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The map label for this gene is zraS [H]

Identifier: 118581752

GI number: 118581752

Start: 3700534

End: 3701976

Strand: Reverse

Name: zraS [H]

Synonym: Ppro_3352

Alternate gene names: 118581752

Gene position: 3701976-3700534 (Counterclockwise)

Preceding gene: 118581754

Following gene: 118581751

Centisome position: 92.36

GC content: 57.87

Gene sequence:

>1443_bases
ATGAAGCTGAACACCAAGCTCGTCATTATCATGCTCTCCCTGCTGGTCCTGACCATGATGACCCTCTTCACCCTCAACCA
GTACTCCCAGAACGACCTGGTCAACGAGATCCAGGAGAGTTCCCAGGAGATTTCCAAGGCTGTTCAGATGAGTATCGAGG
ATCTCACCTCCGATGCCGAGTTCTCACGCCTGAACGATTACCTGCACAAGGCAAGGAAGAAGGGGATCAACGAAATCAAC
ATCATCAACATCGAGGGTGAGATCGTCGACTCCTCCGACCCTGATAAAATCGGCAAGAGGCATGAACTGAACAAGCTGGA
AAAGGGGGTCCACGCAGCTCCTCGACATAACGGCGCCAGCATCCTCTCCCAGAAGCCATACGAGGTCGTGGTGCCGGTCA
TCGTGGGTGACGAGCACCTGGGATATGTGCAGATCAACATGTTGCTGGACAACATCAATAATCTGCAGCACGCCAACTTC
ATGCGCCGCCTATTCGCCACCGGTATGGTCTTCATGATCGGTATCGCCCTGACCATTTTTCTGGCGCGACGCTATACCGA
GCCGATCAACAGCCTGGTGGAAGACTTCAAGCGCGTCTCGGCGGGCGACCTGTCCGTTACCATTCCGGTGGACAGCAAGG
ATGAGATCGGCGAACTGGCCAAGGGTTTCAACAACATGGTGGAAAAGCTCCGCGAGCGTGAGGCGCTGGAGAAACGGCTC
TACGAAGCCGAGCATCTCTCGCGGGTTGGCCAGCTGGCATCGGGCATTGCCCACGAAATCCGCAATCCGCTCAACTACAT
CAGCCTGGCCATCGATCACCTTAAGGAAGAGATGCTCCCCCACTGCGCCGGAGCGCAGCGAGCGGAAATGGAAGAGCTGG
CCGATAAGATCAAGGAAGAAGTGCGCCGCGCAAACTACATGGTTGTCAACTTCATGAGCTATGGCCGCCCGCTCAAGCTG
CGACACGCATTGGTCCCCTACAGCGATGTGCTGGCCAAGGCGCTGCCGGTGCTGCATGACCGCCTGACGGAACAGCGCAT
AAGGCTTGAGCAGCGGATTCCAGACGATTTTCCGCCTCTGTGGGTCGACCAGGAGATGCTGCGCACCTGCATCCTGAACC
TGGTCGGAAACGCGGTCCAGGCCATGCCCGGTGGCGGTCTCATCACCTTGGGCGCCGAACGGGATCATGACCAGGTGCGG
CTGACCTTTTCCGACCAAGGCAAGGGTATACGCGCCGAAGACCTGGGCAAGATATTCCAGCCCTACTTCACCACCAGGGA
GGTGGGCATCGGCCTGGGGCTGGCGATCACCGAACGGATCGTCAGGGAACATGGCGGCTCCATACAGGTCGACAGCACGC
TGGGTAGCGGAACGACCTTTACGCTCCTGCTGCCGCTGGAACGGGGGCCACATGAGGGAAACCTTGAAACGCACGAACCA
TGA

Upstream 100 bases:

>100_bases
GTCAACAACCTGCGTGACGGATCGCGCTTGTTTCAGTCACTTTCCTACGTTATAGTGCGGAGATTGCCACTTATACCCCA
TTCGCTTTTTCAAGGCGGCC

Downstream 100 bases:

>100_bases
GACAATACGATACGGGGCTTGAAGTATGAACAACCAGCGGAAATCGGGAAGCATACTGATCGTTGACGACGAACGGGGGC
AGCGGGATATCCTCAAGCTG

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MKLNTKLVIIMLSLLVLTMMTLFTLNQYSQNDLVNEIQESSQEISKAVQMSIEDLTSDAEFSRLNDYLHKARKKGINEIN
IINIEGEIVDSSDPDKIGKRHELNKLEKGVHAAPRHNGASILSQKPYEVVVPVIVGDEHLGYVQINMLLDNINNLQHANF
MRRLFATGMVFMIGIALTIFLARRYTEPINSLVEDFKRVSAGDLSVTIPVDSKDEIGELAKGFNNMVEKLREREALEKRL
YEAEHLSRVGQLASGIAHEIRNPLNYISLAIDHLKEEMLPHCAGAQRAEMEELADKIKEEVRRANYMVVNFMSYGRPLKL
RHALVPYSDVLAKALPVLHDRLTEQRIRLEQRIPDDFPPLWVDQEMLRTCILNLVGNAVQAMPGGGLITLGAERDHDQVR
LTFSDQGKGIRAEDLGKIFQPYFTTREVGIGLGLAITERIVREHGGSIQVDSTLGSGTTFTLLLPLERGPHEGNLETHEP

Sequences:

>Translated_480_residues
MKLNTKLVIIMLSLLVLTMMTLFTLNQYSQNDLVNEIQESSQEISKAVQMSIEDLTSDAEFSRLNDYLHKARKKGINEIN
IINIEGEIVDSSDPDKIGKRHELNKLEKGVHAAPRHNGASILSQKPYEVVVPVIVGDEHLGYVQINMLLDNINNLQHANF
MRRLFATGMVFMIGIALTIFLARRYTEPINSLVEDFKRVSAGDLSVTIPVDSKDEIGELAKGFNNMVEKLREREALEKRL
YEAEHLSRVGQLASGIAHEIRNPLNYISLAIDHLKEEMLPHCAGAQRAEMEELADKIKEEVRRANYMVVNFMSYGRPLKL
RHALVPYSDVLAKALPVLHDRLTEQRIRLEQRIPDDFPPLWVDQEMLRTCILNLVGNAVQAMPGGGLITLGAERDHDQVR
LTFSDQGKGIRAEDLGKIFQPYFTTREVGIGLGLAITERIVREHGGSIQVDSTLGSGTTFTLLLPLERGPHEGNLETHEP
>Mature_480_residues
MKLNTKLVIIMLSLLVLTMMTLFTLNQYSQNDLVNEIQESSQEISKAVQMSIEDLTSDAEFSRLNDYLHKARKKGINEIN
IINIEGEIVDSSDPDKIGKRHELNKLEKGVHAAPRHNGASILSQKPYEVVVPVIVGDEHLGYVQINMLLDNINNLQHANF
MRRLFATGMVFMIGIALTIFLARRYTEPINSLVEDFKRVSAGDLSVTIPVDSKDEIGELAKGFNNMVEKLREREALEKRL
YEAEHLSRVGQLASGIAHEIRNPLNYISLAIDHLKEEMLPHCAGAQRAEMEELADKIKEEVRRANYMVVNFMSYGRPLKL
RHALVPYSDVLAKALPVLHDRLTEQRIRLEQRIPDDFPPLWVDQEMLRTCILNLVGNAVQAMPGGGLITLGAERDHDQVR
LTFSDQGKGIRAEDLGKIFQPYFTTREVGIGLGLAITERIVREHGGSIQVDSTLGSGTTFTLLLPLERGPHEGNLETHEP

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=402, Percent_Identity=29.3532338308458, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1788549, Length=238, Percent_Identity=30.672268907563, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1788393, Length=291, Percent_Identity=25.4295532646048, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789149, Length=382, Percent_Identity=27.2251308900524, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI87081816, Length=336, Percent_Identity=27.3809523809524, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786783, Length=340, Percent_Identity=25.5882352941176, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1790346, Length=261, Percent_Identity=27.9693486590038, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI48994928, Length=259, Percent_Identity=27.027027027027, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790300, Length=247, Percent_Identity=28.3400809716599, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790861, Length=435, Percent_Identity=22.9885057471264, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI145693157, Length=258, Percent_Identity=25.5813953488372, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789808, Length=328, Percent_Identity=21.6463414634146, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1786600, Length=221, Percent_Identity=25.3393665158371, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54005; Mature: 54005

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNTKLVIIMLSLLVLTMMTLFTLNQYSQNDLVNEIQESSQEISKAVQMSIEDLTSDAE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
FSRLNDYLHKARKKGINEINIINIEGEIVDSSDPDKIGKRHELNKLEKGVHAAPRHNGAS
HHHHHHHHHHHHHCCCCEEEEEEEECEEECCCCCHHHCHHHHHHHHHHCCCCCCCCCCHH
ILSQKPYEVVVPVIVGDEHLGYVQINMLLDNINNLQHANFMRRLFATGMVFMIGIALTIF
HHCCCCCEEEEEEEECCCCCCCEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LARRYTEPINSLVEDFKRVSAGDLSVTIPVDSKDEIGELAKGFNNMVEKLREREALEKRL
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEAEHLSRVGQLASGIAHEIRNPLNYISLAIDHLKEEMLPHCAGAQRAEMEELADKIKEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VRRANYMVVNFMSYGRPLKLRHALVPYSDVLAKALPVLHDRLTEQRIRLEQRIPDDFPPL
HHHCCCEEEEHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
WVDQEMLRTCILNLVGNAVQAMPGGGLITLGAERDHDQVRLTFSDQGKGIRAEDLGKIFQ
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHH
PYFTTREVGIGLGLAITERIVREHGGSIQVDSTLGSGTTFTLLLPLERGPHEGNLETHEP
HHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MKLNTKLVIIMLSLLVLTMMTLFTLNQYSQNDLVNEIQESSQEISKAVQMSIEDLTSDAE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
FSRLNDYLHKARKKGINEINIINIEGEIVDSSDPDKIGKRHELNKLEKGVHAAPRHNGAS
HHHHHHHHHHHHHCCCCEEEEEEEECEEECCCCCHHHCHHHHHHHHHHCCCCCCCCCCHH
ILSQKPYEVVVPVIVGDEHLGYVQINMLLDNINNLQHANFMRRLFATGMVFMIGIALTIF
HHCCCCCEEEEEEEECCCCCCCEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LARRYTEPINSLVEDFKRVSAGDLSVTIPVDSKDEIGELAKGFNNMVEKLREREALEKRL
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEAEHLSRVGQLASGIAHEIRNPLNYISLAIDHLKEEMLPHCAGAQRAEMEELADKIKEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VRRANYMVVNFMSYGRPLKLRHALVPYSDVLAKALPVLHDRLTEQRIRLEQRIPDDFPPL
HHHCCCEEEEHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
WVDQEMLRTCILNLVGNAVQAMPGGGLITLGAERDHDQVRLTFSDQGKGIRAEDLGKIFQ
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHH
PYFTTREVGIGLGLAITERIVREHGGSIQVDSTLGSGTTFTLLLPLERGPHEGNLETHEP
HHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]