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The map label for this gene is dacB [H]

Identifier: 119357228

GI number: 119357228

Start: 1613643

End: 1614518

Strand: Reverse

Name: dacB [H]

Synonym: Cpha266_1420

Alternate gene names: 119357228

Gene position: 1614518-1613643 (Counterclockwise)

Preceding gene: 119357229

Following gene: 119357227

Centisome position: 51.52

GC content: 45.78

Gene sequence:

>876_bases
ATGAACAGAAAAGACCGGATGTTCAGGAAAAGCGTTCTGTTTTTATTCTCCATGTTTCTGGCATCTGGTATTTCCATGCC
TGTTTACGCAGCGTTGCCGGTTGATTCCTTGTTGCAACCTCTTCAGCCGCCTGAAATTGGTTCCTATATTCTCAAGGAGA
CGGGAAGTTCAAAGGTTTTTATGGTCAAGGACAGTGAAAAAACCATACAGCCTGCAAGTTTGACGAAAATTCTCACCAGT
ATTATTGCGCTTGAGAGCGGTCAACTTGATGATGAGGTGGTCATAACAAAGGAGGCGACGATGGTTGAGCCTTCAAAAGC
CGGGTTCAAGCCGGGTGAGCGGATCTTGCTGAGAGATCTGGTTAAAGCCGCGATGGTTAGCTCAAGCAACGATGCGGCTT
TTGCTATTGGTATTCACCTTGAGGGGAGCGTTCCCCGTTTTGTCAGAAAAATGAACCGCAAGGCTCGGGATATCGGGATG
CGGCATTCGGTTTTTACCAATCCTGCCGGTTTCGACAAACGTGTTTATGCCGGTAATGTTTCGACTGCCGAGGATCTTGT
GAAGCTTACTGAGTATGCGATTAGAAATCGTAAGTTTAACGAGATAGCAAGTCTTGAATCTGTTCTTGTAACTGAACGGA
GCAGTAATAAAATCTACCTGCTTAAAACCCACAACAAGTTACTTGGCAAATACCCGTATGCGGTAGGAATAAAGACCGGA
TTTACCCGAAGTGCCGGCAAGTGCCTTATTGCAAGGGCAAAAAAGGAAAATAAGGATATGTTGCTCGTATTGCTCGATGC
CAAGGGCGATCGCTGGAGCATAGCTGAACAGATGTTTGAATCGGCGTTTACTCCTCCGTTTCAGGCTTATCAATAA

Upstream 100 bases:

>100_bases
TGATGTTTCGATTTGTTTTTTACCAAACATGAATTCTCCTATGATAACGTACATTTCGCATGGTAATTCCGGTTCCGTGA
GGAGCCGTTTCGGTGCTTTC

Downstream 100 bases:

>100_bases
TGCCTGTTGGGGCGACGCATGGTTATGATCTTTTTTCAAATTTTCGGGACAGGAAGGCAGAGCGAGCGGGTGTAGGGGCG
AAGGGTTTGGTTGTTGCGTT

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MNRKDRMFRKSVLFLFSMFLASGISMPVYAALPVDSLLQPLQPPEIGSYILKETGSSKVFMVKDSEKTIQPASLTKILTS
IIALESGQLDDEVVITKEATMVEPSKAGFKPGERILLRDLVKAAMVSSSNDAAFAIGIHLEGSVPRFVRKMNRKARDIGM
RHSVFTNPAGFDKRVYAGNVSTAEDLVKLTEYAIRNRKFNEIASLESVLVTERSSNKIYLLKTHNKLLGKYPYAVGIKTG
FTRSAGKCLIARAKKENKDMLLVLLDAKGDRWSIAEQMFESAFTPPFQAYQ

Sequences:

>Translated_291_residues
MNRKDRMFRKSVLFLFSMFLASGISMPVYAALPVDSLLQPLQPPEIGSYILKETGSSKVFMVKDSEKTIQPASLTKILTS
IIALESGQLDDEVVITKEATMVEPSKAGFKPGERILLRDLVKAAMVSSSNDAAFAIGIHLEGSVPRFVRKMNRKARDIGM
RHSVFTNPAGFDKRVYAGNVSTAEDLVKLTEYAIRNRKFNEIASLESVLVTERSSNKIYLLKTHNKLLGKYPYAVGIKTG
FTRSAGKCLIARAKKENKDMLLVLLDAKGDRWSIAEQMFESAFTPPFQAYQ
>Mature_291_residues
MNRKDRMFRKSVLFLFSMFLASGISMPVYAALPVDSLLQPLQPPEIGSYILKETGSSKVFMVKDSEKTIQPASLTKILTS
IIALESGQLDDEVVITKEATMVEPSKAGFKPGERILLRDLVKAAMVSSSNDAAFAIGIHLEGSVPRFVRKMNRKARDIGM
RHSVFTNPAGFDKRVYAGNVSTAEDLVKLTEYAIRNRKFNEIASLESVLVTERSSNKIYLLKTHNKLLGKYPYAVGIKTG
FTRSAGKCLIARAKKENKDMLLVLLDAKGDRWSIAEQMFESAFTPPFQAYQ

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=258, Percent_Identity=31.3953488372093, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082022, Length=281, Percent_Identity=28.8256227758007, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI87082054, Length=244, Percent_Identity=29.9180327868852, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786851, Length=217, Percent_Identity=30.4147465437788, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 32396; Mature: 32396

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRKDRMFRKSVLFLFSMFLASGISMPVYAALPVDSLLQPLQPPEIGSYILKETGSSKVF
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHCCCCCCCHHHHHHHCCCCCEEE
MVKDSEKTIQPASLTKILTSIIALESGQLDDEVVITKEATMVEPSKAGFKPGERILLRDL
EEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEECCCCCCCCCCHHHHHHHH
VKAAMVSSSNDAAFAIGIHLEGSVPRFVRKMNRKARDIGMRHSVFTNPAGFDKRVYAGNV
HHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCHHHEECCCCCCCCEEEECCC
STAEDLVKLTEYAIRNRKFNEIASLESVLVTERSSNKIYLLKTHNKLLGKYPYAVGIKTG
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCEEEEEECCCHHHHCCCEEEEECCC
FTRSAGKCLIARAKKENKDMLLVLLDAKGDRWSIAEQMFESAFTPPFQAYQ
CCCCCCCEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNRKDRMFRKSVLFLFSMFLASGISMPVYAALPVDSLLQPLQPPEIGSYILKETGSSKVF
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHCCCCCCCHHHHHHHCCCCCEEE
MVKDSEKTIQPASLTKILTSIIALESGQLDDEVVITKEATMVEPSKAGFKPGERILLRDL
EEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEECCCCCCCCCCHHHHHHHH
VKAAMVSSSNDAAFAIGIHLEGSVPRFVRKMNRKARDIGMRHSVFTNPAGFDKRVYAGNV
HHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCHHHEECCCCCCCCEEEECCC
STAEDLVKLTEYAIRNRKFNEIASLESVLVTERSSNKIYLLKTHNKLLGKYPYAVGIKTG
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCEEEEEECCCHHHHCCCEEEEECCC
FTRSAGKCLIARAKKENKDMLLVLLDAKGDRWSIAEQMFESAFTPPFQAYQ
CCCCCCCEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]