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Identifier: 119357657

GI number: 119357657

Start: 2156396

End: 2156962

Strand: Reverse

Name: def

Synonym: Cpha266_1861

Alternate gene names: 119357657

Gene position: 2156962-2156396 (Counterclockwise)

Preceding gene: 119357658

Following gene: 119357656

Centisome position: 68.83

GC content: 51.32

Gene sequence:

>567_bases
ATGATATTGCCGATCAACATCTATAACGATGAGGTACTGAGGGCAACCGCAAAACCCTTGAAGGGCATTGATCGCAACAT
CCGGGATCTTGTTGCGAGCATGCTTGAGTCCATGCGCAACGCATCGGGAATAGGACTTGCCGCACCGCAGGTCGGTTGCT
CGATCCGGTTGCTGGTGATTGATCTGTCCTGTATGGAGAAATATGCAGATGAAAAGCCGGTGGTTGTGATCAATCCGCAC
CTGCTTGCCGTCAAGGGTTACAATGCGATGGAAGAGGGGTGTCTCAGTCTGCCTGGCATTCTTGGCGACGTTGTTCGTCC
ATCCTCGATCACCCTGAAATACCGCGATGAACATTTCGAGGAACGAACCGGTGAGTTTTCGGGAATGATGGCGCGGGTAC
TCCAGCACGAGATAGATCATCTCGATGGCAAGCTGTTTATTGACAGGATGCAGAAACGTGACCGGAGAAAAATAGACAAA
GAGCTTCACTCCCTTGCAATCGGCAATGTTGAGGCGGATTATCCTCTGGCACTGGCAGGTATTGCTCAAAAGGCCTGTGA
GGCTTGA

Upstream 100 bases:

>100_bases
AGAGATTCTTTTCGCGCTTGCTGGCATTGATTAAAAGGATTTCTCCGGAGAGCCGAGCCGGTGTTGCCGGTAACAACAAC
AATGATTAGTTTCCAAAACA

Downstream 100 bases:

>100_bases
AGTCTTTTCTGCCGCTTGATCAAGCGGCATTCAACCCTGTTTCAACATTCTCAATCTCATGCGTATCGTCTTTATGGGAA
CTCCGGAATTTGCGGTTCCC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MILPINIYNDEVLRATAKPLKGIDRNIRDLVASMLESMRNASGIGLAAPQVGCSIRLLVIDLSCMEKYADEKPVVVINPH
LLAVKGYNAMEEGCLSLPGILGDVVRPSSITLKYRDEHFEERTGEFSGMMARVLQHEIDHLDGKLFIDRMQKRDRRKIDK
ELHSLAIGNVEADYPLALAGIAQKACEA

Sequences:

>Translated_188_residues
MILPINIYNDEVLRATAKPLKGIDRNIRDLVASMLESMRNASGIGLAAPQVGCSIRLLVIDLSCMEKYADEKPVVVINPH
LLAVKGYNAMEEGCLSLPGILGDVVRPSSITLKYRDEHFEERTGEFSGMMARVLQHEIDHLDGKLFIDRMQKRDRRKIDK
ELHSLAIGNVEADYPLALAGIAQKACEA
>Mature_188_residues
MILPINIYNDEVLRATAKPLKGIDRNIRDLVASMLESMRNASGIGLAAPQVGCSIRLLVIDLSCMEKYADEKPVVVINPH
LLAVKGYNAMEEGCLSLPGILGDVVRPSSITLKYRDEHFEERTGEFSGMMARVLQHEIDHLDGKLFIDRMQKRDRRKIDK
ELHSLAIGNVEADYPLALAGIAQKACEA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=161, Percent_Identity=31.055900621118, Blast_Score=75, Evalue=3e-14,
Organism=Escherichia coli, GI1789682, Length=155, Percent_Identity=40.6451612903226, Blast_Score=102, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24645728, Length=157, Percent_Identity=33.1210191082803, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24645726, Length=140, Percent_Identity=31.4285714285714, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_CHLPD (A1BHK0)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_912301.1
- ProteinModelPortal:   A1BHK0
- SMR:   A1BHK0
- STRING:   A1BHK0
- GeneID:   4571203
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_1861
- NMPDR:   fig|290317.7.peg.1958
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   MILPINI
- ProtClustDB:   PRK14598
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 20870; Mature: 20870

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: ACT_SITE 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILPINIYNDEVLRATAKPLKGIDRNIRDLVASMLESMRNASGIGLAAPQVGCSIRLLVI
CEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEE
DLSCMEKYADEKPVVVINPHLLAVKGYNAMEEGCLSLPGILGDVVRPSSITLKYRDEHFE
EHHHHHHHCCCCCEEEECCCEEEEECCCHHHHHHHHCCHHHHHHCCCCCEEEEECHHHHH
ERTGEFSGMMARVLQHEIDHLDGKLFIDRMQKRDRRKIDKELHSLAIGNVEADYPLALAG
HHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
IAQKACEA
HHHHHHCC
>Mature Secondary Structure
MILPINIYNDEVLRATAKPLKGIDRNIRDLVASMLESMRNASGIGLAAPQVGCSIRLLVI
CEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEE
DLSCMEKYADEKPVVVINPHLLAVKGYNAMEEGCLSLPGILGDVVRPSSITLKYRDEHFE
EHHHHHHHCCCCCEEEECCCEEEEECCCHHHHHHHHCCHHHHHHCCCCCEEEEECHHHHH
ERTGEFSGMMARVLQHEIDHLDGKLFIDRMQKRDRRKIDKELHSLAIGNVEADYPLALAG
HHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
IAQKACEA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA