Click here to switch to the map view.

The map label for this gene is trmD [H]

Identifier: 119386590

GI number: 119386590

Start: 1016405

End: 1017274

Strand: Direct

Name: trmD [H]

Synonym: Pden_3883

Alternate gene names: 119386590

Gene position: 1016405-1017274 (Clockwise)

Preceding gene: 119386589

Following gene: 119386591

Centisome position: 58.75

GC content: 66.44

Gene sequence:

>870_bases
ATGAGCGAGGCTCCGAAATCCCACGGCCGGCTGTCCATCCGCGCCAGCACGCAGCCGCGCGACCTCATGGCCGAGCCGCA
GGTCAAGGGCGCCTGGACCGCACAGGTCGTCACGCTTTTCCCCGAGGCTTTTCCGGGGGTTCTGGGCCTTTCCTTGACGG
GAAAGGCGCTGGCCGAGGGGCTGTGGAACCTGCGCAGCATCGACCTGCGCCCCTTTGGCATCGGCAAGCATCGCAACGTG
GACGATACGCCCGCCGGCGGCGGCGCCGGCATGGTGATCCGTCCCGACGTGATGGATGCGGCGCTGCGCGAGGCGGGCGG
CGATCTGCCGGTGATCTATCTTTCGCCGCGCGGCAAGCCTTTCACCCAAGGCCGGGCCAGGGAGTTGGCGCGGGGCCCCG
GCATGACGCTGATCTGCGGCCGGTTCGAGGGCGTGGACCAGCGCGTGCTGGACGCCCATGGCGTCGAAGAGATCAGCATC
GGCGATTATGTCCTGACCGGCGGGGAACTGGCGGCTCAGGTCTTGATCGACGCGACGGTTCGCCTTATACCGCGCGTGCT
GGGGAATCAGGACTCTCTGGCCGAGGAATCCTTTTCCCCGGACCATGGCGGCCTGCTGGAACATCCGCAGTATACGAAGC
CCGCCCTTTGGGAAGGCCGCGCGATCCCGGAAGTGCTTTTGTCCGGCAATCACGCGGCTATTGCCAGTTGGCGGCGCGAC
ATGGCCGAAAGGCTGACCAAGGAACGCCGCCCCGACCTGTGGCGGGCATATGGGCAAGCCCATATGGACCCGGCAAAGGA
CCGACAGCTCTCGGGCGCATCAGACCAATCGCGGGACTACCGCGAGCATCAAGAGGAACCAAAGCGATGA

Upstream 100 bases:

>100_bases
GTCGCCGGCAGGTGCTGCTCTTGCCCTTTACCCGCGCCTTCGTGCCCACGGTCGATCTGGCCGCCGGCCGCATCGTCGCC
GACCCGCCGGAAGAGCAGGA

Downstream 100 bases:

>100_bases
ACCTGATCGCCCAGTTGGAAGCCGAGCAGATCGCCGCGCTCGGCAAGGACATCCCCGATTTCAAGGCCGGCGACACCGTG
CGCGTCGGCTACAAGGTGAC

Product: tRNA (guanine-N1)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSEAPKSHGRLSIRASTQPRDLMAEPQVKGAWTAQVVTLFPEAFPGVLGLSLTGKALAEGLWNLRSIDLRPFGIGKHRNV
DDTPAGGGAGMVIRPDVMDAALREAGGDLPVIYLSPRGKPFTQGRARELARGPGMTLICGRFEGVDQRVLDAHGVEEISI
GDYVLTGGELAAQVLIDATVRLIPRVLGNQDSLAEESFSPDHGGLLEHPQYTKPALWEGRAIPEVLLSGNHAAIASWRRD
MAERLTKERRPDLWRAYGQAHMDPAKDRQLSGASDQSRDYREHQEEPKR

Sequences:

>Translated_289_residues
MSEAPKSHGRLSIRASTQPRDLMAEPQVKGAWTAQVVTLFPEAFPGVLGLSLTGKALAEGLWNLRSIDLRPFGIGKHRNV
DDTPAGGGAGMVIRPDVMDAALREAGGDLPVIYLSPRGKPFTQGRARELARGPGMTLICGRFEGVDQRVLDAHGVEEISI
GDYVLTGGELAAQVLIDATVRLIPRVLGNQDSLAEESFSPDHGGLLEHPQYTKPALWEGRAIPEVLLSGNHAAIASWRRD
MAERLTKERRPDLWRAYGQAHMDPAKDRQLSGASDQSRDYREHQEEPKR
>Mature_288_residues
SEAPKSHGRLSIRASTQPRDLMAEPQVKGAWTAQVVTLFPEAFPGVLGLSLTGKALAEGLWNLRSIDLRPFGIGKHRNVD
DTPAGGGAGMVIRPDVMDAALREAGGDLPVIYLSPRGKPFTQGRARELARGPGMTLICGRFEGVDQRVLDAHGVEEISIG
DYVLTGGELAAQVLIDATVRLIPRVLGNQDSLAEESFSPDHGGLLEHPQYTKPALWEGRAIPEVLLSGNHAAIASWRRDM
AERLTKERRPDLWRAYGQAHMDPAKDRQLSGASDQSRDYREHQEEPKR

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=222, Percent_Identity=46.3963963963964, Blast_Score=178, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 31588; Mature: 31457

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAPKSHGRLSIRASTQPRDLMAEPQVKGAWTAQVVTLFPEAFPGVLGLSLTGKALAEG
CCCCCCCCCEEEEEECCCCHHHCCCCCCCCCHHHHHHHHHHHHHCHHEECCCCHHHHHHH
LWNLRSIDLRPFGIGKHRNVDDTPAGGGAGMVIRPDVMDAALREAGGDLPVIYLSPRGKP
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHCCCCCEEEEECCCCCC
FTQGRARELARGPGMTLICGRFEGVDQRVLDAHGVEEISIGDYVLTGGELAAQVLIDATV
CCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCEEECCCEEEECHHHHHHHHHHHHH
RLIPRVLGNQDSLAEESFSPDHGGLLEHPQYTKPALWEGRAIPEVLLSGNHAAIASWRRD
HHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHH
MAERLTKERRPDLWRAYGQAHMDPAKDRQLSGASDQSRDYREHQEEPKR
HHHHHHHHCCCHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
SEAPKSHGRLSIRASTQPRDLMAEPQVKGAWTAQVVTLFPEAFPGVLGLSLTGKALAEG
CCCCCCCCEEEEEECCCCHHHCCCCCCCCCHHHHHHHHHHHHHCHHEECCCCHHHHHHH
LWNLRSIDLRPFGIGKHRNVDDTPAGGGAGMVIRPDVMDAALREAGGDLPVIYLSPRGKP
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHCCCCCEEEEECCCCCC
FTQGRARELARGPGMTLICGRFEGVDQRVLDAHGVEEISIGDYVLTGGELAAQVLIDATV
CCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCEEECCCEEEECHHHHHHHHHHHHH
RLIPRVLGNQDSLAEESFSPDHGGLLEHPQYTKPALWEGRAIPEVLLSGNHAAIASWRRD
HHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHH
MAERLTKERRPDLWRAYGQAHMDPAKDRQLSGASDQSRDYREHQEEPKR
HHHHHHHHCCCHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA