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The map label for this gene is nifE [H]

Identifier: 118581891

GI number: 118581891

Start: 3854111

End: 3856870

Strand: Reverse

Name: nifE [H]

Synonym: Ppro_3491

Alternate gene names: 118581891

Gene position: 3856870-3854111 (Counterclockwise)

Preceding gene: 118581899

Following gene: 118581890

Centisome position: 96.23

GC content: 65.0

Gene sequence:

>2760_bases
ATGGCAAAACCTGACTACTACGATGTAACCGATTGCGACACCCACGACAAGGGCGCCCCCAAGTTCTGCAAGAAATCCGA
GCCGGGCGAGGGGACCGAGCGGAGCTGCGCCTACGACGGCGCCCGGGTGGTGCTGATGCCGATCACCGACGTGATCCACC
TGGTACACGGCCCCATCGCCTGCGCCGGCAACTCCTGGGACAACCGCGGGGCCCGCTCCTCCGGCTCCCAGCTCTACCGG
CGCGGCTTCACCACCGAGATGCTGGAGAACGACGTGGTCTTCGGCGGCGAGAAGAAGCTGTACAAGGCCATACTTGACCT
GGCCGAGCGCTACCGCGACCAGGCCAAGGCCATCTTCGTCTACGCCACCTGCGTCACCGCCATGACCGGCGACGACGTGG
AGGCGGTCTGCAAGGCTGCCCAGCCCAAGGCGGGTATGCCGGTCATACCGATCAACGCCCCCGGCTTCATCGGCGACAAG
AACATCGGCAACCGCCTGGCCGGGGAGATCATGTTCAAGCATGTCATCGGAACGGCAGAGCCGCCTGAACTGGGGGAGTA
CCCCATCAACCTGATCGGCGAGTACAACATTGCCGGCGACCTGTGGGGCATGCTGCCGCTGTTCCAGCGGTTGGGGATCC
AGATCCTCTCCTGCTTCAGCGGAGACGCGAAATTCGAGGAGTTGCGCTACGCCCACCGGGCCAAGCTGAACGTGATCATC
TGCTCCAAGAGCCTGACCAACCTGGCCAAGAAGATGCAGAAAACCTACGGTATGCCCTATCTGGAGGAATCATTCTACGG
CATGACCGACACGGCCAAGGCGCTGCGCGACATCGCCCGGGAGCTGGACAACACGGTCAACGGCCTGGAGAAGCGGGTCA
TGCAGGACCGGGTGGAGAAGCTCCTGGCAGAGGAAGAGGAAAAATGCCGCAAACGCATCGCCCCCTACCGGGCCAGGCTG
GAGGGGAAACGGGCGGTGCTGTTCACCGGCGGGGTCAAGACCTGGTCCATGGTCAACGCCCTGCGGGAGCTGGGGGTGGA
GATCCTGGCCGCCGGCACCCAGAACTCGACCCTGGAGGACTTCTACCGCATGAAGGCGCTGATGCACGCCGATGCCGGGA
TCATCGAGGATACCAGCACCGCGGGGCTTTTGGCCGTGATGCGGGAGAAGATGCCCGACCTGATCGTGGCCGGCGGCAAG
ACCAAGTTCCTGGCGCTGAAGACCAAGACCCCCTTCCTGGACATCAATCATGGCCGCAGCCACCCCTACGCCGGCTACGA
AGGGATGGTCACCTTTGCCAAACAGCTGGACCTGACGGTGAACAACCCGATCTGGCCGCTCCTGAACGCCCCGGCCCCCT
GGGAGAAGAGCCCCGACCAGCTGGCCGAAGACCTGGTGGAGGTGGCCGGCCATGGCGAGCGCTTCCTGGCCGAGGATCTC
TCCGCCTCAAGAGTCAGGGTCAGCACCAAGAGTGCGGTGGTCAACCCCCAGAAGAACTCGCCGGCCCTGGGGGCGACCCT
GGCCTACCTGGGGATCGACAACATGCTGGGGCTCCTCCACGGCGCCCAGGGGTGCTCCACCTTCATCAGGTTGCAGCTCT
CCCGCCACTTCAAGGAGTCCATCGCCCTGAATGCCACCGCCATGAGCGAGGAGTCGGCCATCTTCGGCGGCTGGGAGAAC
CTGAAGCAGGGGATCAAGCGGGTGATGGAGAAGTTCCACCCCGGTGTGGTGGGGGTGATGACCTCGGGCCTGACCGAAAC
CATGGGGGACGACGTGCAGAGCGCCATCGTCCACTTCCGCCGGGAAAACCCGGAACTGGCCGACACACCGGTCATCCACG
CCTCCACCCCCGATTACTGCGGCTCGCTGCAGGAGGGGTACGCCGCGGCGGTTGAGGCGATCCTGTCCACCCTGCCCGAG
GGGGGCACGGCCGTTCCCGGCCAGGTCAACCTGCTCCCCGGCAGCCACCTGACCCCGGCGGACGTGGAGCAGATCAGGGA
GCTGATGGAGGAATTCGGTCTGACGGTGCTGACCATCCCGGACATATCCTGCGCCATGGACGGCCACATCGACGAGCAGG
TATCGCCCCTCTCCACCGGCGGGATTGCGGTGGAGGCCATCCGGCGGGCCGGTCGCAGCGTTGCCACCATCTACGTGGGG
GATTCCCTGGCCAGGGCTGCCCTGAAAATGAAGGAGAAATTCGCAATCCCGGCCTATGGTTTTACCTCTCTCTCCGGGCT
GGGAGAGACCGACCTGTTCATGGAGACCATGAGCGCACTCTCCGGTCGCCCCATCCCGGAGAAGCAGCAGCGCTGGCGCA
GCCGCCTCATGGACGCCATGGTGGACAGTCACTACCAGTTCGGCGCCAAGAGGGTTGCCCTGGCCCTGGAGTCGGACAAC
CTGAAGAGCCTGACCACCTTCCTGGCCGGCATGGGCTGCCAGATCCAGGCAGCACTGAGCGCCACCCGCACCCGTGGCCT
GGACAGCCTTCCCTGCGACAACGTTTTTGTGGGGGACCTGGAGGATCTGGAGACCGCCGCCCAGGGGGTGGACCTGCTGG
TGGCCAACAGCAACGGCCGCCAGGCTGCGGCACGGCTGAAGATCGGCGCCCACCTGCGGGCAGGTCTGCCGGTGTTCGAC
CGCCTGGGCGCCCACCAGAAGATGTGGGTCGGCTACCGGGGAAGCATGAACCTGCTGTTCGAGGTGGCCAACCTGTTCCA
GGCCAATGCCAAGGAGGCGCAGAAGCTGGCGCATAATTGA

Upstream 100 bases:

>100_bases
AACAGTACCGAACGATCAATGGCAACGGCGCCAGGCACCTTCGATGCGGAAGAGTGCGGGCGCCTTTTTTTTTGAGCGGT
TGACGGGCAGGAGAACTCCC

Downstream 100 bases:

>100_bases
AGGTTTGAAAAGGAGCATCCCATGAAAATTGCATTTACGACGAAAACCGGCGACATGATCGACCTGCACTTCGGTCAGGC
AGATGCCTTCCACATCTGGG

Product: bifunctional nitrogenase molybdenum-cofactor biosynthesis protein NifE/NifN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 919; Mature: 918

Protein sequence:

>919_residues
MAKPDYYDVTDCDTHDKGAPKFCKKSEPGEGTERSCAYDGARVVLMPITDVIHLVHGPIACAGNSWDNRGARSSGSQLYR
RGFTTEMLENDVVFGGEKKLYKAILDLAERYRDQAKAIFVYATCVTAMTGDDVEAVCKAAQPKAGMPVIPINAPGFIGDK
NIGNRLAGEIMFKHVIGTAEPPELGEYPINLIGEYNIAGDLWGMLPLFQRLGIQILSCFSGDAKFEELRYAHRAKLNVII
CSKSLTNLAKKMQKTYGMPYLEESFYGMTDTAKALRDIARELDNTVNGLEKRVMQDRVEKLLAEEEEKCRKRIAPYRARL
EGKRAVLFTGGVKTWSMVNALRELGVEILAAGTQNSTLEDFYRMKALMHADAGIIEDTSTAGLLAVMREKMPDLIVAGGK
TKFLALKTKTPFLDINHGRSHPYAGYEGMVTFAKQLDLTVNNPIWPLLNAPAPWEKSPDQLAEDLVEVAGHGERFLAEDL
SASRVRVSTKSAVVNPQKNSPALGATLAYLGIDNMLGLLHGAQGCSTFIRLQLSRHFKESIALNATAMSEESAIFGGWEN
LKQGIKRVMEKFHPGVVGVMTSGLTETMGDDVQSAIVHFRRENPELADTPVIHASTPDYCGSLQEGYAAAVEAILSTLPE
GGTAVPGQVNLLPGSHLTPADVEQIRELMEEFGLTVLTIPDISCAMDGHIDEQVSPLSTGGIAVEAIRRAGRSVATIYVG
DSLARAALKMKEKFAIPAYGFTSLSGLGETDLFMETMSALSGRPIPEKQQRWRSRLMDAMVDSHYQFGAKRVALALESDN
LKSLTTFLAGMGCQIQAALSATRTRGLDSLPCDNVFVGDLEDLETAAQGVDLLVANSNGRQAAARLKIGAHLRAGLPVFD
RLGAHQKMWVGYRGSMNLLFEVANLFQANAKEAQKLAHN

Sequences:

>Translated_919_residues
MAKPDYYDVTDCDTHDKGAPKFCKKSEPGEGTERSCAYDGARVVLMPITDVIHLVHGPIACAGNSWDNRGARSSGSQLYR
RGFTTEMLENDVVFGGEKKLYKAILDLAERYRDQAKAIFVYATCVTAMTGDDVEAVCKAAQPKAGMPVIPINAPGFIGDK
NIGNRLAGEIMFKHVIGTAEPPELGEYPINLIGEYNIAGDLWGMLPLFQRLGIQILSCFSGDAKFEELRYAHRAKLNVII
CSKSLTNLAKKMQKTYGMPYLEESFYGMTDTAKALRDIARELDNTVNGLEKRVMQDRVEKLLAEEEEKCRKRIAPYRARL
EGKRAVLFTGGVKTWSMVNALRELGVEILAAGTQNSTLEDFYRMKALMHADAGIIEDTSTAGLLAVMREKMPDLIVAGGK
TKFLALKTKTPFLDINHGRSHPYAGYEGMVTFAKQLDLTVNNPIWPLLNAPAPWEKSPDQLAEDLVEVAGHGERFLAEDL
SASRVRVSTKSAVVNPQKNSPALGATLAYLGIDNMLGLLHGAQGCSTFIRLQLSRHFKESIALNATAMSEESAIFGGWEN
LKQGIKRVMEKFHPGVVGVMTSGLTETMGDDVQSAIVHFRRENPELADTPVIHASTPDYCGSLQEGYAAAVEAILSTLPE
GGTAVPGQVNLLPGSHLTPADVEQIRELMEEFGLTVLTIPDISCAMDGHIDEQVSPLSTGGIAVEAIRRAGRSVATIYVG
DSLARAALKMKEKFAIPAYGFTSLSGLGETDLFMETMSALSGRPIPEKQQRWRSRLMDAMVDSHYQFGAKRVALALESDN
LKSLTTFLAGMGCQIQAALSATRTRGLDSLPCDNVFVGDLEDLETAAQGVDLLVANSNGRQAAARLKIGAHLRAGLPVFD
RLGAHQKMWVGYRGSMNLLFEVANLFQANAKEAQKLAHN
>Mature_918_residues
AKPDYYDVTDCDTHDKGAPKFCKKSEPGEGTERSCAYDGARVVLMPITDVIHLVHGPIACAGNSWDNRGARSSGSQLYRR
GFTTEMLENDVVFGGEKKLYKAILDLAERYRDQAKAIFVYATCVTAMTGDDVEAVCKAAQPKAGMPVIPINAPGFIGDKN
IGNRLAGEIMFKHVIGTAEPPELGEYPINLIGEYNIAGDLWGMLPLFQRLGIQILSCFSGDAKFEELRYAHRAKLNVIIC
SKSLTNLAKKMQKTYGMPYLEESFYGMTDTAKALRDIARELDNTVNGLEKRVMQDRVEKLLAEEEEKCRKRIAPYRARLE
GKRAVLFTGGVKTWSMVNALRELGVEILAAGTQNSTLEDFYRMKALMHADAGIIEDTSTAGLLAVMREKMPDLIVAGGKT
KFLALKTKTPFLDINHGRSHPYAGYEGMVTFAKQLDLTVNNPIWPLLNAPAPWEKSPDQLAEDLVEVAGHGERFLAEDLS
ASRVRVSTKSAVVNPQKNSPALGATLAYLGIDNMLGLLHGAQGCSTFIRLQLSRHFKESIALNATAMSEESAIFGGWENL
KQGIKRVMEKFHPGVVGVMTSGLTETMGDDVQSAIVHFRRENPELADTPVIHASTPDYCGSLQEGYAAAVEAILSTLPEG
GTAVPGQVNLLPGSHLTPADVEQIRELMEEFGLTVLTIPDISCAMDGHIDEQVSPLSTGGIAVEAIRRAGRSVATIYVGD
SLARAALKMKEKFAIPAYGFTSLSGLGETDLFMETMSALSGRPIPEKQQRWRSRLMDAMVDSHYQFGAKRVALALESDNL
KSLTTFLAGMGCQIQAALSATRTRGLDSLPCDNVFVGDLEDLETAAQGVDLLVANSNGRQAAARLKIGAHLRAGLPVFDR
LGAHQKMWVGYRGSMNLLFEVANLFQANAKEAQKLAHN

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 100117; Mature: 99986

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPDYYDVTDCDTHDKGAPKFCKKSEPGEGTERSCAYDGARVVLMPITDVIHLVHGPIA
CCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHCCCEE
CAGNSWDNRGARSSGSQLYRRGFTTEMLENDVVFGGEKKLYKAILDLAERYRDQAKAIFV
ECCCCCCCCCCCCCHHHHHHHCCCHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHEEEE
YATCVTAMTGDDVEAVCKAAQPKAGMPVIPINAPGFIGDKNIGNRLAGEIMFKHVIGTAE
EEHHHHHCCCCCHHHHHHHCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
PPELGEYPINLIGEYNIAGDLWGMLPLFQRLGIQILSCFSGDAKFEELRYAHRAKLNVII
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCEEEEEE
CSKSLTNLAKKMQKTYGMPYLEESFYGMTDTAKALRDIARELDNTVNGLEKRVMQDRVEK
ECHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLAEEEEKCRKRIAPYRARLEGKRAVLFTGGVKTWSMVNALRELGVEILAAGTQNSTLED
HHHHHHHHHHHHHCHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCHHEEECCCCCCHHHH
FYRMKALMHADAGIIEDTSTAGLLAVMREKMPDLIVAGGKTKFLALKTKTPFLDINHGRS
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCEEEEEEECCCCEEECCCCCC
HPYAGYEGMVTFAKQLDLTVNNPIWPLLNAPAPWEKSPDQLAEDLVEVAGHGERFLAEDL
CCCCCCHHHHHHHHHHCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCC
SASRVRVSTKSAVVNPQKNSPALGATLAYLGIDNMLGLLHGAQGCSTFIRLQLSRHFKES
CCCCEEEEHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
IALNATAMSEESAIFGGWENLKQGIKRVMEKFHPGVVGVMTSGLTETMGDDVQSAIVHFR
HHCCHHHCCCCCHHCCCHHHHHHHHHHHHHHHCCCEEEHHHHCHHHHHHHHHHHHHHHHH
RENPELADTPVIHASTPDYCGSLQEGYAAAVEAILSTLPEGGTAVPGQVNLLPGSHLTPA
CCCCCCCCCCEEECCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHH
DVEQIRELMEEFGLTVLTIPDISCAMDGHIDEQVSPLSTGGIAVEAIRRAGRSVATIYVG
HHHHHHHHHHHHCCEEEEECCCHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEEC
DSLARAALKMKEKFAIPAYGFTSLSGLGETDLFMETMSALSGRPIPEKQQRWRSRLMDAM
HHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VDSHYQFGAKRVALALESDNLKSLTTFLAGMGCQIQAALSATRTRGLDSLPCDNVFVGDL
HHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCCEEHHHHHHHHHCCCCCCCCCCEEECCH
EDLETAAQGVDLLVANSNGRQAAARLKIGAHLRAGLPVFDRLGAHQKMWVGYRGSMNLLF
HHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEEECCCHHHHH
EVANLFQANAKEAQKLAHN
HHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure 
AKPDYYDVTDCDTHDKGAPKFCKKSEPGEGTERSCAYDGARVVLMPITDVIHLVHGPIA
CCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHCCCEE
CAGNSWDNRGARSSGSQLYRRGFTTEMLENDVVFGGEKKLYKAILDLAERYRDQAKAIFV
ECCCCCCCCCCCCCHHHHHHHCCCHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHEEEE
YATCVTAMTGDDVEAVCKAAQPKAGMPVIPINAPGFIGDKNIGNRLAGEIMFKHVIGTAE
EEHHHHHCCCCCHHHHHHHCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
PPELGEYPINLIGEYNIAGDLWGMLPLFQRLGIQILSCFSGDAKFEELRYAHRAKLNVII
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCEEEEEE
CSKSLTNLAKKMQKTYGMPYLEESFYGMTDTAKALRDIARELDNTVNGLEKRVMQDRVEK
ECHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLAEEEEKCRKRIAPYRARLEGKRAVLFTGGVKTWSMVNALRELGVEILAAGTQNSTLED
HHHHHHHHHHHHHCHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCHHEEECCCCCCHHHH
FYRMKALMHADAGIIEDTSTAGLLAVMREKMPDLIVAGGKTKFLALKTKTPFLDINHGRS
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCEEEEEEECCCCEEECCCCCC
HPYAGYEGMVTFAKQLDLTVNNPIWPLLNAPAPWEKSPDQLAEDLVEVAGHGERFLAEDL
CCCCCCHHHHHHHHHHCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCC
SASRVRVSTKSAVVNPQKNSPALGATLAYLGIDNMLGLLHGAQGCSTFIRLQLSRHFKES
CCCCEEEEHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
IALNATAMSEESAIFGGWENLKQGIKRVMEKFHPGVVGVMTSGLTETMGDDVQSAIVHFR
HHCCHHHCCCCCHHCCCHHHHHHHHHHHHHHHCCCEEEHHHHCHHHHHHHHHHHHHHHHH
RENPELADTPVIHASTPDYCGSLQEGYAAAVEAILSTLPEGGTAVPGQVNLLPGSHLTPA
CCCCCCCCCCEEECCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHH
DVEQIRELMEEFGLTVLTIPDISCAMDGHIDEQVSPLSTGGIAVEAIRRAGRSVATIYVG
HHHHHHHHHHHHCCEEEEECCCHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEEC
DSLARAALKMKEKFAIPAYGFTSLSGLGETDLFMETMSALSGRPIPEKQQRWRSRLMDAM
HHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VDSHYQFGAKRVALALESDNLKSLTTFLAGMGCQIQAALSATRTRGLDSLPCDNVFVGDL
HHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCCEEHHHHHHHHHCCCCCCCCCCEEECCH
EDLETAAQGVDLLVANSNGRQAAARLKIGAHLRAGLPVFDRLGAHQKMWVGYRGSMNLLF
HHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEEECCCHHHHH
EVANLFQANAKEAQKLAHN
HHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]