Click here to switch to the map view.

The map label for this gene is hyb0 [C]

Identifier: 119385816

GI number: 119385816

Start: 278284

End: 279273

Strand: Direct

Name: hyb0 [C]

Synonym: Pden_3094

Alternate gene names: 119385816

Gene position: 278284-279273 (Clockwise)

Preceding gene: 119385815

Following gene: 119385817

Centisome position: 16.08

GC content: 66.36

Gene sequence:

>990_bases
ATGAACATTCTGTGGCTGCAAAGCGCCGGTTGCGGTGGCTGTACCATGTCGCTGCTATGTGCCGAGGGGCCGAATGTCTT
TGACCTGCTTGCCGGGGCGGGGCTGACCTTCCTGTGGCATCCGGCGCTGAGCCTTGAAAGCGGCACCGAGGTGCGCGGGA
TTCTGGCCGGCCTTGTTTCGGGCGAGGTGCCGCTGGATATTCTCTGCGTCGAGGGCGCGATCGCGCGCGGGCCGCGTGGC
ACCGGACGCTATCAGATCCTGTCGGGCACCGGGCGTCCGTTGCTGGACTGGTTGCGGGATCTGGCGCCGCTGGCGGCCCA
TGTGGTGGCGGTGGGCACCTGTGCGACCTATGGCGGCGTGACCACGGCAGGGGGCAACCCCTCGGATGCGGTGGGGGTGC
AATATGACGGCGCCCATCCGGGCGGCGCCCTGCCGGCGACGTTCCGCGCCCGTGCCGGCCTGCCGGTCATCAATATCGCC
GGCTGCCCGACCCATCCCGATTGGGTGACCGAGACGCTTTTGATGCTGGTGGCGGGCAGGCTGGGGGCCTCGGATCTGGA
TGCGCTTGGCCGGCCCCGGTTCTATGCCGATCATCTGGTCCATCATGCCTGCCCGAAGAACGAGTTCTACGAATACAAGG
CCAGTGCGCGCGCATTGTCGGAAATGGGCTGCATGATGGAGCATCTGGGCTGTATCGGCACACAGGCGGTGGGCGATTGC
AATATCCGGCCCTGGAATGGTCAGGGCAGTTGCACGCGCGGCGGATATCCCTGCATCAACTGCACCGCGCCGGAATTCGA
AGAGCCGCGCCACCTGTTCACCGCCACGCCCAAGATCGCCGGCATTCCGGTTGGCCTGCCGACCGATATGCCCAAGGCCT
GGTTCATGGCGCTGGCCTCGCTGTCCAAGGCCGCCACGCCCGAGCGGATCGCGAAGAATGCCGTGGCCGATCGTATCGTC
ACCCCGCCGACGCTGAGAAAACCCCGATGA

Upstream 100 bases:

>100_bases
TCAAGATCGCCGAGGATCATGGCGGCAGGCTGAGTTACAGCCCCGCCTCCGGTGGTGGCGCGCTGTTTCGGCTGGAACTG
CCGCTGGCAGGAGATGGCGC

Downstream 100 bases:

>100_bases
GCGGGGCAGGGGGCGAGATGGGGCAGGGGCAGTTGATCGTCGGTCCGTTCAACCGGGTCGAGGGCGATCTGGAGATCCGG
CTGGACATCGCAGAGGGGCG

Product: NADH ubiquinone oxidoreductase, 20 kDa subunit

Products: NA

Alternate protein names: NiFeSe hydrogenlyase small chain [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MNILWLQSAGCGGCTMSLLCAEGPNVFDLLAGAGLTFLWHPALSLESGTEVRGILAGLVSGEVPLDILCVEGAIARGPRG
TGRYQILSGTGRPLLDWLRDLAPLAAHVVAVGTCATYGGVTTAGGNPSDAVGVQYDGAHPGGALPATFRARAGLPVINIA
GCPTHPDWVTETLLMLVAGRLGASDLDALGRPRFYADHLVHHACPKNEFYEYKASARALSEMGCMMEHLGCIGTQAVGDC
NIRPWNGQGSCTRGGYPCINCTAPEFEEPRHLFTATPKIAGIPVGLPTDMPKAWFMALASLSKAATPERIAKNAVADRIV
TPPTLRKPR

Sequences:

>Translated_329_residues
MNILWLQSAGCGGCTMSLLCAEGPNVFDLLAGAGLTFLWHPALSLESGTEVRGILAGLVSGEVPLDILCVEGAIARGPRG
TGRYQILSGTGRPLLDWLRDLAPLAAHVVAVGTCATYGGVTTAGGNPSDAVGVQYDGAHPGGALPATFRARAGLPVINIA
GCPTHPDWVTETLLMLVAGRLGASDLDALGRPRFYADHLVHHACPKNEFYEYKASARALSEMGCMMEHLGCIGTQAVGDC
NIRPWNGQGSCTRGGYPCINCTAPEFEEPRHLFTATPKIAGIPVGLPTDMPKAWFMALASLSKAATPERIAKNAVADRIV
TPPTLRKPR
>Mature_329_residues
MNILWLQSAGCGGCTMSLLCAEGPNVFDLLAGAGLTFLWHPALSLESGTEVRGILAGLVSGEVPLDILCVEGAIARGPRG
TGRYQILSGTGRPLLDWLRDLAPLAAHVVAVGTCATYGGVTTAGGNPSDAVGVQYDGAHPGGALPATFRARAGLPVINIA
GCPTHPDWVTETLLMLVAGRLGASDLDALGRPRFYADHLVHHACPKNEFYEYKASARALSEMGCMMEHLGCIGTQAVGDC
NIRPWNGQGSCTRGGYPCINCTAPEFEEPRHLFTATPKIAGIPVGLPTDMPKAWFMALASLSKAATPERIAKNAVADRIV
TPPTLRKPR

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789371, Length=268, Percent_Identity=29.4776119402985, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787206, Length=287, Percent_Identity=22.6480836236934, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 34535; Mature: 34535

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS00277 STAPH_STREP_TOXIN_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILWLQSAGCGGCTMSLLCAEGPNVFDLLAGAGLTFLWHPALSLESGTEVRGILAGLVS
CCEEEEECCCCCCCEEEEEECCCCCHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHC
GEVPLDILCVEGAIARGPRGTGRYQILSGTGRPLLDWLRDLAPLAAHVVAVGTCATYGGV
CCCCEEEEEECCHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCE
TTAGGNPSDAVGVQYDGAHPGGALPATFRARAGLPVINIAGCPTHPDWVTETLLMLVAGR
ECCCCCCCCCEEEEECCCCCCCCCCHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHC
LGASDLDALGRPRFYADHLVHHACPKNEFYEYKASARALSEMGCMMEHLGCIGTQAVGDC
CCCHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
NIRPWNGQGSCTRGGYPCINCTAPEFEEPRHLFTATPKIAGIPVGLPTDMPKAWFMALAS
CCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHH
LSKAATPERIAKNAVADRIVTPPTLRKPR
HHHCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNILWLQSAGCGGCTMSLLCAEGPNVFDLLAGAGLTFLWHPALSLESGTEVRGILAGLVS
CCEEEEECCCCCCCEEEEEECCCCCHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHC
GEVPLDILCVEGAIARGPRGTGRYQILSGTGRPLLDWLRDLAPLAAHVVAVGTCATYGGV
CCCCEEEEEECCHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCE
TTAGGNPSDAVGVQYDGAHPGGALPATFRARAGLPVINIAGCPTHPDWVTETLLMLVAGR
ECCCCCCCCCEEEEECCCCCCCCCCHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHC
LGASDLDALGRPRFYADHLVHHACPKNEFYEYKASARALSEMGCMMEHLGCIGTQAVGDC
CCCHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
NIRPWNGQGSCTRGGYPCINCTAPEFEEPRHLFTATPKIAGIPVGLPTDMPKAWFMALAS
CCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHH
LSKAATPERIAKNAVADRIVTPPTLRKPR
HHHCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3316183; 3322275; 10378275 [H]