Click here to switch to the map view.

The map label for this gene is nifD [H]

Identifier: 119356574

GI number: 119356574

Start: 842766

End: 844406

Strand: Reverse

Name: nifD [H]

Synonym: Cpha266_0741

Alternate gene names: 119356574

Gene position: 844406-842766 (Counterclockwise)

Preceding gene: 119356575

Following gene: 119356573

Centisome position: 26.94

GC content: 49.73

Gene sequence:

>1641_bases
ATGCAGTCGTACAAAAATTTACCGGATCCATCCCTGGTCAGGGAGGATCTGATACAGAAATACCCGACCAAAGTTGCAAA
AAAACGCAACAAGTCAATTGTAATCAATGACCCGGAGACCATTCCGGAGGTGCAGGCAAACGTACGCACCGTTCCGGGAA
TCATTACCCAGCGCGGCTGCTGCTATGCCGGTTGTAAAGGTGTTGTTCTTGGACCGACGCGTGATATCGTCAACATTGTT
CACGGACCTATCGGGTGCAGCTTCTATGCCTGGTTGACCCGTCGGAACCAGACGAGACCGGAAACACCGGAAGCCGAGAA
CTATATCACCTACTGTTTCTCAACCGACATGCAGGAGGAGAACGTTGTGTTCGGCGGCGAAAAGAAACTGAAGCAGGCCA
TACAGGAAGCCTATGATCTCTTTCATCCGAAGGCTATCGCTATCTTTTCGACCTGTCCGGTTGGTCTTATTGGCGACGAT
GTGCATGCCGCATCAAAAGAGATGCGTGATAAATTCGGCGACTGTAACGTTTTCGGGTTCAGTTGTGAAGGGTACCGGGG
TGTCAGCCAGTCGGCAGGCCACCATATTGCCAACAACGGCGTTTTCAAACACATGGTAGGACGCAACAACGCAGTCAAAG
AGGGAAAGTTCAAATTAAACCTGCTTGGTGAATACAATATTGGCGGTGATGCGTTTGAAATCGAGCGCATATTCGAAAGA
ACTGGCATCACGCTTGTGGCATCATTCAGCGGCAACTCGACTGTCGGTCAGATTGAAAATGCTCATACAGCCGATCTTAA
CGTGATTCTCTGTCACCGGTCGATCAACTACATGGGCGAGATGATGGAAACCAAATACGGTATCCCGTGGATGAAAGTGA
ACTTTGTCGGCGCTGAATCCACAGCCAAATCACTCAGAAAAATTGCTGAATATTTTGGCGATGAAGAGCTGAAAGCCCGG
GTTGAAGCGGTAATTGCCGAAGAGATGCCAAAGGTGAAAGCGGTAATTGATGAAATCAGACCAAGAACCGAAGGCAAGAC
CGCCATGCTTTTTGTTGGCGGGTCAAGGGCTCACCACTATCAGGATCTTTTCAGCGAGCTTGGAATGACAACGGTAGCAG
CAGGGTATGAATTCGCACACCGCGACGACTATGAAGGGCGCCATGTTCTGCCCGGCATAAAAATCGATGCCGACAGCAAG
AACATCGAGGAGCTTAAAGTCACTGCGGATCCGGAACTCTACAATCCGAGAAAAAGTGAAGCCGAGCTTGAGGCGCTGAA
AGAAAAAGGACTCGAGATCAACGGTTACGAAGGAATGATGAAGCAGATGCTGAAAAAAACGCTCGTTGTTGACGACGTCA
GCCACTATGAATCGGAAAGACTGATCGAGATCTACAAGCCGGATATCTTCTGTGCAGGCATCAAGGAGAAATATGTCGTG
CAGAAAATGGGCGTTCCGCTCAAGCAGCTTCACAGCTATGACTACGGCGGTCCTTACACCGGTTTTGAAGGCGCACAGAA
CTTCTACCGGGATATCGACCGGATGGTGAACAATCCCGTCTGGAAGCTCATCAAGGCCCCGTGGCAGAAAGCGGAAAACG
GATCATCGACAGCATTGGAAGCGAGTTACGTCACTCACTAA

Upstream 100 bases:

>100_bases
CGCCGATCCTTGAAACCATCAGGGTCAGAACAGGCGAAAAAGATGAACAGGCGCTGAACTGAACGGAGAACAATTTAACG
GTGTAAACGGAGAGAGTGAC

Downstream 100 bases:

>100_bases
ACAGGAAAACGGAGACTATTGCATTATGTTATTAAGACACACGACAAAAGAGGTTAAAGAGCGTGAGGGGCTGACGATCA
ATCCGGCAAAAACCTGCCAG

Product: nitrogenase molybdenum-iron protein alpha chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MQSYKNLPDPSLVREDLIQKYPTKVAKKRNKSIVINDPETIPEVQANVRTVPGIITQRGCCYAGCKGVVLGPTRDIVNIV
HGPIGCSFYAWLTRRNQTRPETPEAENYITYCFSTDMQEENVVFGGEKKLKQAIQEAYDLFHPKAIAIFSTCPVGLIGDD
VHAASKEMRDKFGDCNVFGFSCEGYRGVSQSAGHHIANNGVFKHMVGRNNAVKEGKFKLNLLGEYNIGGDAFEIERIFER
TGITLVASFSGNSTVGQIENAHTADLNVILCHRSINYMGEMMETKYGIPWMKVNFVGAESTAKSLRKIAEYFGDEELKAR
VEAVIAEEMPKVKAVIDEIRPRTEGKTAMLFVGGSRAHHYQDLFSELGMTTVAAGYEFAHRDDYEGRHVLPGIKIDADSK
NIEELKVTADPELYNPRKSEAELEALKEKGLEINGYEGMMKQMLKKTLVVDDVSHYESERLIEIYKPDIFCAGIKEKYVV
QKMGVPLKQLHSYDYGGPYTGFEGAQNFYRDIDRMVNNPVWKLIKAPWQKAENGSSTALEASYVTH

Sequences:

>Translated_546_residues
MQSYKNLPDPSLVREDLIQKYPTKVAKKRNKSIVINDPETIPEVQANVRTVPGIITQRGCCYAGCKGVVLGPTRDIVNIV
HGPIGCSFYAWLTRRNQTRPETPEAENYITYCFSTDMQEENVVFGGEKKLKQAIQEAYDLFHPKAIAIFSTCPVGLIGDD
VHAASKEMRDKFGDCNVFGFSCEGYRGVSQSAGHHIANNGVFKHMVGRNNAVKEGKFKLNLLGEYNIGGDAFEIERIFER
TGITLVASFSGNSTVGQIENAHTADLNVILCHRSINYMGEMMETKYGIPWMKVNFVGAESTAKSLRKIAEYFGDEELKAR
VEAVIAEEMPKVKAVIDEIRPRTEGKTAMLFVGGSRAHHYQDLFSELGMTTVAAGYEFAHRDDYEGRHVLPGIKIDADSK
NIEELKVTADPELYNPRKSEAELEALKEKGLEINGYEGMMKQMLKKTLVVDDVSHYESERLIEIYKPDIFCAGIKEKYVV
QKMGVPLKQLHSYDYGGPYTGFEGAQNFYRDIDRMVNNPVWKLIKAPWQKAENGSSTALEASYVTH
>Mature_546_residues
MQSYKNLPDPSLVREDLIQKYPTKVAKKRNKSIVINDPETIPEVQANVRTVPGIITQRGCCYAGCKGVVLGPTRDIVNIV
HGPIGCSFYAWLTRRNQTRPETPEAENYITYCFSTDMQEENVVFGGEKKLKQAIQEAYDLFHPKAIAIFSTCPVGLIGDD
VHAASKEMRDKFGDCNVFGFSCEGYRGVSQSAGHHIANNGVFKHMVGRNNAVKEGKFKLNLLGEYNIGGDAFEIERIFER
TGITLVASFSGNSTVGQIENAHTADLNVILCHRSINYMGEMMETKYGIPWMKVNFVGAESTAKSLRKIAEYFGDEELKAR
VEAVIAEEMPKVKAVIDEIRPRTEGKTAMLFVGGSRAHHYQDLFSELGMTTVAAGYEFAHRDDYEGRHVLPGIKIDADSK
NIEELKVTADPELYNPRKSEAELEALKEKGLEINGYEGMMKQMLKKTLVVDDVSHYESERLIEIYKPDIFCAGIKEKYVV
QKMGVPLKQLHSYDYGGPYTGFEGAQNFYRDIDRMVNNPVWKLIKAPWQKAENGSSTALEASYVTH

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR010143
- InterPro:   IPR000318
- InterPro:   IPR005972 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 61196; Mature: 61196

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSYKNLPDPSLVREDLIQKYPTKVAKKRNKSIVINDPETIPEVQANVRTVPGIITQRGC
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCHHCCHHHHCCCC
CYAGCKGVVLGPTRDIVNIVHGPIGCSFYAWLTRRNQTRPETPEAENYITYCFSTDMQEE
HHHCCCCEEECCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCC
NVVFGGEKKLKQAIQEAYDLFHPKAIAIFSTCPVGLIGDDVHAASKEMRDKFGDCNVFGF
CEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEE
SCEGYRGVSQSAGHHIANNGVFKHMVGRNNAVKEGKFKLNLLGEYNIGGDAFEIERIFER
CCCCCCCCCHHCCCCHHCCCHHHHHHCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
TGITLVASFSGNSTVGQIENAHTADLNVILCHRSINYMGEMMETKYGIPWMKVNFVGAES
CCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEEEECCHH
TAKSLRKIAEYFGDEELKARVEAVIAEEMPKVKAVIDEIRPRTEGKTAMLFVGGSRAHHY
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHH
QDLFSELGMTTVAAGYEFAHRDDYEGRHVLPGIKIDADSKNIEELKVTADPELYNPRKSE
HHHHHHCCCHHHHCCCHHHCCCCCCCCEECCCCEECCCCCCCEEEEEECCCCCCCCCCCH
AELEALKEKGLEINGYEGMMKQMLKKTLVVDDVSHYESERLIEIYKPDIFCAGIKEKYVV
HHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCHHHHHH
QKMGVPLKQLHSYDYGGPYTGFEGAQNFYRDIDRMVNNPVWKLIKAPWQKAENGSSTALE
HHHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEE
ASYVTH
ECCCCC
>Mature Secondary Structure
MQSYKNLPDPSLVREDLIQKYPTKVAKKRNKSIVINDPETIPEVQANVRTVPGIITQRGC
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCHHCCHHHHCCCC
CYAGCKGVVLGPTRDIVNIVHGPIGCSFYAWLTRRNQTRPETPEAENYITYCFSTDMQEE
HHHCCCCEEECCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCC
NVVFGGEKKLKQAIQEAYDLFHPKAIAIFSTCPVGLIGDDVHAASKEMRDKFGDCNVFGF
CEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEE
SCEGYRGVSQSAGHHIANNGVFKHMVGRNNAVKEGKFKLNLLGEYNIGGDAFEIERIFER
CCCCCCCCCHHCCCCHHCCCHHHHHHCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
TGITLVASFSGNSTVGQIENAHTADLNVILCHRSINYMGEMMETKYGIPWMKVNFVGAES
CCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEEEECCHH
TAKSLRKIAEYFGDEELKARVEAVIAEEMPKVKAVIDEIRPRTEGKTAMLFVGGSRAHHY
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHH
QDLFSELGMTTVAAGYEFAHRDDYEGRHVLPGIKIDADSKNIEELKVTADPELYNPRKSE
HHHHHHCCCHHHHCCCHHHCCCCCCCCEECCCCEECCCCCCCEEEEEECCCCCCCCCCCH
AELEALKEKGLEINGYEGMMKQMLKKTLVVDDVSHYESERLIEIYKPDIFCAGIKEKYVV
HHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCHHHHHH
QKMGVPLKQLHSYDYGGPYTGFEGAQNFYRDIDRMVNNPVWKLIKAPWQKAENGSSTALE
HHHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEE
ASYVTH
ECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3473447; 7026551; 3457003; 2840948; 8393705 [H]