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The map label for this gene is luxO [H]

Identifier: 119357682

GI number: 119357682

Start: 2182683

End: 2183822

Strand: Direct

Name: luxO [H]

Synonym: Cpha266_1886

Alternate gene names: 119357682

Gene position: 2182683-2183822 (Clockwise)

Preceding gene: 119357681

Following gene: 119357684

Centisome position: 69.65

GC content: 47.89

Gene sequence:

>1140_bases
ATGAAACGCAATAGCTCGTTTATTGGAAAATCACAACTGGTCACCCAGCTCAGGGAACTCGCACTGCAGGTTGCCGATAC
GGAAATCACTGTCCTTATCATCGGAGAGACCGGGGCAGGAAAAGAGGTACTTGCCCGGTTCATCCATGCCAACAGCCGAC
GTGCTGATAAAAGTTTTATTCCCGTCAACTGCGGAGCCATCCCGTCGGGAATTCTTGAATCAGAGCTTTTCGGACATGAA
AAAGGGGCTTTTACCGGAGCTTTTCAAAGCAGAAAAGGGTATTTCGAAAGTGCCGACAGGGGAACAATTTTTCTTGATGA
AATCGGTGAAATGCCGCTTGAAACACAGGTCAAGTTTCTTCGGGTTCTTGAAACAGGAGAGTTTCAGCGGGTAGGATCTT
CGGATTCCGTTTTTGCCGATACACGAATCATTGCGGCAACGAACAAAAACCTCTATCAGGCTGTCGCTGACGGCAACTTC
CGCGAAGATCTCTACTACCGCCTGCGCAGTGTGGAACTCCAGATCCCACCGCTCAGAGAACGAGGCCCCGATATTTTGCT
CCTCGCAGAACATTTCATTCACGAGTTCGAACGAAAACACCGGGTAATATTTGAAGGATTCACCCCTGAATCTGCAGAAC
TCATGCTTCGCTATCCATGGCCCGGAAATATCAGAGAGCTGAGAAATCTTGTCGAATCCCTGCTCATTCTTGAAAAAGGA
AAAAGAATAACAACGGAAATTCTTGAAAAACATCTCATCCAGCGCAACCGCTACAAAAGTCTCGTGCACGAACCGACAAA
ACAGGAAAACCAGGAACTCAATATCATCTACCGAAGCCTGATCCAGCTCCGTCAGGACATTAACGACATCAGGCAGCTTC
TTGAACGTCTTATTGAAGATCGTCAGGAGAGACCGCTGCTGCTTCCCGACACCACTGCGCACGTAGCGCCAGCCGGAAAT
CAAATCGTTGAAGAGAGAGCCTCGGAAACATCATCAACCGTGAGTTCTCTGGATGAACTGGAAAAAAAAACCATTGCCGA
AACGCTTGCCGCCTGCGAAGGAAACAAACGAAAAACAGCAGGAATCCTTGGCATAACTGAACGAACGCTCTATCGAAAAA
TCAAAACCTACGACCTTTAG

Upstream 100 bases:

>100_bases
AAAAGAGGCCATTAGCGCCGCCACGACTATTCCATTCACGAGCATCGTTGTTCCTGTGAACTGATACATCTATTTCCGAA
CCCATGCAGCAGCAAACAAC

Downstream 100 bases:

>100_bases
ACCGAAGTTCCGGATTCAGGCGAAGCCAGAGAAAACCAACTGCCAATTCTCTTTTTCGTGTAAATTCGTGTCCATTCGCG
GGCAAACTCTTCCTCTTCAT

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MKRNSSFIGKSQLVTQLRELALQVADTEITVLIIGETGAGKEVLARFIHANSRRADKSFIPVNCGAIPSGILESELFGHE
KGAFTGAFQSRKGYFESADRGTIFLDEIGEMPLETQVKFLRVLETGEFQRVGSSDSVFADTRIIAATNKNLYQAVADGNF
REDLYYRLRSVELQIPPLRERGPDILLLAEHFIHEFERKHRVIFEGFTPESAELMLRYPWPGNIRELRNLVESLLILEKG
KRITTEILEKHLIQRNRYKSLVHEPTKQENQELNIIYRSLIQLRQDINDIRQLLERLIEDRQERPLLLPDTTAHVAPAGN
QIVEERASETSSTVSSLDELEKKTIAETLAACEGNKRKTAGILGITERTLYRKIKTYDL

Sequences:

>Translated_379_residues
MKRNSSFIGKSQLVTQLRELALQVADTEITVLIIGETGAGKEVLARFIHANSRRADKSFIPVNCGAIPSGILESELFGHE
KGAFTGAFQSRKGYFESADRGTIFLDEIGEMPLETQVKFLRVLETGEFQRVGSSDSVFADTRIIAATNKNLYQAVADGNF
REDLYYRLRSVELQIPPLRERGPDILLLAEHFIHEFERKHRVIFEGFTPESAELMLRYPWPGNIRELRNLVESLLILEKG
KRITTEILEKHLIQRNRYKSLVHEPTKQENQELNIIYRSLIQLRQDINDIRQLLERLIEDRQERPLLLPDTTAHVAPAGN
QIVEERASETSSTVSSLDELEKKTIAETLAACEGNKRKTAGILGITERTLYRKIKTYDL
>Mature_379_residues
MKRNSSFIGKSQLVTQLRELALQVADTEITVLIIGETGAGKEVLARFIHANSRRADKSFIPVNCGAIPSGILESELFGHE
KGAFTGAFQSRKGYFESADRGTIFLDEIGEMPLETQVKFLRVLETGEFQRVGSSDSVFADTRIIAATNKNLYQAVADGNF
REDLYYRLRSVELQIPPLRERGPDILLLAEHFIHEFERKHRVIFEGFTPESAELMLRYPWPGNIRELRNLVESLLILEKG
KRITTEILEKHLIQRNRYKSLVHEPTKQENQELNIIYRSLIQLRQDINDIRQLLERLIEDRQERPLLLPDTTAHVAPAGN
QIVEERASETSSTVSSLDELEKKTIAETLAACEGNKRKTAGILGITERTLYRKIKTYDL

Specific function: Acts negatively to control the expression of luminescence. At low cell density, luxO is phosphorylated, and together with sigma-54, causes repression of the luxCDABEGH operon. This repression could be indirect, luxO could activate a negative regulator of

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=357, Percent_Identity=39.2156862745098, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI87082117, Length=276, Percent_Identity=44.9275362318841, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI1789233, Length=375, Percent_Identity=37.8666666666667, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1790437, Length=239, Percent_Identity=50.2092050209205, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1788905, Length=247, Percent_Identity=44.5344129554656, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI1789087, Length=235, Percent_Identity=47.2340425531915, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1790299, Length=373, Percent_Identity=36.7292225201072, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI87082152, Length=246, Percent_Identity=43.9024390243902, Blast_Score=214, Evalue=6e-57,
Organism=Escherichia coli, GI87081872, Length=229, Percent_Identity=45.8515283842795, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1786524, Length=244, Percent_Identity=47.1311475409836, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1787583, Length=227, Percent_Identity=41.4096916299559, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1789828, Length=275, Percent_Identity=34.9090909090909, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87081858, Length=230, Percent_Identity=30.8695652173913, Blast_Score=109, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 43203; Mature: 43203

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRNSSFIGKSQLVTQLRELALQVADTEITVLIIGETGAGKEVLARFIHANSRRADKSFI
CCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCC
PVNCGAIPSGILESELFGHEKGAFTGAFQSRKGYFESADRGTIFLDEIGEMPLETQVKFL
EEECCCCCCHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCEEEHHHHCCCCHHHHHHHH
RVLETGEFQRVGSSDSVFADTRIIAATNKNLYQAVADGNFREDLYYRLRSVELQIPPLRE
HHHHCCCHHCCCCCCCEEECCEEEEECCCHHHHHHHCCCHHHHHHHHHHHCEEECCCHHH
RGPDILLLAEHFIHEFERKHRVIFEGFTPESAELMLRYPWPGNIRELRNLVESLLILEKG
CCCCEEEHHHHHHHHHHHHCEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCC
KRITTEILEKHLIQRNRYKSLVHEPTKQENQELNIIYRSLIQLRQDINDIRQLLERLIED
CHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQERPLLLPDTTAHVAPAGNQIVEERASETSSTVSSLDELEKKTIAETLAACEGNKRKTA
HHCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GILGITERTLYRKIKTYDL
CEEEHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRNSSFIGKSQLVTQLRELALQVADTEITVLIIGETGAGKEVLARFIHANSRRADKSFI
CCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCC
PVNCGAIPSGILESELFGHEKGAFTGAFQSRKGYFESADRGTIFLDEIGEMPLETQVKFL
EEECCCCCCHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCEEEHHHHCCCCHHHHHHHH
RVLETGEFQRVGSSDSVFADTRIIAATNKNLYQAVADGNFREDLYYRLRSVELQIPPLRE
HHHHCCCHHCCCCCCCEEECCEEEEECCCHHHHHHHCCCHHHHHHHHHHHCEEECCCHHH
RGPDILLLAEHFIHEFERKHRVIFEGFTPESAELMLRYPWPGNIRELRNLVESLLILEKG
CCCCEEEHHHHHHHHHHHHCEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCC
KRITTEILEKHLIQRNRYKSLVHEPTKQENQELNIIYRSLIQLRQDINDIRQLLERLIED
CHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQERPLLLPDTTAHVAPAGNQIVEERASETSSTVSSLDELEKKTIAETLAACEGNKRKTA
HHCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GILGITERTLYRKIKTYDL
CEEEHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8065259 [H]