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The map label for this gene is rlmE

Identifier: 119386697

GI number: 119386697

Start: 1129363

End: 1130133

Strand: Direct

Name: rlmE

Synonym: Pden_3990

Alternate gene names: 119386697

Gene position: 1129363-1130133 (Clockwise)

Preceding gene: 119386696

Following gene: 119386700

Centisome position: 65.28

GC content: 69.0

Gene sequence:

>771_bases
ATGAGCGAAGGCAAGAAGCCGGGCTCCGGGCGCAAGAGCTCGGGCCGCGGCCAGCGCGACCTGCGGGTGCGGGTCAAATC
GGCCAAGGGACGCAAGCTTTCCAGCACGCTCTGGCTGGAGCGGCAGTTGAACGACCCCTATGTCGCGCGGGCGAAGCGCG
AGGGCTATCGCGGCCGCGCCGCCTACAAGATCCTGGAACTGGACGACAAGTATCGCTTCCTGGTGCCGGGGGCGCGGGTG
GTCGATCTGGGCTGCGCGCCGGGCGGCTGGTGCCAGGTGGCGGTCGCGCGGGTGAACGCCCTGGGCGACAAGTCCGGCAA
GAAGGTCGGCCGCGTGCTGGGCGTGGACCTGCAAGAGGTCGATCCGATCCCGGGGGCCGAGATCCATCAGCTGGACTTCC
TGTCGGAGGGTGCGGACGAACAGGTCAAGGCATGGCTGGGCGGCCGGGCCGATGTGGTGATGTCGGACATGGCCGCGGCC
TCGTCCGGGCACAAGGGCACCGACCACCTGCGCATCGTCGCGCTGGTCGAGGCGGCGGCGCAACTGGCCTTCGACGTGCT
GGAGCCGGGCGGCACCTTCGTCGCCAAGGTTCTGGCCGGCGGGGCCGAGAACGAGATGCAGGCCATGCTCAAGCGCAATT
TCCGCAAGGTGGCGAATGTGAAGCCCCCGGCAAGCCGCTCCGACTCTTCCGAGAAATTCGTGGTGGCCCAAGGGTTCCGC
GGCCGTGGCGACGCGGAGGATGTCGAAAACGGCGAGGACGACGGGGCATGA

Upstream 100 bases:

>100_bases
CTGTCGGTCGCCGATCGCGGCCTGCGCGAGGGGTTGCTTTATGCGCAGATGGTCAAGGACGGGGTGCTGGCCCCGGATGG
AATGAACGGGGTGGCATGAG

Downstream 100 bases:

>100_bases
GGATCGGGAAGGCGCCGGGCCGGGCGGCCCGCTGCCGTTTCCGCCCGCAGAGCCCCCGATAGCGGCCTGCGCCGTGGCGC
ATGGCGGGATGCGGCTTTGG

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSEGKKPGSGRKSSGRGQRDLRVRVKSAKGRKLSSTLWLERQLNDPYVARAKREGYRGRAAYKILELDDKYRFLVPGARV
VDLGCAPGGWCQVAVARVNALGDKSGKKVGRVLGVDLQEVDPIPGAEIHQLDFLSEGADEQVKAWLGGRADVVMSDMAAA
SSGHKGTDHLRIVALVEAAAQLAFDVLEPGGTFVAKVLAGGAENEMQAMLKRNFRKVANVKPPASRSDSSEKFVVAQGFR
GRGDAEDVENGEDDGA

Sequences:

>Translated_256_residues
MSEGKKPGSGRKSSGRGQRDLRVRVKSAKGRKLSSTLWLERQLNDPYVARAKREGYRGRAAYKILELDDKYRFLVPGARV
VDLGCAPGGWCQVAVARVNALGDKSGKKVGRVLGVDLQEVDPIPGAEIHQLDFLSEGADEQVKAWLGGRADVVMSDMAAA
SSGHKGTDHLRIVALVEAAAQLAFDVLEPGGTFVAKVLAGGAENEMQAMLKRNFRKVANVKPPASRSDSSEKFVVAQGFR
GRGDAEDVENGEDDGA
>Mature_255_residues
SEGKKPGSGRKSSGRGQRDLRVRVKSAKGRKLSSTLWLERQLNDPYVARAKREGYRGRAAYKILELDDKYRFLVPGARVV
DLGCAPGGWCQVAVARVNALGDKSGKKVGRVLGVDLQEVDPIPGAEIHQLDFLSEGADEQVKAWLGGRADVVMSDMAAAS
SGHKGTDHLRIVALVEAAAQLAFDVLEPGGTFVAKVLAGGAENEMQAMLKRNFRKVANVKPPASRSDSSEKFVVAQGFRG
RGDAEDVENGEDDGA

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=217, Percent_Identity=41.9354838709677, Blast_Score=174, Evalue=6e-44,
Organism=Homo sapiens, GI29029591, Length=196, Percent_Identity=36.2244897959184, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI29029589, Length=196, Percent_Identity=36.2244897959184, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI7110661, Length=196, Percent_Identity=36.2244897959184, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI194097365, Length=206, Percent_Identity=34.9514563106796, Blast_Score=109, Evalue=2e-24,
Organism=Escherichia coli, GI1789569, Length=213, Percent_Identity=39.4366197183099, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17553474, Length=210, Percent_Identity=35.7142857142857, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17554650, Length=200, Percent_Identity=32.5, Blast_Score=110, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17553860, Length=206, Percent_Identity=33.0097087378641, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71987561, Length=206, Percent_Identity=33.0097087378641, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71987550, Length=206, Percent_Identity=33.0097087378641, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319535, Length=198, Percent_Identity=36.3636363636364, Blast_Score=116, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6319796, Length=201, Percent_Identity=34.3283582089552, Blast_Score=101, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6321302, Length=117, Percent_Identity=35.042735042735, Blast_Score=79, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21356387, Length=222, Percent_Identity=40.5405405405405, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24648639, Length=196, Percent_Identity=34.6938775510204, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=36.3636363636364, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI18859957, Length=197, Percent_Identity=36.0406091370558, Blast_Score=101, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_PARDP (A1B962)

Other databases:

- EMBL:   CP000490
- RefSeq:   YP_917752.1
- ProteinModelPortal:   A1B962
- SMR:   A1B962
- STRING:   A1B962
- GeneID:   4582541
- GenomeReviews:   CP000490_GR
- KEGG:   pde:Pden_3990
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- PhylomeDB:   A1B962
- ProtClustDB:   CLSK933653
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 27444; Mature: 27313

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: ACT_SITE 196-196 BINDING 88-88 BINDING 90-90 BINDING 116-116 BINDING 132-132 BINDING 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEGKKPGSGRKSSGRGQRDLRVRVKSAKGRKLSSTLWLERQLNDPYVARAKREGYRGRA
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCC
AYKILELDDKYRFLVPGARVVDLGCAPGGWCQVAVARVNALGDKSGKKVGRVLGVDLQEV
EEEEEEECCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHC
DPIPGAEIHQLDFLSEGADEQVKAWLGGRADVVMSDMAAASSGHKGTDHLRIVALVEAAA
CCCCCCCHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
QLAFDVLEPGGTFVAKVLAGGAENEMQAMLKRNFRKVANVKPPASRSDSSEKFVVAQGFR
HHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCC
GRGDAEDVENGEDDGA
CCCCCHHCCCCCCCCC
>Mature Secondary Structure 
SEGKKPGSGRKSSGRGQRDLRVRVKSAKGRKLSSTLWLERQLNDPYVARAKREGYRGRA
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCC
AYKILELDDKYRFLVPGARVVDLGCAPGGWCQVAVARVNALGDKSGKKVGRVLGVDLQEV
EEEEEEECCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHC
DPIPGAEIHQLDFLSEGADEQVKAWLGGRADVVMSDMAAASSGHKGTDHLRIVALVEAAA
CCCCCCCHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
QLAFDVLEPGGTFVAKVLAGGAENEMQAMLKRNFRKVANVKPPASRSDSSEKFVVAQGFR
HHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCC
GRGDAEDVENGEDDGA
CCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA