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The map label for this gene is bioB2

Identifier: 119385641

GI number: 119385641

Start: 79269

End: 80231

Strand: Direct

Name: bioB2

Synonym: Pden_2916

Alternate gene names: 119385641

Gene position: 79269-80231 (Clockwise)

Preceding gene: 119385640

Following gene: 119385642

Centisome position: 4.58

GC content: 66.77

Gene sequence:

>963_bases
ATGCCCCAGCAATCGCGCAGCGCCGCCGAGATCTATCACCAGCCGCTGATGGACCTGCTGTTTCAGGCCCAGACCGTGCA
TCGGGCGCATTTCGACCCGAATGTCGTGCAATGTTCGAAGCTCTTGTCGATCAAGACCGGCGGCTGCCCCGAGGATTGCG
CCTATTGCTCGCAATCGGCGCGCAACGGCTCGGAACTGTCGGCCTCGAAACTGATGGAGGTGCAGCGCGTGCTGGCCGAG
GCCAGGCGCGCCAAGGAGGCGGGGGCGACGCGCTATTGCATGGGCGCCGCCTGGCGCTCGCCCAAGGAGCGCGACATGCC
GGCGGTGCTGGCGATGATCCGCGGCGTCAAGGCGATGGGGATGGAAACCTGCATGACGCTGGGCATGCTGGACGCGGACC
AGGCGCTGCGGCTGAAGGACGCGGGGCTGGATTACTACAACCACAATATCGACACCTCGGAGCGCTATTATTCCGAGATC
ATCACCACCCGGACCTTCCAGGACCGGATCGAGACGCTGGAGCGGGTGCAGGCGGCGGGCATCAATGTCTGCGCCGGCGG
CATCGTCGGCATGGGCGAGACGGCCGAGGACCGCATCTCGATGCTGGAGACGCTGGCCGGGCTGGAGGTGCCGCCGCAAT
CGGTGCCGATCAACATGCTGATGCCGATGGCGGGCACGCCGCTGGCCGATGTGCCCAGGCTGGACGCGATCGAGATGGTG
CGCACCATCGCCACGGCGCGCATCCTGATGCCGGCATCCTATGTGCGGCTGTCGGCCGGGCGGTCCGAGATGAGCGACGA
GATGCAGGCCATGTGCTTTTTTGCCGGGGCCAATTCGATCTTTGTCGGCGACACGCTGCTGACCGCCGGGAACCCGGACG
AGGACAAGGACGCGCTGCTGTTCGCCAAGCTGGGGCTGCGGGCCGAGGTGCCGGAGGCGTCGCAGGAGGGCTGCGCGGCA
TGA

Upstream 100 bases:

>100_bases
GTCCGTGATCGTTTCTGAAATAATAGATAATATATACGGATTAACAAAGCGGCGCTGACCGTTTTATAGATACTTCCACA
TGAGGCTCAGGGAGGTATCC

Downstream 100 bases:

>100_bases
GCTTTTCGCGGCACGGGGCGGCGCTGGAGGCGATGGCGCGGCGCGGACGGCTGCGCCGGGTGGCGCCGGCGGCGGGTGCC
GATTTCGCCTCGAACGACTA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MPQQSRSAAEIYHQPLMDLLFQAQTVHRAHFDPNVVQCSKLLSIKTGGCPEDCAYCSQSARNGSELSASKLMEVQRVLAE
ARRAKEAGATRYCMGAAWRSPKERDMPAVLAMIRGVKAMGMETCMTLGMLDADQALRLKDAGLDYYNHNIDTSERYYSEI
ITTRTFQDRIETLERVQAAGINVCAGGIVGMGETAEDRISMLETLAGLEVPPQSVPINMLMPMAGTPLADVPRLDAIEMV
RTIATARILMPASYVRLSAGRSEMSDEMQAMCFFAGANSIFVGDTLLTAGNPDEDKDALLFAKLGLRAEVPEASQEGCAA

Sequences:

>Translated_320_residues
MPQQSRSAAEIYHQPLMDLLFQAQTVHRAHFDPNVVQCSKLLSIKTGGCPEDCAYCSQSARNGSELSASKLMEVQRVLAE
ARRAKEAGATRYCMGAAWRSPKERDMPAVLAMIRGVKAMGMETCMTLGMLDADQALRLKDAGLDYYNHNIDTSERYYSEI
ITTRTFQDRIETLERVQAAGINVCAGGIVGMGETAEDRISMLETLAGLEVPPQSVPINMLMPMAGTPLADVPRLDAIEMV
RTIATARILMPASYVRLSAGRSEMSDEMQAMCFFAGANSIFVGDTLLTAGNPDEDKDALLFAKLGLRAEVPEASQEGCAA
>Mature_319_residues
PQQSRSAAEIYHQPLMDLLFQAQTVHRAHFDPNVVQCSKLLSIKTGGCPEDCAYCSQSARNGSELSASKLMEVQRVLAEA
RRAKEAGATRYCMGAAWRSPKERDMPAVLAMIRGVKAMGMETCMTLGMLDADQALRLKDAGLDYYNHNIDTSERYYSEII
TTRTFQDRIETLERVQAAGINVCAGGIVGMGETAEDRISMLETLAGLEVPPQSVPINMLMPMAGTPLADVPRLDAIEMVR
TIATARILMPASYVRLSAGRSEMSDEMQAMCFFAGANSIFVGDTLLTAGNPDEDKDALLFAKLGLRAEVPEASQEGCAA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=300, Percent_Identity=63.3333333333333, Blast_Score=412, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6321725, Length=306, Percent_Identity=49.3464052287582, Blast_Score=317, Evalue=1e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB2_PARDP (A1B656)

Other databases:

- EMBL:   CP000490
- RefSeq:   YP_916696.1
- ProteinModelPortal:   A1B656
- SMR:   A1B656
- STRING:   A1B656
- GeneID:   4581483
- GenomeReviews:   CP000490_GR
- KEGG:   pde:Pden_2916
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   A1B656
- ProtClustDB:   CLSK2783200
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 34800; Mature: 34669

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
9.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
8.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQQSRSAAEIYHQPLMDLLFQAQTVHRAHFDPNVVQCSKLLSIKTGGCPEDCAYCSQSA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHC
RNGSELSASKLMEVQRVLAEARRAKEAGATRYCMGAAWRSPKERDMPAVLAMIRGVKAMG
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
METCMTLGMLDADQALRLKDAGLDYYNHNIDTSERYYSEIITTRTFQDRIETLERVQAAG
HHHHHHHCCCCCHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
INVCAGGIVGMGETAEDRISMLETLAGLEVPPQSVPINMLMPMAGTPLADVPRLDAIEMV
CCEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHEECCCCCCCCCCCCCCHHHHHH
RTIATARILMPASYVRLSAGRSEMSDEMQAMCFFAGANSIFVGDTLLTAGNPDEDKDALL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCEEEECCCCCCCHHHHH
FAKLGLRAEVPEASQEGCAA
HHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PQQSRSAAEIYHQPLMDLLFQAQTVHRAHFDPNVVQCSKLLSIKTGGCPEDCAYCSQSA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHC
RNGSELSASKLMEVQRVLAEARRAKEAGATRYCMGAAWRSPKERDMPAVLAMIRGVKAMG
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
METCMTLGMLDADQALRLKDAGLDYYNHNIDTSERYYSEIITTRTFQDRIETLERVQAAG
HHHHHHHCCCCCHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
INVCAGGIVGMGETAEDRISMLETLAGLEVPPQSVPINMLMPMAGTPLADVPRLDAIEMV
CCEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHEECCCCCCCCCCCCCCHHHHHH
RTIATARILMPASYVRLSAGRSEMSDEMQAMCFFAGANSIFVGDTLLTAGNPDEDKDALL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCEEEECCCCCCCHHHHH
FAKLGLRAEVPEASQEGCAA
HHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA