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The map label for this gene is panB

Identifier: 119357459

GI number: 119357459

Start: 1885910

End: 1886743

Strand: Reverse

Name: panB

Synonym: Cpha266_1658

Alternate gene names: 119357459

Gene position: 1886743-1885910 (Counterclockwise)

Preceding gene: 119357460

Following gene: 119357458

Centisome position: 60.2

GC content: 48.44

Gene sequence:

>834_bases
ATGAACACCAGTAACCAGCATAAAGCAGGACATGTTACTACAAGAAAGCTGCTGGATATGAAGCAGCAGGGAGAAAAAAT
ATCGGTATTGACTGCCTATGATTATACCATGGCAAGGATTCTTGATCGAGCCGGTATTGATGTTATTCTTGTGGGGGATT
CGGCAAGCAATGTTTTTTCCGGTCATGCCACTACGCTTCCCATTACGATTGAGGAGATGATCTATCACGCAAAGGCTGTT
GTCAGGGGTGTGCACGACGAGAGTGGGCGGGCGATGGTCGTCGTTGATATGCCGTTCATGAGCTATCAGATTTCAGGTGA
TGAGGCTTTGCGCAATGCGGGAAAGATCATGAAGGAGCATGGGTGTGATGCTCTCAAGCTGGAGGGAGGCAAGATCATTG
CCGATACCGTCAAGCGAATTACCGACGTCGGGATTCCGGTGATGGGTCATCTTGGTCTTATGCCGCAGTCCATCTATAAG
TATGGAAGCTACAAGGTCAGGGCTCAGGAGGAACAGGAGGCTGAACAGCTTCTTCAAGATGCCCGATTGCTTGAAGAGGC
GGGTGCTTTCGCTGTTGTTCTTGAAAAGATCCCCTCAGCGCTTGCTGCAGAGGTTACCCTTATGCTCACTATTCCGACTA
TCGGCATCGGAGCCGGATCGCATTGTGATGGACAGGTTCTGGTAGTCAATGATATTCTTGGTCTCAATCGTGAGTTTCAT
CCTCGTTTTGTTCGGCAGTACGCTAATTTAAATAACGTTATCGATCGTGCGGTACAACAGTATGTTGATGATGTCAAGCA
GGGAGGTTTTCCCGCTGATGATGAGAGTTACTGA

Upstream 100 bases:

>100_bases
CGGCTACATCGGGAGGCGCGTCGATGACGATGCTGGATCCTTTGCAGTTTGAGCGTTTTTTCAGAAATCAGGAGCCTTTT
ATTCATTAAGCGGTCAGAAT

Downstream 100 bases:

>100_bases
TGATTCTGATTTTTCTTTTTGTTAAATCGCTCTGAAAGAGTCTGCTGTTCAAAACGGTTTTTTTCAGCAGACTGCCATTT
GTGGTACGAAAAATCAAATC

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MNTSNQHKAGHVTTRKLLDMKQQGEKISVLTAYDYTMARILDRAGIDVILVGDSASNVFSGHATTLPITIEEMIYHAKAV
VRGVHDESGRAMVVVDMPFMSYQISGDEALRNAGKIMKEHGCDALKLEGGKIIADTVKRITDVGIPVMGHLGLMPQSIYK
YGSYKVRAQEEQEAEQLLQDARLLEEAGAFAVVLEKIPSALAAEVTLMLTIPTIGIGAGSHCDGQVLVVNDILGLNREFH
PRFVRQYANLNNVIDRAVQQYVDDVKQGGFPADDESY

Sequences:

>Translated_277_residues
MNTSNQHKAGHVTTRKLLDMKQQGEKISVLTAYDYTMARILDRAGIDVILVGDSASNVFSGHATTLPITIEEMIYHAKAV
VRGVHDESGRAMVVVDMPFMSYQISGDEALRNAGKIMKEHGCDALKLEGGKIIADTVKRITDVGIPVMGHLGLMPQSIYK
YGSYKVRAQEEQEAEQLLQDARLLEEAGAFAVVLEKIPSALAAEVTLMLTIPTIGIGAGSHCDGQVLVVNDILGLNREFH
PRFVRQYANLNNVIDRAVQQYVDDVKQGGFPADDESY
>Mature_277_residues
MNTSNQHKAGHVTTRKLLDMKQQGEKISVLTAYDYTMARILDRAGIDVILVGDSASNVFSGHATTLPITIEEMIYHAKAV
VRGVHDESGRAMVVVDMPFMSYQISGDEALRNAGKIMKEHGCDALKLEGGKIIADTVKRITDVGIPVMGHLGLMPQSIYK
YGSYKVRAQEEQEAEQLLQDARLLEEAGAFAVVLEKIPSALAAEVTLMLTIPTIGIGAGSHCDGQVLVVNDILGLNREFH
PRFVRQYANLNNVIDRAVQQYVDDVKQGGFPADDESY

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=266, Percent_Identity=39.8496240601504, Blast_Score=207, Evalue=9e-55,
Organism=Saccharomyces cerevisiae, GI6319653, Length=255, Percent_Identity=34.5098039215686, Blast_Score=174, Evalue=2e-44,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_CHLPD (A1BH02)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_912103.1
- ProteinModelPortal:   A1BH02
- SMR:   A1BH02
- STRING:   A1BH02
- GeneID:   4571038
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_1658
- NMPDR:   fig|290317.7.peg.1740
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 30328; Mature: 30328

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: ACT_SITE 195-195 BINDING 96-96 BINDING 126-126

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSNQHKAGHVTTRKLLDMKQQGEKISVLTAYDYTMARILDRAGIDVILVGDSASNVFS
CCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHCCCEEEEECCCCCCCCC
GHATTLPITIEEMIYHAKAVVRGVHDESGRAMVVVDMPFMSYQISGDEALRNAGKIMKEH
CCCEEECEEHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCHHHHHHHHHHHHHC
GCDALKLEGGKIIADTVKRITDVGIPVMGHLGLMPQSIYKYGSYKVRAQEEQEAEQLLQD
CCCEEEECCCEEHHHHHHHHHHCCCCHHHHHCCCHHHHHHCCCEEEECCHHHHHHHHHHH
ARLLEEAGAFAVVLEKIPSALAAEVTLMLTIPTIGIGAGSHCDGQVLVVNDILGLNREFH
HHHHHHCCHHHHHHHHHHHHHHHHEEEEEEECEEECCCCCCCCCEEEEEECHHCCCCCCC
PRFVRQYANLNNVIDRAVQQYVDDVKQGGFPADDESY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNTSNQHKAGHVTTRKLLDMKQQGEKISVLTAYDYTMARILDRAGIDVILVGDSASNVFS
CCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHCCCEEEEECCCCCCCCC
GHATTLPITIEEMIYHAKAVVRGVHDESGRAMVVVDMPFMSYQISGDEALRNAGKIMKEH
CCCEEECEEHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCHHHHHHHHHHHHHC
GCDALKLEGGKIIADTVKRITDVGIPVMGHLGLMPQSIYKYGSYKVRAQEEQEAEQLLQD
CCCEEEECCCEEHHHHHHHHHHCCCCHHHHHCCCHHHHHHCCCEEEECCHHHHHHHHHHH
ARLLEEAGAFAVVLEKIPSALAAEVTLMLTIPTIGIGAGSHCDGQVLVVNDILGLNREFH
HHHHHHCCHHHHHHHHHHHHHHHHEEEEEEECEEECCCCCCCCCEEEEEECHHCCCCCCC
PRFVRQYANLNNVIDRAVQQYVDDVKQGGFPADDESY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA