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The map label for this gene is pvuIIM [H]

Identifier: 119357320

GI number: 119357320

Start: 1725647

End: 1726501

Strand: Reverse

Name: pvuIIM [H]

Synonym: Cpha266_1518

Alternate gene names: 119357320

Gene position: 1726501-1725647 (Counterclockwise)

Preceding gene: 119357322

Following gene: 119357319

Centisome position: 55.09

GC content: 44.44

Gene sequence:

>855_bases
ATGGAAATAAGAACGGATCTATATTTGGGGGATTGCAGGGAGATTCTTGAAAACCTGCCGGATGAATCAGTGGATCTGAT
ATTTACCTCTCCACCATATGCCGATCAACGGAAACAAACCTATGGCGGGTTCCATCCGGACGACTATGTCGCATGGTTTT
TACCAATATCCTTTCAGCTCATGAGAGTTCTCAAGCCCACGGGAACGTTTGTTTTGAACATCAAGGAAAAGGTGCTTAAC
GGTGAACGGAGTACCTATGTGATGGAACTTATTCTTGAAATGAGAAAACAACAGGGATGGCTCTGGACTGAAGAGTTCAT
CTGGCACAAAAAAAACTGTTATCCGGGAAAATGGCCAAACAGGTTCAGGGACTCCTGGGAGCGTCTCATCCAGTTCAATA
AGCGCAAACAGTTCTACATGAATCAGGATGCCGTAATGGTGCCAATGGGGGATTGGTCGAGAACCCGTCTGAAAAATCTG
AGTGAAACAGACAAGATGCGTGATGAATCAAAGGTCGGCAGCGGGTTTGGAAAAAACATCTCAAACTGGCTCCAGAGGGA
TATGGCATATCCGACCAATGTGCTGCACCTTGCAACAGAGTGCAATAACAAAAATCACAGCGCAGCGTTTCCTGAAGGGC
TCCCTGAATGGTTTATCAAACTGTTCACCAAAGAGGGCGATACTGTTCTCGATCCGTTCATGGGTTCAGGAACAACCAAC
GCGGTTGCAAAAAGAATGAAAAGAAACTCCATTGGCATTGAAATTATGACGGAGTATTATGAGATGGTTAAAAATGAACT
GAAACCGTCAGAACTCTATCTCCTTGAACAACAAAAAGGATATGACACCAATTGA

Upstream 100 bases:

>100_bases
GGGAAAATATGTAAGTTGGCTATAAGTCCGGATGGGACAACTCCGCGCCATTCATGCTGACAGGAAATCGATACGTGAAA
ATTATTGCACTTTACAAGAA

Downstream 100 bases:

>100_bases
CCTGACCGACGTAACGAAATACGTTGAAACACATATTGGCGGCTTTCATCAGAAAAGAATAGCAAGTCTTGATGGATTAA
GCCTTATAAAGGTATTGAAA

Product: DNA methylase N-4/N-6 domain-containing protein

Products: NA

Alternate protein names: M.PvuII; N-4 cytosine-specific methyltransferase PvuII [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MEIRTDLYLGDCREILENLPDESVDLIFTSPPYADQRKQTYGGFHPDDYVAWFLPISFQLMRVLKPTGTFVLNIKEKVLN
GERSTYVMELILEMRKQQGWLWTEEFIWHKKNCYPGKWPNRFRDSWERLIQFNKRKQFYMNQDAVMVPMGDWSRTRLKNL
SETDKMRDESKVGSGFGKNISNWLQRDMAYPTNVLHLATECNNKNHSAAFPEGLPEWFIKLFTKEGDTVLDPFMGSGTTN
AVAKRMKRNSIGIEIMTEYYEMVKNELKPSELYLLEQQKGYDTN

Sequences:

>Translated_284_residues
MEIRTDLYLGDCREILENLPDESVDLIFTSPPYADQRKQTYGGFHPDDYVAWFLPISFQLMRVLKPTGTFVLNIKEKVLN
GERSTYVMELILEMRKQQGWLWTEEFIWHKKNCYPGKWPNRFRDSWERLIQFNKRKQFYMNQDAVMVPMGDWSRTRLKNL
SETDKMRDESKVGSGFGKNISNWLQRDMAYPTNVLHLATECNNKNHSAAFPEGLPEWFIKLFTKEGDTVLDPFMGSGTTN
AVAKRMKRNSIGIEIMTEYYEMVKNELKPSELYLLEQQKGYDTN
>Mature_284_residues
MEIRTDLYLGDCREILENLPDESVDLIFTSPPYADQRKQTYGGFHPDDYVAWFLPISFQLMRVLKPTGTFVLNIKEKVLN
GERSTYVMELILEMRKQQGWLWTEEFIWHKKNCYPGKWPNRFRDSWERLIQFNKRKQFYMNQDAVMVPMGDWSRTRLKNL
SETDKMRDESKVGSGFGKNISNWLQRDMAYPTNVLHLATECNNKNHSAAFPEGLPEWFIKLFTKEGDTVLDPFMGSGTTN
AVAKRMKRNSIGIEIMTEYYEMVKNELKPSELYLLEQQKGYDTN

Specific function: This methylase recognizes the double-stranded sequence CAGCTG, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the PvuII endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=277, Percent_Identity=26.3537906137184, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 33479; Mature: 33479

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIRTDLYLGDCREILENLPDESVDLIFTSPPYADQRKQTYGGFHPDDYVAWFLPISFQL
CCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHH
MRVLKPTGTFVLNIKEKVLNGERSTYVMELILEMRKQQGWLWTEEFIWHKKNCYPGKWPN
HHHHCCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHCCCCCCCCCCH
RFRDSWERLIQFNKRKQFYMNQDAVMVPMGDWSRTRLKNLSETDKMRDESKVGSGFGKNI
HHHHHHHHHHHHHHHHHEEECCCEEEEECCCCHHHHHHCCHHHHHHHHHHHHCCCCCCHH
SNWLQRDMAYPTNVLHLATECNNKNHSAAFPEGLPEWFIKLFTKEGDTVLDPFMGSGTTN
HHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCHH
AVAKRMKRNSIGIEIMTEYYEMVKNELKPSELYLLEQQKGYDTN
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MEIRTDLYLGDCREILENLPDESVDLIFTSPPYADQRKQTYGGFHPDDYVAWFLPISFQL
CCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHH
MRVLKPTGTFVLNIKEKVLNGERSTYVMELILEMRKQQGWLWTEEFIWHKKNCYPGKWPN
HHHHCCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHCCCCCCCCCCH
RFRDSWERLIQFNKRKQFYMNQDAVMVPMGDWSRTRLKNLSETDKMRDESKVGSGFGKNI
HHHHHHHHHHHHHHHHHEEECCCEEEEECCCCHHHHHHCCHHHHHHHHHHHHCCCCCCHH
SNWLQRDMAYPTNVLHLATECNNKNHSAAFPEGLPEWFIKLFTKEGDTVLDPFMGSGTTN
HHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCHH
AVAKRMKRNSIGIEIMTEYYEMVKNELKPSELYLLEQQKGYDTN
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2662138; 7607491; 9207015 [H]