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The map label for this gene is eno

Identifier: 119386767

GI number: 119386767

Start: 1213306

End: 1214583

Strand: Direct

Name: eno

Synonym: Pden_4060

Alternate gene names: 119386767

Gene position: 1213306-1214583 (Clockwise)

Preceding gene: 119386766

Following gene: 119386771

Centisome position: 70.13

GC content: 67.29

Gene sequence:

>1278_bases
ATGACGGCCATCATCGACATATTCGCGCGCGAGATACTGGACAGCCGGGGCAACCCGACGGTCGAGGTCGATGTGACGCT
TGAGGACGGCACCATGGGCCGGGCGGCGGTGCCCTCGGGGGCGTCCACCGGGGCGCATGAGGCGGTCGAGAAGCGCGACG
GCGACAAGGCCCGCTATCTGGGCAAGGGTGTGCTGGAGGCCGTCGCCGCCGTCAACGGCGAGATCGCCGAGAACCTGATC
GGCGAGGACGCGACCGAGCAGGTCGCCATCGACCGCATGATGATCGAACTGGACGGCACCCCGAACAAGGGCCGGCTGGG
CGCCAACGCCATCCTGGGCGTGTCGCTGGCGGTGGCCAAGGCCGCGGCCGAGGCCTGCTCGCAGCCGCTCTACCGCTATG
TCGGCGGCGCGGGCGCCCGCGTGCTGCCGGTGCCGATGATGAACATCATCAACGGCGGCGAACATGCCGACAACCCGATC
GACATCCAGGAATTCATGATCATGCCGGTGGCGGCGGAGAACATCCGCGAAGCCGTGCGCATGGGCTCGGAAGTGTTCCA
CACGCTGAAGAAGGAGCTGTCCTCGGCGGGTCTGGCGACGGGCGTCGGCGACGAGGGCGGCTTCGCCCCGAACCTCAGCA
GCACCCGCGATGCGCTGGACTTCATCCTCAAGGCCATCGAGAAGGCCGGCTACCAGCCCGGCGACGACATCATGCTGGCG
CTGGACTGCGCCTCGACCGAATATTTCAAGGGCGGCAAATACGAGATGGCCGGCGAGGGCAAGTCGCTGAGCCCGGCCGA
GAACGTCGCCTACCTGGAAGCACTGTGCAACGACTACCCGATCCTGTCGATCGAGGACGGCTGCGCCGAGGACGACTGGG
ACGGCTGGAAACTGCTGACCGACACGCTGGGCGGCCGCGTACAACTGGTCGGCGACGACCTGTTCGTGACCAACCCGGCA
CGTCTGGCCGAGGGCATCGCCAAGGGCTGCGGCAACTCGCTCCTGGTCAAGGTGAACCAGATCGGCACCCTGACCGAGAC
GCTGGACGCGGTGCGCATGGCCGACCGCGCACGCTATACCTCGGTGATGTCGCACCGCTCGGGCGAGACCGAGGACGCCA
CCATCGCCGACCTGGCCGTGGCGACCAATTGCGGCCAGATCAAGACCGGCAGCCTCGCGCGCTCGGACCGGCTGGCGAAA
TACAACCAGCTCATCCGCATCGAGGAAATGCTCGGCGCCACCGCCGAATATGCCGGGAAATCCATCCTGCGCGGCTGA

Upstream 100 bases:

>100_bases
TCCCGGCGAGGACGGGGTGGCCGGGGCAGAATGACGCTTTTCCGCAACTGCGAAGGCGGTTAAACAGCGCGAAAGCTCGT
CAGCCAAGGGGGACGCAGAC

Downstream 100 bases:

>100_bases
ACCGGACGAGATCGGAACGCTGAAGGGCGGCCCCGGTGGCCGCCCTTTTCATATCCACCAGCGATGCGTCAGGGCAAAAG
AGCCAAGCGCAAGCCGGCGG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTAIIDIFAREILDSRGNPTVEVDVTLEDGTMGRAAVPSGASTGAHEAVEKRDGDKARYLGKGVLEAVAAVNGEIAENLI
GEDATEQVAIDRMMIELDGTPNKGRLGANAILGVSLAVAKAAAEACSQPLYRYVGGAGARVLPVPMMNIINGGEHADNPI
DIQEFMIMPVAAENIREAVRMGSEVFHTLKKELSSAGLATGVGDEGGFAPNLSSTRDALDFILKAIEKAGYQPGDDIMLA
LDCASTEYFKGGKYEMAGEGKSLSPAENVAYLEALCNDYPILSIEDGCAEDDWDGWKLLTDTLGGRVQLVGDDLFVTNPA
RLAEGIAKGCGNSLLVKVNQIGTLTETLDAVRMADRARYTSVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAK
YNQLIRIEEMLGATAEYAGKSILRG

Sequences:

>Translated_425_residues
MTAIIDIFAREILDSRGNPTVEVDVTLEDGTMGRAAVPSGASTGAHEAVEKRDGDKARYLGKGVLEAVAAVNGEIAENLI
GEDATEQVAIDRMMIELDGTPNKGRLGANAILGVSLAVAKAAAEACSQPLYRYVGGAGARVLPVPMMNIINGGEHADNPI
DIQEFMIMPVAAENIREAVRMGSEVFHTLKKELSSAGLATGVGDEGGFAPNLSSTRDALDFILKAIEKAGYQPGDDIMLA
LDCASTEYFKGGKYEMAGEGKSLSPAENVAYLEALCNDYPILSIEDGCAEDDWDGWKLLTDTLGGRVQLVGDDLFVTNPA
RLAEGIAKGCGNSLLVKVNQIGTLTETLDAVRMADRARYTSVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAK
YNQLIRIEEMLGATAEYAGKSILRG
>Mature_424_residues
TAIIDIFAREILDSRGNPTVEVDVTLEDGTMGRAAVPSGASTGAHEAVEKRDGDKARYLGKGVLEAVAAVNGEIAENLIG
EDATEQVAIDRMMIELDGTPNKGRLGANAILGVSLAVAKAAAEACSQPLYRYVGGAGARVLPVPMMNIINGGEHADNPID
IQEFMIMPVAAENIREAVRMGSEVFHTLKKELSSAGLATGVGDEGGFAPNLSSTRDALDFILKAIEKAGYQPGDDIMLAL
DCASTEYFKGGKYEMAGEGKSLSPAENVAYLEALCNDYPILSIEDGCAEDDWDGWKLLTDTLGGRVQLVGDDLFVTNPAR
LAEGIAKGCGNSLLVKVNQIGTLTETLDAVRMADRARYTSVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAKY
NQLIRIEEMLGATAEYAGKSILRG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI4503571, Length=432, Percent_Identity=50.6944444444444, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI5803011, Length=433, Percent_Identity=51.0392609699769, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI301897477, Length=434, Percent_Identity=50, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI301897469, Length=434, Percent_Identity=50, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI301897479, Length=432, Percent_Identity=46.0648148148148, Blast_Score=352, Evalue=4e-97,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=26.7857142857143, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=26.7857142857143, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=26.7857142857143, Blast_Score=99, Evalue=8e-21,
Organism=Escherichia coli, GI1789141, Length=430, Percent_Identity=62.5581395348837, Blast_Score=528, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=53.9351851851852, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=53.9351851851852, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI32563855, Length=192, Percent_Identity=46.875, Blast_Score=174, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6323985, Length=439, Percent_Identity=50.5694760820046, Blast_Score=399, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324974, Length=439, Percent_Identity=50.1138952164009, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324969, Length=439, Percent_Identity=50.1138952164009, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6321693, Length=438, Percent_Identity=49.3150684931507, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321968, Length=438, Percent_Identity=49.0867579908676, Blast_Score=376, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580918, Length=431, Percent_Identity=51.508120649652, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580916, Length=431, Percent_Identity=51.508120649652, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580920, Length=431, Percent_Identity=51.508120649652, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580914, Length=431, Percent_Identity=51.508120649652, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI281360527, Length=431, Percent_Identity=51.508120649652, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI17137654, Length=431, Percent_Identity=51.508120649652, Blast_Score=398, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_PARDP (A1B9D2)

Other databases:

- EMBL:   CP000490
- RefSeq:   YP_917822.1
- ProteinModelPortal:   A1B9D2
- SMR:   A1B9D2
- STRING:   A1B9D2
- GeneID:   4582611
- GenomeReviews:   CP000490_GR
- KEGG:   pde:Pden_4060
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   A1B9D2
- ProtClustDB:   PRK00077
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 44937; Mature: 44806

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIIDIFAREILDSRGNPTVEVDVTLEDGTMGRAAVPSGASTGAHEAVEKRDGDKARYL
CHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHH
GKGVLEAVAAVNGEIAENLIGEDATEQVAIDRMMIELDGTPNKGRLGANAILGVSLAVAK
HHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHH
AAAEACSQPLYRYVGGAGARVLPVPMMNIINGGEHADNPIDIQEFMIMPVAAENIREAVR
HHHHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
MGSEVFHTLKKELSSAGLATGVGDEGGFAPNLSSTRDALDFILKAIEKAGYQPGDDIMLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEE
LDCASTEYFKGGKYEMAGEGKSLSPAENVAYLEALCNDYPILSIEDGCAEDDWDGWKLLT
EEECCCHHHCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHH
DTLGGRVQLVGDDLFVTNPARLAEGIAKGCGNSLLVKVNQIGTLTETLDAVRMADRARYT
HHCCCEEEEEECCEEEECHHHHHHHHHHHCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHH
SVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAKYNQLIRIEEMLGATAEYAGK
HHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
SILRG
HHCCC
>Mature Secondary Structure 
TAIIDIFAREILDSRGNPTVEVDVTLEDGTMGRAAVPSGASTGAHEAVEKRDGDKARYL
HHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHH
GKGVLEAVAAVNGEIAENLIGEDATEQVAIDRMMIELDGTPNKGRLGANAILGVSLAVAK
HHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHH
AAAEACSQPLYRYVGGAGARVLPVPMMNIINGGEHADNPIDIQEFMIMPVAAENIREAVR
HHHHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
MGSEVFHTLKKELSSAGLATGVGDEGGFAPNLSSTRDALDFILKAIEKAGYQPGDDIMLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEE
LDCASTEYFKGGKYEMAGEGKSLSPAENVAYLEALCNDYPILSIEDGCAEDDWDGWKLLT
EEECCCHHHCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHH
DTLGGRVQLVGDDLFVTNPARLAEGIAKGCGNSLLVKVNQIGTLTETLDAVRMADRARYT
HHCCCEEEEEECCEEEECHHHHHHHHHHHCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHH
SVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAKYNQLIRIEEMLGATAEYAGK
HHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
SILRG
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA