHHHHHHHHCCCEEECCCCCHHHHHHHC
PGFARLARRHWRTGVRELAGSASKRRFVAAARLYVPELRVADVERRVAGVRAQAVDRDGT
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEECCCCC
LVDDFRIGRAGRVLSVRNAPSPAATSSLAIAAEVVAMLADDLPRRSGG
EEECEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SGSPRRPRVGVVGGGIVGAAVARELLARYDAEVVLWEKEDGLGRHQTGHNSGVVHAGVY
CCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEEEE
YTPGSHKARLCVQGGRLLRAFCEERGLVYEECGKLIVATDEGQRSLLHDIERRGRSNGVT
ECCCCCCEEEEECCHHHHHHHHHHCCCCHHHCCCEEEEECCCHHHHHHHHHHCCCCCCCC
GLRWLEGEEIGSVEPHARGVAALHSPRTAIVDYGAVTRALGAEVVDRGGLLHVGTAVTGL
CEEEECCCCCCCCCCCCCCHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCEEEECHHHHHH
QTRGREVVVTTSAGSQVVDLVVACAGLHADRVARMAGDDSEPMIVPFRGEYHVLRPDRSA
HCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEECCCEEEECCCHHH
LVRGMIYPVPDPRYPFLGVHLTRHVDGSVSIGPNAVLALAREGYRWRDVAGRDLWEMARW
HHHCCEECCCCCCCCEEEEEEEEECCCCEEECCHHHHHHHHCCCEEECCCCCHHHHHHHC
PGFARLARRHWRTGVRELAGSASKRRFVAAARLYVPELRVADVERRVAGVRAQAVDRDGT
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEECCCCC
LVDDFRIGRAGRVLSVRNAPSPAATSSLAIAAEVVAMLADDLPRRSGG
EEECEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8297211; 7984428 [H]