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The map label for this gene is ykfA [H]

Identifier: 119355897

GI number: 119355897

Start: 49083

End: 50054

Strand: Reverse

Name: ykfA [H]

Synonym: Cpha266_0047

Alternate gene names: 119355897

Gene position: 50054-49083 (Counterclockwise)

Preceding gene: 119355898

Following gene: 119355896

Centisome position: 1.6

GC content: 52.98

Gene sequence:

>972_bases
ATGCCTCGGCATTTTTTTGCCTGTATGAAAACCCTTCTGCCAAAAGCACTCCGCAAGGGAGACACCATAGGCCTGATCTC
CCCATCATCGCACTCGGCTTTTCCTGAAAGAATCGCACGGGCAATCCTGTATCTCGAAGAACGATCCTTCAAGGTAAAGC
CCTCAACGTACCTCAACTGCATTGACGTCAACCCGGCCATTGCCGACCAGCAGAAACTCAGCGACCTTCACGCCATGTTC
GAAGATACCGAAGTCGATGCGGTTATCTGTCTGAGGGGAGGGGCTGGCGCCACCCGGCTGCTGAAGAATATCGATTATGC
CCTCATCGAAGCAAATCCGAAAATCCTTATCGGTTATTCTGATATCACAGCGCTCTCCCTTGCCATTTTAGCGTATACAG
GGCTGGTCAGTTTCTCCGGCCCGATGATCGCCACAGAACTGTATGAACCGACGCCATATACCGAAGAGCACTTCTGGGGC
ATGCTCACAGACCCTCGATATGCGCTTTCGCTCAAAAACCATAGCCAGCACAGGGTGACCTGTCTCAAGCCGGGAGATGC
TTCAGGAAACCTTATCGGCGGAAACCTCTCCGTTCTCTGCTCGCTGATCGGCACCCCCTATCTCCCCATCCTTGACGATG
CGATCCTTTTCCTCGAAGATGTCAATGAACCGGCTTATCGGATAGACCGTATGCTCTCGCATCTCCTTAATGCGGGCCTG
CTCTCCCGATGCAGGGCGATTCTGTTCGGTCAGTTTACCGGCACCCCGGAAAAAGCAGGAGAAGATACCCGGCTCGAACA
TATTTTTGACTACTACGCCACGCAAGCTCACATAGAAGGTCCGGTCATGACAGGACTCTCTTACGGTCACATAGCCGATC
TCATGACGCTCCCCCTTGGAGCTCCGTTCCAGGTAACCGTACGACCCCGATATTTTTCCCTTGAGGCGCGTTATCCGGTG
ATTCATGCATAA

Upstream 100 bases:

>100_bases
CGACGGTTTTTTTGAGGTAAGCAACAATAAGGCCATACTGCTTACCGAAGAGATCTCCTGATTTCATGCTTAATGAAATA
AAAAAAAAGCCGGGGCAAGC

Downstream 100 bases:

>100_bases
CGCTCTTGCCTCTCTGAAAGCTACGTTGTATTTTATCATTTTAAACGTATTCCTCAATCTTCATGATCTTCTCCGTTTCA
ACCAGTTTATCGGCAACCCG

Product: muramoyltetrapeptide carboxypeptidase

Products: NA

Alternate protein names: LD-carboxypeptidase [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MPRHFFACMKTLLPKALRKGDTIGLISPSSHSAFPERIARAILYLEERSFKVKPSTYLNCIDVNPAIADQQKLSDLHAMF
EDTEVDAVICLRGGAGATRLLKNIDYALIEANPKILIGYSDITALSLAILAYTGLVSFSGPMIATELYEPTPYTEEHFWG
MLTDPRYALSLKNHSQHRVTCLKPGDASGNLIGGNLSVLCSLIGTPYLPILDDAILFLEDVNEPAYRIDRMLSHLLNAGL
LSRCRAILFGQFTGTPEKAGEDTRLEHIFDYYATQAHIEGPVMTGLSYGHIADLMTLPLGAPFQVTVRPRYFSLEARYPV
IHA

Sequences:

>Translated_323_residues
MPRHFFACMKTLLPKALRKGDTIGLISPSSHSAFPERIARAILYLEERSFKVKPSTYLNCIDVNPAIADQQKLSDLHAMF
EDTEVDAVICLRGGAGATRLLKNIDYALIEANPKILIGYSDITALSLAILAYTGLVSFSGPMIATELYEPTPYTEEHFWG
MLTDPRYALSLKNHSQHRVTCLKPGDASGNLIGGNLSVLCSLIGTPYLPILDDAILFLEDVNEPAYRIDRMLSHLLNAGL
LSRCRAILFGQFTGTPEKAGEDTRLEHIFDYYATQAHIEGPVMTGLSYGHIADLMTLPLGAPFQVTVRPRYFSLEARYPV
IHA
>Mature_322_residues
PRHFFACMKTLLPKALRKGDTIGLISPSSHSAFPERIARAILYLEERSFKVKPSTYLNCIDVNPAIADQQKLSDLHAMFE
DTEVDAVICLRGGAGATRLLKNIDYALIEANPKILIGYSDITALSLAILAYTGLVSFSGPMIATELYEPTPYTEEHFWGM
LTDPRYALSLKNHSQHRVTCLKPGDASGNLIGGNLSVLCSLIGTPYLPILDDAILFLEDVNEPAYRIDRMLSHLLNAGLL
SRCRAILFGQFTGTPEKAGEDTRLEHIFDYYATQAHIEGPVMTGLSYGHIADLMTLPLGAPFQVTVRPRYFSLEARYPVI
HA

Specific function: May be involved in the degradation of peptidoglycan by catalyzing the cleavage of the terminal D-alanine residue from cytoplasmic murein peptides [H]

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=285, Percent_Identity=34.7368421052632, Blast_Score=161, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 35717; Mature: 35586

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRHFFACMKTLLPKALRKGDTIGLISPSSHSAFPERIARAILYLEERSFKVKPSTYLNC
CCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEECCCCEEEE
IDVNPAIADQQKLSDLHAMFEDTEVDAVICLRGGAGATRLLKNIDYALIEANPKILIGYS
EECCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCEEEEECCCCEEEEEC
DITALSLAILAYTGLVSFSGPMIATELYEPTPYTEEHFWGMLTDPRYALSLKNHSQHRVT
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHCCEEECCCEEEEEECCCCCCEEE
CLKPGDASGNLIGGNLSVLCSLIGTPYLPILDDAILFLEDVNEPAYRIDRMLSHLLNAGL
EECCCCCCCCEECCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LSRCRAILFGQFTGTPEKAGEDTRLEHIFDYYATQAHIEGPVMTGLSYGHIADLMTLPLG
HHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHCCCC
APFQVTVRPRYFSLEARYPVIHA
CCEEEEECCEEEEEEECCCEECC
>Mature Secondary Structure 
PRHFFACMKTLLPKALRKGDTIGLISPSSHSAFPERIARAILYLEERSFKVKPSTYLNC
CHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEECCCCEEEE
IDVNPAIADQQKLSDLHAMFEDTEVDAVICLRGGAGATRLLKNIDYALIEANPKILIGYS
EECCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCEEEEECCCCEEEEEC
DITALSLAILAYTGLVSFSGPMIATELYEPTPYTEEHFWGMLTDPRYALSLKNHSQHRVT
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHCCEEECCCEEEEEECCCCCCEEE
CLKPGDASGNLIGGNLSVLCSLIGTPYLPILDDAILFLEDVNEPAYRIDRMLSHLLNAGL
EECCCCCCCCEECCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LSRCRAILFGQFTGTPEKAGEDTRLEHIFDYYATQAHIEGPVMTGLSYGHIADLMTLPLG
HHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHCCCC
APFQVTVRPRYFSLEARYPVIHA
CCEEEEECCEEEEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]