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The map label for this gene is ttcA

Identifier: 118581899

GI number: 118581899

Start: 3867924

End: 3868733

Strand: Reverse

Name: ttcA

Synonym: Ppro_3499

Alternate gene names: 118581899

Gene position: 3868733-3867924 (Counterclockwise)

Preceding gene: 118581900

Following gene: 118581891

Centisome position: 96.53

GC content: 62.84

Gene sequence:

>810_bases
ATGCTGCGTACGCCAAAGGCGGCCAGTGACCCTATGACCTTGACGCAGCAGGAGATGCGTGAGTTGCCGCTGTTCAGGCG
GCTGCGCCATGCCGTGGGAAAAGCGGTGGCTGATTTCGCCATGATCCGTGAGGGGGACCGTATCGCGGTGGGGGTTTCGG
GGGGTAAGGACTCCTACACCCTCTTGCTGCTGCTGGAGGAACTGCGACGGCGGGCGCCCATCGACTTCCAGCTGGTGGCC
GTGATCATCGACTCCGGCTATCCCGGTTACCGGGGCGATATTGTCCGTGACTATGTGACCTCCCTGGGCATCCCCTGCCA
TCTGGAAACAACCACCCACTACGAGATCATCACCGAGAAGCGCCGCCCCGGCTCCTCCTACTGCTCCATCTGCGCCCGCC
TGAAGCGGGGGGCACTGTACGGACTGGCCGACAGCCTGGGGTGTAACAAGCTGGCCCTGGGGCACCATGGCGACGACTTC
ATCGAGACGCTGCTGCTGAACCAGTTCTTCGTCGGTTCGCTCAAGGCCATGTCGGCCAACATGCTGGCCGACAACGGCCG
CACCACCGTCATCAGGCCGCTGGTGTACGCGTCCGAGGAGGAGATCGTCGATTTCATGGGCCAGGTCGGGCTGCCGGTGG
TCAGCTGCAACTGCCCGGTGAGCGATTCCACCGACCTCAAGCGCAGGCGTATGAAGGAACTGCTCAAAGAACTGGAGCAG
GAGATCCCCCATATCAGGAGCAGCATGCTCAAGGCGCTCTCCAACGTCCATCCCCGGCATCTGCTGGACCAGCAGCTCCA
GGGTCTCTGA

Upstream 100 bases:

>100_bases
GGCAAGGAGGTGGAGTACCACCTGATCAGTTCCTCCTACGGCCACGACGCCTTCCTGCTGGAACACGAGGCGTTCTCCCC
CCTGGTGAAGCGGTTCCTGG

Downstream 100 bases:

>100_bases
TCTCCTTAAGGTAAACAGGGAGCGGTTACACAAAAAGCGCCTGAAACAGGCGCTTTTTGTGTAACCGCATTCCCGAACTG
CCGTCTATTCGTAGTAATTG

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MLRTPKAASDPMTLTQQEMRELPLFRRLRHAVGKAVADFAMIREGDRIAVGVSGGKDSYTLLLLLEELRRRAPIDFQLVA
VIIDSGYPGYRGDIVRDYVTSLGIPCHLETTTHYEIITEKRRPGSSYCSICARLKRGALYGLADSLGCNKLALGHHGDDF
IETLLLNQFFVGSLKAMSANMLADNGRTTVIRPLVYASEEEIVDFMGQVGLPVVSCNCPVSDSTDLKRRRMKELLKELEQ
EIPHIRSSMLKALSNVHPRHLLDQQLQGL

Sequences:

>Translated_269_residues
MLRTPKAASDPMTLTQQEMRELPLFRRLRHAVGKAVADFAMIREGDRIAVGVSGGKDSYTLLLLLEELRRRAPIDFQLVA
VIIDSGYPGYRGDIVRDYVTSLGIPCHLETTTHYEIITEKRRPGSSYCSICARLKRGALYGLADSLGCNKLALGHHGDDF
IETLLLNQFFVGSLKAMSANMLADNGRTTVIRPLVYASEEEIVDFMGQVGLPVVSCNCPVSDSTDLKRRRMKELLKELEQ
EIPHIRSSMLKALSNVHPRHLLDQQLQGL
>Mature_269_residues
MLRTPKAASDPMTLTQQEMRELPLFRRLRHAVGKAVADFAMIREGDRIAVGVSGGKDSYTLLLLLEELRRRAPIDFQLVA
VIIDSGYPGYRGDIVRDYVTSLGIPCHLETTTHYEIITEKRRPGSSYCSICARLKRGALYGLADSLGCNKLALGHHGDDF
IETLLLNQFFVGSLKAMSANMLADNGRTTVIRPLVYASEEEIVDFMGQVGLPVVSCNCPVSDSTDLKRRRMKELLKELEQ
EIPHIRSSMLKALSNVHPRHLLDQQLQGL

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32)

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family

Homologues:

Organism=Escherichia coli, GI1787606, Length=254, Percent_Identity=41.7322834645669, Blast_Score=211, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI193211090, Length=246, Percent_Identity=33.739837398374, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17540090, Length=253, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24651972, Length=234, Percent_Identity=29.0598290598291, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTCA_PELPD (A1AUS1)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_903149.1
- ProteinModelPortal:   A1AUS1
- STRING:   A1AUS1
- GeneID:   4574348
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_3499
- eggNOG:   COG0037
- HOGENOM:   HBG347038
- OMA:   EHILPEY
- PhylomeDB:   A1AUS1
- ProtClustDB:   PRK10696
- GO:   GO:0005737
- HAMAP:   MF_01850
- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF004976

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 30035; Mature: 30035

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRTPKAASDPMTLTQQEMRELPLFRRLRHAVGKAVADFAMIREGDRIAVGVSGGKDSYT
CCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHH
LLLLLEELRRRAPIDFQLVAVIIDSGYPGYRGDIVRDYVTSLGIPCHLETTTHYEIITEK
HHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHCCC
RRPGSSYCSICARLKRGALYGLADSLGCNKLALGHHGDDFIETLLLNQFFVGSLKAMSAN
CCCCHHHHHHHHHHHHCHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
MLADNGRTTVIRPLVYASEEEIVDFMGQVGLPVVSCNCPVSDSTDLKRRRMKELLKELEQ
HHHCCCCCEEEHHHHHCCHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHH
EIPHIRSSMLKALSNVHPRHLLDQQLQGL
HHHHHHHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure
MLRTPKAASDPMTLTQQEMRELPLFRRLRHAVGKAVADFAMIREGDRIAVGVSGGKDSYT
CCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHH
LLLLLEELRRRAPIDFQLVAVIIDSGYPGYRGDIVRDYVTSLGIPCHLETTTHYEIITEK
HHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHCCC
RRPGSSYCSICARLKRGALYGLADSLGCNKLALGHHGDDFIETLLLNQFFVGSLKAMSAN
CCCCHHHHHHHHHHHHCHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
MLADNGRTTVIRPLVYASEEEIVDFMGQVGLPVVSCNCPVSDSTDLKRRRMKELLKELEQ
HHHCCCCCEEEHHHHHCCHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHH
EIPHIRSSMLKALSNVHPRHLLDQQLQGL
HHHHHHHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA