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The map label for this gene is hisF [H]

Identifier: 118581458

GI number: 118581458

Start: 3341754

End: 3342515

Strand: Reverse

Name: hisF [H]

Synonym: Ppro_3055

Alternate gene names: 118581458

Gene position: 3342515-3341754 (Counterclockwise)

Preceding gene: 118581459

Following gene: 118581457

Centisome position: 83.4

GC content: 59.45

Gene sequence:

>762_bases
ATGCTGACAAAGAGAATCATTCCCTGTCTGGATGTCAAGGGTGGCCGGGTCGTCAAGGGCGTGCAGTTTCTGGAATTGCG
TGATGCCGGTGATCCGGTGGAAATTGCGGAAATCTACGACCGGCAGGGCGCCGATGAGCTGACCTTTCTTGATATTACCG
CTTCCAGCGATGAGCGGGACATCATCATCGATGTGGTGCGCCGTACCGCCGAGCGGGTATTCATGCCGCTGACCGTTGGC
GGCGGCATCCGCACCGTGCAGGATATCCGTCGTCTCCTTAATGCAGGGGCGGACAAGACTTCCATCAATACCGCAGCGGT
GCATCGGCCGGAGTTCGTCCGCGAGGCGGCCGAACGCTTCGGCTCCCAGTGTACGGTTGTGGCCATCGATGCCCGCCGTG
TGCCAGGAGAGAAGCGCTGGGAGGTGTATACCCATGGAGGTCGCAATCCCACCGGGATCGATGCCGTGGAGTGGGCCGTC
AGGATGGAAGAGTTCGGCTCGGGCGAGATCCTGCTTACCAGCATGGATTGCGACGGCACCAAGGACGGCTATGATCTGGA
GCTGACCCGCGCAGTTGTAGATGCTGTCTCCATTCCGGTTATCGCATCGGGCGGAGTCGGAAATCTGGAACACCTCTATG
ACGGTTTTGCGCAGGGCGGCGCCAGTGCCTGTCTGGCCGCCTCCATTTTTCATTACCGCGAATATTCCATCGGAGAGGCC
AAACAGTATCTTAAGGAGAGAGGGGTGCCGGTCCGCCTATGA

Upstream 100 bases:

>100_bases
CGGGGTAACCGGGGTGATCACCGGCAAGGCGATCTACACCGGGGCTATCAGGCTGCATGAGGCCATCGCGCTCACCGGGA
AGGATAATCAGGAATAGGCT

Downstream 100 bases:

>100_bases
CAGAGCAGAATCACATTCTCGATGCCGTATACGGGGTTATTCTGGCGCGAAAGGCCACTCAGACGGAAAACTCTTACACC
GCCAGCCTGATGAGGAAGGG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MLTKRIIPCLDVKGGRVVKGVQFLELRDAGDPVEIAEIYDRQGADELTFLDITASSDERDIIIDVVRRTAERVFMPLTVG
GGIRTVQDIRRLLNAGADKTSINTAAVHRPEFVREAAERFGSQCTVVAIDARRVPGEKRWEVYTHGGRNPTGIDAVEWAV
RMEEFGSGEILLTSMDCDGTKDGYDLELTRAVVDAVSIPVIASGGVGNLEHLYDGFAQGGASACLAASIFHYREYSIGEA
KQYLKERGVPVRL

Sequences:

>Translated_253_residues
MLTKRIIPCLDVKGGRVVKGVQFLELRDAGDPVEIAEIYDRQGADELTFLDITASSDERDIIIDVVRRTAERVFMPLTVG
GGIRTVQDIRRLLNAGADKTSINTAAVHRPEFVREAAERFGSQCTVVAIDARRVPGEKRWEVYTHGGRNPTGIDAVEWAV
RMEEFGSGEILLTSMDCDGTKDGYDLELTRAVVDAVSIPVIASGGVGNLEHLYDGFAQGGASACLAASIFHYREYSIGEA
KQYLKERGVPVRL
>Mature_253_residues
MLTKRIIPCLDVKGGRVVKGVQFLELRDAGDPVEIAEIYDRQGADELTFLDITASSDERDIIIDVVRRTAERVFMPLTVG
GGIRTVQDIRRLLNAGADKTSINTAAVHRPEFVREAAERFGSQCTVVAIDARRVPGEKRWEVYTHGGRNPTGIDAVEWAV
RMEEFGSGEILLTSMDCDGTKDGYDLELTRAVVDAVSIPVIASGGVGNLEHLYDGFAQGGASACLAASIFHYREYSIGEA
KQYLKERGVPVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=43.1906614785992, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI87082028, Length=211, Percent_Identity=30.3317535545024, Blast_Score=84, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=36.3057324840764, Blast_Score=171, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27700; Mature: 27700

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKRIIPCLDVKGGRVVKGVQFLELRDAGDPVEIAEIYDRQGADELTFLDITASSDERD
CCCCCCCCEECCCCCEEECCEEEEEEECCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCH
IIIDVVRRTAERVFMPLTVGGGIRTVQDIRRLLNAGADKTSINTAAVHRPEFVREAAERF
HHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHH
GSQCTVVAIDARRVPGEKRWEVYTHGGRNPTGIDAVEWAVRMEEFGSGEILLTSMDCDGT
CCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCC
KDGYDLELTRAVVDAVSIPVIASGGVGNLEHLYDGFAQGGASACLAASIFHYREYSIGEA
CCCCCHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHH
KQYLKERGVPVRL
HHHHHHCCCCCCC
>Mature Secondary Structure
MLTKRIIPCLDVKGGRVVKGVQFLELRDAGDPVEIAEIYDRQGADELTFLDITASSDERD
CCCCCCCCEECCCCCEEECCEEEEEEECCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCH
IIIDVVRRTAERVFMPLTVGGGIRTVQDIRRLLNAGADKTSINTAAVHRPEFVREAAERF
HHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHH
GSQCTVVAIDARRVPGEKRWEVYTHGGRNPTGIDAVEWAVRMEEFGSGEILLTSMDCDGT
CCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCC
KDGYDLELTRAVVDAVSIPVIASGGVGNLEHLYDGFAQGGASACLAASIFHYREYSIGEA
CCCCCHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHH
KQYLKERGVPVRL
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA