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The map label for this gene is rlmB [H]

Identifier: 119357220

GI number: 119357220

Start: 1607342

End: 1607884

Strand: Reverse

Name: rlmB [H]

Synonym: Cpha266_1411

Alternate gene names: 119357220

Gene position: 1607884-1607342 (Counterclockwise)

Preceding gene: 119357221

Following gene: 119357219

Centisome position: 51.31

GC content: 51.57

Gene sequence:

>543_bases
ATGAGCCAGTTTAGAAAGCTGTCAGGACTGGAGATGAACCGGTTGGACGCGAAGAGCGTTTTTGATGTACCAAGGCATCC
GGTTACTCTTATGCTCGATAATATCCGAAGCATGTGGAACGTGGGGGCAATGTTCAGAACAGCGGATGCTGCGGGCATTG
AAAAGATCATTCTGACCGGCTATACGGCAGCGCCTCCGAGAAAAGAGATCGATAAAACCGCCCTTGGCGCCCAGGAAACG
GTGCGTTGGGAGTATTGTGAGAGCGCTCTTGAGGCCATAGCAACGCTTAAAGCGCGCGGCGTGAAAATCTGCGCACTTGA
AATAGCCGAGCCGAGCCGCCCCTATACGGCAATGCGAAAGACGGATTTTCCTCTTTGTCTTATTGTTGGCAATGAGGTTG
ATGGTATCGGGGACGAGCTGCTTCGAGAGTGTGACGAGGTGATTGAAATTCCGCAGTATGGCGCAAAACATTCTCTGAAT
GTCGCAGTTGCTGCTGGCGTTTCGCTTTTCGAGTTTATCAGGGTGATTCGCGCAACCCTGTAA

Upstream 100 bases:

>100_bases
GATGTATCCGGATATCCTCTTATGGAGAACTGTCTTCGTTTCAATATCGGGCTCAGGGATGAAAACGATGCTTTGCTTCA
GCAACTCAGGCTTTTATGCG

Downstream 100 bases:

>100_bases
CTTTTTGTTCGCGCGGCTTGTTTATACATCATATAGTTTAACAGCAATTAAAAAAGTGTTCTTCAATGCTTCACTATAAA
ACATATGAACTGGCCGATAC

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MSQFRKLSGLEMNRLDAKSVFDVPRHPVTLMLDNIRSMWNVGAMFRTADAAGIEKIILTGYTAAPPRKEIDKTALGAQET
VRWEYCESALEAIATLKARGVKICALEIAEPSRPYTAMRKTDFPLCLIVGNEVDGIGDELLRECDEVIEIPQYGAKHSLN
VAVAAGVSLFEFIRVIRATL

Sequences:

>Translated_180_residues
MSQFRKLSGLEMNRLDAKSVFDVPRHPVTLMLDNIRSMWNVGAMFRTADAAGIEKIILTGYTAAPPRKEIDKTALGAQET
VRWEYCESALEAIATLKARGVKICALEIAEPSRPYTAMRKTDFPLCLIVGNEVDGIGDELLRECDEVIEIPQYGAKHSLN
VAVAAGVSLFEFIRVIRATL
>Mature_179_residues
SQFRKLSGLEMNRLDAKSVFDVPRHPVTLMLDNIRSMWNVGAMFRTADAAGIEKIILTGYTAAPPRKEIDKTALGAQETV
RWEYCESALEAIATLKARGVKICALEIAEPSRPYTAMRKTDFPLCLIVGNEVDGIGDELLRECDEVIEIPQYGAKHSLNV
AVAAGVSLFEFIRVIRATL

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=149, Percent_Identity=32.2147651006711, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI1790083, Length=155, Percent_Identity=30.3225806451613, Blast_Score=67, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 19913; Mature: 19782

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQFRKLSGLEMNRLDAKSVFDVPRHPVTLMLDNIRSMWNVGAMFRTADAAGIEKIILTG
CCCHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEEC
YTAAPPRKEIDKTALGAQETVRWEYCESALEAIATLKARGVKICALEIAEPSRPYTAMRK
CCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCHHHHC
TDFPLCLIVGNEVDGIGDELLRECDEVIEIPQYGAKHSLNVAVAAGVSLFEFIRVIRATL
CCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQFRKLSGLEMNRLDAKSVFDVPRHPVTLMLDNIRSMWNVGAMFRTADAAGIEKIILTG
CCHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEEC
YTAAPPRKEIDKTALGAQETVRWEYCESALEAIATLKARGVKICALEIAEPSRPYTAMRK
CCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCHHHHC
TDFPLCLIVGNEVDGIGDELLRECDEVIEIPQYGAKHSLNVAVAAGVSLFEFIRVIRATL
CCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]