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The map label for this gene is hsdS [H]

Identifier: 119357296

GI number: 119357296

Start: 1692656

End: 1694095

Strand: Reverse

Name: hsdS [H]

Synonym: Cpha266_1492

Alternate gene names: 119357296

Gene position: 1694095-1692656 (Counterclockwise)

Preceding gene: 119357297

Following gene: 119357295

Centisome position: 54.06

GC content: 41.6

Gene sequence:

>1440_bases
ATGACAAGTAATCATCATGTGATTGCAATATTAGGAGATGTCGCTGAATATATAAATGGTCGTGCGTTTAAACCGTCAGA
GTGGGGAAAAGAAGGTCTCCCTATCATTAGGATAAAAAATTTGAATGATGAAAACTCAAAATTCAATTATAGTAATGAGG
TTTTTGAAAAAAGGTACCTTGTGAAAAAAGGGGATTTACTTTTTGCTTGGTCTGCCTCTCTCGGTGCATACATATGGAAA
AAAGATGAAGCTTGGTTAAATCAACATATTTTTCTTGTCAAACCGAGTCCGTTTATAGCAAAACTATACCTTTATTATTT
TCTCGACAAAATAACACAAGAGCTTTATTCTGCTGCACATGGTTCCGGAATGGTCCATGTTACAAAGAAGAAATTTGAGG
AAACTAAGATTGGTTTACCGCCACTATCTGAGCAACGATCCATCGTTTCCAAAATCGAGCAGCTTTTCAGCGAACTTGAT
AACGGGATTGCCTGTCTGAAAAAAGCACAGGAGCAACTTAAAGTCTATCGTCAGGCTGTTCTGAAGCAAGCGTTTGAGGG
TGAACTCACAAAATCCTGGCGCGAACAGCAAGCCAACCTCCCGTCAGCACAGGATCTTCTCGATACGATCAAGACAGAAC
GAGAGCAAGCTGCAAAAAATCAGGGTAAAAAGCTCAAGCCGGTAACTCCTCTTGCAAAAGTGGAACTTGATGAGTTGACT
GAACTGCCGGATGGGTGGTGCTGGATAAAATTAGGTGAGTTGACCATCGGTGTTGAGTATGGGACTTCAACAAAATCACT
TGAAAAAGGTGAGGTTCCCGTAATAAGAATGGGCAATATTCAGCAAGGTCGAATTGATTGGAATGATTTGGCTTTTACCG
ATGATAAGGCGGATATTTCAAAATATCGATTGTTAAAAGGTGATGTCCTTTTTAATAGGACAAATAGCCCGGAACTCGTT
GGTAAAGCCGCGATCTATAATGGAGAAATGCCTGCTATTTTTGCAGGATACCTCATCAGAGTCAATCAAATCAAAGAATT
ATTGCACTGCAAGTATCTCAACTTTTTCCTGAATTCTCATCCTGCAAAAGTTTATGGCAATTCAGTAAAGACTGATGGAG
TAAATCAGTCAAACATCAATGGGGAAAAACTCAAAAGTTATCCCTTGCCATATTGTTCACCAAAAGAGCAAGAGCAAATC
GTGCAGGAAATTGAGGCGCGCCTTTCGGTTTGCGACAACATGGAGGCAACAATCCGCGAATCGCTTGAAAAAGCTGAGGC
CTTACGGCAAAGCATTCTGAAAAAAGCATTCGAGGGCAAGTTGCTCAGCGAGGAGGAGTTAACGGCAACCCGCAACGATC
CGGACTGGGAGCCTGCCGAGAAGCTGCTTGAGCGGATCAGGGCTGAAAAAAACCAATCGAAGAAACAAGCATTAACGTAG

Upstream 100 bases:

>100_bases
AAAAAGAAAGCCGGTCGTATGCTCGATGGGCGCGTTTGGGATCAGACACCGGAAATGCCGGGATTGTTGGTTGGTTAAAT
GATACAAAGAGTGTAGGAAA

Downstream 100 bases:

>100_bases
TACCCATGACCAACACCCCATCATCCATAGTTTCGAAAGTCTGGTCGTTCTGCCACGTGCTGCGCGACAGCGGCGTGAGC
TATGGCGACTATCTCGAACA

Product: restriction modification system DNA specificity subunit

Products: NA

Alternate protein names: S.EcoKI; Type I restriction enzyme EcoKI specificity protein; S protein [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTSNHHVIAILGDVAEYINGRAFKPSEWGKEGLPIIRIKNLNDENSKFNYSNEVFEKRYLVKKGDLLFAWSASLGAYIWK
KDEAWLNQHIFLVKPSPFIAKLYLYYFLDKITQELYSAAHGSGMVHVTKKKFEETKIGLPPLSEQRSIVSKIEQLFSELD
NGIACLKKAQEQLKVYRQAVLKQAFEGELTKSWREQQANLPSAQDLLDTIKTEREQAAKNQGKKLKPVTPLAKVELDELT
ELPDGWCWIKLGELTIGVEYGTSTKSLEKGEVPVIRMGNIQQGRIDWNDLAFTDDKADISKYRLLKGDVLFNRTNSPELV
GKAAIYNGEMPAIFAGYLIRVNQIKELLHCKYLNFFLNSHPAKVYGNSVKTDGVNQSNINGEKLKSYPLPYCSPKEQEQI
VQEIEARLSVCDNMEATIRESLEKAEALRQSILKKAFEGKLLSEEELTATRNDPDWEPAEKLLERIRAEKNQSKKQALT

Sequences:

>Translated_479_residues
MTSNHHVIAILGDVAEYINGRAFKPSEWGKEGLPIIRIKNLNDENSKFNYSNEVFEKRYLVKKGDLLFAWSASLGAYIWK
KDEAWLNQHIFLVKPSPFIAKLYLYYFLDKITQELYSAAHGSGMVHVTKKKFEETKIGLPPLSEQRSIVSKIEQLFSELD
NGIACLKKAQEQLKVYRQAVLKQAFEGELTKSWREQQANLPSAQDLLDTIKTEREQAAKNQGKKLKPVTPLAKVELDELT
ELPDGWCWIKLGELTIGVEYGTSTKSLEKGEVPVIRMGNIQQGRIDWNDLAFTDDKADISKYRLLKGDVLFNRTNSPELV
GKAAIYNGEMPAIFAGYLIRVNQIKELLHCKYLNFFLNSHPAKVYGNSVKTDGVNQSNINGEKLKSYPLPYCSPKEQEQI
VQEIEARLSVCDNMEATIRESLEKAEALRQSILKKAFEGKLLSEEELTATRNDPDWEPAEKLLERIRAEKNQSKKQALT
>Mature_478_residues
TSNHHVIAILGDVAEYINGRAFKPSEWGKEGLPIIRIKNLNDENSKFNYSNEVFEKRYLVKKGDLLFAWSASLGAYIWKK
DEAWLNQHIFLVKPSPFIAKLYLYYFLDKITQELYSAAHGSGMVHVTKKKFEETKIGLPPLSEQRSIVSKIEQLFSELDN
GIACLKKAQEQLKVYRQAVLKQAFEGELTKSWREQQANLPSAQDLLDTIKTEREQAAKNQGKKLKPVTPLAKVELDELTE
LPDGWCWIKLGELTIGVEYGTSTKSLEKGEVPVIRMGNIQQGRIDWNDLAFTDDKADISKYRLLKGDVLFNRTNSPELVG
KAAIYNGEMPAIFAGYLIRVNQIKELLHCKYLNFFLNSHPAKVYGNSVKTDGVNQSNINGEKLKSYPLPYCSPKEQEQIV
QEIEARLSVCDNMEATIRESLEKAEALRQSILKKAFEGKLLSEEELTATRNDPDWEPAEKLLERIRAEKNQSKKQALT

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

Organism=Escherichia coli, GI1790807, Length=497, Percent_Identity=26.9617706237425, Blast_Score=135, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 54548; Mature: 54417

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSNHHVIAILGDVAEYINGRAFKPSEWGKEGLPIIRIKNLNDENSKFNYSNEVFEKRYL
CCCCCEEEEEHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHH
VKKGDLLFAWSASLGAYIWKKDEAWLNQHIFLVKPSPFIAKLYLYYFLDKITQELYSAAH
HCCCCEEEEECCCCCCEEEECCHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHC
GSGMVHVTKKKFEETKIGLPPLSEQRSIVSKIEQLFSELDNGIACLKKAQEQLKVYRQAV
CCCEEEEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKQAFEGELTKSWREQQANLPSAQDLLDTIKTEREQAAKNQGKKLKPVTPLAKVELDELT
HHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHH
ELPDGWCWIKLGELTIGVEYGTSTKSLEKGEVPVIRMGNIQQGRIDWNDLAFTDDKADIS
CCCCCEEEEEECCEEEEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCEECCCCHHHH
KYRLLKGDVLFNRTNSPELVGKAAIYNGEMPAIFAGYLIRVNQIKELLHCKYLNFFLNSH
HHHHEECCEEEECCCCCHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PAKVYGNSVKTDGVNQSNINGEKLKSYPLPYCSPKEQEQIVQEIEARLSVCDNMEATIRE
CHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHH
SLEKAEALRQSILKKAFEGKLLSEEELTATRNDPDWEPAEKLLERIRAEKNQSKKQALT
HHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHCCC
>Mature Secondary Structure 
TSNHHVIAILGDVAEYINGRAFKPSEWGKEGLPIIRIKNLNDENSKFNYSNEVFEKRYL
CCCCEEEEEHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHH
VKKGDLLFAWSASLGAYIWKKDEAWLNQHIFLVKPSPFIAKLYLYYFLDKITQELYSAAH
HCCCCEEEEECCCCCCEEEECCHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHC
GSGMVHVTKKKFEETKIGLPPLSEQRSIVSKIEQLFSELDNGIACLKKAQEQLKVYRQAV
CCCEEEEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKQAFEGELTKSWREQQANLPSAQDLLDTIKTEREQAAKNQGKKLKPVTPLAKVELDELT
HHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHH
ELPDGWCWIKLGELTIGVEYGTSTKSLEKGEVPVIRMGNIQQGRIDWNDLAFTDDKADIS
CCCCCEEEEEECCEEEEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCEECCCCHHHH
KYRLLKGDVLFNRTNSPELVGKAAIYNGEMPAIFAGYLIRVNQIKELLHCKYLNFFLNSH
HHHHEECCEEEECCCCCHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PAKVYGNSVKTDGVNQSNINGEKLKSYPLPYCSPKEQEQIVQEIEARLSVCDNMEATIRE
CHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHH
SLEKAEALRQSILKKAFEGKLLSEEELTATRNDPDWEPAEKLLERIRAEKNQSKKQALT
HHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6304321; 7610040; 9278503; 7739896 [H]