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The map label for this gene is exoA [H]

Identifier: 119358281

GI number: 119358281

Start: 2877660

End: 2878430

Strand: Reverse

Name: exoA [H]

Synonym: Cpha266_2513

Alternate gene names: 119358281

Gene position: 2878430-2877660 (Counterclockwise)

Preceding gene: 119358282

Following gene: 119358280

Centisome position: 91.85

GC content: 51.75

Gene sequence:

>771_bases
ATGAAAATAGCCTCGTGGAATGTCAATGGTATCCGGGCCCGTCAGGAGGCTCTTGTTTCGTGGATTGGACGCAATTCGCC
GGACGTTCTCGTTCTGCAGGAGGTTAAGGCTGATCTTGCCGATATTCCCGCCTCCATCAGCGAACTCGAGGGCTATGACA
GTTTCTGGAACGGATCTTCGTTTCGTAAGGGATACAGCGGTACCGGTATGCTTGTCCGGAAGGGCGCGTTTTCCGGCACG
GTTGAATGGGAGATACCGGCATTCGATTTTGAAAACAGAACCTGCGTTCTTCACACCGGGCAGTTTACCCTTACAGGATG
CTATGTTCCTCGGGGGGAGGGGGATGAACATTACCGTCTGAAGCTTCGCTACTTTGAGCAGACAAGACTTTTTATTGAAA
ACCTTCTGCTTGAGGGGCGTCAGATTGTTTTTACCGGTGATATGAATGTGGCTCATCGGGATATCGACGTTCATCGCTCC
CAGAACAAGCCTGGCGCCGTTGGTTTGAGAGCTGAAGAGCGGTCTTCGATCGATGCCTGTCTTGCTCTTGGGCTTCGGGA
CGTCATGAGAGAGAGAGCCCCGGAGCGTAACGATCTGTTTACCTGGTGGCCATACTGGAAAAATGCCCGTGAGAGAAATC
TCGGGTGGCGTATTGACTGTTTTTTTCTTTCTGCCGCAATCGCCGAAAGGGTAACGATGGTCGCTGTTGATCGCGATGAA
AGAAGTTCGGATCATGCGCCGATTATTCTTGAGCTTTCCGACCTCTCCTGA

Upstream 100 bases:

>100_bases
GTCGCGATCGATGCCCGAAAGGCCATTTTCGTTGTATGAATGAGTTGTCTGAATCGCTGATTGCCAAAAAGATTGTCGAC
TATTTTAACACTGAACGGTC

Downstream 100 bases:

>100_bases
TCGCTCGGGATTCTCTCTGCCATCTCATCGAGCCGATCACAAAAATGAATAAGAGAACAGGCAATCATAGCATGTTCTTT
GCTGCTCGGGGTATAGGGCA

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MKIASWNVNGIRARQEALVSWIGRNSPDVLVLQEVKADLADIPASISELEGYDSFWNGSSFRKGYSGTGMLVRKGAFSGT
VEWEIPAFDFENRTCVLHTGQFTLTGCYVPRGEGDEHYRLKLRYFEQTRLFIENLLLEGRQIVFTGDMNVAHRDIDVHRS
QNKPGAVGLRAEERSSIDACLALGLRDVMRERAPERNDLFTWWPYWKNARERNLGWRIDCFFLSAAIAERVTMVAVDRDE
RSSDHAPIILELSDLS

Sequences:

>Translated_256_residues
MKIASWNVNGIRARQEALVSWIGRNSPDVLVLQEVKADLADIPASISELEGYDSFWNGSSFRKGYSGTGMLVRKGAFSGT
VEWEIPAFDFENRTCVLHTGQFTLTGCYVPRGEGDEHYRLKLRYFEQTRLFIENLLLEGRQIVFTGDMNVAHRDIDVHRS
QNKPGAVGLRAEERSSIDACLALGLRDVMRERAPERNDLFTWWPYWKNARERNLGWRIDCFFLSAAIAERVTMVAVDRDE
RSSDHAPIILELSDLS
>Mature_256_residues
MKIASWNVNGIRARQEALVSWIGRNSPDVLVLQEVKADLADIPASISELEGYDSFWNGSSFRKGYSGTGMLVRKGAFSGT
VEWEIPAFDFENRTCVLHTGQFTLTGCYVPRGEGDEHYRLKLRYFEQTRLFIENLLLEGRQIVFTGDMNVAHRDIDVHRS
QNKPGAVGLRAEERSSIDACLALGLRDVMRERAPERNDLFTWWPYWKNARERNLGWRIDCFFLSAAIAERVTMVAVDRDE
RSSDHAPIILELSDLS

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=257, Percent_Identity=33.4630350194552, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI18375503, Length=257, Percent_Identity=33.4630350194552, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI18375501, Length=257, Percent_Identity=33.4630350194552, Blast_Score=142, Evalue=3e-34,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=28.0898876404494, Blast_Score=116, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71989536, Length=257, Percent_Identity=27.2373540856031, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI221330655, Length=259, Percent_Identity=31.6602316602317, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17136678, Length=259, Percent_Identity=31.6602316602317, Blast_Score=115, Evalue=4e-26,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29218; Mature: 29218

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIASWNVNGIRARQEALVSWIGRNSPDVLVLQEVKADLADIPASISELEGYDSFWNGSS
CEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCHHHHCCCCCCCCCCC
FRKGYSGTGMLVRKGAFSGTVEWEIPAFDFENRTCVLHTGQFTLTGCYVPRGEGDEHYRL
CCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCEEEEECCCEEEEEEEEECCCCCCEEEE
KLRYFEQTRLFIENLLLEGRQIVFTGDMNVAHRDIDVHRSQNKPGAVGLRAEERSSIDAC
EEEEHHHHHHHHHHHHHCCCEEEEECCCCEEECCEEEECCCCCCCEEEECCHHHHHHHHH
LALGLRDVMRERAPERNDLFTWWPYWKNARERNLGWRIDCFFLSAAIAERVTMVAVDRDE
HHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHCEEEEEEECCC
RSSDHAPIILELSDLS
CCCCCCCEEEEECCCC
>Mature Secondary Structure
MKIASWNVNGIRARQEALVSWIGRNSPDVLVLQEVKADLADIPASISELEGYDSFWNGSS
CEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCHHHHCCCCCCCCCCC
FRKGYSGTGMLVRKGAFSGTVEWEIPAFDFENRTCVLHTGQFTLTGCYVPRGEGDEHYRL
CCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCEEEEECCCEEEEEEEEECCCCCCEEEE
KLRYFEQTRLFIENLLLEGRQIVFTGDMNVAHRDIDVHRSQNKPGAVGLRAEERSSIDAC
EEEEHHHHHHHHHHHHHCCCEEEEECCCCEEECCEEEECCCCCCCEEEECCHHHHHHHHH
LALGLRDVMRERAPERNDLFTWWPYWKNARERNLGWRIDCFFLSAAIAERVTMVAVDRDE
HHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHCEEEEEEECCC
RSSDHAPIILELSDLS
CCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]