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The map label for this gene is pcaR [H]

Identifier: 119386985

GI number: 119386985

Start: 1454824

End: 1455588

Strand: Direct

Name: pcaR [H]

Synonym: Pden_4280

Alternate gene names: 119386985

Gene position: 1454824-1455588 (Clockwise)

Preceding gene: 119386979

Following gene: 119386992

Centisome position: 84.09

GC content: 65.49

Gene sequence:

>765_bases
ATGACGAGTATTCAGGACAAGCCCTCCCTGCCGCCGAGCTTTCGGAATCTTCAGATTCTTGAGGTTCTTGCCAGCGAAGG
GCGGCCCCTGACGGCGACCGAGATCAACGCCGCGCTGGGGCTGCCGGTGCCGACCATCCACCGGCTGGTCGGCAACCTGG
AAGCCGAGGGTTTCCTGAGCCGCCATATCGACGGGCGCAGCTATCAGCCGGGGCCGAAACTGCGGCAGATGATGCAGGGG
GTGATCCGCTTCTGGCATCAGAACCTGCCGCAGCGCGACGTGCTGATTCGGCTGAACCAGCGCCTGGGCGAGACCTGCAA
CCTGTCGATCCCCGACGGCGATGCGATGCTTTACGTCGACCGGGTCGAGACGCATTGGCCCTTGCGGATCCAGCTTCACG
TCGGCTCGCGCGTGCCGCTGCATGCCACGGCGGCGGGCAAGCTGTGCCTGTCGCTGATCAACGACGGCAAGCTTGAACGC
TATCTGAAACGCGCCCATCTGACGGCTCATACCGGGCAGACGATCACCGACCCGGACAGGCTGCGCGAGGCGCTGCGCCA
GATCCGGCAGCAGGGTTATTCGACGGATTCCGAGGAATTCGTGCCCGGAATGATCGCCGTGGCCGTGCCCGTGCTGGACC
GCTCGGGCCAGTTGGTCGCGACGGTGTCCTTTCACGCCCCGGTCCAACGGCTGACCCTGGCCGAAGGGCTGAAGCACCTG
CCCGACCTGCGCGCCGCCGCCGAAGAGTTGACGCAGCTGATATAG

Upstream 100 bases:

>100_bases
AATACTCTCATTTTCGATTGTTCATATCTTGATCCGGGCGGTTGGAGCCACGCTTGCAGCGATGTAGAGTCGGACCGGGA
ACCGATGGAAAAGCACGATC

Downstream 100 bases:

>100_bases
GCGATCCGGGCCGCTGACAGGCAGGGGTGGCCATGTCTTGACCGCCCCGCACCGGATCGACAGGGCGCGGCATACCATCC
CGACGTGATCGAGGCGGCGG

Product: regulatory proteins, IclR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTSIQDKPSLPPSFRNLQILEVLASEGRPLTATEINAALGLPVPTIHRLVGNLEAEGFLSRHIDGRSYQPGPKLRQMMQG
VIRFWHQNLPQRDVLIRLNQRLGETCNLSIPDGDAMLYVDRVETHWPLRIQLHVGSRVPLHATAAGKLCLSLINDGKLER
YLKRAHLTAHTGQTITDPDRLREALRQIRQQGYSTDSEEFVPGMIAVAVPVLDRSGQLVATVSFHAPVQRLTLAEGLKHL
PDLRAAAEELTQLI

Sequences:

>Translated_254_residues
MTSIQDKPSLPPSFRNLQILEVLASEGRPLTATEINAALGLPVPTIHRLVGNLEAEGFLSRHIDGRSYQPGPKLRQMMQG
VIRFWHQNLPQRDVLIRLNQRLGETCNLSIPDGDAMLYVDRVETHWPLRIQLHVGSRVPLHATAAGKLCLSLINDGKLER
YLKRAHLTAHTGQTITDPDRLREALRQIRQQGYSTDSEEFVPGMIAVAVPVLDRSGQLVATVSFHAPVQRLTLAEGLKHL
PDLRAAAEELTQLI
>Mature_253_residues
TSIQDKPSLPPSFRNLQILEVLASEGRPLTATEINAALGLPVPTIHRLVGNLEAEGFLSRHIDGRSYQPGPKLRQMMQGV
IRFWHQNLPQRDVLIRLNQRLGETCNLSIPDGDAMLYVDRVETHWPLRIQLHVGSRVPLHATAAGKLCLSLINDGKLERY
LKRAHLTAHTGQTITDPDRLREALRQIRQQGYSTDSEEFVPGMIAVAVPVLDRSGQLVATVSFHAPVQRLTLAEGLKHLP
DLRAAAEELTQLI

Specific function: Positive regulator of all genes within the pca regulon, pcaBDC, pcaIJ and pcaF. Also required for the chemotactic response to aromatic compounds [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=228, Percent_Identity=30.2631578947368, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788131, Length=247, Percent_Identity=27.1255060728745, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI87082359, Length=222, Percent_Identity=29.2792792792793, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1786715, Length=225, Percent_Identity=30.6666666666667, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1789999, Length=261, Percent_Identity=26.8199233716475, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788581, Length=219, Percent_Identity=26.9406392694064, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790752, Length=215, Percent_Identity=24.6511627906977, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012794
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 28304; Mature: 28173

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIQDKPSLPPSFRNLQILEVLASEGRPLTATEINAALGLPVPTIHRLVGNLEAEGFLS
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHCCCHHHHHH
RHIDGRSYQPGPKLRQMMQGVIRFWHQNLPQRDVLIRLNQRLGETCNLSIPDGDAMLYVD
HCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCCCCEEEEEE
RVETHWPLRIQLHVGSRVPLHATAAGKLCLSLINDGKLERYLKRAHLTAHTGQTITDPDR
ECCCCCCEEEEEEECCCCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHH
LREALRQIRQQGYSTDSEEFVPGMIAVAVPVLDRSGQLVATVSFHAPVQRLTLAEGLKHL
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHC
PDLRAAAEELTQLI
HHHHHHHHHHHHHC
>Mature Secondary Structure 
TSIQDKPSLPPSFRNLQILEVLASEGRPLTATEINAALGLPVPTIHRLVGNLEAEGFLS
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHCCCHHHHHH
RHIDGRSYQPGPKLRQMMQGVIRFWHQNLPQRDVLIRLNQRLGETCNLSIPDGDAMLYVD
HCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCCCCEEEEEE
RVETHWPLRIQLHVGSRVPLHATAAGKLCLSLINDGKLERYLKRAHLTAHTGQTITDPDR
ECCCCCCEEEEEEECCCCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHH
LREALRQIRQQGYSTDSEEFVPGMIAVAVPVLDRSGQLVATVSFHAPVQRLTLAEGLKHL
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHC
PDLRAAAEELTQLI
HHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8083169 [H]