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The map label for this gene is thiE [H]

Identifier: 118581876

GI number: 118581876

Start: 3838909

End: 3839580

Strand: Direct

Name: thiE [H]

Synonym: Ppro_3476

Alternate gene names: 118581876

Gene position: 3838909-3839580 (Clockwise)

Preceding gene: 118581875

Following gene: 118581877

Centisome position: 95.78

GC content: 65.48

Gene sequence:

>672_bases
ATGTCGACCGTTTCCCGGGGTGAAGGACAATCCCGTTGCGTTGATTTTTCACTGTACCTGATCACCGACCGGCGTCAGAC
CGCCGGTCGGCCTCTGCTGGATGTCGTAGCCGCCGCCCTCAGGGGGGGCGTCGGGGCGGTGCAACTCAGGGAGAAGGACC
TGCCCGACAACGAACTATTAGAACTGGCCCGTGACCTGCGGCGCCTGACGCACGAACACCATGCCCGCCTGCTGATCAAC
CGCAGGGTGGATGTCTGTCAGGCGGTGGGCGCCGACGGCGTTCAGCTGGGAATCGAAGGGCTCTCCATAGTCGAGGCGCG
GCGGTTACTGGGCGATGGCCCGCTGATCGGCTATTCGGCCCATGCCGTGGAAGAGGCCCGCAGGGCAGAGGCTTCTGGCG
CGGACTTCGTCACCCTCAGCCCGGTATATCACACCCCTTCCAAGGCCCCCTTCGGCGAACCGCTGGGGCCGGACAGACTG
GGGGAGGCCTGCGGGGCCCTGGGCATTCCGGTATTCGCCCTGGGGGGCATCAAAGGAAGCACCATCGCCCGGGTCATGGC
AGCCGGAGCCCACGGCGTCGCCCTGATCTCGGCCATCACGGCAGCCGCCAACCCCGAAACCGAGGCAGCATCCTTACTAC
AGACGATCGAGCAGCATGAAACCCTTTCCTGA

Upstream 100 bases:

>100_bases
ATCTGGCGGTGAGGGCCGGCCGCCTCTCCTACAAGGCAGGCCGCATCCCGCGCAAGCTCTACGCCAACGCCTCCAGCCCG
CTGGATGGGATGATCGGCTG

Downstream 100 bases:

>100_bases
CCATCAGATCCACCCCGACCTCCGCATCAACTCCTATGGTCACTACCTGCGCCGCCGCTTCGGCTGTCGGGTCAGCAAGG
TCAACGTGGATGGCGGCTTC

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MSTVSRGEGQSRCVDFSLYLITDRRQTAGRPLLDVVAAALRGGVGAVQLREKDLPDNELLELARDLRRLTHEHHARLLIN
RRVDVCQAVGADGVQLGIEGLSIVEARRLLGDGPLIGYSAHAVEEARRAEASGADFVTLSPVYHTPSKAPFGEPLGPDRL
GEACGALGIPVFALGGIKGSTIARVMAAGAHGVALISAITAAANPETEAASLLQTIEQHETLS

Sequences:

>Translated_223_residues
MSTVSRGEGQSRCVDFSLYLITDRRQTAGRPLLDVVAAALRGGVGAVQLREKDLPDNELLELARDLRRLTHEHHARLLIN
RRVDVCQAVGADGVQLGIEGLSIVEARRLLGDGPLIGYSAHAVEEARRAEASGADFVTLSPVYHTPSKAPFGEPLGPDRL
GEACGALGIPVFALGGIKGSTIARVMAAGAHGVALISAITAAANPETEAASLLQTIEQHETLS
>Mature_222_residues
STVSRGEGQSRCVDFSLYLITDRRQTAGRPLLDVVAAALRGGVGAVQLREKDLPDNELLELARDLRRLTHEHHARLLINR
RVDVCQAVGADGVQLGIEGLSIVEARRLLGDGPLIGYSAHAVEEARRAEASGADFVTLSPVYHTPSKAPFGEPLGPDRLG
EACGALGIPVFALGGIKGSTIARVMAAGAHGVALISAITAAANPETEAASLLQTIEQHETLS

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=198, Percent_Identity=31.8181818181818, Blast_Score=62, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6325042, Length=224, Percent_Identity=32.5892857142857, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 23438; Mature: 23307

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVSRGEGQSRCVDFSLYLITDRRQTAGRPLLDVVAAALRGGVGAVQLREKDLPDNELL
CCCCCCCCCCCEEEEEEEEEEECCHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHH
ELARDLRRLTHEHHARLLINRRVDVCQAVGADGVQLGIEGLSIVEARRLLGDGPLIGYSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCEECCHH
HAVEEARRAEASGADFVTLSPVYHTPSKAPFGEPLGPDRLGEACGALGIPVFALGGIKGS
HHHHHHHHHHCCCCCEEEECCCEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCHH
TIARVMAAGAHGVALISAITAAANPETEAASLLQTIEQHETLS
HHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STVSRGEGQSRCVDFSLYLITDRRQTAGRPLLDVVAAALRGGVGAVQLREKDLPDNELL
CCCCCCCCCCEEEEEEEEEEECCHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHH
ELARDLRRLTHEHHARLLINRRVDVCQAVGADGVQLGIEGLSIVEARRLLGDGPLIGYSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCEECCHH
HAVEEARRAEASGADFVTLSPVYHTPSKAPFGEPLGPDRLGEACGALGIPVFALGGIKGS
HHHHHHHHHHCCCCCEEEECCCEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCHH
TIARVMAAGAHGVALISAITAAANPETEAASLLQTIEQHETLS
HHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA