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The map label for this gene is fumB [H]

Identifier: 119386824

GI number: 119386824

Start: 1271048

End: 1272553

Strand: Direct

Name: fumB [H]

Synonym: Pden_4119

Alternate gene names: 119386824

Gene position: 1271048-1272553 (Clockwise)

Preceding gene: 119386823

Following gene: 119386837

Centisome position: 73.47

GC content: 69.39

Gene sequence:

>1506_bases
ATGCCCGCGATCACCGAAGGCCATGTCATCGACAGCGTGGCCGACGCCCTGCAGTTCATATCGTATTACCATCCGCGCGA
TTTCATCCGGGCGATGTCGGCGGCATGGGCGGCCGAGCGAAGCCCGGCGGCCAGGAACGCCATGGCGCAGATCCTGGTCA
ATTCCCGCATGGCCGCGATGGGGCACCGGCCGATCTGCCAGGATACGGGCGTCGCCAACATCACCGCCCGGATCGGCGTG
CGGGCGCAACTGGACTGGGACCGGCCGCTGCAAGAGATCGTGGACGACGCCGTGCGCCGGGCGTATCGCAACGATGCCAA
TCCGCTTCGCGCCTCGGTCGTGGCCGATCCGTTCGGGCGGCGGCGCAACACCAGCGACAATGCCCCGGCCATGCTGCATG
TCGAGATGGTCGCTGGCACCTCGGTCGAGTTCGACGTCTCGGCCAAGGGCGGCGGTTCCGAGAACAAGACGAAATTCGCG
GTCCTGAACCCCTCGGCCGATGTCGCCGACTGGGTGGTCGGGACGGTCGAGGCGATGGGCGCGGGCTGGTGTCCTCCGGG
CATCCTGGGCCTTGGCATCGGCGGCAGCGTGGACAAGGCCATGGTCATGGCGAAACAGGCCCTGAACCAGCCGATCGATG
CGACCGAGCTGCGCACGCGTGGCCCCGCGACCCCCGAAGAGGAGCTGCGGCTGGAGATCATGGACCGGGTCAATGCGCTT
GGCATCGGCGCGCAGGGCCTGGGCGGCATCACGACGGTCCTGGACGTGAAGGTTTGCAGCTTTCCCACCCATGCCGCCTC
GCTGCCGGTGGCGCTGGTGCCGAACTGCGCGGCGACGCGCCATGTGCATTTCACGCTGGACGGATCCGGGCCGGCGCGGC
TGGTTCCGCCCGATTTGTCCGACTGGCCCGAACTCGACTTTCGCGACGCCTGGAAGGATGCCCGCCGCGTCGACCTGGAC
CGCCTGACCCGCGAGCAGATCGCAAGCTGGCGGCCGGGCGAGACCCTGCTGCTGTCCGGGCGCCTGCTGACCGGGCGCGA
CGCCGCCCATCGCCGGATCGAGCAGATGCTGGCCGAGGGCAAGCCCCTGCCGGTCAGCTTTCGCAACCGTGCGATCTATT
ATGTCGGCCCGGTCGATCCCGTCGGCGGTGAGGTGGTCGGGCCGGCCGGTCCCACCACCTCGACCCGGATGGACCGCTAC
AGCGACATGATGCTGGAAACGCTGGGCGTGTCGGTGATGATCGGCAAGGCCGAGCGCGGCCAGGAGACGATCGAGGCCAT
CGCCCGGAACGGCGCCGCCTATCTGGTCGCGGTGGGCGGCGCGGCCTATCTGGTCTCGCAGGCGATCCGCGAGGCGCGCG
TGCTGGGGTTCGAGGATCTTGGCATGGAGGCGATCCACGAATTCACCGTCAGGGACATGCCGGTCACGGTGGCGGTGGAC
GCGCGCGGCACCTCGGTCCATGCCAGCGGCCCGCGGCAATGGCGGATGCCGCCGCCCCGGGCGTGA

Upstream 100 bases:

>100_bases
GCGCGAGATCCTGCGCGACCTGGTCCGCAATCACCGCTGGATCGGTGCCACGGTCCTTGCCGCCGAGGAACCCGCCAAGC
CGCCCCCCTGAGGAACAGCC

Downstream 100 bases:

>100_bases
CGCGAAGGGACCGCTTTCGCAGCCCCTTCCTGTTCAGAACCGCAGTCAGAACCCAAGCAGGTTCGGCAACAGCAGGGTGA
TGGCCGGTATGTATGTGATC

Product: tartrate/fumarate subfamily Fe-S type hydro-lyase alpha subunit

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MPAITEGHVIDSVADALQFISYYHPRDFIRAMSAAWAAERSPAARNAMAQILVNSRMAAMGHRPICQDTGVANITARIGV
RAQLDWDRPLQEIVDDAVRRAYRNDANPLRASVVADPFGRRRNTSDNAPAMLHVEMVAGTSVEFDVSAKGGGSENKTKFA
VLNPSADVADWVVGTVEAMGAGWCPPGILGLGIGGSVDKAMVMAKQALNQPIDATELRTRGPATPEEELRLEIMDRVNAL
GIGAQGLGGITTVLDVKVCSFPTHAASLPVALVPNCAATRHVHFTLDGSGPARLVPPDLSDWPELDFRDAWKDARRVDLD
RLTREQIASWRPGETLLLSGRLLTGRDAAHRRIEQMLAEGKPLPVSFRNRAIYYVGPVDPVGGEVVGPAGPTTSTRMDRY
SDMMLETLGVSVMIGKAERGQETIEAIARNGAAYLVAVGGAAYLVSQAIREARVLGFEDLGMEAIHEFTVRDMPVTVAVD
ARGTSVHASGPRQWRMPPPRA

Sequences:

>Translated_501_residues
MPAITEGHVIDSVADALQFISYYHPRDFIRAMSAAWAAERSPAARNAMAQILVNSRMAAMGHRPICQDTGVANITARIGV
RAQLDWDRPLQEIVDDAVRRAYRNDANPLRASVVADPFGRRRNTSDNAPAMLHVEMVAGTSVEFDVSAKGGGSENKTKFA
VLNPSADVADWVVGTVEAMGAGWCPPGILGLGIGGSVDKAMVMAKQALNQPIDATELRTRGPATPEEELRLEIMDRVNAL
GIGAQGLGGITTVLDVKVCSFPTHAASLPVALVPNCAATRHVHFTLDGSGPARLVPPDLSDWPELDFRDAWKDARRVDLD
RLTREQIASWRPGETLLLSGRLLTGRDAAHRRIEQMLAEGKPLPVSFRNRAIYYVGPVDPVGGEVVGPAGPTTSTRMDRY
SDMMLETLGVSVMIGKAERGQETIEAIARNGAAYLVAVGGAAYLVSQAIREARVLGFEDLGMEAIHEFTVRDMPVTVAVD
ARGTSVHASGPRQWRMPPPRA
>Mature_500_residues
PAITEGHVIDSVADALQFISYYHPRDFIRAMSAAWAAERSPAARNAMAQILVNSRMAAMGHRPICQDTGVANITARIGVR
AQLDWDRPLQEIVDDAVRRAYRNDANPLRASVVADPFGRRRNTSDNAPAMLHVEMVAGTSVEFDVSAKGGGSENKTKFAV
LNPSADVADWVVGTVEAMGAGWCPPGILGLGIGGSVDKAMVMAKQALNQPIDATELRTRGPATPEEELRLEIMDRVNALG
IGAQGLGGITTVLDVKVCSFPTHAASLPVALVPNCAATRHVHFTLDGSGPARLVPPDLSDWPELDFRDAWKDARRVDLDR
LTREQIASWRPGETLLLSGRLLTGRDAAHRRIEQMLAEGKPLPVSFRNRAIYYVGPVDPVGGEVVGPAGPTTSTRMDRYS
DMMLETLGVSVMIGKAERGQETIEAIARNGAAYLVAVGGAAYLVSQAIREARVLGFEDLGMEAIHEFTVRDMPVTVAVDA
RGTSVHASGPRQWRMPPPRA

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=468, Percent_Identity=31.8376068376068, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI1787897, Length=468, Percent_Identity=31.6239316239316, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI1789442, Length=273, Percent_Identity=30.03663003663, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789443, Length=173, Percent_Identity=29.4797687861272, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 53942; Mature: 53811

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAITEGHVIDSVADALQFISYYHPRDFIRAMSAAWAAERSPAARNAMAQILVNSRMAAM
CCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GHRPICQDTGVANITARIGVRAQLDWDRPLQEIVDDAVRRAYRNDANPLRASVVADPFGR
CCCCCCCCCCCCEEEEEECEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCC
RRNTSDNAPAMLHVEMVAGTSVEFDVSAKGGGSENKTKFAVLNPSADVADWVVGTVEAMG
CCCCCCCCCEEEEEEEEECCCEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHC
AGWCPPGILGLGIGGSVDKAMVMAKQALNQPIDATELRTRGPATPEEELRLEIMDRVNAL
CCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHC
GIGAQGLGGITTVLDVKVCSFPTHAASLPVALVPNCAATRHVHFTLDGSGPARLVPPDLS
CCCCCCCCCHHHEEEEEEECCCCHHHCCCEEECCCCCCEEEEEEEECCCCCCEECCCCCC
DWPELDFRDAWKDARRVDLDRLTREQIASWRPGETLLLSGRLLTGRDAAHRRIEQMLAEG
CCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEECEEECCCHHHHHHHHHHHHCC
KPLPVSFRNRAIYYVGPVDPVGGEVVGPAGPTTSTRMDRYSDMMLETLGVSVMIGKAERG
CCCCEEECCCEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCC
QETIEAIARNGAAYLVAVGGAAYLVSQAIREARVLGFEDLGMEAIHEFTVRDMPVTVAVD
HHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHCCCCEEEEEE
ARGTSVHASGPRQWRMPPPRA
CCCCEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
PAITEGHVIDSVADALQFISYYHPRDFIRAMSAAWAAERSPAARNAMAQILVNSRMAAM
CCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GHRPICQDTGVANITARIGVRAQLDWDRPLQEIVDDAVRRAYRNDANPLRASVVADPFGR
CCCCCCCCCCCCEEEEEECEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCC
RRNTSDNAPAMLHVEMVAGTSVEFDVSAKGGGSENKTKFAVLNPSADVADWVVGTVEAMG
CCCCCCCCCEEEEEEEEECCCEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHC
AGWCPPGILGLGIGGSVDKAMVMAKQALNQPIDATELRTRGPATPEEELRLEIMDRVNAL
CCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHC
GIGAQGLGGITTVLDVKVCSFPTHAASLPVALVPNCAATRHVHFTLDGSGPARLVPPDLS
CCCCCCCCCHHHEEEEEEECCCCHHHCCCEEECCCCCCEEEEEEEECCCCCCEECCCCCC
DWPELDFRDAWKDARRVDLDRLTREQIASWRPGETLLLSGRLLTGRDAAHRRIEQMLAEG
CCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEECEEECCCHHHHHHHHHHHHCC
KPLPVSFRNRAIYYVGPVDPVGGEVVGPAGPTTSTRMDRYSDMMLETLGVSVMIGKAERG
CCCCEEECCCEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCC
QETIEAIARNGAAYLVAVGGAAYLVSQAIREARVLGFEDLGMEAIHEFTVRDMPVTVAVD
HHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHCCCCEEEEEE
ARGTSVHASGPRQWRMPPPRA
CCCCEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]