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The map label for this gene is galE [C]

Identifier: 119357538

GI number: 119357538

Start: 1966817

End: 1967845

Strand: Reverse

Name: galE [C]

Synonym: Cpha266_1741

Alternate gene names: 119357538

Gene position: 1967845-1966817 (Counterclockwise)

Preceding gene: 119357542

Following gene: 119357534

Centisome position: 62.79

GC content: 48.88

Gene sequence:

>1029_bases
ATGAATGTACTGGTTACCGGTGCGGCAGGATTTATCGGTTTTCATGTTTGCCGGAGGCTGCTTGACAGAGGCGACAGCGT
TACTGGCCTTGATAATATGAATGATTATTATGATGTTCATCTTAAAGAGTCCCGTCTTGCGCAGCTTGAGCCGGAAGAGG
CATTTTCTTTTGTGAAAATGGATCTTGCCGACAGGGCAGGAATGGAAGAGCTTTTTGATGCCAGCAGGTTCGACAGGGTG
ATCAATCTTGCTGCTCAGGCCGGGGTGCGCTACTCACTCATCAATCCATACTCCTATATTGACAGCAATATCCAGGGTTT
TTTGAATATTCTCGAAGGTTGTCGCCATAATGGCATTGAGCATCTGGTTTATGCCTCTTCGAGTTCAGTTTATGGCGCAA
ACGAATCCATGCCGTTTTCAGTACATGATAATGTGGATCATCCTTTGTCGCTGTATGCTGCAAGCAAAAAGGCAAATGAG
CTGATGGCTCATACCTATAGTCATCTGTACGGTATTCCATCAACCGGGTTGCGTTTTTTTACAGTGTATGGTCCATGGGG
ACGACCCGATATGGCGCTTTTTCTGTTTACCGAAGCCATTCTTGCCGGCAGACCGATAGAGGTGTTCAACTTCGGAAACC
ATCGAAGGGATTTTACCTACATTGATGATATTGTCGAGGGAGTGCTCAGGACGCTTGATCATCCAGCCGAACCCAATCCG
GACTGGACTGGTCTCAAGCCCGATCCCGGAACGAGCAGAGCTCCCTGGAGGGTCTACAATATCGGTAACAGCCGCCCGGT
GAACCTTATGGACTATATCGGAGCGCTGGAGCGGGAGCTGGGAAAAACAGCCGAAAAAAATTTTCTGCCGATGCAGCCCG
GTGATGTTCCTGATACCTATGCCGATGTTGATCAGCTTATAGAGGATATCGGTTACAAGCCTGAAACGAGTGTCGATGAG
GGTATCAGGCGGTTTGTCGCCTGGTATCGGGAGTATTACGGCAGCAAGGAGTCCGGGAGCGTAAAGTAG

Upstream 100 bases:

>100_bases
TCGTTGCCGATGGAGGGATAGTTCTTTTTATGTATCTTCTTTTTTTTGGGAAGCTGAAGGATATGCCGAGGCTTTTTGCA
ACTTTAAATACCCAGCATCA

Downstream 100 bases:

>100_bases
CTGCTTTTTCCCTCGGTGCAGAGGTTCATGGTACCTGTTCTCACCAGTGAAACCAGTGTTACTGTTGCTTCATGGATCGT
AATGTCCGCAAGTATTCCAT

Product: NAD-dependent epimerase/dehydratase

Products: UDPglucoseal [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MNVLVTGAAGFIGFHVCRRLLDRGDSVTGLDNMNDYYDVHLKESRLAQLEPEEAFSFVKMDLADRAGMEELFDASRFDRV
INLAAQAGVRYSLINPYSYIDSNIQGFLNILEGCRHNGIEHLVYASSSSVYGANESMPFSVHDNVDHPLSLYAASKKANE
LMAHTYSHLYGIPSTGLRFFTVYGPWGRPDMALFLFTEAILAGRPIEVFNFGNHRRDFTYIDDIVEGVLRTLDHPAEPNP
DWTGLKPDPGTSRAPWRVYNIGNSRPVNLMDYIGALERELGKTAEKNFLPMQPGDVPDTYADVDQLIEDIGYKPETSVDE
GIRRFVAWYREYYGSKESGSVK

Sequences:

>Translated_342_residues
MNVLVTGAAGFIGFHVCRRLLDRGDSVTGLDNMNDYYDVHLKESRLAQLEPEEAFSFVKMDLADRAGMEELFDASRFDRV
INLAAQAGVRYSLINPYSYIDSNIQGFLNILEGCRHNGIEHLVYASSSSVYGANESMPFSVHDNVDHPLSLYAASKKANE
LMAHTYSHLYGIPSTGLRFFTVYGPWGRPDMALFLFTEAILAGRPIEVFNFGNHRRDFTYIDDIVEGVLRTLDHPAEPNP
DWTGLKPDPGTSRAPWRVYNIGNSRPVNLMDYIGALERELGKTAEKNFLPMQPGDVPDTYADVDQLIEDIGYKPETSVDE
GIRRFVAWYREYYGSKESGSVK
>Mature_342_residues
MNVLVTGAAGFIGFHVCRRLLDRGDSVTGLDNMNDYYDVHLKESRLAQLEPEEAFSFVKMDLADRAGMEELFDASRFDRV
INLAAQAGVRYSLINPYSYIDSNIQGFLNILEGCRHNGIEHLVYASSSSVYGANESMPFSVHDNVDHPLSLYAASKKANE
LMAHTYSHLYGIPSTGLRFFTVYGPWGRPDMALFLFTEAILAGRPIEVFNFGNHRRDFTYIDDIVEGVLRTLDHPAEPNP
DWTGLKPDPGTSRAPWRVYNIGNSRPVNLMDYIGALERELGKTAEKNFLPMQPGDVPDTYADVDQLIEDIGYKPETSVDE
GIRRFVAWYREYYGSKESGSVK

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=337, Percent_Identity=26.7062314540059, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI56237023, Length=352, Percent_Identity=25.5681818181818, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI56118217, Length=352, Percent_Identity=25.5681818181818, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI189083684, Length=352, Percent_Identity=25.5681818181818, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI42516563, Length=335, Percent_Identity=23.8805970149254, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1786974, Length=351, Percent_Identity=25.9259259259259, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1788353, Length=356, Percent_Identity=24.438202247191, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI48994969, Length=351, Percent_Identity=25.9259259259259, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71982035, Length=354, Percent_Identity=25.4237288135593, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI71982038, Length=356, Percent_Identity=25.2808988764045, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17568069, Length=344, Percent_Identity=24.1279069767442, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17539532, Length=345, Percent_Identity=24.9275362318841, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI115532424, Length=339, Percent_Identity=23.598820058997, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=359, Percent_Identity=25.0696378830084, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19923002, Length=351, Percent_Identity=25.9259259259259, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356223, Length=334, Percent_Identity=20.9580838323353, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 38505; Mature: 38505

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLVTGAAGFIGFHVCRRLLDRGDSVTGLDNMNDYYDVHLKESRLAQLEPEEAFSFVKM
CCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHHHHCCCHHHHHHHHHH
DLADRAGMEELFDASRFDRVINLAAQAGVRYSLINPYSYIDSNIQGFLNILEGCRHNGIE
HHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCC
HLVYASSSSVYGANESMPFSVHDNVDHPLSLYAASKKANELMAHTYSHLYGIPSTGLRFF
EEEEECCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
TVYGPWGRPDMALFLFTEAILAGRPIEVFNFGNHRRDFTYIDDIVEGVLRTLDHPAEPNP
EEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
DWTGLKPDPGTSRAPWRVYNIGNSRPVNLMDYIGALERELGKTAEKNFLPMQPGDVPDTY
CCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
ADVDQLIEDIGYKPETSVDEGIRRFVAWYREYYGSKESGSVK
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNVLVTGAAGFIGFHVCRRLLDRGDSVTGLDNMNDYYDVHLKESRLAQLEPEEAFSFVKM
CCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHHHHCCCHHHHHHHHHH
DLADRAGMEELFDASRFDRVINLAAQAGVRYSLINPYSYIDSNIQGFLNILEGCRHNGIE
HHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCC
HLVYASSSSVYGANESMPFSVHDNVDHPLSLYAASKKANELMAHTYSHLYGIPSTGLRFF
EEEEECCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
TVYGPWGRPDMALFLFTEAILAGRPIEVFNFGNHRRDFTYIDDIVEGVLRTLDHPAEPNP
EEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
DWTGLKPDPGTSRAPWRVYNIGNSRPVNLMDYIGALERELGKTAEKNFLPMQPGDVPDTY
CCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
ADVDQLIEDIGYKPETSVDEGIRRFVAWYREYYGSKESGSVK
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]