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The map label for this gene is murD

Identifier: 118581691

GI number: 118581691

Start: 3627084

End: 3628424

Strand: Reverse

Name: murD

Synonym: Ppro_3291

Alternate gene names: 118581691

Gene position: 3628424-3627084 (Counterclockwise)

Preceding gene: 118581692

Following gene: 118581690

Centisome position: 90.53

GC content: 66.59

Gene sequence:

>1341_bases
ATGGAACTGAACGACAAGAAGGTGCTGGTGGTCGGCCTGGCCAAGACCGGTGTGGCCTGCGCCCGCTTCCTGGCTTCACG
GGGTGCGCGGGTGACGGTCACCGACATGCGCGACGAGGCCGCCCTGGCGGGGCAGCTGGCCCTGCTGGAGGGGCGCGGGA
TCAGGCGGGAGTTGACCCGCCACGACGCCGGGACCTTCACGCAGAGCCACCTGATCGTGGTCAGCCCGGGGGTTCCCCGC
ACCCTGCCGCAGCTGGTCATGGCGCACGAGGCAGGGGTGGAGATCATCAGCGAGATAGAGCTGGCTTCCCGCTTCATCCA
GGCGCCGCTGGTGGCCATCACCGGAACCAACGGCAAGACCACCGCCACCACCATCACCGGCGACATCTTCATCAAGAACG
GCTTTCGCACCTTCGTGGGGGGCAATATCGGCAATCCGCTGATCGAGCTGCTGGAGTCCGCGGAACCGGTGGAGAGGGTG
GTGGCAGAGATCAGCTCCTTCCAGCTGGAGTGGATCAGAGCTTTCCGCCCCGCTGTGGCCGCGCTTTTGAACCTGAGCGA
GGATCACCTGGACCGCTACGCCTCCTACCGGGAGTACATCGAGGCCAAGCTGCGCATCTTCGAGAACCAGACCGCGGATG
ACTACGCCGTGGTCAACGCCGACGACGAGCTGGTAATGGAGCATAGCCGTGGTCTCAGGGCGTGCCTGTTCCCCTTCAGC
CGGAAACAGGAGCTTGATGAGGGGATCTTCCACCGGGAAGGGCTCATCGTCTGGCGTCACAACGGTCGGGAGGAACGTTT
CCCCACCGCCGGCTTCCGCCTCCAGGGGGTGCATAACCTGGAGAACATCATGGCCGCCCTGGCATGTAGCCTGCTGCTGG
AGTGCCGGCCGACCGAGAGCCTGGCCTGCGTGCGCGAGTTCGAGGCCCTGCACCACCGCATGGAGTTCGTGCGCGAACTG
AACGGCGTGCGCTGGTACGAGGACAGCAAGGCCACCAACGTGGGCAGCGTGGAGAAGGCCCTGGAGAGTTTCGACGCCAT
AACCCTGATCGCCGGCGGCAAGGACAAGGGGGGCTCCTACTCCCCGCTCTCGCGGCTGGTGCGGGAGCGGGTGCGCCATC
TGGTGCTGATCGGCGAGGCGGCCGGCCGCATGCAGGAGGAGCTGGGGACGCTGACCGACACCCGCCGGGCCGCGACCCTG
GAGGAAGCGGTCAGTCTGGCAGCGGAGCTCACCGCTCCCGGCGGAACGGTACTCATGTCGCCGGCCTGCTCCAGTTTCGA
CATGTTCCGCGACTACGAGGAGCGGGCCCAGCGCTACATCGCGGCGGTCAAGGCGCTGTAG

Upstream 100 bases:

>100_bases
ATCGTCCGCTTCTGGATTATTACCATCATCCTGGCCCTGGTGGCGATCTCCACCCTGAAGATGCGCTAGCGCCGGGACGG
AAGAGAGGGTTTTTTTAAGC

Downstream 100 bases:

>100_bases
GGGCGACGCACCTGCCGTGAACCGCCTTGACTCGTTTCGGCGTTTTTTTGTGATAGCTCGTGACAGTTGTGTGACTCAAG
CTTCTTTCCGATTGTGCGTG

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MELNDKKVLVVGLAKTGVACARFLASRGARVTVTDMRDEAALAGQLALLEGRGIRRELTRHDAGTFTQSHLIVVSPGVPR
TLPQLVMAHEAGVEIISEIELASRFIQAPLVAITGTNGKTTATTITGDIFIKNGFRTFVGGNIGNPLIELLESAEPVERV
VAEISSFQLEWIRAFRPAVAALLNLSEDHLDRYASYREYIEAKLRIFENQTADDYAVVNADDELVMEHSRGLRACLFPFS
RKQELDEGIFHREGLIVWRHNGREERFPTAGFRLQGVHNLENIMAALACSLLLECRPTESLACVREFEALHHRMEFVREL
NGVRWYEDSKATNVGSVEKALESFDAITLIAGGKDKGGSYSPLSRLVRERVRHLVLIGEAAGRMQEELGTLTDTRRAATL
EEAVSLAAELTAPGGTVLMSPACSSFDMFRDYEERAQRYIAAVKAL

