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The map label for this gene is pnp

Identifier: 119356388

GI number: 119356388

Start: 615226

End: 617424

Strand: Reverse

Name: pnp

Synonym: Cpha266_0552

Alternate gene names: 119356388

Gene position: 617424-615226 (Counterclockwise)

Preceding gene: 119356389

Following gene: 119356387

Centisome position: 19.7

GC content: 50.75

Gene sequence:

>2199_bases
ATGATTATCAACAAAGCAATTGATCTTGGCAAGGGCAAGATACTCTCGATCGAAACCGGTAAAATGGCAAAACAGGCCGA
TGGCGCCGCCCTTGTACGCCTTGGTGATACCATGGTGCTCGCCACGGTTGTATCAAGTAAAACTCCTCCTCCTGCCAATC
AGGACTATTTTCCGCTTCAGGTCGAGTACCGCGAAAAATACTCCGCTGCGGGTAAATTCCCTGGCGGTTTTTTTAAACGT
GAAGGACGCCCTTCCGAAAAAGAAATTCTCACGGCCCGACTTATCGACAGAGCACTCAGACCGCTCTTCCCCGACGGTTA
CTTATTTGAAACGCAGATCATCGTCACGGTATTCTCTTCAGACCAGATCAACGATGCCGACGTCCTTGGCGGCGTTGCCG
CCTCGGCGGCAATCATGGTTTCCGACATTCCTTTCCATAACTCGATGTCGGAAGTCCGTGTGGGAAGAATCAATGGAGAG
TTCATTGTCAACCCGAATATCAACGAACTGCAGGGAAGCGACATCGATATCTGTATCGGTGGCACAGCCAACACCATCTG
CATGCTTGAAGGCGAAATGAAGGAGATCTCTGAAGCCGAGATGCTTGACGCCATAAAATTCGGCCACGAAGCAATTCGTA
AAATCTGCGCGCTGCAGGACGAAATGGCTGCTGAAATAGCAAGGCCCCAACGCTCTTTTGCTCCGGTAAAAGCACCGGCA
AAACTCAAAGAGGTTATTGCCGGCCTTTCCGAAACCCGGCTGAAGGAACTTGCCTATACTCCACTCTGCAAGGAAGATCG
TGCCGAAAAAACCGCTTCCGTCTATAAAGAGACTCTCCAGTCAACGCTTGAGCTCTTCAAAGCTCTTCTGACACCTGAAG
AAATTGCAGCAGAACCTGAAAAGGCACTCTGTCTGAACGCACATATCATCGAGGAGGAGATCCACGCTGTCGAGAAAAAA
GTTATGCGACACATGATTCTTGATGACGGCAAACGGCTTGACGGAAGAACCCTTGACGAAATTCGCCCTATCAGCATTGA
ACTCGGCATCATTCCGAGAGCACACGGCTCAGCCCTGTTCACCAGAGGGGAAACCCAGGCACTCGTCACCATTACGCTTG
GCACAAAAAAAGATGCGCAGTCGGTTGACACCCTAACAGACAGTGCCGACAAGCGCTTCATGCTGCACTATAACTTTCCG
CCATTTTCCGTTGGTGAAACCGGCAGGGTCGGAGGAACGGGCCGCAGAGAGATTGGACACGGAAACCTTGCCGAACGTTC
AATCAAAATGGTCTCCCCATCAGAAACCGATTTCCCCTATACCATCCGCATCGTTTCTGATATTCTTGAATCAAACGGCT
CATCATCAATGGCTTCGGTATGCGGCGGAACCCTGGCCCTGATGGATGGCGGCGTTCCGATAAGAAAACCGGTCTCCGGA
ATCGCCATGGGACTGATCAAGGAAGGAGATGCCTACAGCGTGCTTTCCGATATTCTGGGCAACGAAGATCATCTTGGCGA
TATGGATTTCAAGGTGGCTGGTACAGAAGACGGCATCACCGCATGCCAGATGGATATCAAGATTGACGGCCTTGACTACC
ACATCCTCGAAACCGCTCTGGAACAGGCACGCAAAGGCAGACTGCATATTCTCGACAAAATGGAAGTTGCCATACCGATA
TCACGCGGCGAACTTGCACAATATGCGCCTCGACTCACCTCCATACAGATTCCGGTTGATGCCATCGGACTGATTATCGG
TAAAGGAGGAGAAACCATTCGCAGCATTACTGAAGAGACCGGCGCAGAGATCAATATTGAGGATGACGGCACCGTTACCA
TTGCCTGTTCGAGCCCGGAAGGCACCAATGCCGCAGTTGAGACCATCAAAACGCTCATCTCCAAACCCGAAATCGGCAAT
ACCTACCTTGGCAAGGTTCGCGACATTCGCGACGAACTCGGCGCCTTTGTAGAGTTTCTGCCTAAAACCGACGGACTGGT
TCACATTTCGGAAATATCGAAAGAACGGGTTACAAAGGTCAGCGATCACCTTAAAGTCGGAGACAGAGTCAAAGTCAAAC
TCGTTGACATCCGTAAAGACCCGCGTACCGGAAAAAACCGCTTTGCGCTCTCCATAAAAGCAGTGGAATCGGAACCGGAA
AAATCCGACGAGAACAAGGCTGGCACAGAAGGAAACTGA

Upstream 100 bases:

>100_bases
ACCGTCAGAACGGTCATAATGCAACACAGCCGCAAACACGGCAATGGACAGCATGGATTACACAGGAAAACACTTTTAAC
ACTAACCACAAACGATTTCC

Downstream 100 bases:

>100_bases
CCGGTTAATCACAAAAAAAGCAGTGCAGGAAATCCTGCACTGCTTTTTTTATAAAATAAAATGCACGACATCAAGAGCAT
ATGCGTTACGATTTTTCAAC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 732; Mature: 732

Protein sequence:

>732_residues
MIINKAIDLGKGKILSIETGKMAKQADGAALVRLGDTMVLATVVSSKTPPPANQDYFPLQVEYREKYSAAGKFPGGFFKR
EGRPSEKEILTARLIDRALRPLFPDGYLFETQIIVTVFSSDQINDADVLGGVAASAAIMVSDIPFHNSMSEVRVGRINGE
FIVNPNINELQGSDIDICIGGTANTICMLEGEMKEISEAEMLDAIKFGHEAIRKICALQDEMAAEIARPQRSFAPVKAPA
KLKEVIAGLSETRLKELAYTPLCKEDRAEKTASVYKETLQSTLELFKALLTPEEIAAEPEKALCLNAHIIEEEIHAVEKK
VMRHMILDDGKRLDGRTLDEIRPISIELGIIPRAHGSALFTRGETQALVTITLGTKKDAQSVDTLTDSADKRFMLHYNFP
PFSVGETGRVGGTGRREIGHGNLAERSIKMVSPSETDFPYTIRIVSDILESNGSSSMASVCGGTLALMDGGVPIRKPVSG
IAMGLIKEGDAYSVLSDILGNEDHLGDMDFKVAGTEDGITACQMDIKIDGLDYHILETALEQARKGRLHILDKMEVAIPI
SRGELAQYAPRLTSIQIPVDAIGLIIGKGGETIRSITEETGAEINIEDDGTVTIACSSPEGTNAAVETIKTLISKPEIGN
TYLGKVRDIRDELGAFVEFLPKTDGLVHISEISKERVTKVSDHLKVGDRVKVKLVDIRKDPRTGKNRFALSIKAVESEPE
KSDENKAGTEGN

Sequences:

>Translated_732_residues
MIINKAIDLGKGKILSIETGKMAKQADGAALVRLGDTMVLATVVSSKTPPPANQDYFPLQVEYREKYSAAGKFPGGFFKR
EGRPSEKEILTARLIDRALRPLFPDGYLFETQIIVTVFSSDQINDADVLGGVAASAAIMVSDIPFHNSMSEVRVGRINGE
FIVNPNINELQGSDIDICIGGTANTICMLEGEMKEISEAEMLDAIKFGHEAIRKICALQDEMAAEIARPQRSFAPVKAPA
KLKEVIAGLSETRLKELAYTPLCKEDRAEKTASVYKETLQSTLELFKALLTPEEIAAEPEKALCLNAHIIEEEIHAVEKK
VMRHMILDDGKRLDGRTLDEIRPISIELGIIPRAHGSALFTRGETQALVTITLGTKKDAQSVDTLTDSADKRFMLHYNFP
PFSVGETGRVGGTGRREIGHGNLAERSIKMVSPSETDFPYTIRIVSDILESNGSSSMASVCGGTLALMDGGVPIRKPVSG
IAMGLIKEGDAYSVLSDILGNEDHLGDMDFKVAGTEDGITACQMDIKIDGLDYHILETALEQARKGRLHILDKMEVAIPI
SRGELAQYAPRLTSIQIPVDAIGLIIGKGGETIRSITEETGAEINIEDDGTVTIACSSPEGTNAAVETIKTLISKPEIGN
TYLGKVRDIRDELGAFVEFLPKTDGLVHISEISKERVTKVSDHLKVGDRVKVKLVDIRKDPRTGKNRFALSIKAVESEPE
KSDENKAGTEGN
>Mature_732_residues
MIINKAIDLGKGKILSIETGKMAKQADGAALVRLGDTMVLATVVSSKTPPPANQDYFPLQVEYREKYSAAGKFPGGFFKR
EGRPSEKEILTARLIDRALRPLFPDGYLFETQIIVTVFSSDQINDADVLGGVAASAAIMVSDIPFHNSMSEVRVGRINGE
FIVNPNINELQGSDIDICIGGTANTICMLEGEMKEISEAEMLDAIKFGHEAIRKICALQDEMAAEIARPQRSFAPVKAPA
KLKEVIAGLSETRLKELAYTPLCKEDRAEKTASVYKETLQSTLELFKALLTPEEIAAEPEKALCLNAHIIEEEIHAVEKK
VMRHMILDDGKRLDGRTLDEIRPISIELGIIPRAHGSALFTRGETQALVTITLGTKKDAQSVDTLTDSADKRFMLHYNFP
PFSVGETGRVGGTGRREIGHGNLAERSIKMVSPSETDFPYTIRIVSDILESNGSSSMASVCGGTLALMDGGVPIRKPVSG
IAMGLIKEGDAYSVLSDILGNEDHLGDMDFKVAGTEDGITACQMDIKIDGLDYHILETALEQARKGRLHILDKMEVAIPI
SRGELAQYAPRLTSIQIPVDAIGLIIGKGGETIRSITEETGAEINIEDDGTVTIACSSPEGTNAAVETIKTLISKPEIGN
TYLGKVRDIRDELGAFVEFLPKTDGLVHISEISKERVTKVSDHLKVGDRVKVKLVDIRKDPRTGKNRFALSIKAVESEPE
KSDENKAGTEGN

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=736, Percent_Identity=37.7717391304348, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI9506689, Length=174, Percent_Identity=27.5862068965517, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI145693187, Length=699, Percent_Identity=48.4978540772532, Blast_Score=645, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=744, Percent_Identity=33.6021505376344, Blast_Score=352, Evalue=5e-97,
Organism=Drosophila melanogaster, GI281362905, Length=732, Percent_Identity=35.3825136612022, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651641, Length=732, Percent_Identity=35.3825136612022, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651643, Length=732, Percent_Identity=35.3825136612022, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI161079377, Length=672, Percent_Identity=35.4166666666667, Blast_Score=389, Evalue=1e-108,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_CHLPD (A1BDY1)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_911032.1
- ProteinModelPortal:   A1BDY1
- SMR:   A1BDY1
- STRING:   A1BDY1
- GeneID:   4569419
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_0552
- NMPDR:   fig|290317.7.peg.578
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 79496; Mature: 79496

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIINKAIDLGKGKILSIETGKMAKQADGAALVRLGDTMVLATVVSSKTPPPANQDYFPLQ
CCCCCCEECCCCCEEEEECCCCHHHCCCEEEEEECCCEEEHHHHHCCCCCCCCCCEEEEE
VEYREKYSAAGKFPGGFFKREGRPSEKEILTARLIDRALRPLFPDGYLFETQIIVTVFSS
EHHHHHHCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECC
DQINDADVLGGVAASAAIMVSDIPFHNSMSEVRVGRINGEFIVNPNINELQGSDIDICIG
CCCCCHHHHHHHHHHEEEEEECCCCCCCCCCEEEEEECCEEEECCCCCCCCCCCEEEEEC
GTANTICMLEGEMKEISEAEMLDAIKFGHEAIRKICALQDEMAAEIARPQRSFAPVKAPA
CCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCH
KLKEVIAGLSETRLKELAYTPLCKEDRAEKTASVYKETLQSTLELFKALLTPEEIAAEPE
HHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
KALCLNAHIIEEEIHAVEKKVMRHMILDDGKRLDGRTLDEIRPISIELGIIPRAHGSALF
HHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEEEEEECCCCCEEE
TRGETQALVTITLGTKKDAQSVDTLTDSADKRFMLHYNFPPFSVGETGRVGGTGRREIGH
ECCCCEEEEEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCC
GNLAERSIKMVSPSETDFPYTIRIVSDILESNGSSSMASVCGGTLALMDGGVPIRKPVSG
CCCCCCEEEEECCCCCCCCEEHHHHHHHHHCCCCCHHHHHCCCEEEEECCCCCCCCCHHH
IAMGLIKEGDAYSVLSDILGNEDHLGDMDFKVAGTEDGITACQMDIKIDGLDYHILETAL
HHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEEEEEEECCCCHHHHHHHH
EQARKGRLHILDKMEVAIPISRGELAQYAPRLTSIQIPVDAIGLIIGKGGETIRSITEET
HHHHCCCEEEEECEEEEEECCCCHHHHHCCCCEEEEECHHEEEEEECCCCHHHHHHHHHC
GAEINIEDDGTVTIACSSPEGTNAAVETIKTLISKPEIGNTYLGKVRDIRDELGAFVEFL
CCEEEECCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
PKTDGLVHISEISKERVTKVSDHLKVGDRVKVKLVDIRKDPRTGKNRFALSIKAVESEPE
CCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEEEEEECCCCC
KSDENKAGTEGN
CCCCCCCCCCCC
>Mature Secondary Structure
MIINKAIDLGKGKILSIETGKMAKQADGAALVRLGDTMVLATVVSSKTPPPANQDYFPLQ
CCCCCCEECCCCCEEEEECCCCHHHCCCEEEEEECCCEEEHHHHHCCCCCCCCCCEEEEE
VEYREKYSAAGKFPGGFFKREGRPSEKEILTARLIDRALRPLFPDGYLFETQIIVTVFSS
EHHHHHHCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECC
DQINDADVLGGVAASAAIMVSDIPFHNSMSEVRVGRINGEFIVNPNINELQGSDIDICIG
CCCCCHHHHHHHHHHEEEEEECCCCCCCCCCEEEEEECCEEEECCCCCCCCCCCEEEEEC
GTANTICMLEGEMKEISEAEMLDAIKFGHEAIRKICALQDEMAAEIARPQRSFAPVKAPA
CCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCH
KLKEVIAGLSETRLKELAYTPLCKEDRAEKTASVYKETLQSTLELFKALLTPEEIAAEPE
HHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
KALCLNAHIIEEEIHAVEKKVMRHMILDDGKRLDGRTLDEIRPISIELGIIPRAHGSALF
HHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEEEEEECCCCCEEE
TRGETQALVTITLGTKKDAQSVDTLTDSADKRFMLHYNFPPFSVGETGRVGGTGRREIGH
ECCCCEEEEEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCC
GNLAERSIKMVSPSETDFPYTIRIVSDILESNGSSSMASVCGGTLALMDGGVPIRKPVSG
CCCCCCEEEEECCCCCCCCEEHHHHHHHHHCCCCCHHHHHCCCEEEEECCCCCCCCCHHH
IAMGLIKEGDAYSVLSDILGNEDHLGDMDFKVAGTEDGITACQMDIKIDGLDYHILETAL
HHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEEEEEEECCCCHHHHHHHH
EQARKGRLHILDKMEVAIPISRGELAQYAPRLTSIQIPVDAIGLIIGKGGETIRSITEET
HHHHCCCEEEEECEEEEEECCCCHHHHHCCCCEEEEECHHEEEEEECCCCHHHHHHHHHC
GAEINIEDDGTVTIACSSPEGTNAAVETIKTLISKPEIGNTYLGKVRDIRDELGAFVEFL
CCEEEECCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
PKTDGLVHISEISKERVTKVSDHLKVGDRVKVKLVDIRKDPRTGKNRFALSIKAVESEPE
CCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEEEEEECCCCC
KSDENKAGTEGN
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA