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The map label for this gene is hisH [H]

Identifier: 118581460

GI number: 118581460

Start: 3343273

End: 3343896

Strand: Reverse

Name: hisH [H]

Synonym: Ppro_3057

Alternate gene names: 118581460

Gene position: 3343896-3343273 (Counterclockwise)

Preceding gene: 118581461

Following gene: 118581459

Centisome position: 83.43

GC content: 57.53

Gene sequence:

>624_bases
GTGATTGCAATTATCGATTATGGCATGGGCAACCTCCGCTCCGTGCAGAAGGGCTTTGAAAGGGTCGGCGTCGAGGCGGT
TGTTACCGATGATCCCCGGCTGATCCTGGAGGCTGATAAGATCGTGCTCCCCGGCGTTGGGGCTTTCCGCGACTGCATGC
GCAACCTGGAGCAGGCCGGTTTTGTGGAGCCGATCCTCAAGGTGATCTCCGACGGTCGCCCTTTTCTGGGGATCTGCGTC
GGAATGCAGTTGCTCTTCAGCGATAGCTCGGAGTTCGGCCTGTATGCGGGGCTGAATGTAATACCCGGGCATGTGCTCCG
CTTTCCCGAAGGGATGCGGGAGGGGGGAGAACTGCTGAAGGTCCCGCACATGGGGTGGAACCAGCTCAATATCAGGCGTC
GGCCACCAGCCTTTGAGGGCATCGAAGAGGGCACCAACGTCTATTTCGTGCACTCGTACTACGTTAAGCCGGATGATGAC
GGCGTGGTTGCCACCACCACGGGCTACGGCTCTGAGTTCTGTTCAGCCGTTTGGAAGGACAACATCGTTGCCACCCAGTT
CCATCCGGAAAAGTCCCAGCATGCGGGCTTAAGCATACTGAAAAATTTCGCGGCACAAAATTGA

Upstream 100 bases:

>100_bases
TTACCCAGGACGTGATTACATTTCTGCAGAACTGGCTGGTCAACCATATCAAAGGTGTTGATAAGAAATACGGCCCCCAT
TTAACCAAGAACGGTGTAAC

Downstream 100 bases:

>100_bases
TACTGAAAGGTGCACCATGCTTGTAATTCCTGCGATTGACCTGAAAGACGGGGTTTGTGTTCGCCTTGAACAGGGGCTGA
TGGACAGGGACACGGTTTTC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MIAIIDYGMGNLRSVQKGFERVGVEAVVTDDPRLILEADKIVLPGVGAFRDCMRNLEQAGFVEPILKVISDGRPFLGICV
GMQLLFSDSSEFGLYAGLNVIPGHVLRFPEGMREGGELLKVPHMGWNQLNIRRRPPAFEGIEEGTNVYFVHSYYVKPDDD
GVVATTTGYGSEFCSAVWKDNIVATQFHPEKSQHAGLSILKNFAAQN

Sequences:

>Translated_207_residues
MIAIIDYGMGNLRSVQKGFERVGVEAVVTDDPRLILEADKIVLPGVGAFRDCMRNLEQAGFVEPILKVISDGRPFLGICV
GMQLLFSDSSEFGLYAGLNVIPGHVLRFPEGMREGGELLKVPHMGWNQLNIRRRPPAFEGIEEGTNVYFVHSYYVKPDDD
GVVATTTGYGSEFCSAVWKDNIVATQFHPEKSQHAGLSILKNFAAQN
>Mature_207_residues
MIAIIDYGMGNLRSVQKGFERVGVEAVVTDDPRLILEADKIVLPGVGAFRDCMRNLEQAGFVEPILKVISDGRPFLGICV
GMQLLFSDSSEFGLYAGLNVIPGHVLRFPEGMREGGELLKVPHMGWNQLNIRRRPPAFEGIEEGTNVYFVHSYYVKPDDD
GVVATTTGYGSEFCSAVWKDNIVATQFHPEKSQHAGLSILKNFAAQN

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=202, Percent_Identity=40.0990099009901, Blast_Score=127, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6319725, Length=217, Percent_Identity=36.8663594470046, Blast_Score=125, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22818; Mature: 22818

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAIIDYGMGNLRSVQKGFERVGVEAVVTDDPRLILEADKIVLPGVGAFRDCMRNLEQAG
CEEEEECCCCHHHHHHHHHHHCCCEEEEECCCEEEEEECCEEECCCHHHHHHHHHHHHCC
FVEPILKVISDGRPFLGICVGMQLLFSDSSEFGLYAGLNVIPGHVLRFPEGMREGGELLK
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHCCHHHCCCCCEEE
VPHMGWNQLNIRRRPPAFEGIEEGTNVYFVHSYYVKPDDDGVVATTTGYGSEFCSAVWKD
CCCCCCCEEEECCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEEECCCCHHHHHHHHHC
NIVATQFHPEKSQHAGLSILKNFAAQN
CEEEEEECCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIAIIDYGMGNLRSVQKGFERVGVEAVVTDDPRLILEADKIVLPGVGAFRDCMRNLEQAG
CEEEEECCCCHHHHHHHHHHHCCCEEEEECCCEEEEEECCEEECCCHHHHHHHHHHHHCC
FVEPILKVISDGRPFLGICVGMQLLFSDSSEFGLYAGLNVIPGHVLRFPEGMREGGELLK
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHCCHHHCCCCCEEE
VPHMGWNQLNIRRRPPAFEGIEEGTNVYFVHSYYVKPDDDGVVATTTGYGSEFCSAVWKD
CCCCCCCEEEECCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEEECCCCHHHHHHHHHC
NIVATQFHPEKSQHAGLSILKNFAAQN
CEEEEEECCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA