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The map label for this gene is waaA [H]

Identifier: 118581432

GI number: 118581432

Start: 3308076

End: 3309383

Strand: Direct

Name: waaA [H]

Synonym: Ppro_3027

Alternate gene names: 118581432

Gene position: 3308076-3309383 (Clockwise)

Preceding gene: 118581431

Following gene: 118581433

Centisome position: 82.54

GC content: 65.14

Gene sequence:

>1308_bases
ATGTTCTACCTGACCTACAACATACTCTCCCTGTTCCTGCTCATCCCGGCCCTCTTCTACCACCTGTACCGCTCCGTCAA
CCGCGGCAGGCCGCCGGCCCTGGGGGAGCGCTTCGGCCGTATCCCGACTGAGGATCTGGCCAAGATCAACGGCCGCCCGG
TGATCTGGCTGCACGCAGTATCGGTGGGCGAGGCCATCGCGTCCCGGCCGCTACTCAAGGCGCTGCGCCAGCGCTATCCC
GGCCACGCCATAGTGATGTCCACCACCACCGAAACCGGCCGTTCCCTCGCTGCGGACTTCCCGGACAAGGATATCTGCAT
CTACTTCCCCTTCGACTTCCTGCCGGCGGTGAGGCGCACCCTGAATCGCATCAGACCGGACCTGATCATCATCATGGAGA
CCGAGATCTGGCCCAACTTCACCCGCGAGGCGCATCGGCGCGGCATTCCGCTGATCCTGGCCAATGGCCGCATATCGGAT
CGTTCCTTCACGGGGTACCTGCGATTCAGCTGGTTCTTCCGCCACCCGCTACGCCTCTTCTCCCGGCTCTGCATGCAGAG
CACAGCCGACGCCCGGCGGATCATCGCCATCGGCGCCCCCCCTGAGCGGGTACTCGTCGGCGGCAACCTGAAGTACGATA
TCCCCTTCCGCCAGATCCCCGGGAGCGAGCGACAGGCATTGCGGCACCGCTACGCCATTCCCCACGAGTTGACGGTAATC
ACCGCCGCCAGCACCCACGCCGGCGAGGAGGAACCGGTCATCGCCAGTTACCGAGAGCTGATGGCATCCCGCGACAACCT
GTTCCTGGTGCTCGTACCGCGCCACCCGGAGCGCTGCAGCGAGGTGGCCGCTGTTTTGGAGCGCAGTGGCATACCCTACT
GCCGCCGCACGGAACTGGACGCCAGGGGGAAGTTGTTCGTCAGAGGCGAGGTACTGCTGGTGGACAGCATCGGAGAGATG
ATGGGGCTGTATGCCCTGTCCGACCTGGCCTTCGTGGGGGGGAGCCTGATCCCCACCGGCGGGCACAACCTGCTGGAGCC
AGCCTCCCTCGGGGTAGCCAGCATTTTCGGCCCCCACATGACCAATTTCCGCGAGATCGCCGGTCTGGTGCTGGCCTGCG
GAGCCGGTATCCAGGTCGATTCGGCGGCCGGGCTGACCGCCGCCTGTGGCAGACTTTTGGATGACGCTGCCCTGCGCCGC
ACCATGGGAGCAAACGGCCTGGCCATGATGGAGGAAAACGGCGGCGCCACTGAGCGCCACATGGGGATGATCGGCGAACA
GTTCGTCGAACCGGTGGCGAAGGGATGA

Upstream 100 bases:

>100_bases
CGTGCCTGATGTTGCTCCACGTTCTCCGCGCCTCCGCGTGCTACTGCTTTTTCAAGGTTGAAACGAAAAAACTGATCTCT
CCAGAAACGCGGATGTCCGC

Downstream 100 bases:

>100_bases
ACTCCTCCCTTGCCACATACTGGCGCGCCCTGGCCAGCGGCACGCGCCGTTCCCTCCCCGACCGGTCGCTGCTGTCACTG
CTTGTCCCGTTCTCCCTGCT

Product: 3-deoxy-D-manno-octulosonic-acid transferase domain-containing protein

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MFYLTYNILSLFLLIPALFYHLYRSVNRGRPPALGERFGRIPTEDLAKINGRPVIWLHAVSVGEAIASRPLLKALRQRYP
GHAIVMSTTTETGRSLAADFPDKDICIYFPFDFLPAVRRTLNRIRPDLIIIMETEIWPNFTREAHRRGIPLILANGRISD
RSFTGYLRFSWFFRHPLRLFSRLCMQSTADARRIIAIGAPPERVLVGGNLKYDIPFRQIPGSERQALRHRYAIPHELTVI
TAASTHAGEEEPVIASYRELMASRDNLFLVLVPRHPERCSEVAAVLERSGIPYCRRTELDARGKLFVRGEVLLVDSIGEM
MGLYALSDLAFVGGSLIPTGGHNLLEPASLGVASIFGPHMTNFREIAGLVLACGAGIQVDSAAGLTAACGRLLDDAALRR
TMGANGLAMMEENGGATERHMGMIGEQFVEPVAKG

Sequences:

>Translated_435_residues
MFYLTYNILSLFLLIPALFYHLYRSVNRGRPPALGERFGRIPTEDLAKINGRPVIWLHAVSVGEAIASRPLLKALRQRYP
GHAIVMSTTTETGRSLAADFPDKDICIYFPFDFLPAVRRTLNRIRPDLIIIMETEIWPNFTREAHRRGIPLILANGRISD
RSFTGYLRFSWFFRHPLRLFSRLCMQSTADARRIIAIGAPPERVLVGGNLKYDIPFRQIPGSERQALRHRYAIPHELTVI
TAASTHAGEEEPVIASYRELMASRDNLFLVLVPRHPERCSEVAAVLERSGIPYCRRTELDARGKLFVRGEVLLVDSIGEM
MGLYALSDLAFVGGSLIPTGGHNLLEPASLGVASIFGPHMTNFREIAGLVLACGAGIQVDSAAGLTAACGRLLDDAALRR
TMGANGLAMMEENGGATERHMGMIGEQFVEPVAKG
>Mature_435_residues
MFYLTYNILSLFLLIPALFYHLYRSVNRGRPPALGERFGRIPTEDLAKINGRPVIWLHAVSVGEAIASRPLLKALRQRYP
GHAIVMSTTTETGRSLAADFPDKDICIYFPFDFLPAVRRTLNRIRPDLIIIMETEIWPNFTREAHRRGIPLILANGRISD
RSFTGYLRFSWFFRHPLRLFSRLCMQSTADARRIIAIGAPPERVLVGGNLKYDIPFRQIPGSERQALRHRYAIPHELTVI
TAASTHAGEEEPVIASYRELMASRDNLFLVLVPRHPERCSEVAAVLERSGIPYCRRTELDARGKLFVRGEVLLVDSIGEM
MGLYALSDLAFVGGSLIPTGGHNLLEPASLGVASIFGPHMTNFREIAGLVLACGAGIQVDSAAGLTAACGRLLDDAALRR
TMGANGLAMMEENGGATERHMGMIGEQFVEPVAKG

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=439, Percent_Identity=38.2687927107062, Blast_Score=267, Evalue=8e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 48205; Mature: 48205

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYLTYNILSLFLLIPALFYHLYRSVNRGRPPALGERFGRIPTEDLAKINGRPVIWLHAV
CEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHCCCCCEEEEEEC
SVGEAIASRPLLKALRQRYPGHAIVMSTTTETGRSLAADFPDKDICIYFPFDFLPAVRRT
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHCCCCCCCCEEEEECHHHHHHHHHH
LNRIRPDLIIIMETEIWPNFTREAHRRGIPLILANGRISDRSFTGYLRFSWFFRHPLRLF
HHHCCCCEEEEEECCCCCCHHHHHHHCCCCEEEECCEECCCCEEEEEEHHHHHHHHHHHH
SRLCMQSTADARRIIAIGAPPERVLVGGNLKYDIPFRQIPGSERQALRHRYAIPHELTVI
HHHHHHCCCCCCEEEEECCCCCEEEECCCEEECCCHHHCCCHHHHHHHHHHCCCCEEEEE
TAASTHAGEEEPVIASYRELMASRDNLFLVLVPRHPERCSEVAAVLERSGIPYCRRTELD
EEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEECCCCC
ARGKLFVRGEVLLVDSIGEMMGLYALSDLAFVGGSLIPTGGHNLLEPASLGVASIFGPHM
CCCEEEEEEEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHHCCCCC
TNFREIAGLVLACGAGIQVDSAAGLTAACGRLLDDAALRRTMGANGLAMMEENGGATERH
CCHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHH
MGMIGEQFVEPVAKG
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MFYLTYNILSLFLLIPALFYHLYRSVNRGRPPALGERFGRIPTEDLAKINGRPVIWLHAV
CEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHCCCCCEEEEEEC
SVGEAIASRPLLKALRQRYPGHAIVMSTTTETGRSLAADFPDKDICIYFPFDFLPAVRRT
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHCCCCCCCCEEEEECHHHHHHHHHH
LNRIRPDLIIIMETEIWPNFTREAHRRGIPLILANGRISDRSFTGYLRFSWFFRHPLRLF
HHHCCCCEEEEEECCCCCCHHHHHHHCCCCEEEECCEECCCCEEEEEEHHHHHHHHHHHH
SRLCMQSTADARRIIAIGAPPERVLVGGNLKYDIPFRQIPGSERQALRHRYAIPHELTVI
HHHHHHCCCCCCEEEEECCCCCEEEECCCEEECCCHHHCCCHHHHHHHHHHCCCCEEEEE
TAASTHAGEEEPVIASYRELMASRDNLFLVLVPRHPERCSEVAAVLERSGIPYCRRTELD
EEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEECCCCC
ARGKLFVRGEVLLVDSIGEMMGLYALSDLAFVGGSLIPTGGHNLLEPASLGVASIFGPHM
CCCEEEEEEEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHHCCCCC
TNFREIAGLVLACGAGIQVDSAAGLTAACGRLLDDAALRRTMGANGLAMMEENGGATERH
CCHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHH
MGMIGEQFVEPVAKG
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]