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The map label for this gene is hupE [H]

Identifier: 119356559

GI number: 119356559

Start: 826635

End: 827678

Strand: Direct

Name: hupE [H]

Synonym: Cpha266_0725

Alternate gene names: 119356559

Gene position: 826635-827678 (Clockwise)

Preceding gene: 119356558

Following gene: 119356567

Centisome position: 26.38

GC content: 53.26

Gene sequence:

>1044_bases
ATGACGATTGAATTGAGCTGTCCTGTTCCGATCAGCAATCATGAGACTGTCCAGATGGCCCATGGCGCGGGAGGAAGGCT
CTCGCAGGATCTCATGCGAAGAATCTTTATGCCGCATCTCCATAACATTTTTCTTGATCTTCTTGACGATCAGGCGAAAC
TTGAGCTCCCTCACGGGAGAGTCGCATTTACAACAGATACCTATGTTGTCAGTCCGATTTTTTTTCCCGGAGGAACGATC
GGTGAACTGGCCGTTAACGGAACGGTGAACGATCTGGCAGCAGGCGGTGCCATACCGAAGTATCTCAGCGCAGGATTTGT
GCTTGAGGAGGGGTTCTCTCTTCCTGAACTCGACAGGGTGGTAGCGAGTATGGCCGGGGCAGCTCGAAAGGCTGGTGTGC
AGATTGTGACCGGTGATACCAAGGTTGTTGAGAAAGGTCAGTGTGACAAAGTATTTATCAATACCTCGGGTATCGGCGTC
GTGCGTGAAGGTGTCGCCGTTTCATGCCGGAATCTGCAGCCTGGTGACGCTGTCATTCTTTCGGGAAATGTTGGTGATCA
TGGTATGGCGATCATGACTTCAAGAGAGGGACTCTCTTTTCAATCGAGGATTTCAAGCGACTGTGCCGCACTTAACGGCA
TGATTGCCGATGTGCTTGACGCCGTTCCTTCCATCCATGCCATGCGTGATCCTACCAGGGGAGGCGTTGCGGCCACTCTC
AATGAGCTCGCTCTTGCTTCATCGGTTGGTATTGCCATAGATGAAGCGGCTATTCCCGTTCGTCCCGATGTGCGCGGTGC
CTGCGAACTGCTCGGTATCGATCCGATGCATGTGGCAAATGAGGGTAAGCTTGTTGTTGTTGTGCAGGCCAGTGAAGCTG
AAAAGGTGCTTGAGGCCATGCACTCATCTGAACACGGTCGGGATGCTGTCAGGATCGGCTCAGTCGTCCGTGATCATCCG
GGAATGGTTATCATGCGCACCGTGTTCGGCACCCGCAGGATCGTTGATCTGCCCCTTGGGGAGCAGCTTCCACGGATTTG
CTGA

Upstream 100 bases:

>100_bases
GACGCGAGTGCAATCCACAGCACCCTCTTGGAGCGACAATGGTTTCTTCGGAAGGTGCTTGTGCCGCATACTATAACTAC
CATCGTAACCCATTTTAGTT

Downstream 100 bases:

>100_bases
TGCCTTGCGCACCTCTCTGAACTTCATTCGTCAGGTGAGTTTCAAAGAGGTGCCGATGTTCGATATCCTATATATTCAAT
GCCCGTTTTTCAAGAGCAAG

Product: hydrogenase expression/formation protein HypE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MTIELSCPVPISNHETVQMAHGAGGRLSQDLMRRIFMPHLHNIFLDLLDDQAKLELPHGRVAFTTDTYVVSPIFFPGGTI
GELAVNGTVNDLAAGGAIPKYLSAGFVLEEGFSLPELDRVVASMAGAARKAGVQIVTGDTKVVEKGQCDKVFINTSGIGV
VREGVAVSCRNLQPGDAVILSGNVGDHGMAIMTSREGLSFQSRISSDCAALNGMIADVLDAVPSIHAMRDPTRGGVAATL
NELALASSVGIAIDEAAIPVRPDVRGACELLGIDPMHVANEGKLVVVVQASEAEKVLEAMHSSEHGRDAVRIGSVVRDHP
GMVIMRTVFGTRRIVDLPLGEQLPRIC

Sequences:

>Translated_347_residues
MTIELSCPVPISNHETVQMAHGAGGRLSQDLMRRIFMPHLHNIFLDLLDDQAKLELPHGRVAFTTDTYVVSPIFFPGGTI
GELAVNGTVNDLAAGGAIPKYLSAGFVLEEGFSLPELDRVVASMAGAARKAGVQIVTGDTKVVEKGQCDKVFINTSGIGV
VREGVAVSCRNLQPGDAVILSGNVGDHGMAIMTSREGLSFQSRISSDCAALNGMIADVLDAVPSIHAMRDPTRGGVAATL
NELALASSVGIAIDEAAIPVRPDVRGACELLGIDPMHVANEGKLVVVVQASEAEKVLEAMHSSEHGRDAVRIGSVVRDHP
GMVIMRTVFGTRRIVDLPLGEQLPRIC
>Mature_346_residues
TIELSCPVPISNHETVQMAHGAGGRLSQDLMRRIFMPHLHNIFLDLLDDQAKLELPHGRVAFTTDTYVVSPIFFPGGTIG
ELAVNGTVNDLAAGGAIPKYLSAGFVLEEGFSLPELDRVVASMAGAARKAGVQIVTGDTKVVEKGQCDKVFINTSGIGVV
REGVAVSCRNLQPGDAVILSGNVGDHGMAIMTSREGLSFQSRISSDCAALNGMIADVLDAVPSIHAMRDPTRGGVAATLN
ELALASSVGIAIDEAAIPVRPDVRGACELLGIDPMHVANEGKLVVVVQASEAEKVLEAMHSSEHGRDAVRIGSVVRDHPG
MVIMRTVFGTRRIVDLPLGEQLPRIC

Specific function: May be involved in the maturation of the nifE hydrogenase [H]

COG id: COG0309

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypE family [H]

Homologues:

Organism=Escherichia coli, GI226510967, Length=337, Percent_Identity=48.6646884272997, Blast_Score=323, Evalue=1e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR011854
- InterPro:   IPR016188 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: NA

Molecular weight: Translated: 36608; Mature: 36477

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIELSCPVPISNHETVQMAHGAGGRLSQDLMRRIFMPHLHNIFLDLLDDQAKLELPHGR
CEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCC
VAFTTDTYVVSPIFFPGGTIGELAVNGTVNDLAAGGAIPKYLSAGFVLEEGFSLPELDRV
EEEEECCEEEEEEECCCCCEEEEEECCCHHHHHCCCCCHHHHHCCEEEECCCCCCHHHHH
VASMAGAARKAGVQIVTGDTKVVEKGQCDKVFINTSGIGVVREGVAVSCRNLQPGDAVIL
HHHHHHHHHHCCEEEEECCCCEECCCCCCEEEEECCCCCEEECCCEEEECCCCCCCEEEE
SGNVGDHGMAIMTSREGLSFQSRISSDCAALNGMIADVLDAVPSIHAMRDPTRGGVAATL
ECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
NELALASSVGIAIDEAAIPVRPDVRGACELLGIDPMHVANEGKLVVVVQASEAEKVLEAM
HHHHHHHHCCEEEECCCCCCCCCCCCHHHEECCCCCEECCCCCEEEEEECCCHHHHHHHH
HSSEHGRDAVRIGSVVRDHPGMVIMRTVFGTRRIVDLPLGEQLPRIC
HCCCCCCHHHHHHHHHHCCCCEEEEEEHHCCCEEEECCCCCCCCCCC
>Mature Secondary Structure 
TIELSCPVPISNHETVQMAHGAGGRLSQDLMRRIFMPHLHNIFLDLLDDQAKLELPHGR
EEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCC
VAFTTDTYVVSPIFFPGGTIGELAVNGTVNDLAAGGAIPKYLSAGFVLEEGFSLPELDRV
EEEEECCEEEEEEECCCCCEEEEEECCCHHHHHCCCCCHHHHHCCEEEECCCCCCHHHHH
VASMAGAARKAGVQIVTGDTKVVEKGQCDKVFINTSGIGVVREGVAVSCRNLQPGDAVIL
HHHHHHHHHHCCEEEEECCCCEECCCCCCEEEEECCCCCEEECCCEEEECCCCCCCEEEE
SGNVGDHGMAIMTSREGLSFQSRISSDCAALNGMIADVLDAVPSIHAMRDPTRGGVAATL
ECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
NELALASSVGIAIDEAAIPVRPDVRGACELLGIDPMHVANEGKLVVVVQASEAEKVLEAM
HHHHHHHHCCEEEECCCCCCCCCCCCHHHEECCCCCEECCCCCEEEEEECCCHHHHHHHH
HSSEHGRDAVRIGSVVRDHPGMVIMRTVFGTRRIVDLPLGEQLPRIC
HCCCCCCHHHHHHHHHHCCCCEEEEEEHHCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1526470 [H]