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The map label for this gene is nasD [H]

Identifier: 118581896

GI number: 118581896

Start: 3863066

End: 3864628

Strand: Direct

Name: nasD [H]

Synonym: Ppro_3496

Alternate gene names: 118581896

Gene position: 3863066-3864628 (Clockwise)

Preceding gene: 118581895

Following gene: 118581897

Centisome position: 96.38

GC content: 63.79

Gene sequence:

>1563_bases
ATGTTACGCAGTACTACTGTCCCGTCGAATATTGAGATTCTTACCCCAATAAAGGAGCACGCCATGACCAACGAAAAGCG
ATGGATCTGCACCCTCTGCGGCTATGTCCATCAGGGAAGCACGCCCCCCGACACATGCCCGATCTGCGGAGCCACCCCCG
ATCTGTTCGAACTTCAGACGACAACGGAAGCACCGCGCCAGGAAAGCGCTAGTGACCAGTGGGTCTGCCTGACCTGCGAC
TACATCCACCAGGGAACCTCCCCTCCGGCAACTTGCCCGATTTGCGGCCAGGGCGCGGAGCAGTTCGAACCGCACAGGGC
TGACCAGCAGGGCGCGGGCGCGGCAGAAGCTGTTTCCGTCGTCATCGTCGGCGCCGGCATCGCCGGGGTCTCCGCAGCCG
AGTCGATCCGTCGCGCATCTCCCGCGGCGCGAATCACCCTGCTGGGCAAGGAACCGGACCTCCCCTACTACCGCATCAAC
CTGACCCGCTACCTGGCCGGAGAAATCTCCCGCGACGACCTGACCATGCACCCGGAGCAGTGGTACACCGACAACGACAT
CATTCTCCGCCTCTCCACCGAGATGCGCGGCTTCGACCCCGCGAGCGGGCATCTGACCCTGCACACCCACGAGCAGCTCG
CCTTCGACCGCCTGATCCTGGCCATGGGGGCCCACCCCTTCGTCCCCCCCATCCCCGGAGCCACCCGCAAAAACGTGACG
GTCCTGCGCACCCTGGGGGACGCCGACTTCATCCTCGGCCAGCTGCGCCCCGGCCTGAACTGCACCATCATCGGCGGCGG
CGTCCTGGGGCTGGAGGCGGCCGGTGCCCTGGTCAAGCGCGGCGCATCTGTTACCCAGCTGGAAGGGTTCCCCTGGCTGA
TGCCCCAGCAACTCAACCGTACCGCCGGCGGGCTGCTGGCCAGACATGCCGAAAAACTAGGCATCACAATCCGCACCGAC
GCGCGCATCAAACAGATCGACGGCGACGAACGGGTGCACGGCGTCCTGCTGGAGAGCGGAGAGAGCATCGCTTCGGACCT
GGTGGTCATCACTGCCGGAGTACGCTGCAACAGCTATCTGCCGCGCCAGGCACAGCTTACGGTCAACCAGGGGGTAGTTG
TCGACGACCATCTCAAGAGCAGCGCAGGGAACATCCTTGCCGTGGGGGATCTTGCCGAGCACCGCGGCGTTTGCTACGGC
ACCTGGGTTCCGGCCCAGTTCCAGGGAAGCATCGCCGGGCTGAACGCCGTGGGAGGAAAGGTGGAGTTCGCCGGTATCCC
CCGCTCCAACACCCTCAAGGTGCTCAACTTCGACCTGTTCAGCATCGGACAGGTTCACCCCGACGACGGCAGCTACCAGA
GCTTTGAACAGATGGGCAACGATTGCTATCACTATGTCGTTTTCCGGGACAGCCGGCTGGTGGGCGCAATCCTGATGGGA
GACATGGGACCGGCAGCAGCCATCAAGCACCTGATCGAAGCCAGGACGTCCTGCAGCGATCTGCTGCACAGAAATCCCGA
CATAGATGGGATCCTTGCCTTCATCGCGGCCGGTTCCCGCTAG

Upstream 100 bases:

>100_bases
GCTTGGGTTTTCGTACCAAACCCCTTTAAGCCTCCCCTTGTCAGGGGAGGCTTAAAGGCTTCCCCCCTGACAAGGGGGGA
CTGAGGGGGGTTGGTTTTAG

Downstream 100 bases:

>100_bases
GTTTCCTGGCTGCCCCTCCGCCCACAATCCGTTCGGAGGGCGGCGATGCCATTTCGGGGCGGACGCGTCCGGCGTATCCG
CCCCGAACATCGTGGAGCCG

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MLRSTTVPSNIEILTPIKEHAMTNEKRWICTLCGYVHQGSTPPDTCPICGATPDLFELQTTTEAPRQESASDQWVCLTCD
YIHQGTSPPATCPICGQGAEQFEPHRADQQGAGAAEAVSVVIVGAGIAGVSAAESIRRASPAARITLLGKEPDLPYYRIN
LTRYLAGEISRDDLTMHPEQWYTDNDIILRLSTEMRGFDPASGHLTLHTHEQLAFDRLILAMGAHPFVPPIPGATRKNVT
VLRTLGDADFILGQLRPGLNCTIIGGGVLGLEAAGALVKRGASVTQLEGFPWLMPQQLNRTAGGLLARHAEKLGITIRTD
ARIKQIDGDERVHGVLLESGESIASDLVVITAGVRCNSYLPRQAQLTVNQGVVVDDHLKSSAGNILAVGDLAEHRGVCYG
TWVPAQFQGSIAGLNAVGGKVEFAGIPRSNTLKVLNFDLFSIGQVHPDDGSYQSFEQMGNDCYHYVVFRDSRLVGAILMG
DMGPAAAIKHLIEARTSCSDLLHRNPDIDGILAFIAAGSR

Sequences:

>Translated_520_residues
MLRSTTVPSNIEILTPIKEHAMTNEKRWICTLCGYVHQGSTPPDTCPICGATPDLFELQTTTEAPRQESASDQWVCLTCD
YIHQGTSPPATCPICGQGAEQFEPHRADQQGAGAAEAVSVVIVGAGIAGVSAAESIRRASPAARITLLGKEPDLPYYRIN
LTRYLAGEISRDDLTMHPEQWYTDNDIILRLSTEMRGFDPASGHLTLHTHEQLAFDRLILAMGAHPFVPPIPGATRKNVT
VLRTLGDADFILGQLRPGLNCTIIGGGVLGLEAAGALVKRGASVTQLEGFPWLMPQQLNRTAGGLLARHAEKLGITIRTD
ARIKQIDGDERVHGVLLESGESIASDLVVITAGVRCNSYLPRQAQLTVNQGVVVDDHLKSSAGNILAVGDLAEHRGVCYG
TWVPAQFQGSIAGLNAVGGKVEFAGIPRSNTLKVLNFDLFSIGQVHPDDGSYQSFEQMGNDCYHYVVFRDSRLVGAILMG
DMGPAAAIKHLIEARTSCSDLLHRNPDIDGILAFIAAGSR
>Mature_520_residues
MLRSTTVPSNIEILTPIKEHAMTNEKRWICTLCGYVHQGSTPPDTCPICGATPDLFELQTTTEAPRQESASDQWVCLTCD
YIHQGTSPPATCPICGQGAEQFEPHRADQQGAGAAEAVSVVIVGAGIAGVSAAESIRRASPAARITLLGKEPDLPYYRIN
LTRYLAGEISRDDLTMHPEQWYTDNDIILRLSTEMRGFDPASGHLTLHTHEQLAFDRLILAMGAHPFVPPIPGATRKNVT
VLRTLGDADFILGQLRPGLNCTIIGGGVLGLEAAGALVKRGASVTQLEGFPWLMPQQLNRTAGGLLARHAEKLGITIRTD
ARIKQIDGDERVHGVLLESGESIASDLVVITAGVRCNSYLPRQAQLTVNQGVVVDDHLKSSAGNILAVGDLAEHRGVCYG
TWVPAQFQGSIAGLNAVGGKVEFAGIPRSNTLKVLNFDLFSIGQVHPDDGSYQSFEQMGNDCYHYVVFRDSRLVGAILMG
DMGPAAAIKHLIEARTSCSDLLHRNPDIDGILAFIAAGSR

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=328, Percent_Identity=25.3048780487805, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI65787454, Length=328, Percent_Identity=25.3048780487805, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI226437568, Length=328, Percent_Identity=25.3048780487805, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1789765, Length=385, Percent_Identity=28.051948051948, Blast_Score=121, Evalue=9e-29,
Organism=Escherichia coli, GI1789065, Length=315, Percent_Identity=26.6666666666667, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788892, Length=281, Percent_Identity=29.8932384341637, Blast_Score=105, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17559934, Length=278, Percent_Identity=26.6187050359712, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24639257, Length=346, Percent_Identity=28.0346820809249, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI281359715, Length=346, Percent_Identity=28.0346820809249, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI281359713, Length=346, Percent_Identity=28.0346820809249, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24639250, Length=346, Percent_Identity=28.0346820809249, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI18543267, Length=346, Percent_Identity=28.0346820809249, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24639252, Length=346, Percent_Identity=28.0346820809249, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24585130, Length=318, Percent_Identity=27.0440251572327, Blast_Score=115, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 55864; Mature: 55864

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS50903 RUBREDOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRSTTVPSNIEILTPIKEHAMTNEKRWICTLCGYVHQGSTPPDTCPICGATPDLFELQT
CCCCCCCCCCCEEECCHHHHHCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCCCEEEEC
TTEAPRQESASDQWVCLTCDYIHQGTSPPATCPICGQGAEQFEPHRADQQGAGAAEAVSV
CCCCCCCCCCCCCEEEEEEHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHEEEE
VIVGAGIAGVSAAESIRRASPAARITLLGKEPDLPYYRINLTRYLAGEISRDDLTMHPEQ
EEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEEEEECCCCCCCCCEECCHH
WYTDNDIILRLSTEMRGFDPASGHLTLHTHEQLAFDRLILAMGAHPFVPPIPGATRKNVT
HCCCCCEEEEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
VLRTLGDADFILGQLRPGLNCTIIGGGVLGLEAAGALVKRGASVTQLEGFPWLMPQQLNR
EEEECCCHHHHHHHCCCCCEEEEEECCEEHHHHHHHHHHCCCCEEEECCCCCCCHHHHCH
TAGGLLARHAEKLGITIRTDARIKQIDGDERVHGVLLESGESIASDLVVITAGVRCNSYL
HHHHHHHHHHHHHCEEEECCCEEEECCCCCCEEEEEEECCCHHHCCEEEEEECCEECCCC
PRQAQLTVNQGVVVDDHLKSSAGNILAVGDLAEHRGVCYGTWVPAQFQGSIAGLNAVGGK
CCCEEEEECCCEEEEHHHHCCCCCEEEEECHHHHCCCEEEECCCHHHCCCEECCCCCCCE
VEFAGIPRSNTLKVLNFDLFSIGQVHPDDGSYQSFEQMGNDCYHYVVFRDSRLVGAILMG
EEEEECCCCCEEEEEEECEEECCEECCCCCCHHHHHHCCCCEEEEEEEECCCEEEEEEEC
DMGPAAAIKHLIEARTSCSDLLHRNPDIDGILAFIAAGSR
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MLRSTTVPSNIEILTPIKEHAMTNEKRWICTLCGYVHQGSTPPDTCPICGATPDLFELQT
CCCCCCCCCCCEEECCHHHHHCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCCCEEEEC
TTEAPRQESASDQWVCLTCDYIHQGTSPPATCPICGQGAEQFEPHRADQQGAGAAEAVSV
CCCCCCCCCCCCCEEEEEEHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHEEEE
VIVGAGIAGVSAAESIRRASPAARITLLGKEPDLPYYRINLTRYLAGEISRDDLTMHPEQ
EEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEEEEECCCCCCCCCEECCHH
WYTDNDIILRLSTEMRGFDPASGHLTLHTHEQLAFDRLILAMGAHPFVPPIPGATRKNVT
HCCCCCEEEEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
VLRTLGDADFILGQLRPGLNCTIIGGGVLGLEAAGALVKRGASVTQLEGFPWLMPQQLNR
EEEECCCHHHHHHHCCCCCEEEEEECCEEHHHHHHHHHHCCCCEEEECCCCCCCHHHHCH
TAGGLLARHAEKLGITIRTDARIKQIDGDERVHGVLLESGESIASDLVVITAGVRCNSYL
HHHHHHHHHHHHHCEEEECCCEEEECCCCCCEEEEEEECCCHHHCCEEEEEECCEECCCC
PRQAQLTVNQGVVVDDHLKSSAGNILAVGDLAEHRGVCYGTWVPAQFQGSIAGLNAVGGK
CCCEEEEECCCEEEEHHHHCCCCCEEEEECHHHHCCCEEEECCCHHHCCCEECCCCCCCE
VEFAGIPRSNTLKVLNFDLFSIGQVHPDDGSYQSFEQMGNDCYHYVVFRDSRLVGAILMG
EEEEECCCCCEEEEEEECEEECCEECCCCCCHHHHHHCCCCEEEEEEEECCCEEEEEEEC
DMGPAAAIKHLIEARTSCSDLLHRNPDIDGILAFIAAGSR
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]