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The map label for this gene is yhjG [H]

Identifier: 119385770

GI number: 119385770

Start: 225654

End: 227174

Strand: Direct

Name: yhjG [H]

Synonym: Pden_3048

Alternate gene names: 119385770

Gene position: 225654-227174 (Clockwise)

Preceding gene: 119385768

Following gene: 119385771

Centisome position: 13.04

GC content: 67.85

Gene sequence:

>1521_bases
ATGAAAGCGCAAAACCCTGTTGCGGTGCTGATCTGTGGCGCCGGTGCGTCGGGTCTTACCCTGGCGGTCGATCTGGCCCG
TCGTGGCGTGGCCTTCCGCATCATCGAGAAGATGGACCAGCCCTTCGCCGGCTCGCGGGGCAAGGGCATCCAGCCGCGGA
CGCAGGAAGTGTTCGCGGATCTGGGCGTCCTCGACCGTGTGGTGGCGGCAGGCGGTATCTACCCGTCGATGCGGGAATAT
CGCGCGGATGGCACCTGGATCGAGAAAGACATGGCTGAGCGGCATGACCCGACGCCCGCCGAGCCCTATCAGATCGCGCT
GATGGTTCCCCAGTTCCTGACGGAGCGGGTGCTACGCGAGCGTCTGGCCGAGCTTGGCCACCGCGTGGAGTTCGGCACCG
AACTCGTCGGGTTCGAGCAGGACGAGAAGGGCGTCACCGCGCGGCTGGTGGGTCCGGATGGCGAGGAGACCTTAACCGCC
CGCTATCTCGTCGGCGCCGATGGTGGCCGCAGCTTCGTGCGGCGCACGCTTGGGGTGGAGTTCCCCGGCAAGACGCTGGG
CGTGCGCGCCATCGTCGCCGATGTCGCGTTGACCGGCCTTGGACGCGATGCCTGGCATCAGTTCAACGATGGCGACATGC
AGCGGATGGTGGCGATCTGCCCGCTTGCCGGGACCGACCTGTTCCAACTTCAGGCGCCCATCCCGCCGGAGGGCGAAGCC
GATCTCTCTGCCAAGGCGCTGGAGGGAATGATACTGGATCGGACAGGCCGCAGCGATATCCGGGTGCATTCCGTGGCCTG
GGCTTCCGCCTATGCCATGAACGCGCGGCTCGCCGAATGCTACCGGGTCGGCCGGGCGTTCCTGGTGGGGGACGCCGCCC
ATATCCATCCGCCGACCGGCGGTCAGGGCCTGAACACCAGCGTGCAGGATGCCTGGAACCTCGGGTGGAAGCTGGCAGCC
GTGCTGCATGGCGCGCCGGATGGGCTGCTCGACACCTACGAGGCGGAGCGGCGCCCGGTGGCCGAAGCGATGCTGGGGCT
TGCGACCGGCCTTCTTGACGCGATGAAACGCGGAATGACGCGGCGCGGCCGCGAGACCCGCCAGCTCGACATCGGATACC
CCGAGTCGCCGCTGGCCCTCGATCCCTCCGGACGGCCCGGTATCCTGCTCGCCGGGGATCGCGCGCCCGACGCTCCGCTA
CGCGGCGCTGCTGGACTGTCCCGCCGCCTGTTCGACCTGTTCAGCGGCCCTCACTGGACGCTGATCGGCTTCGAGGCTGA
GCGCGACATCATTGCGCCGAGAGGCGGGCTGCACATCCACCGGGTCGGCCCCGGCCAGGAGCTTGAGGACGAGGACGGCC
ACCTGCACGCCGCCTATGGCCTGGCACCCGGCGAATGGGTGCTGGTGCGTCCCGACGGCTATGTCGCCGCGATCGTGTCC
ATGCAAGATGTGGCTCTTTTGGAGAGTTTCCTTGCCGAGGTAGGAGTGGCGGGAGCACGCTCTGGAGACCTGAAATCGTA
G

Upstream 100 bases:

>100_bases
CAGCTCGGGCAACATCTTCCTGATATGTGTATGATGCACTTTTATCGTTGACACGCGCAGACCCTCCATAATATGTGCAT
CATACACATTGGAGAGAACG

Downstream 100 bases:

>100_bases
CCCCGTGATCTGTTGACATTGATCGCTTGAAATTCAGTTCATGCTGGTGGTTTCGAGCGCAAATATGGGCGTTTGCCTAC
GCGGCTGTTCGAAATATCTC

Product: hypothetical protein

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MKAQNPVAVLICGAGASGLTLAVDLARRGVAFRIIEKMDQPFAGSRGKGIQPRTQEVFADLGVLDRVVAAGGIYPSMREY
RADGTWIEKDMAERHDPTPAEPYQIALMVPQFLTERVLRERLAELGHRVEFGTELVGFEQDEKGVTARLVGPDGEETLTA
RYLVGADGGRSFVRRTLGVEFPGKTLGVRAIVADVALTGLGRDAWHQFNDGDMQRMVAICPLAGTDLFQLQAPIPPEGEA
DLSAKALEGMILDRTGRSDIRVHSVAWASAYAMNARLAECYRVGRAFLVGDAAHIHPPTGGQGLNTSVQDAWNLGWKLAA
VLHGAPDGLLDTYEAERRPVAEAMLGLATGLLDAMKRGMTRRGRETRQLDIGYPESPLALDPSGRPGILLAGDRAPDAPL
RGAAGLSRRLFDLFSGPHWTLIGFEAERDIIAPRGGLHIHRVGPGQELEDEDGHLHAAYGLAPGEWVLVRPDGYVAAIVS
MQDVALLESFLAEVGVAGARSGDLKS

Sequences:

>Translated_506_residues
MKAQNPVAVLICGAGASGLTLAVDLARRGVAFRIIEKMDQPFAGSRGKGIQPRTQEVFADLGVLDRVVAAGGIYPSMREY
RADGTWIEKDMAERHDPTPAEPYQIALMVPQFLTERVLRERLAELGHRVEFGTELVGFEQDEKGVTARLVGPDGEETLTA
RYLVGADGGRSFVRRTLGVEFPGKTLGVRAIVADVALTGLGRDAWHQFNDGDMQRMVAICPLAGTDLFQLQAPIPPEGEA
DLSAKALEGMILDRTGRSDIRVHSVAWASAYAMNARLAECYRVGRAFLVGDAAHIHPPTGGQGLNTSVQDAWNLGWKLAA
VLHGAPDGLLDTYEAERRPVAEAMLGLATGLLDAMKRGMTRRGRETRQLDIGYPESPLALDPSGRPGILLAGDRAPDAPL
RGAAGLSRRLFDLFSGPHWTLIGFEAERDIIAPRGGLHIHRVGPGQELEDEDGHLHAAYGLAPGEWVLVRPDGYVAAIVS
MQDVALLESFLAEVGVAGARSGDLKS
>Mature_506_residues
MKAQNPVAVLICGAGASGLTLAVDLARRGVAFRIIEKMDQPFAGSRGKGIQPRTQEVFADLGVLDRVVAAGGIYPSMREY
RADGTWIEKDMAERHDPTPAEPYQIALMVPQFLTERVLRERLAELGHRVEFGTELVGFEQDEKGVTARLVGPDGEETLTA
RYLVGADGGRSFVRRTLGVEFPGKTLGVRAIVADVALTGLGRDAWHQFNDGDMQRMVAICPLAGTDLFQLQAPIPPEGEA
DLSAKALEGMILDRTGRSDIRVHSVAWASAYAMNARLAECYRVGRAFLVGDAAHIHPPTGGQGLNTSVQDAWNLGWKLAA
VLHGAPDGLLDTYEAERRPVAEAMLGLATGLLDAMKRGMTRRGRETRQLDIGYPESPLALDPSGRPGILLAGDRAPDAPL
RGAAGLSRRLFDLFSGPHWTLIGFEAERDIIAPRGGLHIHRVGPGQELEDEDGHLHAAYGLAPGEWVLVRPDGYVAAIVS
MQDVALLESFLAEVGVAGARSGDLKS

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=346, Percent_Identity=34.6820809248555, Blast_Score=149, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 54389; Mature: 54389

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAQNPVAVLICGAGASGLTLAVDLARRGVAFRIIEKMDQPFAGSRGKGIQPRTQEVFAD
CCCCCCEEEEEECCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
LGVLDRVVAAGGIYPSMREYRADGTWIEKDMAERHDPTPAEPYQIALMVPQFLTERVLRE
HHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHH
RLAELGHRVEFGTELVGFEQDEKGVTARLVGPDGEETLTARYLVGADGGRSFVRRTLGVE
HHHHHCCCCCCCCHHCCCCCCCCCCEEEEECCCCCCEEEEEEEEECCCCHHHHHHHHCCC
FPGKTLGVRAIVADVALTGLGRDAWHQFNDGDMQRMVAICPLAGTDLFQLQAPIPPEGEA
CCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCC
DLSAKALEGMILDRTGRSDIRVHSVAWASAYAMNARLAECYRVGRAFLVGDAAHIHPPTG
CCHHHHHCCEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCEEEEECCCEECCCCC
GQGLNTSVQDAWNLGWKLAAVLHGAPDGLLDTYEAERRPVAEAMLGLATGLLDAMKRGMT
CCCCCCCHHHHHCCCCEEEHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
RRGRETRQLDIGYPESPLALDPSGRPGILLAGDRAPDAPLRGAAGLSRRLFDLFSGPHWT
HCCCCCCEEECCCCCCCEEECCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEE
LIGFEAERDIIAPRGGLHIHRVGPGQELEDEDGHLHAAYGLAPGEWVLVRPDGYVAAIVS
EEEEECCCCEECCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEECCCCEEEEEEH
MQDVALLESFLAEVGVAGARSGDLKS
HHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKAQNPVAVLICGAGASGLTLAVDLARRGVAFRIIEKMDQPFAGSRGKGIQPRTQEVFAD
CCCCCCEEEEEECCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
LGVLDRVVAAGGIYPSMREYRADGTWIEKDMAERHDPTPAEPYQIALMVPQFLTERVLRE
HHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHH
RLAELGHRVEFGTELVGFEQDEKGVTARLVGPDGEETLTARYLVGADGGRSFVRRTLGVE
HHHHHCCCCCCCCHHCCCCCCCCCCEEEEECCCCCCEEEEEEEEECCCCHHHHHHHHCCC
FPGKTLGVRAIVADVALTGLGRDAWHQFNDGDMQRMVAICPLAGTDLFQLQAPIPPEGEA
CCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCC
DLSAKALEGMILDRTGRSDIRVHSVAWASAYAMNARLAECYRVGRAFLVGDAAHIHPPTG
CCHHHHHCCEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCEEEEECCCEECCCCC
GQGLNTSVQDAWNLGWKLAAVLHGAPDGLLDTYEAERRPVAEAMLGLATGLLDAMKRGMT
CCCCCCCHHHHHCCCCEEEHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
RRGRETRQLDIGYPESPLALDPSGRPGILLAGDRAPDAPLRGAAGLSRRLFDLFSGPHWT
HCCCCCCEEECCCCCCCEEECCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEE
LIGFEAERDIIAPRGGLHIHRVGPGQELEDEDGHLHAAYGLAPGEWVLVRPDGYVAAIVS
EEEEECCCCEECCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEECCCCEEEEEEH
MQDVALLESFLAEVGVAGARSGDLKS
HHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]