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The map label for this gene is kefA [C]

Identifier: 119386569

GI number: 119386569

Start: 994597

End: 997089

Strand: Direct

Name: kefA [C]

Synonym: Pden_3862

Alternate gene names: 119386569

Gene position: 994597-997089 (Clockwise)

Preceding gene: 119386568

Following gene: 119386570

Centisome position: 57.49

GC content: 68.99

Gene sequence:

>2493_bases
ATGCCCCGCCTCGCCGCGCTGTCCCGGCTCGCATCGCTGATCCTGGTGCTGTTTCTGGCGCTGAACCTGCCTGCCCTTGC
CCAGCAAGAGCAGGAGGCGCCGGATCTTGCCGCCTGGGACAAGCTGGCCGAGCAGGCCGAGCAGATCGTGGCATCGGGCT
CGGCCAACGATGTCCGCTTGCAGGCCATCCGCGAAGAGGTGGTGAAATGGCGCGAGCGGCTGAAGGCGGGGCAGGGTATC
AACGCCACCCGCATCGCCACGCTCAAGGACCAGATCGCCGCGCTGGGGACACCCCCTGCCGAGGGCCAGACCGAGCCCGA
GGAGATCGCCGCCCGCCGCAAGGAACTGAACGAGCAGCTTGCCACGTTGCAGGCACCGGGCTTGCAGGCGGTCGAAGCCT
ATGGCCGCGCCGACGGCATCGTCGCCCAGATCGACCAGACCCTGCGCGCCCGCCAGACCTTTGCGCTGATCCGCAAGACG
CCCACGCCGCTGAACCCGGCGAACTGGGGACCGGCCATGGCCGAAGCGGGCCATGTCGCCGGACAGATCTATGCCGAGGC
GCGGGCGCGCTGGGACGGCACCGGCGGCATGTCCGGCTTTACCCAGCGCCTGCCGCTGATCGGGGGTTTCCTGCTGGTGG
CGCTGATCCTGCTGTCGCGGGGCCGGCACTGGATCGATTCGCTGCCCTCTCGGCTGTCGGCGCGGGCCAGCGAGCGGTCG
CGCGCGGCGCTGATCTTCGGCGTCTCGCTGGGCCAGATCGCCATCCCGCTGATCGGCGTGATCCTGTTCGCGGCGGCGCT
GGCCTCGACCGACCTGCTCGACGACTGGGGCCTGCCGCTTCTGCGCTCGGTGCCGGCGGCGGGGCTGTCCTTCTTTGGCG
GCATCTGGCTTGGGCGGCAGCTGTTCCCGGCCGCCGATACCGCCGTCCAGCCGCCCCTGCCGATGGACGAGGAGCGCCGT
GCCAAGGCACGTTTCCGCGTCACGCTGCTGGCTTCGGCGTTGGCGCTGCACCAGCTTTTCGCCCGCTCGATCCTGCCGCT
TTCCGGCTTCAACAGCCAGAACGACAGCGGCACCGTGCCCCAGCGCCTCAGCGAGGCTTCGGCCGGGGTCTGGCACTTCC
TGCTGATCCTGTTCGGGGCCTTCTGCCTGTTCAGGCTGTGCAACCTGCTGCGCGGGCTGCGCCCCGCCGAAGCGGCCGAT
ACGCCCGATTACCGCGTCCGCGTGGTAAGCTTTCTGGCCATGACGGGGCGGCTGGCGGCGCTTGTCGCGCCGGTGCTGGT
GGCGGTGGGCTATGTCACGGCGGGCAATGCGCTGCTCTGGGCGCTGGTGCTGACGCTGGCGCTGGTCGGGCTGCTGGTCA
TCCTGCAGGATTTCATCGCCGATCTTTATGCCCTGGCCAAGGGCGGCGACCAGTCGGCGCGGGATGCGCTGATGCCGGTG
CTGATCGGATTCGCGCTGATCCTGCTGTCGCTGCCGCTTTTCGCGCTGATCTGGGGCGCGCGCGCCAGCGACCTGGCCGA
GGCCTGGACCAAGGCGCAGCAGGGCTTTTCCTTCGGCGGCGTGCGGCTGTCGCCCATGGCGATGCTGACTTTCCTGATCG
TCTTTGCCGCCGGCTATTTCCTGACCAATTTCGTGCAGGGGGCATTCCGCAGCTCGATCCTGCCCAAGACGAAACTGGAC
TCGGGCGGGCAGAACGCGGTCGCCTCGATGATCGGTTATGTCGGGCTGGCGCTGGCGGCGGTCTTTGCCGTCACCTCGGC
CGGGATCGACCTGACCTCGCTGGCCTTCGTCGCCGGTGCGCTTTCCGTCGGCATCGGTTTCGGCATGCAGCAGGTGGTGT
CGAACTTCGTCTCGGGCATCATCCTGCTGGTCGAGCGTCCCATCGCCGTCGGCGATTGGATCGAGGTCGGCGGCCAGCAG
GGCGTCGTCAAGAAGATGGCGGTGCGCGCCACGCAGATCCAGACCTTCGACCGCACCCAGGTGATCGTGCCGAACTCGAA
CCTGATCACCCAGCCGGTCACGAACTGGACGCGCGGCAGCCTGTCGGGCCGGATCATCGTGCCGGTCACCGTCTCGCATG
GTTCGGACAGCCGCAAGGTCGCCGAGCTGCTGCGCGAGATCGCCGAGGATCAGCCCACGGTGCTGATCAACCCGGCGCCC
GCCGTGCTGTTGCGCAGCATCACCGCCGACGGGCTGAACTTCGAACTGCGCGCCGTGCTGTCGGACATCAACGGCGGGTC
CGGGGTTTCGTCCGAGATCAACCACCAGATCCTCGAACGCTTTGCGGCGGCCGGCATCGTCCTGCCGGGCAGCACCCGCC
CCTCGCGCGACATCTACCTGCATGGCGAGGCCGACATGCCCGCGCCGGCGCCGAAACCCGTGCCCGCCGCGGCAGCTGAT
CCGCGCAAGACCACGCCGCAGGAGGCCGAGACGCATGACAGTGGCGAATTCGGTCCCGCCGGCATGCAGGACGACGATGG
AGGCCGCGCATGA

Upstream 100 bases:

>100_bases
TCAACCCCGACTTCCTGCGGGCCAAGGGCCTGCCGGTGCCGGAATGGATGACGCGCAAGCCGGCGGACCTGCCCGAGGTT
TACGAGGACTGATCCGAACC

Downstream 100 bases:

>100_bases
CTTCACCCGAGGAGCCGCGCTTCCTGGCTGCCATCCCCGTCCTGCGCAGCTTCGACGAGGGCAAGGCGCGGGAATTCTAC
TGCGGCTTCCTGGGCTTCTC

Product: MscS mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 830; Mature: 829

Protein sequence:

>830_residues
MPRLAALSRLASLILVLFLALNLPALAQQEQEAPDLAAWDKLAEQAEQIVASGSANDVRLQAIREEVVKWRERLKAGQGI
NATRIATLKDQIAALGTPPAEGQTEPEEIAARRKELNEQLATLQAPGLQAVEAYGRADGIVAQIDQTLRARQTFALIRKT
PTPLNPANWGPAMAEAGHVAGQIYAEARARWDGTGGMSGFTQRLPLIGGFLLVALILLSRGRHWIDSLPSRLSARASERS
RAALIFGVSLGQIAIPLIGVILFAAALASTDLLDDWGLPLLRSVPAAGLSFFGGIWLGRQLFPAADTAVQPPLPMDEERR
AKARFRVTLLASALALHQLFARSILPLSGFNSQNDSGTVPQRLSEASAGVWHFLLILFGAFCLFRLCNLLRGLRPAEAAD
TPDYRVRVVSFLAMTGRLAALVAPVLVAVGYVTAGNALLWALVLTLALVGLLVILQDFIADLYALAKGGDQSARDALMPV
LIGFALILLSLPLFALIWGARASDLAEAWTKAQQGFSFGGVRLSPMAMLTFLIVFAAGYFLTNFVQGAFRSSILPKTKLD
SGGQNAVASMIGYVGLALAAVFAVTSAGIDLTSLAFVAGALSVGIGFGMQQVVSNFVSGIILLVERPIAVGDWIEVGGQQ
GVVKKMAVRATQIQTFDRTQVIVPNSNLITQPVTNWTRGSLSGRIIVPVTVSHGSDSRKVAELLREIAEDQPTVLINPAP
AVLLRSITADGLNFELRAVLSDINGGSGVSSEINHQILERFAAAGIVLPGSTRPSRDIYLHGEADMPAPAPKPVPAAAAD
PRKTTPQEAETHDSGEFGPAGMQDDDGGRA

Sequences:

>Translated_830_residues
MPRLAALSRLASLILVLFLALNLPALAQQEQEAPDLAAWDKLAEQAEQIVASGSANDVRLQAIREEVVKWRERLKAGQGI
NATRIATLKDQIAALGTPPAEGQTEPEEIAARRKELNEQLATLQAPGLQAVEAYGRADGIVAQIDQTLRARQTFALIRKT
PTPLNPANWGPAMAEAGHVAGQIYAEARARWDGTGGMSGFTQRLPLIGGFLLVALILLSRGRHWIDSLPSRLSARASERS
RAALIFGVSLGQIAIPLIGVILFAAALASTDLLDDWGLPLLRSVPAAGLSFFGGIWLGRQLFPAADTAVQPPLPMDEERR
AKARFRVTLLASALALHQLFARSILPLSGFNSQNDSGTVPQRLSEASAGVWHFLLILFGAFCLFRLCNLLRGLRPAEAAD
TPDYRVRVVSFLAMTGRLAALVAPVLVAVGYVTAGNALLWALVLTLALVGLLVILQDFIADLYALAKGGDQSARDALMPV
LIGFALILLSLPLFALIWGARASDLAEAWTKAQQGFSFGGVRLSPMAMLTFLIVFAAGYFLTNFVQGAFRSSILPKTKLD
SGGQNAVASMIGYVGLALAAVFAVTSAGIDLTSLAFVAGALSVGIGFGMQQVVSNFVSGIILLVERPIAVGDWIEVGGQQ
GVVKKMAVRATQIQTFDRTQVIVPNSNLITQPVTNWTRGSLSGRIIVPVTVSHGSDSRKVAELLREIAEDQPTVLINPAP
AVLLRSITADGLNFELRAVLSDINGGSGVSSEINHQILERFAAAGIVLPGSTRPSRDIYLHGEADMPAPAPKPVPAAAAD
PRKTTPQEAETHDSGEFGPAGMQDDDGGRA
>Mature_829_residues
PRLAALSRLASLILVLFLALNLPALAQQEQEAPDLAAWDKLAEQAEQIVASGSANDVRLQAIREEVVKWRERLKAGQGIN
ATRIATLKDQIAALGTPPAEGQTEPEEIAARRKELNEQLATLQAPGLQAVEAYGRADGIVAQIDQTLRARQTFALIRKTP
TPLNPANWGPAMAEAGHVAGQIYAEARARWDGTGGMSGFTQRLPLIGGFLLVALILLSRGRHWIDSLPSRLSARASERSR
AALIFGVSLGQIAIPLIGVILFAAALASTDLLDDWGLPLLRSVPAAGLSFFGGIWLGRQLFPAADTAVQPPLPMDEERRA
KARFRVTLLASALALHQLFARSILPLSGFNSQNDSGTVPQRLSEASAGVWHFLLILFGAFCLFRLCNLLRGLRPAEAADT
PDYRVRVVSFLAMTGRLAALVAPVLVAVGYVTAGNALLWALVLTLALVGLLVILQDFIADLYALAKGGDQSARDALMPVL
IGFALILLSLPLFALIWGARASDLAEAWTKAQQGFSFGGVRLSPMAMLTFLIVFAAGYFLTNFVQGAFRSSILPKTKLDS
GGQNAVASMIGYVGLALAAVFAVTSAGIDLTSLAFVAGALSVGIGFGMQQVVSNFVSGIILLVERPIAVGDWIEVGGQQG
VVKKMAVRATQIQTFDRTQVIVPNSNLITQPVTNWTRGSLSGRIIVPVTVSHGSDSRKVAELLREIAEDQPTVLINPAPA
VLLRSITADGLNFELRAVLSDINGGSGVSSEINHQILERFAAAGIVLPGSTRPSRDIYLHGEADMPAPAPKPVPAAAADP
RKTTPQEAETHDSGEFGPAGMQDDDGGRA

Specific function: Unknown

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=307, Percent_Identity=29.9674267100977, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI2367355, Length=243, Percent_Identity=31.2757201646091, Blast_Score=133, Evalue=6e-32,
Organism=Escherichia coli, GI1789291, Length=264, Percent_Identity=24.6212121212121, Blast_Score=83, Evalue=8e-17,
Organism=Escherichia coli, GI1787591, Length=132, Percent_Identity=31.8181818181818, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 88113; Mature: 87982

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLAALSRLASLILVLFLALNLPALAQQEQEAPDLAAWDKLAEQAEQIVASGSANDVRL
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
QAIREEVVKWRERLKAGQGINATRIATLKDQIAALGTPPAEGQTEPEEIAARRKELNEQL
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
ATLQAPGLQAVEAYGRADGIVAQIDQTLRARQTFALIRKTPTPLNPANWGPAMAEAGHVA
HHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
GQIYAEARARWDGTGGMSGFTQRLPLIGGFLLVALILLSRGRHWIDSLPSRLSARASERS
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
RAALIFGVSLGQIAIPLIGVILFAAALASTDLLDDWGLPLLRSVPAAGLSFFGGIWLGRQ
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHH
LFPAADTAVQPPLPMDEERRAKARFRVTLLASALALHQLFARSILPLSGFNSQNDSGTVP
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
QRLSEASAGVWHFLLILFGAFCLFRLCNLLRGLRPAEAADTPDYRVRVVSFLAMTGRLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LVAPVLVAVGYVTAGNALLWALVLTLALVGLLVILQDFIADLYALAKGGDQSARDALMPV
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LIGFALILLSLPLFALIWGARASDLAEAWTKAQQGFSFGGVRLSPMAMLTFLIVFAAGYF
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHH
LTNFVQGAFRSSILPKTKLDSGGQNAVASMIGYVGLALAAVFAVTSAGIDLTSLAFVAGA
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LSVGIGFGMQQVVSNFVSGIILLVERPIAVGDWIEVGGQQGVVKKMAVRATQIQTFDRTQ
HHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCE
VIVPNSNLITQPVTNWTRGSLSGRIIVPVTVSHGSDSRKVAELLREIAEDQPTVLINPAP
EEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCH
AVLLRSITADGLNFELRAVLSDINGGSGVSSEINHQILERFAAAGIVLPGSTRPSRDIYL
HHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCEECCCCCCCCCEEEE
HGEADMPAPAPKPVPAAAADPRKTTPQEAETHDSGEFGPAGMQDDDGGRA
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PRLAALSRLASLILVLFLALNLPALAQQEQEAPDLAAWDKLAEQAEQIVASGSANDVRL
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
QAIREEVVKWRERLKAGQGINATRIATLKDQIAALGTPPAEGQTEPEEIAARRKELNEQL
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
ATLQAPGLQAVEAYGRADGIVAQIDQTLRARQTFALIRKTPTPLNPANWGPAMAEAGHVA
HHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
GQIYAEARARWDGTGGMSGFTQRLPLIGGFLLVALILLSRGRHWIDSLPSRLSARASERS
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
RAALIFGVSLGQIAIPLIGVILFAAALASTDLLDDWGLPLLRSVPAAGLSFFGGIWLGRQ
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHH
LFPAADTAVQPPLPMDEERRAKARFRVTLLASALALHQLFARSILPLSGFNSQNDSGTVP
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
QRLSEASAGVWHFLLILFGAFCLFRLCNLLRGLRPAEAADTPDYRVRVVSFLAMTGRLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LVAPVLVAVGYVTAGNALLWALVLTLALVGLLVILQDFIADLYALAKGGDQSARDALMPV
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LIGFALILLSLPLFALIWGARASDLAEAWTKAQQGFSFGGVRLSPMAMLTFLIVFAAGYF
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHH
LTNFVQGAFRSSILPKTKLDSGGQNAVASMIGYVGLALAAVFAVTSAGIDLTSLAFVAGA
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LSVGIGFGMQQVVSNFVSGIILLVERPIAVGDWIEVGGQQGVVKKMAVRATQIQTFDRTQ
HHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCE
VIVPNSNLITQPVTNWTRGSLSGRIIVPVTVSHGSDSRKVAELLREIAEDQPTVLINPAP
EEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCH
AVLLRSITADGLNFELRAVLSDINGGSGVSSEINHQILERFAAAGIVLPGSTRPSRDIYL
HHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCEECCCCCCCCCEEEE
HGEADMPAPAPKPVPAAAADPRKTTPQEAETHDSGEFGPAGMQDDDGGRA
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]