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The map label for this gene is opuAB [H]

Identifier: 119386564

GI number: 119386564

Start: 989964

End: 990845

Strand: Direct

Name: opuAB [H]

Synonym: Pden_3857

Alternate gene names: 119386564

Gene position: 989964-990845 (Clockwise)

Preceding gene: 119386563

Following gene: 119386565

Centisome position: 57.22

GC content: 63.95

Gene sequence:

>882_bases
ATGGACCTGATCCGTTTCCCGGAAATGAGCCGCGGAAACCTGCGCGCGATGCGCCAGACCGTCGACGGCGCCTTCCGCGA
CTTCACCCGCGCCTGGGGGGATACGCTGGAGGCGATCTTTGCGCCGCTGCAAAGCCTGCTGACCTTTATCGAGAACCTGT
TGATCCGATCGCCCTGGATCGCGGTGCTTGCGGTGCTGGCGGCAGGCATCTGGCTGGTCAGCCGAAGCTGGAAGATCGCG
CTGGGATCGGTGCTGGCGCTGCTGGTGGTCGGCTATTTCGGCATGTGGGAGGACACGATGCGCACCATTGCCATGGTTGC
GGTCTGCACCCTGATCGCCGTGGTGCTGGGCATCCCCGTCGGCATCCTCATGGCGCAGTCGAACCGGATGAATGCCATGG
TGACGCCGATCCTGGACATGATGCAGACCATGCCCAGCTTCGTCTACCTGATCCCGGTGGTGATGATCTTTGGCCTGGGC
AAGGTGCCGGGGCTGATCGCGGTGGTGATCTATGCCATCCCGCCGGTGATCCGGCTGACCAACCTGGGCATCCGGCAGGT
GGCGCCCGACGTGCTGGAGGCGGCCGAGGCCTTCGGCTCGTCCAGTTGGCAAAAGCTGCGCGACGTGCAATTGCCGCTGG
CCCTGCCGACGATCATGGCCGGGATCAACCAGACCATCATGATGAGTCTCGCCATGGTCGTCGTCGCCTCGATGATCGGG
GTGCGCGGGTTGGGCCAGCCGGTGCTGCAGGCGATCAACCAGCAATATTTCACCATGGGGGTGATGAACGGCCTGGCCAT
CGTCGCCATCGCCATCATCATGGACCGCACCACGCAGGCCTACGGCAAGCGGCTGCAAAAGCACACGGAGGCCGGCAAAT
GA

Upstream 100 bases:

>100_bases
GACAAGGTCAGGGCCGCCCTTTAATCCGCGCCATCGCGCCTGACGGGCAAGGTCGCGGGTCGCGGCCTTGCCCATTTCCG
TTTCCAAAGGACGAAAAGAC

Downstream 100 bases:

>100_bases
ACAAGGCGGGCACGCATGACATCGAAATCCGCGATCTCTACAAGATCTTCGGCCCCGAGGCCGGGCAGCATGTCGAGGCG
GTCAAGGCCGGGCTGACCAA

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MDLIRFPEMSRGNLRAMRQTVDGAFRDFTRAWGDTLEAIFAPLQSLLTFIENLLIRSPWIAVLAVLAAGIWLVSRSWKIA
LGSVLALLVVGYFGMWEDTMRTIAMVAVCTLIAVVLGIPVGILMAQSNRMNAMVTPILDMMQTMPSFVYLIPVVMIFGLG
KVPGLIAVVIYAIPPVIRLTNLGIRQVAPDVLEAAEAFGSSSWQKLRDVQLPLALPTIMAGINQTIMMSLAMVVVASMIG
VRGLGQPVLQAINQQYFTMGVMNGLAIVAIAIIMDRTTQAYGKRLQKHTEAGK

Sequences:

>Translated_293_residues
MDLIRFPEMSRGNLRAMRQTVDGAFRDFTRAWGDTLEAIFAPLQSLLTFIENLLIRSPWIAVLAVLAAGIWLVSRSWKIA
LGSVLALLVVGYFGMWEDTMRTIAMVAVCTLIAVVLGIPVGILMAQSNRMNAMVTPILDMMQTMPSFVYLIPVVMIFGLG
KVPGLIAVVIYAIPPVIRLTNLGIRQVAPDVLEAAEAFGSSSWQKLRDVQLPLALPTIMAGINQTIMMSLAMVVVASMIG
VRGLGQPVLQAINQQYFTMGVMNGLAIVAIAIIMDRTTQAYGKRLQKHTEAGK
>Mature_293_residues
MDLIRFPEMSRGNLRAMRQTVDGAFRDFTRAWGDTLEAIFAPLQSLLTFIENLLIRSPWIAVLAVLAAGIWLVSRSWKIA
LGSVLALLVVGYFGMWEDTMRTIAMVAVCTLIAVVLGIPVGILMAQSNRMNAMVTPILDMMQTMPSFVYLIPVVMIFGLG
KVPGLIAVVIYAIPPVIRLTNLGIRQVAPDVLEAAEAFGSSSWQKLRDVQLPLALPTIMAGINQTIMMSLAMVVVASMIG
VRGLGQPVLQAINQQYFTMGVMNGLAIVAIAIIMDRTTQAYGKRLQKHTEAGK

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=248, Percent_Identity=44.758064516129, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1788449, Length=192, Percent_Identity=31.25, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788451, Length=179, Percent_Identity=31.8435754189944, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1786564, Length=152, Percent_Identity=29.6052631578947, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31962; Mature: 31962

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
7.2 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
7.2 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLIRFPEMSRGNLRAMRQTVDGAFRDFTRAWGDTLEAIFAPLQSLLTFIENLLIRSPWI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
AVLAVLAAGIWLVSRSWKIALGSVLALLVVGYFGMWEDTMRTIAMVAVCTLIAVVLGIPV
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GILMAQSNRMNAMVTPILDMMQTMPSFVYLIPVVMIFGLGKVPGLIAVVIYAIPPVIRLT
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
NLGIRQVAPDVLEAAEAFGSSSWQKLRDVQLPLALPTIMAGINQTIMMSLAMVVVASMIG
CCCHHHHCHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRGLGQPVLQAINQQYFTMGVMNGLAIVAIAIIMDRTTQAYGKRLQKHTEAGK
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDLIRFPEMSRGNLRAMRQTVDGAFRDFTRAWGDTLEAIFAPLQSLLTFIENLLIRSPWI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
AVLAVLAAGIWLVSRSWKIALGSVLALLVVGYFGMWEDTMRTIAMVAVCTLIAVVLGIPV
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GILMAQSNRMNAMVTPILDMMQTMPSFVYLIPVVMIFGLGKVPGLIAVVIYAIPPVIRLT
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
NLGIRQVAPDVLEAAEAFGSSSWQKLRDVQLPLALPTIMAGINQTIMMSLAMVVVASMIG
CCCHHHHCHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRGLGQPVLQAINQQYFTMGVMNGLAIVAIAIIMDRTTQAYGKRLQKHTEAGK
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]