Sequences:

>Translated_446_residues
MELNDKKVLVVGLAKTGVACARFLASRGARVTVTDMRDEAALAGQLALLEGRGIRRELTRHDAGTFTQSHLIVVSPGVPR
TLPQLVMAHEAGVEIISEIELASRFIQAPLVAITGTNGKTTATTITGDIFIKNGFRTFVGGNIGNPLIELLESAEPVERV
VAEISSFQLEWIRAFRPAVAALLNLSEDHLDRYASYREYIEAKLRIFENQTADDYAVVNADDELVMEHSRGLRACLFPFS
RKQELDEGIFHREGLIVWRHNGREERFPTAGFRLQGVHNLENIMAALACSLLLECRPTESLACVREFEALHHRMEFVREL
NGVRWYEDSKATNVGSVEKALESFDAITLIAGGKDKGGSYSPLSRLVRERVRHLVLIGEAAGRMQEELGTLTDTRRAATL
EEAVSLAAELTAPGGTVLMSPACSSFDMFRDYEERAQRYIAAVKAL
>Mature_446_residues
MELNDKKVLVVGLAKTGVACARFLASRGARVTVTDMRDEAALAGQLALLEGRGIRRELTRHDAGTFTQSHLIVVSPGVPR
TLPQLVMAHEAGVEIISEIELASRFIQAPLVAITGTNGKTTATTITGDIFIKNGFRTFVGGNIGNPLIELLESAEPVERV
VAEISSFQLEWIRAFRPAVAALLNLSEDHLDRYASYREYIEAKLRIFENQTADDYAVVNADDELVMEHSRGLRACLFPFS
RKQELDEGIFHREGLIVWRHNGREERFPTAGFRLQGVHNLENIMAALACSLLLECRPTESLACVREFEALHHRMEFVREL
NGVRWYEDSKATNVGSVEKALESFDAITLIAGGKDKGGSYSPLSRLVRERVRHLVLIGEAAGRMQEELGTLTDTRRAATL
EEAVSLAAELTAPGGTVLMSPACSSFDMFRDYEERAQRYIAAVKAL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=448, Percent_Identity=37.7232142857143, Blast_Score=242, Evalue=3e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_PELPD (A1AU63)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_902941.1
- ProteinModelPortal:   A1AU63
- SMR:   A1AU63
- STRING:   A1AU63
- GeneID:   4572580
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_3291
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- PhylomeDB:   A1AU63
- ProtClustDB:   CLSK829090
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 49311; Mature: 49311

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNDKKVLVVGLAKTGVACARFLASRGARVTVTDMRDEAALAGQLALLEGRGIRRELTR
CCCCCCEEEEEEECHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHEECCCCHHHHHHH
HDAGTFTQSHLIVVSPGVPRTLPQLVMAHEAGVEIISEIELASRFIQAPLVAITGTNGKT
CCCCCEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCE
TATTITGDIFIKNGFRTFVGGNIGNPLIELLESAEPVERVVAEISSFQLEWIRAFRPAVA
EEEEEEEEEEEECCCHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ALLNLSEDHLDRYASYREYIEAKLRIFENQTADDYAVVNADDELVMEHSRGLRACLFPFS
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHCCCEEECCCCH
RKQELDEGIFHREGLIVWRHNGREERFPTAGFRLQGVHNLENIMAALACSLLLECRPTES
HHHHHHHHHHHHCCEEEEECCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCHH
LACVREFEALHHRMEFVRELNGVRWYEDSKATNVGSVEKALESFDAITLIAGGKDKGGSY
HHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCC
SPLSRLVRERVRHLVLIGEAAGRMQEELGTLTDTRRAATLEEAVSLAAELTAPGGTVLMS
CHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
PACSSFDMFRDYEERAQRYIAAVKAL
CCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELNDKKVLVVGLAKTGVACARFLASRGARVTVTDMRDEAALAGQLALLEGRGIRRELTR
CCCCCCEEEEEEECHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHEECCCCHHHHHHH
HDAGTFTQSHLIVVSPGVPRTLPQLVMAHEAGVEIISEIELASRFIQAPLVAITGTNGKT
CCCCCEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCE
TATTITGDIFIKNGFRTFVGGNIGNPLIELLESAEPVERVVAEISSFQLEWIRAFRPAVA
EEEEEEEEEEEECCCHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ALLNLSEDHLDRYASYREYIEAKLRIFENQTADDYAVVNADDELVMEHSRGLRACLFPFS
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHCCCEEECCCCH
RKQELDEGIFHREGLIVWRHNGREERFPTAGFRLQGVHNLENIMAALACSLLLECRPTES
HHHHHHHHHHHHCCEEEEECCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCHH
LACVREFEALHHRMEFVRELNGVRWYEDSKATNVGSVEKALESFDAITLIAGGKDKGGSY
HHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCC
SPLSRLVRERVRHLVLIGEAAGRMQEELGTLTDTRRAATLEEAVSLAAELTAPGGTVLMS
CHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
PACSSFDMFRDYEERAQRYIAAVKAL
CCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA