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The map label for this gene is mutY [H]

Identifier: 118581886

GI number: 118581886

Start: 3851233

End: 3852132

Strand: Direct

Name: mutY [H]

Synonym: Ppro_3486

Alternate gene names: 118581886

Gene position: 3851233-3852132 (Clockwise)

Preceding gene: 118581885

Following gene: 118581892

Centisome position: 96.09

GC content: 63.56

Gene sequence:

>900_bases
ATGCTTTCACCCCAACGATTGACAGAACAGTACCATGCCGCGGGAGAGCTGGACGCGGCCACCGTGACAGCCTTCCGCCG
GGCGATCTATCGCCACTTCCACGCCAACCCGCGCCCCATGCCGTGGCGCGAGACCAGCGACCCGTACCATATCCTGGTAT
CGGAGGTCATGCTCCAGCAGACCCAGGTGGAGCGGGTCAAAGCCAAATACGCTCAGTTCCTGGAGACCTTCCCCACCGTC
AGGCACCTGGCAACCGCGCCACTGGAGGAGCTGCTGCGACTCTGGCAGGGGTTGGGGTACAACCGCCGCGCCATCGCCCT
CAAACGCTGCGCCGAACAGATCCATATCCTTCACGAGGGTAATTTCCCCACCACAATCCACGAGCTGCAGAACCTCCCCG
GCATCGGTCCCTACACCGCCCGAGCCGTTGCCGCTTTTGCCTTCGGTCTGCCTGAACCCTTCATCGAGACCAACATCCGC
ACCGTCTTCATCCACTTCTTCTTCCACAAACAAGAGGGGATCAGCGACCGCCAGCTCATGCCGCTGGTAGGGGCCACCCT
GGACCATTCCGATCCCCGCCACTGGTACTATGCCCTGATGGACTACGGCGTACGGCTCAAGCAGCTGTACCCCAACCCGG
GACGACGCAGCAGCCACCATGTGCGCCAATCCCCCTTCAAGGGCTCCAACCGTGAGCTGCGCAGCCGCATGCTGCGGGCG
GTCATGGAATGTCCCGGCATCCTGCCGGACGAGCTGGCAAAGGTGCTGGAGGCGGAAAGAGGTGCGGTGGAGAAGAACCT
GCAGGCGCTGGCGCGGGAGGGGTTCGTGGAACAGCGGGGGGACGGATTCGGGATCAGAACGTGCACATCACCCGGCGCAC
CCGATACTCCCCGCCGATGA

Upstream 100 bases:

>100_bases
AGGAGATGCTCGGGGCGGCCAAGCAGCTGGACTTCGAGAAGGCGGCCGAGCTGCGCGACCGGGTCAGGACGCTGGAGGAG
ATGGAGTTGGCCCTGCGCTG

Downstream 100 bases:

>100_bases
CCAGGTATTCCCCCTCCCCCTTCATGATGCTCCCCGGCAACAGATCGTTGTAGAAGAAGACCTTGCACAACGGCACCCCC
ACCTCCCACACCGTGGAACC

Product: HhH-GPD family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MLSPQRLTEQYHAAGELDAATVTAFRRAIYRHFHANPRPMPWRETSDPYHILVSEVMLQQTQVERVKAKYAQFLETFPTV
RHLATAPLEELLRLWQGLGYNRRAIALKRCAEQIHILHEGNFPTTIHELQNLPGIGPYTARAVAAFAFGLPEPFIETNIR
TVFIHFFFHKQEGISDRQLMPLVGATLDHSDPRHWYYALMDYGVRLKQLYPNPGRRSSHHVRQSPFKGSNRELRSRMLRA
VMECPGILPDELAKVLEAERGAVEKNLQALAREGFVEQRGDGFGIRTCTSPGAPDTPRR

Sequences:

>Translated_299_residues
MLSPQRLTEQYHAAGELDAATVTAFRRAIYRHFHANPRPMPWRETSDPYHILVSEVMLQQTQVERVKAKYAQFLETFPTV
RHLATAPLEELLRLWQGLGYNRRAIALKRCAEQIHILHEGNFPTTIHELQNLPGIGPYTARAVAAFAFGLPEPFIETNIR
TVFIHFFFHKQEGISDRQLMPLVGATLDHSDPRHWYYALMDYGVRLKQLYPNPGRRSSHHVRQSPFKGSNRELRSRMLRA
VMECPGILPDELAKVLEAERGAVEKNLQALAREGFVEQRGDGFGIRTCTSPGAPDTPRR
>Mature_299_residues
MLSPQRLTEQYHAAGELDAATVTAFRRAIYRHFHANPRPMPWRETSDPYHILVSEVMLQQTQVERVKAKYAQFLETFPTV
RHLATAPLEELLRLWQGLGYNRRAIALKRCAEQIHILHEGNFPTTIHELQNLPGIGPYTARAVAAFAFGLPEPFIETNIR
TVFIHFFFHKQEGISDRQLMPLVGATLDHSDPRHWYYALMDYGVRLKQLYPNPGRRSSHHVRQSPFKGSNRELRSRMLRA
VMECPGILPDELAKVLEAERGAVEKNLQALAREGFVEQRGDGFGIRTCTSPGAPDTPRR

Specific function: Adenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine:7,8-dihydro-8- oxoguanine (8-oxo-dGTP) [H]

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI115298650, Length=199, Percent_Identity=34.6733668341709, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI6912520, Length=199, Percent_Identity=34.6733668341709, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI115298654, Length=199, Percent_Identity=34.6733668341709, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI115298652, Length=199, Percent_Identity=34.6733668341709, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI190358497, Length=199, Percent_Identity=34.6733668341709, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI115298648, Length=198, Percent_Identity=34.8484848484849, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1789331, Length=130, Percent_Identity=43.0769230769231, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 34227; Mature: 34227

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSPQRLTEQYHAAGELDAATVTAFRRAIYRHFHANPRPMPWRETSDPYHILVSEVMLQQ
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
TQVERVKAKYAQFLETFPTVRHLATAPLEELLRLWQGLGYNRRAIALKRCAEQIHILHEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
NFPTTIHELQNLPGIGPYTARAVAAFAFGLPEPFIETNIRTVFIHFFFHKQEGISDRQLM
CCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHCHHHHHHHHHHHHHCCCCCCHHH
PLVGATLDHSDPRHWYYALMDYGVRLKQLYPNPGRRSSHHVRQSPFKGSNRELRSRMLRA
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHH
VMECPGILPDELAKVLEAERGAVEKNLQALAREGFVEQRGDGFGIRTCTSPGAPDTPRR
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCEEECCCCCCCCCCCC
>Mature Secondary Structure
MLSPQRLTEQYHAAGELDAATVTAFRRAIYRHFHANPRPMPWRETSDPYHILVSEVMLQQ
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
TQVERVKAKYAQFLETFPTVRHLATAPLEELLRLWQGLGYNRRAIALKRCAEQIHILHEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
NFPTTIHELQNLPGIGPYTARAVAAFAFGLPEPFIETNIRTVFIHFFFHKQEGISDRQLM
CCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHCHHHHHHHHHHHHHCCCCCCHHH
PLVGATLDHSDPRHWYYALMDYGVRLKQLYPNPGRRSSHHVRQSPFKGSNRELRSRMLRA
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHH
VMECPGILPDELAKVLEAERGAVEKNLQALAREGFVEQRGDGFGIRTCTSPGAPDTPRR
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2197596; 2001994; 9278503; 9846876 [H]