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The map label for this gene is appA [H]

Identifier: 119357586

GI number: 119357586

Start: 2040468

End: 2042186

Strand: Direct

Name: appA [H]

Synonym: Cpha266_1789

Alternate gene names: 119357586

Gene position: 2040468-2042186 (Clockwise)

Preceding gene: 119357585

Following gene: 119357587

Centisome position: 65.11

GC content: 48.34

Gene sequence:

>1719_bases
ATGCTCCAACCTGATTTCCGTACAACACTGCGAGTCGCTACAACGTTGTTGTACTCATCGCTCATTGTGTTCATGCTCGC
CTCTCTTTCTGCCTGTCGCCAAAACAGTGAAAACTCCCGCAAAGATCATATCGTGGCCGGAGTATCGGCCGACTTCGATT
ACCTTAACCCCCTGCTCATCCAGCTCTCCCTCTCAAGGGAGGTGTGTTCGCTGATCTACCCCGCACTGGTCAAACCTGAA
TACAATGAAAAAAAAGGAACCATTGACTATCAGCCATCTGCGGCTGAACGATGGGAATTCTCTTCCGACGGTAAAACCGT
GATCTTTCACCTCCGCCCTGATGCCATTTGGGAAGACGGAAAAAAAGTAACATCACGCGATTTCAAGTTTTCTTATTCAC
TGTATAAAAACCCTGCAATTGCCAGCTCCCGCCAGCACTATCTTGATGATCTGCTTTTACTACCCGACGGATCTCCTGAT
ATCGACAAAAGCGTAGAAACGCCTGATGAGACAACTCTGATTCTTCATTTCAGAAAACCGATGGATCCCGACATCGTGCT
TGACCACTTCAACGATCTCATGCCCGTCGCCGAACATCTTTTCAGAACAGTTCCTCCTGGTGAAATCCGAAGCAGAGCTG
CAGAACTTCCGATAACAGGAGCCGGACCGTTCAAGGTTAAAGAGTGGTCTCGTCAGCAGAAACTCGTACTGATTTCCAGT
CCCACTGCTGTTCTTCCCCATCCTGCAGTATCCAAAACCATGACATTCCTTATTGTTCCTGAATATACCACCCGCCTGGC
CATGCTCAAATCAGGACAGATAGACGCCATGATCTCTTCCGGCGGCATCAATCCGAGGGATGTCCCTGAACTTCTGAAAA
CAAACCCTGATATCACCATCAAACCACTGAAAAACCGCTATTTCGACAGTGTAGTCTGGCTTGCTATCGATGGCGAGACA
TACAGAAATACAGGAAGCATTGTGCCGAACCGGTTTTTCGGAAACAGGAAAGTTCGCCAGGCAATGACCTTTGCCATTGA
CCGCCAGGCCATTATTGATGGATTCATGGGACCTGAACATGCCGCTATTGTCAACACATCACTATCACCGGCATACACAA
CAATCAGGGATACCACAAGCGAATCGTATGCCTTCAATCCCGAAAAAGCAAAATCGCTGCTGCGCGAGGAGGGGTGGATC
CAGGGACCTGACGGTATTCTGCAAAAAAACGGGGTACGATTCTCTTTTGAACTTGCCGCACAGGTCGGCAATCCACGTCG
AAACTATGCGGCAACCATCATCCAGCAGAACTTGCGGGATGTCGGTATCGACTGCCGTCTGAAGTTTGATGAAAGTCTTG
TTTTTCTGAAAAGCCAGAATGAATTTCGCTATGATGCCGCACTTTCAGGACTTGCGGCTGAAACTCTGCCGTTCCAGCTT
GTCATCTGGGGTTCGGATTTCTCCTCAAGACCTTTCAACTCTTCGGCTTTTCAGAACAGGGAGCTTGATGCCGTTATTGC
CGAACTGAGCCAACCACTGGATCTTCAGAAGAAACAACGGCTATGGATAACCTATCAGCAAATTCTGACCGAAGAGCAAC
CCCGATCATTTCTCTACTATTACGACGAGCTTGAAGGCTTCAGTAAACGCCTTGAAAACGTCAAGGTGAACCTGATCGCA
ACGCTCTACAACGCTTACGAATGGAGTCTGAAAAACTGA

Upstream 100 bases:

>100_bases
AAGAGAAAAAGGCATAATCCTTTTTTCCGGATTCAAAAAAATACGACTCGCCCGTTAACCGGGCAGCAAGATAACGTCAC
AACTTCTCAATCAATACGTT

Downstream 100 bases:

>100_bases
GTATAACATGGAGACGGTTGCCTGTTTGCCGCTCTCTCCAATTTTACTATATTCAACTACTTTTTTCAAAAGCAGTTATG
GCGTCAGTAACCATCTCATG

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MLQPDFRTTLRVATTLLYSSLIVFMLASLSACRQNSENSRKDHIVAGVSADFDYLNPLLIQLSLSREVCSLIYPALVKPE
YNEKKGTIDYQPSAAERWEFSSDGKTVIFHLRPDAIWEDGKKVTSRDFKFSYSLYKNPAIASSRQHYLDDLLLLPDGSPD
IDKSVETPDETTLILHFRKPMDPDIVLDHFNDLMPVAEHLFRTVPPGEIRSRAAELPITGAGPFKVKEWSRQQKLVLISS
PTAVLPHPAVSKTMTFLIVPEYTTRLAMLKSGQIDAMISSGGINPRDVPELLKTNPDITIKPLKNRYFDSVVWLAIDGET
YRNTGSIVPNRFFGNRKVRQAMTFAIDRQAIIDGFMGPEHAAIVNTSLSPAYTTIRDTTSESYAFNPEKAKSLLREEGWI
QGPDGILQKNGVRFSFELAAQVGNPRRNYAATIIQQNLRDVGIDCRLKFDESLVFLKSQNEFRYDAALSGLAAETLPFQL
VIWGSDFSSRPFNSSAFQNRELDAVIAELSQPLDLQKKQRLWITYQQILTEEQPRSFLYYYDELEGFSKRLENVKVNLIA
TLYNAYEWSLKN

Sequences:

>Translated_572_residues
MLQPDFRTTLRVATTLLYSSLIVFMLASLSACRQNSENSRKDHIVAGVSADFDYLNPLLIQLSLSREVCSLIYPALVKPE
YNEKKGTIDYQPSAAERWEFSSDGKTVIFHLRPDAIWEDGKKVTSRDFKFSYSLYKNPAIASSRQHYLDDLLLLPDGSPD
IDKSVETPDETTLILHFRKPMDPDIVLDHFNDLMPVAEHLFRTVPPGEIRSRAAELPITGAGPFKVKEWSRQQKLVLISS
PTAVLPHPAVSKTMTFLIVPEYTTRLAMLKSGQIDAMISSGGINPRDVPELLKTNPDITIKPLKNRYFDSVVWLAIDGET
YRNTGSIVPNRFFGNRKVRQAMTFAIDRQAIIDGFMGPEHAAIVNTSLSPAYTTIRDTTSESYAFNPEKAKSLLREEGWI
QGPDGILQKNGVRFSFELAAQVGNPRRNYAATIIQQNLRDVGIDCRLKFDESLVFLKSQNEFRYDAALSGLAAETLPFQL
VIWGSDFSSRPFNSSAFQNRELDAVIAELSQPLDLQKKQRLWITYQQILTEEQPRSFLYYYDELEGFSKRLENVKVNLIA
TLYNAYEWSLKN
>Mature_572_residues
MLQPDFRTTLRVATTLLYSSLIVFMLASLSACRQNSENSRKDHIVAGVSADFDYLNPLLIQLSLSREVCSLIYPALVKPE
YNEKKGTIDYQPSAAERWEFSSDGKTVIFHLRPDAIWEDGKKVTSRDFKFSYSLYKNPAIASSRQHYLDDLLLLPDGSPD
IDKSVETPDETTLILHFRKPMDPDIVLDHFNDLMPVAEHLFRTVPPGEIRSRAAELPITGAGPFKVKEWSRQQKLVLISS
PTAVLPHPAVSKTMTFLIVPEYTTRLAMLKSGQIDAMISSGGINPRDVPELLKTNPDITIKPLKNRYFDSVVWLAIDGET
YRNTGSIVPNRFFGNRKVRQAMTFAIDRQAIIDGFMGPEHAAIVNTSLSPAYTTIRDTTSESYAFNPEKAKSLLREEGWI
QGPDGILQKNGVRFSFELAAQVGNPRRNYAATIIQQNLRDVGIDCRLKFDESLVFLKSQNEFRYDAALSGLAAETLPFQL
VIWGSDFSSRPFNSSAFQNRELDAVIAELSQPLDLQKKQRLWITYQQILTEEQPRSFLYYYDELEGFSKRLENVKVNLIA
TLYNAYEWSLKN

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=511, Percent_Identity=22.8962818003914, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787495, Length=465, Percent_Identity=24.5161290322581, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1789887, Length=542, Percent_Identity=24.3542435424354, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1789397, Length=514, Percent_Identity=21.5953307392996, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787762, Length=531, Percent_Identity=22.2222222222222, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787052, Length=531, Percent_Identity=22.9755178907721, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 64865; Mature: 64865

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQPDFRTTLRVATTLLYSSLIVFMLASLSACRQNSENSRKDHIVAGVSADFDYLNPLLI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHCCEEE
QLSLSREVCSLIYPALVKPEYNEKKGTIDYQPSAAERWEFSSDGKTVIFHLRPDAIWEDG
EEECCHHHHHHHHHHHCCCCCCCCCCCEECCCCCHHCCEECCCCCEEEEEECCCHHHCCC
KKVTSRDFKFSYSLYKNPAIASSRQHYLDDLLLLPDGSPDIDKSVETPDETTLILHFRKP
CCCCCCCEEEEEEECCCCCCCCCHHHHHHCEEECCCCCCCCCCCCCCCCCEEEEEEECCC
MDPDIVLDHFNDLMPVAEHLFRTVPPGEIRSRAAELPITGAGPFKVKEWSRQQKLVLISS
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEC
PTAVLPHPAVSKTMTFLIVPEYTTRLAMLKSGQIDAMISSGGINPRDVPELLKTNPDITI
CCCCCCCCCHHHCEEEEEECCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCEEE
KPLKNRYFDSVVWLAIDGETYRNTGSIVPNRFFGNRKVRQAMTFAIDRQAIIDGFMGPEH
EECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
AAIVNTSLSPAYTTIRDTTSESYAFNPEKAKSLLREEGWIQGPDGILQKNGVRFSFELAA
EEEEECCCCCCEEEEECCCCCCCEECHHHHHHHHHHCCCCCCCCCHHHCCCCEEEEEEEE
QVGNPRRNYAATIIQQNLRDVGIDCRLKFDESLVFLKSQNEFRYDAALSGLAAETLPFQL
CCCCCCHHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCCCEEHHHHHHCCHHCCCCEEE
VIWGSDFSSRPFNSSAFQNRELDAVIAELSQPLDLQKKQRLWITYQQILTEEQPRSFLYY
EEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHEEHHHHHCCCCCCCEEEE
YDELEGFSKRLENVKVNLIATLYNAYEWSLKN
HHHHHHHHHHHHCCEEEEEEEEHHHHEECCCC
>Mature Secondary Structure
MLQPDFRTTLRVATTLLYSSLIVFMLASLSACRQNSENSRKDHIVAGVSADFDYLNPLLI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHCCEEE
QLSLSREVCSLIYPALVKPEYNEKKGTIDYQPSAAERWEFSSDGKTVIFHLRPDAIWEDG
EEECCHHHHHHHHHHHCCCCCCCCCCCEECCCCCHHCCEECCCCCEEEEEECCCHHHCCC
KKVTSRDFKFSYSLYKNPAIASSRQHYLDDLLLLPDGSPDIDKSVETPDETTLILHFRKP
CCCCCCCEEEEEEECCCCCCCCCHHHHHHCEEECCCCCCCCCCCCCCCCCEEEEEEECCC
MDPDIVLDHFNDLMPVAEHLFRTVPPGEIRSRAAELPITGAGPFKVKEWSRQQKLVLISS
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEC
PTAVLPHPAVSKTMTFLIVPEYTTRLAMLKSGQIDAMISSGGINPRDVPELLKTNPDITI
CCCCCCCCCHHHCEEEEEECCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCEEE
KPLKNRYFDSVVWLAIDGETYRNTGSIVPNRFFGNRKVRQAMTFAIDRQAIIDGFMGPEH
EECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
AAIVNTSLSPAYTTIRDTTSESYAFNPEKAKSLLREEGWIQGPDGILQKNGVRFSFELAA
EEEEECCCCCCEEEEECCCCCCCEECHHHHHHHHHHCCCCCCCCCHHHCCCCEEEEEEEE
QVGNPRRNYAATIIQQNLRDVGIDCRLKFDESLVFLKSQNEFRYDAALSGLAAETLPFQL
CCCCCCHHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCCCEEHHHHHHCCHHCCCCEEE
VIWGSDFSSRPFNSSAFQNRELDAVIAELSQPLDLQKKQRLWITYQQILTEEQPRSFLYY
EEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHEEHHHHHCCCCCCCEEEE
YDELEGFSKRLENVKVNLIATLYNAYEWSLKN
HHHHHHHHHHHHCCEEEEEEEEHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]