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The map label for this gene is hldE

Identifier: 118581990

GI number: 118581990

Start: 3970835

End: 3972301

Strand: Reverse

Name: hldE

Synonym: Ppro_3591

Alternate gene names: 118581990

Gene position: 3972301-3970835 (Counterclockwise)

Preceding gene: 118581991

Following gene: 118581989

Centisome position: 99.11

GC content: 64.55

Gene sequence:

>1467_bases
ATGGATCGCAAATCGATTGAATCGATCTTCAACAGGATACGGACTGCAAACTGTCTGGTGATCGGCGACCTGATGCTGGA
CGAATATCTGTGGGGCAAGGCCGAACGGATATCACCCGAAGCACCGGTGCAGGTGGTGGATGTGGTCCGCGAGGAGATGC
GTCTGGGGGGGGCCGGCAATGTGGTCAACAACCTGGTGGAACTGGGTGCCGAGGTGACGGTCTGCTCGGTGGTGGGCGAC
GACGACAACGGCCAGGCACTGCTGGAGGCCTTCGGCCGCAGGGGAGTGGCCACCGATGCCATCTTTCTCGACGCAACCCG
CCGCACCAGCCGCAAGACCCGCGTGGTGGCCGCCCACCAGCAGATTGTCAGGATCGACCGCGAGTCGCGCGTTCCGCTCT
CCGCGGAAGTCGAGCGTAGGGTCAGCGACTGGATCACGGCCAACGCAGGAGGGTTCGATGTGATCCTGCTCTCCGACTAC
CAGAAGGGGGTGCTGACCCCCGGGGTGATCTCCGCCACCCTGGCGGCGGCGCGGCCAGGAGCTATTCCGGTGTTGGTCGA
CCCCAAGGGTACCGATTTCTCCCGCTACAGCGGCGCTACCCTGCTGACCCCCAACCGCAGGGAGGCCGAGGCAGCTTCGG
GCATAGCCATCCATGATATCGAATCCCTGGTCAGGGCCGCCGGTGTGATCATGGAGCGGGTCGGCCTGGAGCATTTGCTG
ATCACCCGCAGTGAAGAGGGGATGTCCCTCTTCTCCCGTTCGGCCGCGCCGATGCATATCCCCACCGTGGCACGGGAGGT
CTTCGATGTCTCCGGCGCCGGCGATACCGTGCTGGCCTCTCTGGCCGCCGGCATGGCTGCCGGCATGGAGATGATCGAGG
CGGCCCGGCTGGCCAACATCACCGCCGGAATCGCCGTTGCCAAGCTGGGGACATCGACGGTGGCGCCGGCGGAGATCATC
AATGCCGTGGCCCTGGCCCACAGCGACAGCGATTCAAAGATCAAGAACCGCGACGTGCTGGCTGTGCTGATCGAGGCCGA
GAAGGCCCGCGGCAAGCGGATCGTCTTCACCAATGGCTGCTTCGACCTGCTGCATGCCGGTCATGTCAAGTATCTGCAGA
AGGCCCGCACCCTGGGGGATCTGCTGGTGCTGGGGCTGAACAGCGACGCATCGGTGCGGCGGCTGAAAGGTGAGAAACGC
CCGCTGATAGGCGAGCAGGAACGGGCCCACATCCTGGCGGCGCTGGACTGCATCGACTACGTGGTGATCTTCGAGGAGGA
CACGCCGCTGGAGCTGATCGCCGCCCTGAAACCGCATATCCTGGCCAAGGGGGGGGATTACACCCCCGAGGGCGTGGTGG
GCAGGGAACTGGTGGAGAGCTACGGCGGCCGGGTGGAGCTGGTGTCGTTCGTGGACGGGAAATCCACCACCAACATCATC
GAACGGGTCCTGGAGCGCTATTCCTGA

Upstream 100 bases:

>100_bases
TCTGCGACCTGGTGGAGAAGGCGTTGTTTGCCTGAGGCGGTACAGGGTTCAGGGCCAAGAGGTTTTCGGCTGAAGAGCCC
GAAGCCGGAACGGAGTGAGT

Downstream 100 bases:

>100_bases
AGGATTTGCCCCTTGTCTTATCCGTGGTTTATGGGATAAAACACGTAGCAGCTTTTCATGATATTCAGTGAACAGGAGCA
GTTATGGATCCGGAAACCGC

Product: rfaE bifunctional protein

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MDRKSIESIFNRIRTANCLVIGDLMLDEYLWGKAERISPEAPVQVVDVVREEMRLGGAGNVVNNLVELGAEVTVCSVVGD
DDNGQALLEAFGRRGVATDAIFLDATRRTSRKTRVVAAHQQIVRIDRESRVPLSAEVERRVSDWITANAGGFDVILLSDY
QKGVLTPGVISATLAAARPGAIPVLVDPKGTDFSRYSGATLLTPNRREAEAASGIAIHDIESLVRAAGVIMERVGLEHLL
ITRSEEGMSLFSRSAAPMHIPTVAREVFDVSGAGDTVLASLAAGMAAGMEMIEAARLANITAGIAVAKLGTSTVAPAEII
NAVALAHSDSDSKIKNRDVLAVLIEAEKARGKRIVFTNGCFDLLHAGHVKYLQKARTLGDLLVLGLNSDASVRRLKGEKR
PLIGEQERAHILAALDCIDYVVIFEEDTPLELIAALKPHILAKGGDYTPEGVVGRELVESYGGRVELVSFVDGKSTTNII
ERVLERYS

Sequences:

>Translated_488_residues
MDRKSIESIFNRIRTANCLVIGDLMLDEYLWGKAERISPEAPVQVVDVVREEMRLGGAGNVVNNLVELGAEVTVCSVVGD
DDNGQALLEAFGRRGVATDAIFLDATRRTSRKTRVVAAHQQIVRIDRESRVPLSAEVERRVSDWITANAGGFDVILLSDY
QKGVLTPGVISATLAAARPGAIPVLVDPKGTDFSRYSGATLLTPNRREAEAASGIAIHDIESLVRAAGVIMERVGLEHLL
ITRSEEGMSLFSRSAAPMHIPTVAREVFDVSGAGDTVLASLAAGMAAGMEMIEAARLANITAGIAVAKLGTSTVAPAEII
NAVALAHSDSDSKIKNRDVLAVLIEAEKARGKRIVFTNGCFDLLHAGHVKYLQKARTLGDLLVLGLNSDASVRRLKGEKR
PLIGEQERAHILAALDCIDYVVIFEEDTPLELIAALKPHILAKGGDYTPEGVVGRELVESYGGRVELVSFVDGKSTTNII
ERVLERYS
>Mature_488_residues
MDRKSIESIFNRIRTANCLVIGDLMLDEYLWGKAERISPEAPVQVVDVVREEMRLGGAGNVVNNLVELGAEVTVCSVVGD
DDNGQALLEAFGRRGVATDAIFLDATRRTSRKTRVVAAHQQIVRIDRESRVPLSAEVERRVSDWITANAGGFDVILLSDY
QKGVLTPGVISATLAAARPGAIPVLVDPKGTDFSRYSGATLLTPNRREAEAASGIAIHDIESLVRAAGVIMERVGLEHLL
ITRSEEGMSLFSRSAAPMHIPTVAREVFDVSGAGDTVLASLAAGMAAGMEMIEAARLANITAGIAVAKLGTSTVAPAEII
NAVALAHSDSDSKIKNRDVLAVLIEAEKARGKRIVFTNGCFDLLHAGHVKYLQKARTLGDLLVLGLNSDASVRRLKGEKR
PLIGEQERAHILAALDCIDYVVIFEEDTPLELIAALKPHILAKGGDYTPEGVVGRELVESYGGRVELVSFVDGKSTTNII
ERVLERYS

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family

Homologues:

Organism=Escherichia coli, GI1789432, Length=476, Percent_Identity=44.9579831932773, Blast_Score=379, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45550974, Length=152, Percent_Identity=30.9210526315789, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45550973, Length=152, Percent_Identity=30.9210526315789, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45550975, Length=152, Percent_Identity=30.9210526315789, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19921220, Length=75, Percent_Identity=42.6666666666667, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HLDE_PELPD (A1AV12)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_903240.1
- ProteinModelPortal:   A1AV12
- STRING:   A1AV12
- GeneID:   4574671
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_3591
- eggNOG:   COG2870
- HOGENOM:   HBG545535
- OMA:   LTPNRKE
- PhylomeDB:   A1AV12
- ProtClustDB:   CLSK828725
- HAMAP:   MF_01603
- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00125
- TIGRFAMs:   TIGR02198
- TIGRFAMs:   TIGR02199

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB

EC number: 2.7.-.-

Molecular weight: Translated: 52400; Mature: 52400

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2

Important sites: ACT_SITE 275-275

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRKSIESIFNRIRTANCLVIGDLMLDEYLWGKAERISPEAPVQVVDVVREEMRLGGAGN
CCHHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHCCCCCH
VVNNLVELGAEVTVCSVVGDDDNGQALLEAFGRRGVATDAIFLDATRRTSRKTRVVAAHQ
HHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCEEEEECHHCCCHHHHHHHHHH
QIVRIDRESRVPLSAEVERRVSDWITANAGGFDVILLSDYQKGVLTPGVISATLAAARPG
HHHHHCCCCCCCCHHHHHHHHHHHEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCC
AIPVLVDPKGTDFSRYSGATLLTPNRREAEAASGIAIHDIESLVRAAGVIMERVGLEHLL
CEEEEECCCCCCCCCCCCCEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHEE
ITRSEEGMSLFSRSAAPMHIPTVAREVFDVSGAGDTVLASLAAGMAAGMEMIEAARLANI
EECCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TAGIAVAKLGTSTVAPAEIINAVALAHSDSDSKIKNRDVLAVLIEAEKARGKRIVFTNGC
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHCCCCCEEEEECCH
FDLLHAGHVKYLQKARTLGDLLVLGLNSDASVRRLKGEKRPLIGEQERAHILAALDCIDY
HHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCE
VVIFEEDTPLELIAALKPHILAKGGDYTPEGVVGRELVESYGGRVELVSFVDGKSTTNII
EEEECCCCCHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHH
ERVLERYS
HHHHHHCC
>Mature Secondary Structure
MDRKSIESIFNRIRTANCLVIGDLMLDEYLWGKAERISPEAPVQVVDVVREEMRLGGAGN
CCHHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHCCCCCH
VVNNLVELGAEVTVCSVVGDDDNGQALLEAFGRRGVATDAIFLDATRRTSRKTRVVAAHQ
HHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCEEEEECHHCCCHHHHHHHHHH
QIVRIDRESRVPLSAEVERRVSDWITANAGGFDVILLSDYQKGVLTPGVISATLAAARPG
HHHHHCCCCCCCCHHHHHHHHHHHEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCC
AIPVLVDPKGTDFSRYSGATLLTPNRREAEAASGIAIHDIESLVRAAGVIMERVGLEHLL
CEEEEECCCCCCCCCCCCCEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHEE
ITRSEEGMSLFSRSAAPMHIPTVAREVFDVSGAGDTVLASLAAGMAAGMEMIEAARLANI
EECCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TAGIAVAKLGTSTVAPAEIINAVALAHSDSDSKIKNRDVLAVLIEAEKARGKRIVFTNGC
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHCCCCCEEEEECCH
FDLLHAGHVKYLQKARTLGDLLVLGLNSDASVRRLKGEKRPLIGEQERAHILAALDCIDY
HHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCE
VVIFEEDTPLELIAALKPHILAKGGDYTPEGVVGRELVESYGGRVELVSFVDGKSTTNII
EEEECCCCCHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHH
ERVLERYS
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA