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The map label for this gene is miaB [H]

Identifier: 119358129

GI number: 119358129

Start: 2746059

End: 2747387

Strand: Reverse

Name: miaB [H]

Synonym: Cpha266_2361

Alternate gene names: 119358129

Gene position: 2747387-2746059 (Counterclockwise)

Preceding gene: 119358130

Following gene: 119358128

Centisome position: 87.67

GC content: 51.77

Gene sequence:

>1329_bases
ATGCCCAGAGCTCGCAGAAAATTCTATATCCATACTTTTGGATGCCAGATGAATCAGGCCGACAGCGGTATAATTACGGC
ACTCCTGGAAAAAGAGGGATATCAGCAGGCCGCTTCGGAAGAGGAGGCAGGCATCATTATGCTCAATACCTGTGCGGTCA
GGGAGAACGCCGTTGAACGCATTAGCCACTACCTTCAGCATGTGAAGGGGTTCAAGAGAAAGTGCCCCGAACTGATTGTC
GGTCTCACCGGCTGTATTCCCCAGTATCGCAGGGAAGAACTGTTTACCGTTTTTCCCGTGATCGATTTTCTTGCGGGCCC
CGACACCTATCGAGTTCTTCCCGCACTGATTGCCGAGGCAGGAGATAGCCGGTCGGCACGGCTTGAATTCAATGCTTTCG
AGACTTATGATGGCGTCAGACAGGCAAGAACGCAATCACTCAACGCTTTTGTGCCGATTATTCGGGGCTGCAACAACATG
TGTGCTTTCTGCGTCGTTCCATTCACCAGAGGACGTGAACGCAGTCACCCGTTCGAATCGGTTCTCGACGAGGTTCGTGC
GCTTGCCGACGACGGCTGCAGAGAGATAACGCTGCTTGGTCAGAACGTCAATTCATACCATGATCCTGCATCAGGTGCAG
ATTTTTCCCGGTTGCTCGATGCGGTCAGTCGGGAAGCTCCCGAAACGCGAATACGGTTTACTACCTCCCATCCGAAGGAT
ATGTCGCACTCGCTTGTGGAAACGATGGCATTACGGCCGAATATCTGTAATCATCTTCATCTGCCGGTGCAGTCCGGTTC
GACGCGTATGCTTGCTCGAATGAACAGAGGACACGACATCGAGGAGTACCGGAATAAAATAGAGCTTCTTCGCAAATGGA
TTCCTGGTATATCGCTTTCTACCGATCTTATCGCGGGCTTTTGCGGCGAAAGTGAAGCTGATCATGACCAAACGCTCGAA
CTGATGCGCGAAGTCCGTTTCGATTCGGTCTTCATGTTTTACTATTCCGTCAGACAGGGAACACTTGCAGCCCGAACCAT
GCCCGATGATGTGCCTGAAGAGGTTAAAAAGCAGCGGCTGCAGGAGATTATCGATCTGCAGAATGGTATTTCTGCGGAGC
TGCTTGGCCTGGCGCCTGGTTCTGTCGTTGAAGTTCTTGTCGAATCGGAAAGTCGTCGTTCTTCCGATCAGCTTATGGGA
AGAACTGACGGCAACAGGGTTGTGGTATTCGACCGGGGCATCCATCAACCCGGAGATTTGGTCAGGGTTATGATTACCGG
GTCGACTTCGGCAACTCTTTTCGGTCAGTCGGCAGAGAATCTGCAATGA

Upstream 100 bases:

>100_bases
TGGGATAACGGATCCATAACCGGGCGCAGAATGTGTGCCTGGTTTTTTAAAGCCATGCCTGTCGCACATTCCGCTTCAGA
GCAGAAGATAACCAGTAACG

Downstream 100 bases:

>100_bases
AATCGTAACCCATGAATAGTATCAGCAGATAAAAACCTTATCTGCATTCAGGAGTTTTTTTATCTTTACATCGATTTGTA
AATTTAAGTTTGAGTGAACG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MPRARRKFYIHTFGCQMNQADSGIITALLEKEGYQQAASEEEAGIIMLNTCAVRENAVERISHYLQHVKGFKRKCPELIV
GLTGCIPQYRREELFTVFPVIDFLAGPDTYRVLPALIAEAGDSRSARLEFNAFETYDGVRQARTQSLNAFVPIIRGCNNM
CAFCVVPFTRGRERSHPFESVLDEVRALADDGCREITLLGQNVNSYHDPASGADFSRLLDAVSREAPETRIRFTTSHPKD
MSHSLVETMALRPNICNHLHLPVQSGSTRMLARMNRGHDIEEYRNKIELLRKWIPGISLSTDLIAGFCGESEADHDQTLE
LMREVRFDSVFMFYYSVRQGTLAARTMPDDVPEEVKKQRLQEIIDLQNGISAELLGLAPGSVVEVLVESESRRSSDQLMG
RTDGNRVVVFDRGIHQPGDLVRVMITGSTSATLFGQSAENLQ

Sequences:

>Translated_442_residues
MPRARRKFYIHTFGCQMNQADSGIITALLEKEGYQQAASEEEAGIIMLNTCAVRENAVERISHYLQHVKGFKRKCPELIV
GLTGCIPQYRREELFTVFPVIDFLAGPDTYRVLPALIAEAGDSRSARLEFNAFETYDGVRQARTQSLNAFVPIIRGCNNM
CAFCVVPFTRGRERSHPFESVLDEVRALADDGCREITLLGQNVNSYHDPASGADFSRLLDAVSREAPETRIRFTTSHPKD
MSHSLVETMALRPNICNHLHLPVQSGSTRMLARMNRGHDIEEYRNKIELLRKWIPGISLSTDLIAGFCGESEADHDQTLE
LMREVRFDSVFMFYYSVRQGTLAARTMPDDVPEEVKKQRLQEIIDLQNGISAELLGLAPGSVVEVLVESESRRSSDQLMG
RTDGNRVVVFDRGIHQPGDLVRVMITGSTSATLFGQSAENLQ
>Mature_441_residues
PRARRKFYIHTFGCQMNQADSGIITALLEKEGYQQAASEEEAGIIMLNTCAVRENAVERISHYLQHVKGFKRKCPELIVG
LTGCIPQYRREELFTVFPVIDFLAGPDTYRVLPALIAEAGDSRSARLEFNAFETYDGVRQARTQSLNAFVPIIRGCNNMC
AFCVVPFTRGRERSHPFESVLDEVRALADDGCREITLLGQNVNSYHDPASGADFSRLLDAVSREAPETRIRFTTSHPKDM
SHSLVETMALRPNICNHLHLPVQSGSTRMLARMNRGHDIEEYRNKIELLRKWIPGISLSTDLIAGFCGESEADHDQTLEL
MREVRFDSVFMFYYSVRQGTLAARTMPDDVPEEVKKQRLQEIIDLQNGISAELLGLAPGSVVEVLVESESRRSSDQLMGR
TDGNRVVVFDRGIHQPGDLVRVMITGSTSATLFGQSAENLQ

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=476, Percent_Identity=42.8571428571429, Blast_Score=374, Evalue=1e-103,
Organism=Homo sapiens, GI28872782, Length=476, Percent_Identity=42.8571428571429, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI93277076, Length=373, Percent_Identity=27.3458445040214, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1786882, Length=442, Percent_Identity=41.1764705882353, Blast_Score=304, Evalue=7e-84,
Organism=Escherichia coli, GI1787057, Length=446, Percent_Identity=28.0269058295964, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17553146, Length=460, Percent_Identity=40, Blast_Score=330, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI71996771, Length=225, Percent_Identity=28, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356207, Length=481, Percent_Identity=38.2536382536383, Blast_Score=332, Evalue=3e-91,
Organism=Drosophila melanogaster, GI19922432, Length=447, Percent_Identity=26.3982102908277, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49589; Mature: 49458

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRARRKFYIHTFGCQMNQADSGIITALLEKEGYQQAASEEEAGIIMLNTCAVRENAVER
CCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHH
ISHYLQHVKGFKRKCPELIVGLTGCIPQYRREELFTVFPVIDFLAGPDTYRVLPALIAEA
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
GDSRSARLEFNAFETYDGVRQARTQSLNAFVPIIRGCNNMCAFCVVPFTRGRERSHPFES
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHH
VLDEVRALADDGCREITLLGQNVNSYHDPASGADFSRLLDAVSREAPETRIRFTTSHPKD
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCH
MSHSLVETMALRPNICNHLHLPVQSGSTRMLARMNRGHDIEEYRNKIELLRKWIPGISLS
HHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCC
TDLIAGFCGESEADHDQTLELMREVRFDSVFMFYYSVRQGTLAARTMPDDVPEEVKKQRL
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHH
QEIIDLQNGISAELLGLAPGSVVEVLVESESRRSSDQLMGRTDGNRVVVFDRGIHQPGDL
HHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEEECCCCCCCCE
VRVMITGSTSATLFGQSAENLQ
EEEEEECCCCEEEECCCCCCCC
>Mature Secondary Structure 
PRARRKFYIHTFGCQMNQADSGIITALLEKEGYQQAASEEEAGIIMLNTCAVRENAVER
CCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHH
ISHYLQHVKGFKRKCPELIVGLTGCIPQYRREELFTVFPVIDFLAGPDTYRVLPALIAEA
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
GDSRSARLEFNAFETYDGVRQARTQSLNAFVPIIRGCNNMCAFCVVPFTRGRERSHPFES
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHH
VLDEVRALADDGCREITLLGQNVNSYHDPASGADFSRLLDAVSREAPETRIRFTTSHPKD
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCH
MSHSLVETMALRPNICNHLHLPVQSGSTRMLARMNRGHDIEEYRNKIELLRKWIPGISLS
HHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCC
TDLIAGFCGESEADHDQTLELMREVRFDSVFMFYYSVRQGTLAARTMPDDVPEEVKKQRL
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHH
QEIIDLQNGISAELLGLAPGSVVEVLVESESRRSSDQLMGRTDGNRVVVFDRGIHQPGDL
HHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEEECCCCCCCCE
VRVMITGSTSATLFGQSAENLQ
EEEEEECCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA