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The map label for this gene is dnaB [H]

Identifier: 119387174

GI number: 119387174

Start: 1682979

End: 1684478

Strand: Direct

Name: dnaB [H]

Synonym: Pden_4469

Alternate gene names: 119387174

Gene position: 1682979-1684478 (Clockwise)

Preceding gene: 119387173

Following gene: 119387175

Centisome position: 97.28

GC content: 66.67

Gene sequence:

>1500_bases
ATGAGCGAGTTGCGCGCCGTCGAAGTCAGGAAGCCCGGCGAGATCGCCGAGGCCGCCGCCGCGCAGGCGGTGCCCTTCTC
GATCGAGGCCGAGCAGCAGTTGCTGGGTGCGCTCCTGACCAATAACGAGGTCTACGACCACGTTTCCCGCATCATCCAGG
CGCGGCATTTCTACGATCCGGTCCATCGACGCATCTACGAGATCTGCGCCGAGCGCATCGCCAGGAACGCGCTGGCAAGC
CCCGTGACGATCAAGGCCTTCATGGAGCACGACGCCGGGCTCAAGGAATTGGGCGGCCCGGCCTATCTGGCGCGGCTGGC
GGGGGCGGCGATCTCGTCCCATGCCGCACGCGACTATGCGCAGATGATCCGCGAATTCGCCCTGCGCCGCGAGTTGATCG
GGCTGGGCCAGGACATCGCCGCGCGTGCCTCGACCGTCTCGGTCAGCGATTCCGCCGAGGAGCAGATCAAGGAAGCCGAG
CAGGTCCTCTACAAGCTCGGCGAGCAGGGCGTGGCCGAACGCGGGTTCCAGAGTTTCCTTGCCGCCGTCACCGGGGCCCT
GAACGCCGCCAATGCCGCCTTCAGCCGGGGCGGCGGGCTGTCGGGCGTCTCGACCGGGCTGGTCGACCTCGACGGCAAGA
TGGGCGGGTTGAACCGATCGGACCTGATCATCCTGGCCGGCCGGCCCTCGATGGGCAAGACCTCGCTGGCGACCAACATC
GCCTTCAACGTCGCCAAGTCGCACAAGCTGGGCGAATTGCCCGACGGCAGCCATGGCACGGTGGCGGGGGGCGTCGTCGG
CTTCTTCAGCCTGGAGATGTCGGCAGAGCAGCTGGCCGCGCGCATCCTGTCCGAAGCCGCCGAGGTTCCCAGCGAGGCGA
TCCGCCGCGGCGACATGACCGAGGACGAGTTCCGCCGCTTCGTCAAGGCCGCGCATGACTTGCAGAACTGCCCGCTTTAC
ATCGACGATACGCCGGCACTGCCGATCAATCAGTTGGCCGCGCGTGCCCGCAAGCTCAAGCGCACCATGGGGCTGGACCT
GCTGATCGTGGACTATCTCCAGCTTCTGCGCGCAGCCTCGGCCAAGGACAGCCGCGTCAACGAGGTCAGCGAGATCACCC
AGGGCCTCAAGGCCGTCGCCAAGGAACTGGACATCCCCGTCATCGCCCTGTCGCAGCTGTCGCGCCAGGTCGAGGCGCGC
GAGGACAAGCGCCCGCAGCTTTCCGACCTGCGCGAATCCGGCAGCATCGAGCAGGACGCCGACATCGTGATGTTCGTCTT
CCGCGAGGAATATTACCGCGAGCGCGAGAAGCCCGCCGACCACGACCTCGACAAGATGGCCACCTGGCAGCAGGTCATGG
AATCCTGCCACGGCAAGGCCGAGGTCATCATCGGCAAGCAGCGCCACGGCCCCATCGGCACGGTCGAGCTGTCCTTCGAG
GGCCGCTTCACCCGCTTCGGAAACCTGGAAAAACACCGCAGCTGGGATCCTGCCGAATGA

Upstream 100 bases:

>100_bases
GCAATTCGCCCACAGCTTATCCACAGCATATCCCGATGGACCCCGGGGCGCATAAGGTGGATAACGACCCCCAGCCGAGT
CGGACGTCGGAGAGCATGGG

Downstream 100 bases:

>100_bases
ACCTGCCGGTGCCTTTCGTCACCATCGGCCTGCTGATCCTGTCGAACCTGTTCATGACCGTCGCCTGGTACGGGCATCTG
AAGTTCAAGGCCGCGCCGCT

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MSELRAVEVRKPGEIAEAAAAQAVPFSIEAEQQLLGALLTNNEVYDHVSRIIQARHFYDPVHRRIYEICAERIARNALAS
PVTIKAFMEHDAGLKELGGPAYLARLAGAAISSHAARDYAQMIREFALRRELIGLGQDIAARASTVSVSDSAEEQIKEAE
QVLYKLGEQGVAERGFQSFLAAVTGALNAANAAFSRGGGLSGVSTGLVDLDGKMGGLNRSDLIILAGRPSMGKTSLATNI
AFNVAKSHKLGELPDGSHGTVAGGVVGFFSLEMSAEQLAARILSEAAEVPSEAIRRGDMTEDEFRRFVKAAHDLQNCPLY
IDDTPALPINQLAARARKLKRTMGLDLLIVDYLQLLRAASAKDSRVNEVSEITQGLKAVAKELDIPVIALSQLSRQVEAR
EDKRPQLSDLRESGSIEQDADIVMFVFREEYYREREKPADHDLDKMATWQQVMESCHGKAEVIIGKQRHGPIGTVELSFE
GRFTRFGNLEKHRSWDPAE

Sequences:

>Translated_499_residues
MSELRAVEVRKPGEIAEAAAAQAVPFSIEAEQQLLGALLTNNEVYDHVSRIIQARHFYDPVHRRIYEICAERIARNALAS
PVTIKAFMEHDAGLKELGGPAYLARLAGAAISSHAARDYAQMIREFALRRELIGLGQDIAARASTVSVSDSAEEQIKEAE
QVLYKLGEQGVAERGFQSFLAAVTGALNAANAAFSRGGGLSGVSTGLVDLDGKMGGLNRSDLIILAGRPSMGKTSLATNI
AFNVAKSHKLGELPDGSHGTVAGGVVGFFSLEMSAEQLAARILSEAAEVPSEAIRRGDMTEDEFRRFVKAAHDLQNCPLY
IDDTPALPINQLAARARKLKRTMGLDLLIVDYLQLLRAASAKDSRVNEVSEITQGLKAVAKELDIPVIALSQLSRQVEAR
EDKRPQLSDLRESGSIEQDADIVMFVFREEYYREREKPADHDLDKMATWQQVMESCHGKAEVIIGKQRHGPIGTVELSFE
GRFTRFGNLEKHRSWDPAE
>Mature_498_residues
SELRAVEVRKPGEIAEAAAAQAVPFSIEAEQQLLGALLTNNEVYDHVSRIIQARHFYDPVHRRIYEICAERIARNALASP
VTIKAFMEHDAGLKELGGPAYLARLAGAAISSHAARDYAQMIREFALRRELIGLGQDIAARASTVSVSDSAEEQIKEAEQ
VLYKLGEQGVAERGFQSFLAAVTGALNAANAAFSRGGGLSGVSTGLVDLDGKMGGLNRSDLIILAGRPSMGKTSLATNIA
FNVAKSHKLGELPDGSHGTVAGGVVGFFSLEMSAEQLAARILSEAAEVPSEAIRRGDMTEDEFRRFVKAAHDLQNCPLYI
DDTPALPINQLAARARKLKRTMGLDLLIVDYLQLLRAASAKDSRVNEVSEITQGLKAVAKELDIPVIALSQLSRQVEARE
DKRPQLSDLRESGSIEQDADIVMFVFREEYYREREKPADHDLDKMATWQQVMESCHGKAEVIIGKQRHGPIGTVELSFEG
RFTRFGNLEKHRSWDPAE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=476, Percent_Identity=38.2352941176471, Blast_Score=289, Evalue=3e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 54544; Mature: 54412

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELRAVEVRKPGEIAEAAAAQAVPFSIEAEQQLLGALLTNNEVYDHVSRIIQARHFYDP
CCCCCEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VHRRIYEICAERIARNALASPVTIKAFMEHDAGLKELGGPAYLARLAGAAISSHAARDYA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHH
QMIREFALRRELIGLGQDIAARASTVSVSDSAEEQIKEAEQVLYKLGEQGVAERGFQSFL
HHHHHHHHHHHHHHCCHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVTGALNAANAAFSRGGGLSGVSTGLVDLDGKMGGLNRSDLIILAGRPSMGKTSLATNI
HHHHHHHHHHHHHHHCCCCCCCHHCCCEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHH
AFNVAKSHKLGELPDGSHGTVAGGVVGFFSLEMSAEQLAARILSEAAEVPSEAIRRGDMT
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCC
EDEFRRFVKAAHDLQNCPLYIDDTPALPINQLAARARKLKRTMGLDLLIVDYLQLLRAAS
HHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
AKDSRVNEVSEITQGLKAVAKELDIPVIALSQLSRQVEAREDKRPQLSDLRESGSIEQDA
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCC
DIVMFVFREEYYREREKPADHDLDKMATWQQVMESCHGKAEVIIGKQRHGPIGTVELSFE
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEEC
GRFTRFGNLEKHRSWDPAE
CCCHHCCCCHHHCCCCCCC
>Mature Secondary Structure 
SELRAVEVRKPGEIAEAAAAQAVPFSIEAEQQLLGALLTNNEVYDHVSRIIQARHFYDP
CCCCEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VHRRIYEICAERIARNALASPVTIKAFMEHDAGLKELGGPAYLARLAGAAISSHAARDYA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHH
QMIREFALRRELIGLGQDIAARASTVSVSDSAEEQIKEAEQVLYKLGEQGVAERGFQSFL
HHHHHHHHHHHHHHCCHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVTGALNAANAAFSRGGGLSGVSTGLVDLDGKMGGLNRSDLIILAGRPSMGKTSLATNI
HHHHHHHHHHHHHHHCCCCCCCHHCCCEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHH
AFNVAKSHKLGELPDGSHGTVAGGVVGFFSLEMSAEQLAARILSEAAEVPSEAIRRGDMT
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCC
EDEFRRFVKAAHDLQNCPLYIDDTPALPINQLAARARKLKRTMGLDLLIVDYLQLLRAAS
HHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
AKDSRVNEVSEITQGLKAVAKELDIPVIALSQLSRQVEAREDKRPQLSDLRESGSIEQDA
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCC
DIVMFVFREEYYREREKPADHDLDKMATWQQVMESCHGKAEVIIGKQRHGPIGTVELSFE
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEEC
GRFTRFGNLEKHRSWDPAE
CCCHHCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA