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The map label for this gene is metF [H]

Identifier: 119357520

GI number: 119357520

Start: 1951151

End: 1952020

Strand: Direct

Name: metF [H]

Synonym: Cpha266_1723

Alternate gene names: 119357520

Gene position: 1951151-1952020 (Clockwise)

Preceding gene: 119357516

Following gene: 119357522

Centisome position: 62.26

GC content: 50.0

Gene sequence:

>870_bases
ATGCTTGTCCGCGAGATCCTGAACTCCACCAGTGAACCTGTTTTCAGTTTTGAATTTTTTCCGCCTAAAAAAGAGGAGGA
GTGGAATACCCTTTTTGCAACCATTGCGAGCCTGACCTCTCTCAACCCCTCCTATGTGAGCGTTACCTACGGAGCCGGTG
GATCAACCCGTTCAAAAACGCATGACCTCGTCACAAGAATTCAGAAAGAGACCGGCATTACCGTTGTTTCCCATCTAACC
TGCGTCTGTTCGGACAGGGAGGAAACAAGCTCCATTCTTGAAAACTATCTTGAAAACGGCATCAACAACGTCCTTGCGCT
TCGCGGCGACAAGCCTCAGGGAGTAGCGACTCTTGCCGAAGCCATCAAGGATTTTGAGCATGCCGCAGACCTTATCCGGT
TTATCAAAATCCGATTCCCGGCAATCGGAATCGGTGTGGCAGGATTTCCTGAAGGTCATCCTGAAACACCAAACAGGCTT
AAGGAGATCGATTATCTCAAAGAGAAAATCGATGCGGGAGCAGACTATATCGTCACGCAGCTCTTTTTCGATAACAGGGA
TTATTTCGATTATGTTGAACGCTGCGCCATTGCCGGCATTACTGTACCGATCATTGCGGGTATCATGCCCGTTTCAACAA
AAAAAGGGATGATTCGCATGTCGGAGCTTGCCCTTGGCGCAAGAATTCCGGCCCCTCTCCTCAAGCAGGTTCTCGAAAGC
GACAGCGACAGGGATGTTGCTGAAATCGGCATTGACTGGGCCACCCGGCAGGTTCAGGAGCTGATCGACCAGCAGTGCAA
AGGCATCCACTTCTACACCCTGAACCTCGCCGACGCCACACTGAAAATCTTCAGGAACATCAGACAATAA

Upstream 100 bases:

>100_bases
GGTTATGGTAGAGCGCAGATAAATTTCTTTACCGTAACGAAATCATTGTTTTCATATATTCTTATCTTAAAAAAAGTAAC
GCAAAAAAACCACACATCTC

Downstream 100 bases:

>100_bases
ACACGCCTATCCGTAATCCGTTCAGAGCAGTTCGTTCTGGATAAGCTGCTCGTAGGTTTCGCGGGTGCGCACAAGCCGAA
CATCCTGGCCATCAACAAGA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MLVREILNSTSEPVFSFEFFPPKKEEEWNTLFATIASLTSLNPSYVSVTYGAGGSTRSKTHDLVTRIQKETGITVVSHLT
CVCSDREETSSILENYLENGINNVLALRGDKPQGVATLAEAIKDFEHAADLIRFIKIRFPAIGIGVAGFPEGHPETPNRL
KEIDYLKEKIDAGADYIVTQLFFDNRDYFDYVERCAIAGITVPIIAGIMPVSTKKGMIRMSELALGARIPAPLLKQVLES
DSDRDVAEIGIDWATRQVQELIDQQCKGIHFYTLNLADATLKIFRNIRQ

Sequences:

>Translated_289_residues
MLVREILNSTSEPVFSFEFFPPKKEEEWNTLFATIASLTSLNPSYVSVTYGAGGSTRSKTHDLVTRIQKETGITVVSHLT
CVCSDREETSSILENYLENGINNVLALRGDKPQGVATLAEAIKDFEHAADLIRFIKIRFPAIGIGVAGFPEGHPETPNRL
KEIDYLKEKIDAGADYIVTQLFFDNRDYFDYVERCAIAGITVPIIAGIMPVSTKKGMIRMSELALGARIPAPLLKQVLES
DSDRDVAEIGIDWATRQVQELIDQQCKGIHFYTLNLADATLKIFRNIRQ
>Mature_289_residues
MLVREILNSTSEPVFSFEFFPPKKEEEWNTLFATIASLTSLNPSYVSVTYGAGGSTRSKTHDLVTRIQKETGITVVSHLT
CVCSDREETSSILENYLENGINNVLALRGDKPQGVATLAEAIKDFEHAADLIRFIKIRFPAIGIGVAGFPEGHPETPNRL
KEIDYLKEKIDAGADYIVTQLFFDNRDYFDYVERCAIAGITVPIIAGIMPVSTKKGMIRMSELALGARIPAPLLKQVLES
DSDRDVAEIGIDWATRQVQELIDQQCKGIHFYTLNLADATLKIFRNIRQ

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=284, Percent_Identity=34.1549295774648, Blast_Score=152, Evalue=4e-37,
Organism=Escherichia coli, GI1790377, Length=269, Percent_Identity=38.6617100371747, Blast_Score=184, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI25147436, Length=291, Percent_Identity=30.5841924398625, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI25147441, Length=291, Percent_Identity=30.5841924398625, Blast_Score=135, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6325234, Length=300, Percent_Identity=36.3333333333333, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6321313, Length=286, Percent_Identity=32.8671328671329, Blast_Score=157, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24662658, Length=275, Percent_Identity=26.9090909090909, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 32215; Mature: 32215

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVREILNSTSEPVFSFEFFPPKKEEEWNTLFATIASLTSLNPSYVSVTYGAGGSTRSKT
CCHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHH
HDLVTRIQKETGITVVSHLTCVCSDREETSSILENYLENGINNVLALRGDKPQGVATLAE
HHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHH
AIKDFEHAADLIRFIKIRFPAIGIGVAGFPEGHPETPNRLKEIDYLKEKIDAGADYIVTQ
HHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
LFFDNRDYFDYVERCAIAGITVPIIAGIMPVSTKKGMIRMSELALGARIPAPLLKQVLES
HHHCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCC
DSDRDVAEIGIDWATRQVQELIDQQCKGIHFYTLNLADATLKIFRNIRQ
CCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MLVREILNSTSEPVFSFEFFPPKKEEEWNTLFATIASLTSLNPSYVSVTYGAGGSTRSKT
CCHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHH
HDLVTRIQKETGITVVSHLTCVCSDREETSSILENYLENGINNVLALRGDKPQGVATLAE
HHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHH
AIKDFEHAADLIRFIKIRFPAIGIGVAGFPEGHPETPNRLKEIDYLKEKIDAGADYIVTQ
HHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
LFFDNRDYFDYVERCAIAGITVPIIAGIMPVSTKKGMIRMSELALGARIPAPLLKQVLES
HHHCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCC
DSDRDVAEIGIDWATRQVQELIDQQCKGIHFYTLNLADATLKIFRNIRQ
CCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]