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The map label for this gene is mdlY [H]

Identifier: 119385781

GI number: 119385781

Start: 238652

End: 240073

Strand: Direct

Name: mdlY [H]

Synonym: Pden_3059

Alternate gene names: 119385781

Gene position: 238652-240073 (Clockwise)

Preceding gene: 119385780

Following gene: 119385783

Centisome position: 13.79

GC content: 54.78

Gene sequence:

>1422_bases
ATGTCAGCGCTGAAGCCCGTGACCATGACAGAGCAGCTCCGCCAGCTGCGCAGCGGTGAGACGACTGCAGAGGCCATGAT
GACCCGCGCGATCCGGCGGGCCGGAGATTTAGGTCATCTCAATGCCTTTTTGACCGTTGAGCCTGACCTGGCGCTGCAAC
AAGCCCGAGCCGTCGATAGGGCGAGAACAGAGGGGGCGCAGATGCCAATACTGGCGGGACTTCCGATCGGTGTGAAGGAT
AACATCGATGTCGAAGGATGGGTCACTAGCGGTGCCACACCCGCATTGGCCCAGCTGCGTGCAAAGGCGACGGCGCCGGT
TTTGCAGAAGCTGCTTCAGTCTCAGGCAGTTCTGCTAGGTAAAACCAATCTGCACGAACTGGCTTTCGGAGTCACGAATA
CCAACTTTACCCCTATACCGGCGGTCCGAAATCCGCATGATCCGGCCCGGATTAGCGGGGGTTCCTCGGGTGGTACAGCT
GCCGCTATTGCGGCGGGTATTGTCTCTGCCGGGCTTGGAACAGATACCTCTGGTTCTGTTCGCATACCTGCCGCGCTTTG
CGGTATCGCGGGCCTTCGGCCATCGGTCGGCGCGGGAGGCACTGAACGCAGATATTCCTCGCAGGGGGTTCTGCCAATCA
GTCACACCAACGATACGCCCGGCCCGATGGCGGTGTCTGTCGAAGATTTGGCGCTTCTGGACGCTGTAATAACTGGAACT
GATCCGGTCACTGCCAAATCGCTCAAAGGCGTGCGCTTGGGCGTCCCCGATTGTTTCTGGATCGACCTTGATCCTGATGT
TGAACGTGTCTGCCGTGAGCTATGTGGCAAGCTCGCGAAGGCGGGGGCTGAGCTGGTTTCTGTTAGCCTGCCTAGGCTCC
GTGATGTATGGCGGCAGGTATCCGCGCCGGTTGTGCTGCATGAGCCGCGCGAAGATATTCCCGTTTGGCTCGCTGCAAAT
GGCCGATCAGACATCTCTATCATGGATATTTTTCGTCAGATTGTGAACCCTGATGTGCGAGCCGCTTTTGAGGTTGTAAC
CTCGGAAAGCTCTGACAAAAATTATCGTGATGCAATTCAAAATTATCGTCCTGAGCTGTGCCGGATTTATGATGGAGCGT
TTCAAAAAAATGGCCTCGCGGCTTTGATTTTTCCAACGGTTCCAATTCCAGCAACATATATCGACATCGAAAGCGCGTTC
GGAAAAGTAAAGATAAACGGTGAAGATCGGGAGATTTTTGATACGCTTGTTCGAAATACTGATCCGGGTGCTAACGCTGG
ATTGCCGGGATTGAGTATTCCTGTTGGATGGACGCAGAGTGGTTTGCCGGTTGGAATTGAAATTGACGGGCCGGTTGGTT
CGGATCGGGATGTTCTCGGAATTGGAGTTTCAATCGAGCGTGAAATTTCAAATCAAGTGTAA

Upstream 100 bases:

>100_bases
TCGTTGCCTGTGTCGTGTTGATGCCCAGGGGCCTGACCGGCCTGTTCCACGACATCTCGACACGTCTTGCAAAGTCATCT
GGGACCGAAGGAGAGAAATC

Downstream 100 bases:

>100_bases
GTCTGTCGGTTATTTTAATGTGAATTTTTGGTAAAAAACTCAAGGGTGTGGCGCTGAGTGTGCGGCATGACGCATGAATT
GTTTGTCATGAATTTAATTT

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSALKPVTMTEQLRQLRSGETTAEAMMTRAIRRAGDLGHLNAFLTVEPDLALQQARAVDRARTEGAQMPILAGLPIGVKD
NIDVEGWVTSGATPALAQLRAKATAPVLQKLLQSQAVLLGKTNLHELAFGVTNTNFTPIPAVRNPHDPARISGGSSGGTA
AAIAAGIVSAGLGTDTSGSVRIPAALCGIAGLRPSVGAGGTERRYSSQGVLPISHTNDTPGPMAVSVEDLALLDAVITGT
DPVTAKSLKGVRLGVPDCFWIDLDPDVERVCRELCGKLAKAGAELVSVSLPRLRDVWRQVSAPVVLHEPREDIPVWLAAN
GRSDISIMDIFRQIVNPDVRAAFEVVTSESSDKNYRDAIQNYRPELCRIYDGAFQKNGLAALIFPTVPIPATYIDIESAF
GKVKINGEDREIFDTLVRNTDPGANAGLPGLSIPVGWTQSGLPVGIEIDGPVGSDRDVLGIGVSIEREISNQV

Sequences:

>Translated_473_residues
MSALKPVTMTEQLRQLRSGETTAEAMMTRAIRRAGDLGHLNAFLTVEPDLALQQARAVDRARTEGAQMPILAGLPIGVKD
NIDVEGWVTSGATPALAQLRAKATAPVLQKLLQSQAVLLGKTNLHELAFGVTNTNFTPIPAVRNPHDPARISGGSSGGTA
AAIAAGIVSAGLGTDTSGSVRIPAALCGIAGLRPSVGAGGTERRYSSQGVLPISHTNDTPGPMAVSVEDLALLDAVITGT
DPVTAKSLKGVRLGVPDCFWIDLDPDVERVCRELCGKLAKAGAELVSVSLPRLRDVWRQVSAPVVLHEPREDIPVWLAAN
GRSDISIMDIFRQIVNPDVRAAFEVVTSESSDKNYRDAIQNYRPELCRIYDGAFQKNGLAALIFPTVPIPATYIDIESAF
GKVKINGEDREIFDTLVRNTDPGANAGLPGLSIPVGWTQSGLPVGIEIDGPVGSDRDVLGIGVSIEREISNQV
>Mature_472_residues
SALKPVTMTEQLRQLRSGETTAEAMMTRAIRRAGDLGHLNAFLTVEPDLALQQARAVDRARTEGAQMPILAGLPIGVKDN
IDVEGWVTSGATPALAQLRAKATAPVLQKLLQSQAVLLGKTNLHELAFGVTNTNFTPIPAVRNPHDPARISGGSSGGTAA
AIAAGIVSAGLGTDTSGSVRIPAALCGIAGLRPSVGAGGTERRYSSQGVLPISHTNDTPGPMAVSVEDLALLDAVITGTD
PVTAKSLKGVRLGVPDCFWIDLDPDVERVCRELCGKLAKAGAELVSVSLPRLRDVWRQVSAPVVLHEPREDIPVWLAANG
RSDISIMDIFRQIVNPDVRAAFEVVTSESSDKNYRDAIQNYRPELCRIYDGAFQKNGLAALIFPTVPIPATYIDIESAFG
KVKINGEDREIFDTLVRNTDPGANAGLPGLSIPVGWTQSGLPVGIEIDGPVGSDRDVLGIGVSIEREISNQV

Specific function: Hydrolyzes both the R- and the S-enantiomers of mandelamide, and phenylacetamide. Has lower activity on 3- phenylpropionaide and lactamide. Does not hydrolyze benzamide. Hydrolyzes esters and amides with little steric bulk. Preferentially hydrolyzes aroma

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=505, Percent_Identity=27.9207920792079, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI195972892, Length=474, Percent_Identity=24.0506329113924, Blast_Score=87, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17543272, Length=454, Percent_Identity=27.7533039647577, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17556264, Length=186, Percent_Identity=32.7956989247312, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=440, Percent_Identity=27.5, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17537465, Length=505, Percent_Identity=23.1683168316832, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=309, Percent_Identity=27.831715210356, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=368, Percent_Identity=29.0760869565217, Blast_Score=72, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=182, Percent_Identity=34.6153846153846, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=182, Percent_Identity=34.6153846153846, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=441, Percent_Identity=29.0249433106576, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319685, Length=452, Percent_Identity=27.212389380531, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648113, Length=470, Percent_Identity=26.5957446808511, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21356731, Length=457, Percent_Identity=25.382932166302, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24644968, Length=496, Percent_Identity=23.7903225806452, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI161078093, Length=518, Percent_Identity=25.6756756756757, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24652985, Length=508, Percent_Identity=24.4094488188976, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19922090, Length=508, Percent_Identity=24.4094488188976, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24652981, Length=508, Percent_Identity=24.4094488188976, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24652983, Length=508, Percent_Identity=24.4094488188976, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24648435, Length=489, Percent_Identity=25.7668711656442, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648437, Length=489, Percent_Identity=25.7668711656442, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45550774, Length=489, Percent_Identity=25.7668711656442, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648441, Length=439, Percent_Identity=26.1958997722096, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24648439, Length=439, Percent_Identity=26.1958997722096, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.86 [H]

Molecular weight: Translated: 49999; Mature: 49867

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALKPVTMTEQLRQLRSGETTAEAMMTRAIRRAGDLGHLNAFLTVEPDLALQQARAVDR
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHH
ARTEGAQMPILAGLPIGVKDNIDVEGWVTSGATPALAQLRAKATAPVLQKLLQSQAVLLG
HHHCCCCCCEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEC
KTNLHELAFGVTNTNFTPIPAVRNPHDPARISGGSSGGTAAAIAAGIVSAGLGTDTSGSV
CCCHHHHEEEECCCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCE
RIPAALCGIAGLRPSVGAGGTERRYSSQGVLPISHTNDTPGPMAVSVEDLALLDAVITGT
ECHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEEHHHHHHHHHHHCCC
DPVTAKSLKGVRLGVPDCFWIDLDPDVERVCRELCGKLAKAGAELVSVSLPRLRDVWRQV
CCCCHHHCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHH
SAPVVLHEPREDIPVWLAANGRSDISIMDIFRQIVNPDVRAAFEVVTSESSDKNYRDAIQ
CCCEEEECCCCCCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHH
NYRPELCRIYDGAFQKNGLAALIFPTVPIPATYIDIESAFGKVKINGEDREIFDTLVRNT
HCCHHHHHHHCCCCCCCCEEEEEECCCCCCEEEEEEECCCCEEEECCCHHHHHHHHHHCC
DPGANAGLPGLSIPVGWTQSGLPVGIEIDGPVGSDRDVLGIGVSIEREISNQV
CCCCCCCCCCCEECCCCCCCCCEEEEEECCCCCCCCCEEEECCEEEHHHCCCC
>Mature Secondary Structure 
SALKPVTMTEQLRQLRSGETTAEAMMTRAIRRAGDLGHLNAFLTVEPDLALQQARAVDR
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHH
ARTEGAQMPILAGLPIGVKDNIDVEGWVTSGATPALAQLRAKATAPVLQKLLQSQAVLLG
HHHCCCCCCEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEC
KTNLHELAFGVTNTNFTPIPAVRNPHDPARISGGSSGGTAAAIAAGIVSAGLGTDTSGSV
CCCHHHHEEEECCCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCE
RIPAALCGIAGLRPSVGAGGTERRYSSQGVLPISHTNDTPGPMAVSVEDLALLDAVITGT
ECHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEEHHHHHHHHHHHCCC
DPVTAKSLKGVRLGVPDCFWIDLDPDVERVCRELCGKLAKAGAELVSVSLPRLRDVWRQV
CCCCHHHCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHH
SAPVVLHEPREDIPVWLAANGRSDISIMDIFRQIVNPDVRAAFEVVTSESSDKNYRDAIQ
CCCEEEECCCCCCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHH
NYRPELCRIYDGAFQKNGLAALIFPTVPIPATYIDIESAFGKVKINGEDREIFDTLVRNT
HCCHHHHHHHCCCCCCCCEEEEEECCCCCCEEEEEEECCCCEEEECCCHHHHHHHHHHCC
DPGANAGLPGLSIPVGWTQSGLPVGIEIDGPVGSDRDVLGIGVSIEREISNQV
CCCCCCCCCCCEECCCCCCCCCEEEEEECCCCCCCCCEEEECCEEEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12670968 [H]