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The map label for this gene is nusA [H]

Identifier: 119356207

GI number: 119356207

Start: 410366

End: 411922

Strand: Direct

Name: nusA [H]

Synonym: Cpha266_0368

Alternate gene names: 119356207

Gene position: 410366-411922 (Clockwise)

Preceding gene: 119356206

Following gene: 119356208

Centisome position: 13.09

GC content: 44.06

Gene sequence:

>1557_bases
ATGGTCAGAAAGCAGGTAAAAACGGAGGGGCAGGACAGGAAAGCGCAGATTGCCAGTGCTTTCGGAGAAATCGAGCAGTC
CAAAATCTTTCTGGACAAACGTACGGAAAGTGCGGCTGTCAAAATGGATATTGCAGACCTTCTTAAAGATATCATCCAGA
AGCAGCTCAGAAAGGATTATGATCCTGAAGTTGAGGCTAATATTTTCATTAATCCCGAGCGCGGCGATTTTGAGGTTTAT
ATTCTGAAAAAGGTTGTCAGTGAAGTTGATCTCGAAAGCATTGAAATCAGCATCGATGAAGTCAGAAAAATAGACGATTC
TCTTGAACTCGGAGATTATTATGAAGAGGGCCCGATCAAGCTTGAGGATTATCTGAGCAGAAAGTCTATCCAGATTATCA
AGCAGTCTGTACAGAAAAAGGTACGGGATCTTGAGCGTCTTGTGGTGTATGAGGAGTGCCTTGAAAAAGTTGGCGAGGTT
GTGGCCGGTGAAGTATATCAGGTTCGGTCAAACGAAGTGATATTTACCTACAACACATCAAAAGATCATCGTGTTGAGCT
TGTTCTGCCAAGAGCCGAAATGATAAAAAAGGATAATCCTCGCAGAAACCCGAGGATGAAGCTCTACGTCAAGCGCATCG
AACGCGAGAAGGTTAAGGTAAGGCTTGATGACGGCGGCGTTGTCGAGCGCGATAAACCTGATGGGGGAATGAAAGTAATT
GTTTCAAGGATTGACGATCGCTTTTTGTACAAACTGTTTGAACATGAGGTTCCGGAAATTCTCGATGGTCTTATTGTGAT
AAAAGGAATTGCCCGTGTTCCAGGCGAAAGGGCAAAGGTTGCCGTTGAGTCCACCAGTGCCAGAATAGATCCTGTAGGAG
CAAGCGTAGGGTATCGCGGAAAAAGGATACAGAGTATCGTCAAGGAACTGAACAATGAGAATATCGATGTTATTTATTAT
ACCGATGAACCGCAGATTTATATAGCTCGTGCTTTGCAGCCAGCCAAGATTGATCCCTTGACGGTTCATGCCGATATTAA
AACCCGAAAGGCAAGGGTAATGCTGAAGCCGGACCAGATCAAGTATGCCATTGGAAAAAACGGTAATAACATACATCTGG
CCGAAAAGCTTACCGGATATGAAATCGATGTGTATCGCGATGTGATGGACAAGTCGATGGAAGATCCTACTGATATTGAT
ATTATTGAGTTCAGGGAAGAGTTTGGCGATGATATGATCTATCAATTGCTTGACGGTGGCCTTGATACGGCCAAGAAGGT
ACTGAAAGCAGGTGTTGAAAGGATTGAGGAGGTTTTGCTCGGCCCTTCAGCTCCAGAGGAGATTACTTTTTTTTCAAAAG
GTCGAACCAGGAGCCCGATTAAGCCAAGAGAGCGAAAGGTAACCGAAGAGGAAAAGCGGTACTGGAAAAAAATTGCCGAA
AATATTTTTAAAACCGTTAAAGAGCAGTTTACTGATGCTGATTTGCATGATCTGTTCGACGATGGAGATGAAGACAGTGA
CGATCAACAAGCCGCTGAAAGTCCGGCAGATGAATGA

Upstream 100 bases:

>100_bases
CGGCTTGACCGTGTCATCAGGGCAGTTCCGGAAGCTGAGTTGTAACCGGAGGGAGATGGGTCGGTAATATTTCTTTTTTT
AATGCACAATAAGCAAACAG

Downstream 100 bases:

>100_bases
TACAGAGCAACTGCGTACACTATAATTTGTTGCAATAAAGCGTTTTTAAATCCAGAGGAGGATGTAATGGCCCTTGAGGA
CATGGAAAAGAGATACCGGA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MVRKQVKTEGQDRKAQIASAFGEIEQSKIFLDKRTESAAVKMDIADLLKDIIQKQLRKDYDPEVEANIFINPERGDFEVY
ILKKVVSEVDLESIEISIDEVRKIDDSLELGDYYEEGPIKLEDYLSRKSIQIIKQSVQKKVRDLERLVVYEECLEKVGEV
VAGEVYQVRSNEVIFTYNTSKDHRVELVLPRAEMIKKDNPRRNPRMKLYVKRIEREKVKVRLDDGGVVERDKPDGGMKVI
VSRIDDRFLYKLFEHEVPEILDGLIVIKGIARVPGERAKVAVESTSARIDPVGASVGYRGKRIQSIVKELNNENIDVIYY
TDEPQIYIARALQPAKIDPLTVHADIKTRKARVMLKPDQIKYAIGKNGNNIHLAEKLTGYEIDVYRDVMDKSMEDPTDID
IIEFREEFGDDMIYQLLDGGLDTAKKVLKAGVERIEEVLLGPSAPEEITFFSKGRTRSPIKPRERKVTEEEKRYWKKIAE
NIFKTVKEQFTDADLHDLFDDGDEDSDDQQAAESPADE

Sequences:

>Translated_518_residues
MVRKQVKTEGQDRKAQIASAFGEIEQSKIFLDKRTESAAVKMDIADLLKDIIQKQLRKDYDPEVEANIFINPERGDFEVY
ILKKVVSEVDLESIEISIDEVRKIDDSLELGDYYEEGPIKLEDYLSRKSIQIIKQSVQKKVRDLERLVVYEECLEKVGEV
VAGEVYQVRSNEVIFTYNTSKDHRVELVLPRAEMIKKDNPRRNPRMKLYVKRIEREKVKVRLDDGGVVERDKPDGGMKVI
VSRIDDRFLYKLFEHEVPEILDGLIVIKGIARVPGERAKVAVESTSARIDPVGASVGYRGKRIQSIVKELNNENIDVIYY
TDEPQIYIARALQPAKIDPLTVHADIKTRKARVMLKPDQIKYAIGKNGNNIHLAEKLTGYEIDVYRDVMDKSMEDPTDID
IIEFREEFGDDMIYQLLDGGLDTAKKVLKAGVERIEEVLLGPSAPEEITFFSKGRTRSPIKPRERKVTEEEKRYWKKIAE
NIFKTVKEQFTDADLHDLFDDGDEDSDDQQAAESPADE
>Mature_518_residues
MVRKQVKTEGQDRKAQIASAFGEIEQSKIFLDKRTESAAVKMDIADLLKDIIQKQLRKDYDPEVEANIFINPERGDFEVY
ILKKVVSEVDLESIEISIDEVRKIDDSLELGDYYEEGPIKLEDYLSRKSIQIIKQSVQKKVRDLERLVVYEECLEKVGEV
VAGEVYQVRSNEVIFTYNTSKDHRVELVLPRAEMIKKDNPRRNPRMKLYVKRIEREKVKVRLDDGGVVERDKPDGGMKVI
VSRIDDRFLYKLFEHEVPEILDGLIVIKGIARVPGERAKVAVESTSARIDPVGASVGYRGKRIQSIVKELNNENIDVIYY
TDEPQIYIARALQPAKIDPLTVHADIKTRKARVMLKPDQIKYAIGKNGNNIHLAEKLTGYEIDVYRDVMDKSMEDPTDID
IIEFREEFGDDMIYQLLDGGLDTAKKVLKAGVERIEEVLLGPSAPEEITFFSKGRTRSPIKPRERKVTEEEKRYWKKIAE
NIFKTVKEQFTDADLHDLFDDGDEDSDDQQAAESPADE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=353, Percent_Identity=32.2946175637394, Blast_Score=176, Evalue=3e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 59543; Mature: 59543

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRKQVKTEGQDRKAQIASAFGEIEQSKIFLDKRTESAAVKMDIADLLKDIIQKQLRKDY
CCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCHHEEECHHHHHHHHHHHHHHCCC
DPEVEANIFINPERGDFEVYILKKVVSEVDLESIEISIDEVRKIDDSLELGDYYEEGPIK
CCCCCEEEEECCCCCCEEEHHHHHHHHHCCHHHEEEEHHHHHHHHCCCCCCCCCCCCCEE
LEDYLSRKSIQIIKQSVQKKVRDLERLVVYEECLEKVGEVVAGEVYQVRSNEVIFTYNTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEECC
KDHRVELVLPRAEMIKKDNPRRNPRMKLYVKRIEREKVKVRLDDGGVVERDKPDGGMKVI
CCCEEEEEECHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCEEECCCCCCCHHHH
VSRIDDRFLYKLFEHEVPEILDGLIVIKGIARVPGERAKVAVESTSARIDPVGASVGYRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCCCCCCCCCCCCCCH
KRIQSIVKELNNENIDVIYYTDEPQIYIARALQPAKIDPLTVHADIKTRKARVMLKPDQI
HHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCCCCCEEEEECCCCCCEEEEECCCCE
KYAIGKNGNNIHLAEKLTGYEIDVYRDVMDKSMEDPTDIDIIEFREEFGDDMIYQLLDGG
EEEECCCCCEEEEEHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCHHHHHHHHHCC
LDTAKKVLKAGVERIEEVLLGPSAPEEITFFSKGRTRSPIKPRERKVTEEEKRYWKKIAE
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHH
NIFKTVKEQFTDADLHDLFDDGDEDSDDQQAAESPADE
HHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHCCCCCC
>Mature Secondary Structure
MVRKQVKTEGQDRKAQIASAFGEIEQSKIFLDKRTESAAVKMDIADLLKDIIQKQLRKDY
CCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCHHEEECHHHHHHHHHHHHHHCCC
DPEVEANIFINPERGDFEVYILKKVVSEVDLESIEISIDEVRKIDDSLELGDYYEEGPIK
CCCCCEEEEECCCCCCEEEHHHHHHHHHCCHHHEEEEHHHHHHHHCCCCCCCCCCCCCEE
LEDYLSRKSIQIIKQSVQKKVRDLERLVVYEECLEKVGEVVAGEVYQVRSNEVIFTYNTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEECC
KDHRVELVLPRAEMIKKDNPRRNPRMKLYVKRIEREKVKVRLDDGGVVERDKPDGGMKVI
CCCEEEEEECHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCEEECCCCCCCHHHH
VSRIDDRFLYKLFEHEVPEILDGLIVIKGIARVPGERAKVAVESTSARIDPVGASVGYRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCCCCCCCCCCCCCCH
KRIQSIVKELNNENIDVIYYTDEPQIYIARALQPAKIDPLTVHADIKTRKARVMLKPDQI
HHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCCCCCEEEEECCCCCCEEEEECCCCE
KYAIGKNGNNIHLAEKLTGYEIDVYRDVMDKSMEDPTDIDIIEFREEFGDDMIYQLLDGG
EEEECCCCCEEEEEHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCHHHHHHHHHCC
LDTAKKVLKAGVERIEEVLLGPSAPEEITFFSKGRTRSPIKPRERKVTEEEKRYWKKIAE
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHH
NIFKTVKEQFTDADLHDLFDDGDEDSDDQQAAESPADE
HHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9242984 [H]