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The map label for this gene is hphIAM [H]

Identifier: 119386086

GI number: 119386086

Start: 519717

End: 521225

Strand: Direct

Name: hphIAM [H]

Synonym: Pden_3372

Alternate gene names: 119386086

Gene position: 519717-521225 (Clockwise)

Preceding gene: 119386085

Following gene: 119386087

Centisome position: 30.04

GC content: 68.19

Gene sequence:

>1509_bases
ATGACCATGCCGCGTGCGTCTCTTTCCCTGCCGCTGCATGTGCTCGATCTGTTCAGCGGTGCCGCCGGCGGCTGGTCTCT
CGGCCTGCACCGGGCGGGGTTCATGACCATCGCCGCCTGCGAGATCGTGGAATGGCGCCGCATCCTCTATTCGGAGAATT
TTCCGCATGTCAGACTCTACGCCGATATCCGCGACCTCACGGCAACTCGACTTGTTTCCGACCTCGGCTGCCTGCCCGAC
ATCGTCGTCGGCTCGCCGCCCTGCCAGGACATCAGCAGCGCCAACACGAAGGGGAAGGGGATCGAGGGCGAACGCTCGGG
CCTCTACCTCGAGGCCGTGCGCCTGGTCGGAGAGTGCCGCCCTCGCTGGTTCGCTTTTGAGAATAGCTCTAACTTGCGAA
CTCGCGGCGCCGACCGGTTGCTCGATGCGCTGGAAGCGCTCGGCTACGCCTGCTGGCCATGCGTGGTGGGTGCTGCGGAT
ATCGGCGCCAACCATGTCCGCAAGCGGTCCTGGCTCATCGGCTGCGACCCACGGCAGCTTGCCGACACCCGTGTCGCAAT
CCCAGCAGGGCGGCATGCGGCTGGAGGGCGGATCGGGGGCGCGGAAGGCGATGAGGGAGTCGGGGCTTTCCGAGCTGTTC
CGCAATCGCCCGCTGCCGACGCCGATGGCCTCGGACGGGATGAAGGACGGGGCGGGTGGCGGGGCGGGCTCGACATATCC
GCTGCGGATGATCCTGGCGACGCCGCGGAAGACGGACGCGGATCGCGGCGGCCGGGGCGATCTGCTGAGCCAGCTTCAGC
GCCACAACAGCCGCCATGCGGGGATGATGGGCACGCCGACGGCGAATGCGGCGCCACGGGGGAGCGTGCTGCGGAAGCAC
AAGGGCAGGATGACCTCGGATCAGGACCGTTTCGACCGCAGCCCGACGATCGGCGAGGCGCTGCATTTCGACAGACCGCT
GCACCGGGAACCCGAAACCTTACCGACGCCGACCAAGCGCGACAGCCGCATGGACGGCTGGAGTCCGGCCTACGACCGGC
GCAAATCGCCGACCATGGACGCGGTGATGGCCAGAGCGATGGATGGTGCGATGACCGACCGGGCGCCGGACAAATGGGCG
GGCGCGCGGGCTTTGGCGGCGATCTTGCGGAGCCATGGCCTCACTGGAACGGCGGCCTTGCCCATCACATACGGGTGGAT
GATGGGGTTTCCGCCTGGCTGGCTCGCACGCGCATTGCGCTCGGCAATCGCAAGGGGACATCTGCAGCCAGCCTGATCGT
CGAGGCCTTCGGCGATGCGGTGGTGCCGCAGATCCCCGAGGCCATCGGCCGCGCCATCCTGCGCAGCGAGGCCGCGCTGA
ACGCGGTGCTGGGTGGCGCGCCGGCGAGCGATGCTGCCGCCGACGACAGTCCGACCATGAGCACAGTCGCAGGTGATGCC
GCGACCGACGGCGATGCAGTCGGCAACGCGCCCACCGACAATCCTGCGGACCACGGAGGCGCGTCATGA

Upstream 100 bases:

>100_bases
ACTGGCGGCACGTCCACAACCGCATTGCGGCAGGCGAGCCGTTCCGGACCTACGCCAAGGCCCAGCATGCCGCATGGCTG
AAGCGCCGGAGGGTAGGGGC

Downstream 100 bases:

>100_bases
CCCGCAGAAACCGCATCACCTATCTCGCCGCAACCGCTCTCGCGGTTCTGGTGATCGGCGGCATCTCCGTCACCGAGATC
GCGCCCCGGCTGATCTGGAA

Product: C-5 cytosine-specific DNA methylase

Products: NA

Alternate protein names: M.HphIA; Cytosine-specific methyltransferase HphIA; M.Hphi(C) [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MTMPRASLSLPLHVLDLFSGAAGGWSLGLHRAGFMTIAACEIVEWRRILYSENFPHVRLYADIRDLTATRLVSDLGCLPD
IVVGSPPCQDISSANTKGKGIEGERSGLYLEAVRLVGECRPRWFAFENSSNLRTRGADRLLDALEALGYACWPCVVGAAD
IGANHVRKRSWLIGCDPRQLADTRVAIPAGRHAAGGRIGGAEGDEGVGAFRAVPQSPAADADGLGRDEGRGGWRGGLDIS
AADDPGDAAEDGRGSRRPGRSAEPASAPQQPPCGDDGHADGECGATGERAAEAQGQDDLGSGPFRPQPDDRRGAAFRQTA
APGTRNLTDADQARQPHGRLESGLRPAQIADHGRGDGQSDGWCDDRPGAGQMGGRAGFGGDLAEPWPHWNGGLAHHIRVD
DGVSAWLARTRIALGNRKGTSAASLIVEAFGDAVVPQIPEAIGRAILRSEAALNAVLGGAPASDAAADDSPTMSTVAGDA
ATDGDAVGNAPTDNPADHGGAS

Sequences:

>Translated_502_residues
MTMPRASLSLPLHVLDLFSGAAGGWSLGLHRAGFMTIAACEIVEWRRILYSENFPHVRLYADIRDLTATRLVSDLGCLPD
IVVGSPPCQDISSANTKGKGIEGERSGLYLEAVRLVGECRPRWFAFENSSNLRTRGADRLLDALEALGYACWPCVVGAAD
IGANHVRKRSWLIGCDPRQLADTRVAIPAGRHAAGGRIGGAEGDEGVGAFRAVPQSPAADADGLGRDEGRGGWRGGLDIS
AADDPGDAAEDGRGSRRPGRSAEPASAPQQPPCGDDGHADGECGATGERAAEAQGQDDLGSGPFRPQPDDRRGAAFRQTA
APGTRNLTDADQARQPHGRLESGLRPAQIADHGRGDGQSDGWCDDRPGAGQMGGRAGFGGDLAEPWPHWNGGLAHHIRVD
DGVSAWLARTRIALGNRKGTSAASLIVEAFGDAVVPQIPEAIGRAILRSEAALNAVLGGAPASDAAADDSPTMSTVAGDA
ATDGDAVGNAPTDNPADHGGAS
>Mature_501_residues
TMPRASLSLPLHVLDLFSGAAGGWSLGLHRAGFMTIAACEIVEWRRILYSENFPHVRLYADIRDLTATRLVSDLGCLPDI
VVGSPPCQDISSANTKGKGIEGERSGLYLEAVRLVGECRPRWFAFENSSNLRTRGADRLLDALEALGYACWPCVVGAADI
GANHVRKRSWLIGCDPRQLADTRVAIPAGRHAAGGRIGGAEGDEGVGAFRAVPQSPAADADGLGRDEGRGGWRGGLDISA
ADDPGDAAEDGRGSRRPGRSAEPASAPQQPPCGDDGHADGECGATGERAAEAQGQDDLGSGPFRPQPDDRRGAAFRQTAA
PGTRNLTDADQARQPHGRLESGLRPAQIADHGRGDGQSDGWCDDRPGAGQMGGRAGFGGDLAEPWPHWNGGLAHHIRVDD
GVSAWLARTRIALGNRKGTSAASLIVEAFGDAVVPQIPEAIGRAILRSEAALNAVLGGAPASDAAADDSPTMSTVAGDAA
TDGDAVGNAPTDNPADHGGAS

Specific function: This methylase protects the DNA from cleavage by the HphI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 51952; Mature: 51820

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMPRASLSLPLHVLDLFSGAAGGWSLGLHRAGFMTIAACEIVEWRRILYSENFPHVRLY
CCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
ADIRDLTATRLVSDLGCLPDIVVGSPPCQDISSANTKGKGIEGERSGLYLEAVRLVGECR
HHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCC
PRWFAFENSSNLRTRGADRLLDALEALGYACWPCVVGAADIGANHVRKRSWLIGCDPRQL
CCEEEEECCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCEEEECCHHHH
ADTRVAIPAGRHAAGGRIGGAEGDEGVGAFRAVPQSPAADADGLGRDEGRGGWRGGLDIS
CCCEEECCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEC
AADDPGDAAEDGRGSRRPGRSAEPASAPQQPPCGDDGHADGECGATGERAAEAQGQDDLG
CCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCC
SGPFRPQPDDRRGAAFRQTAAPGTRNLTDADQARQPHGRLESGLRPAQIADHGRGDGQSD
CCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHCCCHHHHHHCCCCHHHHHCCCCCCCCC
GWCDDRPGAGQMGGRAGFGGDLAEPWPHWNGGLAHHIRVDDGVSAWLARTRIALGNRKGT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCC
SAASLIVEAFGDAVVPQIPEAIGRAILRSEAALNAVLGGAPASDAAADDSPTMSTVAGDA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCC
ATDGDAVGNAPTDNPADHGGAS
CCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TMPRASLSLPLHVLDLFSGAAGGWSLGLHRAGFMTIAACEIVEWRRILYSENFPHVRLY
CCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
ADIRDLTATRLVSDLGCLPDIVVGSPPCQDISSANTKGKGIEGERSGLYLEAVRLVGECR
HHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCC
PRWFAFENSSNLRTRGADRLLDALEALGYACWPCVVGAADIGANHVRKRSWLIGCDPRQL
CCEEEEECCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCEEEECCHHHH
ADTRVAIPAGRHAAGGRIGGAEGDEGVGAFRAVPQSPAADADGLGRDEGRGGWRGGLDIS
CCCEEECCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEC
AADDPGDAAEDGRGSRRPGRSAEPASAPQQPPCGDDGHADGECGATGERAAEAQGQDDLG
CCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCC
SGPFRPQPDDRRGAAFRQTAAPGTRNLTDADQARQPHGRLESGLRPAQIADHGRGDGQSD
CCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHCCCHHHHHHCCCCHHHHHCCCCCCCCC
GWCDDRPGAGQMGGRAGFGGDLAEPWPHWNGGLAHHIRVDDGVSAWLARTRIALGNRKGT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCC
SAASLIVEAFGDAVVPQIPEAIGRAILRSEAALNAVLGGAPASDAAADDSPTMSTVAGDA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCC
ATDGDAVGNAPTDNPADHGGAS
CCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759008 [H]