Click here to switch to the map view.

The map label for this gene is carB [H]

Identifier: 119356435

GI number: 119356435

Start: 670143

End: 671999

Strand: Reverse

Name: carB [H]

Synonym: Cpha266_0599

Alternate gene names: 119356435

Gene position: 671999-670143 (Counterclockwise)

Preceding gene: 119356438

Following gene: 119356433

Centisome position: 21.44

GC content: 48.57

Gene sequence:

>1857_bases
ATGACAACTCAAGCCGTTGAACTGTCGCAAGAGGTTCTTGACCTTGCGCGGAAATTACCGGAAGAAATGCTTTTAAAGGC
AAAAAAGCACGGATTCTCCGATTATCAGATTGCCCACATTTTCAAAACCACCGAAACAGTTGTCAGGAATTTGAGAAAGC
ATCTCGGCGTTATCTCGGTTTTTAAAACCGTTGACACCTGCGCTGCAGAATTCGATGCAAAAACTCCCTACCACTATTCA
ACCTATGAAGAGGAAAATGAATCCGTCTCTTCAGACCGTAAAAAAGTCATTATCCTCGGTGGCGGCCCCAACCGCATTGG
CCAGGGTATTGAATTTGACTACTGCTGTGTTCAGGCTGTGTTCGCCCTCAAAGAGGCTGGATATGAGACCATTATGGTTA
ACTGCAACCCTGAAACCGTCTCTACCGATTACGATATTGCCGATAAACTCTACTTTGAACCGCTTACGTTCGAAGACACC
ATCCGTATTATCGAGCACGAAAAACCGCTTGGTGTCATCGTCAGTTTCGGAGGACAGACCCCGCTGAAGCTCTCGACAAA
ACTTGAGGAAGCCGGCGTAACCATTCTCGGCACCTCTTCGACCGGTATCGACCTTGCAGAAGATCGTAAAAAGTTCGGGG
CACTCCTGGAAAAGCTTGCTATTCCGCATCCGGATTACGGAACAGCCATCTGCTTTGAAGAAGCTCAGATGATTACCAGA
AGAATAGGCTATCCCGTACTTGTGCGTCCCAGTTATGTGCTTGGCGGAAGGGCAATGAAAATCATTTACAGTGACGACTC
CCTCAAAGAATATGTCGATCAGGCGCTCTTCATTTCCGAGAAATATCCGCTGCTGATCGATCGCTTTCTGGAAACGGCCG
TCGAGTTCGACATTGATGCAATTGCAGACAGCAGCGACTGCGTCATCAGCGGCATCATGCAGCACGTCGAGGCAGCCGGC
ATTCACAGCGGCGACTCAACATCCATACTGCCATACCACAATATCAGTCCGGAAGTCATCGCGACCATGAAAACCTATAC
CCAGATACTTGCGGCAAATCTTCATGTTGTGGGTCTGATGAACGTTCAGTATGCCGTACAGAACGATCATGTGTATGTGC
TCGAAGTAAACCCCAGGGCAAGCCGTACCGTTCCGTTTGTTGGTAAAGCCACTGCGGTTCCTGTAGTTAAAATTGCGACC
CGTGTCATGCTGGGCGAAAAACTCAGCGACCTTCGCCAGCAATACGATCTTAAAGACTGCGATGAACTCGGCATGAAACA
TCTTGCCATCAAAGAACCGGTCTTTCCGTTCTCCAAGTTTGTCAAATCAGGAGTATATCTTGGACCGGAAATGCGCTCTA
CAGGTGAAGCCATGAGCCTCGCGGACCAGTTTCCCGAAGCCTTTGCAAAAGCCTATCAGGCCGCAAATATGGAGCTTCCT
CTCTCTGGCTCTGTTTTTATAAGTGTCAACAATCAGGATAAAAATCAGCGAATTATCACGATCGCACGGGAACTGTTCCG
GATGGACTTCGATCTTGTTGCCACAGCCGGAACCCATCAGTTTCTCATCGATAACGGTATTGAGTGCAAAAAGGTATTCA
AGGTCGGAGAAGAAGGAAGACCAAACATCTTCGATATCATCAAACACGGCAAAATAGACTTTGTCATCAATACGCCGAGA
GGCGAAAAAGCCCTCCACGATGAAGAGGCTATCGGAGCAGCGTCAGTACTCAGTAATGTACCGTTCGTTACCACCATCGA
GGCTGCCGAGGCTTCGGTGCAGGCCATAGACTGCATCCGCCGACAGGAGTTCGGCGTTAAAAGCCTTCAGGAGTATTCCT
CCTATCGCAACCGATAA

Upstream 100 bases:

>100_bases
CGGCATTTTTATTATACTCCACCGATTTCATAAAACCCTGCTGAAATGTTACCTTAAGAAACAGCCATATTCCGAAGCAT
ATACAACCTATCGACACTGC

Downstream 100 bases:

>100_bases
AAGCTTCAACAATACTTCGAGCTTCAGCATAAATGTCCGGATCCGAGCTTGCTCTCGGACCGGCCACATTGAGAGTCGCA
AGACCGTGACGGACTATCCA

Product: carbamoyl-phosphate synthase large subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase ammonia chain [H]

Number of amino acids: Translated: 618; Mature: 617

Protein sequence:

>618_residues
MTTQAVELSQEVLDLARKLPEEMLLKAKKHGFSDYQIAHIFKTTETVVRNLRKHLGVISVFKTVDTCAAEFDAKTPYHYS
TYEEENESVSSDRKKVIILGGGPNRIGQGIEFDYCCVQAVFALKEAGYETIMVNCNPETVSTDYDIADKLYFEPLTFEDT
IRIIEHEKPLGVIVSFGGQTPLKLSTKLEEAGVTILGTSSTGIDLAEDRKKFGALLEKLAIPHPDYGTAICFEEAQMITR
RIGYPVLVRPSYVLGGRAMKIIYSDDSLKEYVDQALFISEKYPLLIDRFLETAVEFDIDAIADSSDCVISGIMQHVEAAG
IHSGDSTSILPYHNISPEVIATMKTYTQILAANLHVVGLMNVQYAVQNDHVYVLEVNPRASRTVPFVGKATAVPVVKIAT
RVMLGEKLSDLRQQYDLKDCDELGMKHLAIKEPVFPFSKFVKSGVYLGPEMRSTGEAMSLADQFPEAFAKAYQAANMELP
LSGSVFISVNNQDKNQRIITIARELFRMDFDLVATAGTHQFLIDNGIECKKVFKVGEEGRPNIFDIIKHGKIDFVINTPR
GEKALHDEEAIGAASVLSNVPFVTTIEAAEASVQAIDCIRRQEFGVKSLQEYSSYRNR

Sequences:

>Translated_618_residues
MTTQAVELSQEVLDLARKLPEEMLLKAKKHGFSDYQIAHIFKTTETVVRNLRKHLGVISVFKTVDTCAAEFDAKTPYHYS
TYEEENESVSSDRKKVIILGGGPNRIGQGIEFDYCCVQAVFALKEAGYETIMVNCNPETVSTDYDIADKLYFEPLTFEDT
IRIIEHEKPLGVIVSFGGQTPLKLSTKLEEAGVTILGTSSTGIDLAEDRKKFGALLEKLAIPHPDYGTAICFEEAQMITR
RIGYPVLVRPSYVLGGRAMKIIYSDDSLKEYVDQALFISEKYPLLIDRFLETAVEFDIDAIADSSDCVISGIMQHVEAAG
IHSGDSTSILPYHNISPEVIATMKTYTQILAANLHVVGLMNVQYAVQNDHVYVLEVNPRASRTVPFVGKATAVPVVKIAT
RVMLGEKLSDLRQQYDLKDCDELGMKHLAIKEPVFPFSKFVKSGVYLGPEMRSTGEAMSLADQFPEAFAKAYQAANMELP
LSGSVFISVNNQDKNQRIITIARELFRMDFDLVATAGTHQFLIDNGIECKKVFKVGEEGRPNIFDIIKHGKIDFVINTPR
GEKALHDEEAIGAASVLSNVPFVTTIEAAEASVQAIDCIRRQEFGVKSLQEYSSYRNR
>Mature_617_residues
TTQAVELSQEVLDLARKLPEEMLLKAKKHGFSDYQIAHIFKTTETVVRNLRKHLGVISVFKTVDTCAAEFDAKTPYHYST
YEEENESVSSDRKKVIILGGGPNRIGQGIEFDYCCVQAVFALKEAGYETIMVNCNPETVSTDYDIADKLYFEPLTFEDTI
RIIEHEKPLGVIVSFGGQTPLKLSTKLEEAGVTILGTSSTGIDLAEDRKKFGALLEKLAIPHPDYGTAICFEEAQMITRR
IGYPVLVRPSYVLGGRAMKIIYSDDSLKEYVDQALFISEKYPLLIDRFLETAVEFDIDAIADSSDCVISGIMQHVEAAGI
HSGDSTSILPYHNISPEVIATMKTYTQILAANLHVVGLMNVQYAVQNDHVYVLEVNPRASRTVPFVGKATAVPVVKIATR
VMLGEKLSDLRQQYDLKDCDELGMKHLAIKEPVFPFSKFVKSGVYLGPEMRSTGEAMSLADQFPEAFAKAYQAANMELPL
SGSVFISVNNQDKNQRIITIARELFRMDFDLVATAGTHQFLIDNGIECKKVFKVGEEGRPNIFDIIKHGKIDFVINTPRG
EKALHDEEAIGAASVLSNVPFVTTIEAAEASVQAIDCIRRQEFGVKSLQEYSSYRNR

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0458

COG function: function code EF; Carbamoylphosphate synthase large subunit (split gene in MJ)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ATP-grasp domains [H]

Homologues:

Organism=Homo sapiens, GI170295797, Length=605, Percent_Identity=35.8677685950413, Blast_Score=381, Evalue=1e-105,
Organism=Homo sapiens, GI21361331, Length=605, Percent_Identity=35.8677685950413, Blast_Score=381, Evalue=1e-105,
Organism=Homo sapiens, GI169790915, Length=605, Percent_Identity=35.8677685950413, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI18105007, Length=517, Percent_Identity=37.137330754352, Blast_Score=363, Evalue=1e-100,
Organism=Escherichia coli, GI1786216, Length=581, Percent_Identity=49.2254733218589, Blast_Score=550, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI193204318, Length=619, Percent_Identity=34.248788368336, Blast_Score=362, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6322331, Length=590, Percent_Identity=35.9322033898305, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6322569, Length=484, Percent_Identity=37.603305785124, Blast_Score=336, Evalue=6e-93,
Organism=Drosophila melanogaster, GI24642586, Length=546, Percent_Identity=36.4468864468864, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI45555749, Length=506, Percent_Identity=37.9446640316205, Blast_Score=351, Evalue=8e-97,

Paralogues:

None

Copy number: 4701 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006275
- InterPro:   IPR005479
- InterPro:   IPR005483
- InterPro:   IPR005481
- InterPro:   IPR005480
- InterPro:   IPR011607
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF00289 CPSase_L_chain; PF02786 CPSase_L_D2; PF02787 CPSase_L_D3; PF02142 MGS [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 68693; Mature: 68562

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50975 ATP_GRASP ; PS00962 RIBOSOMAL_S2_1 ; PS00866 CPSASE_1 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQAVELSQEVLDLARKLPEEMLLKAKKHGFSDYQIAHIFKTTETVVRNLRKHLGVISV
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
FKTVDTCAAEFDAKTPYHYSTYEEENESVSSDRKKVIILGGGPNRIGQGIEFDYCCVQAV
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHCCCCCHHHHHHHHH
FALKEAGYETIMVNCNPETVSTDYDIADKLYFEPLTFEDTIRIIEHEKPLGVIVSFGGQT
HHHHHCCCEEEEEECCCCCCCCCCCHHHHHEECCCCHHHHHHHHCCCCCCEEEEEECCCC
PLKLSTKLEEAGVTILGTSSTGIDLAEDRKKFGALLEKLAIPHPDYGTAICFEEAQMITR
CEEEECHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHH
RIGYPVLVRPSYVLGGRAMKIIYSDDSLKEYVDQALFISEKYPLLIDRFLETAVEFDIDA
HCCCCEEECCCEEECCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHH
IADSSDCVISGIMQHVEAAGIHSGDSTSILPYHNISPEVIATMKTYTQILAANLHVVGLM
CCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCEEEEEEE
NVQYAVQNDHVYVLEVNPRASRTVPFVGKATAVPVVKIATRVMLGEKLSDLRQQYDLKDC
EEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
DELGMKHLAIKEPVFPFSKFVKSGVYLGPEMRSTGEAMSLADQFPEAFAKAYQAANMELP
HHHCCHHHHHCCCCCHHHHHHHCCCEECCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC
LSGSVFISVNNQDKNQRIITIARELFRMDFDLVATAGTHQFLIDNGIECKKVFKVGEEGR
CCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHEEECCCCEEEECCCCCHHHHHHCCCCCC
PNIFDIIKHGKIDFVINTPRGEKALHDEEAIGAASVLSNVPFVTTIEAAEASVQAIDCIR
CCHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHH
RQEFGVKSLQEYSSYRNR
HHHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TTQAVELSQEVLDLARKLPEEMLLKAKKHGFSDYQIAHIFKTTETVVRNLRKHLGVISV
CCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
FKTVDTCAAEFDAKTPYHYSTYEEENESVSSDRKKVIILGGGPNRIGQGIEFDYCCVQAV
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHCCCCCHHHHHHHHH
FALKEAGYETIMVNCNPETVSTDYDIADKLYFEPLTFEDTIRIIEHEKPLGVIVSFGGQT
HHHHHCCCEEEEEECCCCCCCCCCCHHHHHEECCCCHHHHHHHHCCCCCCEEEEEECCCC
PLKLSTKLEEAGVTILGTSSTGIDLAEDRKKFGALLEKLAIPHPDYGTAICFEEAQMITR
CEEEECHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHH
RIGYPVLVRPSYVLGGRAMKIIYSDDSLKEYVDQALFISEKYPLLIDRFLETAVEFDIDA
HCCCCEEECCCEEECCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHH
IADSSDCVISGIMQHVEAAGIHSGDSTSILPYHNISPEVIATMKTYTQILAANLHVVGLM
CCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCEEEEEEE
NVQYAVQNDHVYVLEVNPRASRTVPFVGKATAVPVVKIATRVMLGEKLSDLRQQYDLKDC
EEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
DELGMKHLAIKEPVFPFSKFVKSGVYLGPEMRSTGEAMSLADQFPEAFAKAYQAANMELP
HHHCCHHHHHCCCCCHHHHHHHCCCEECCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC
LSGSVFISVNNQDKNQRIITIARELFRMDFDLVATAGTHQFLIDNGIECKKVFKVGEEGR
CCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHEEECCCCEEEECCCCCHHHHHHCCCCCC
PNIFDIIKHGKIDFVINTPRGEKALHDEEAIGAASVLSNVPFVTTIEAAEASVQAIDCIR
CCHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHH
RQEFGVKSLQEYSSYRNR
HHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]