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The map label for this gene is icfA [H]

Identifier: 118581380

GI number: 118581380

Start: 3247069

End: 3247701

Strand: Reverse

Name: icfA [H]

Synonym: Ppro_2975

Alternate gene names: 118581380

Gene position: 3247701-3247069 (Counterclockwise)

Preceding gene: 118581381

Following gene: 118581379

Centisome position: 81.03

GC content: 62.88

Gene sequence:

>633_bases
ATGAAGGACATCGAGCGCTTTATTAGCGGGTTTCGCAGGTTCCGGGAAAACTACTTCGGACCAGAAGCCACCCATTTCGA
ACATCTCAAGAAGGGGCAGAACCCCAAAACCATGCTCATCGGCTGTGCCGACTCCCGGGTAGATCCGGCCATTCTGACCA
ATTGCGCCCCGGGCGACATCTTCACCGTGCGTAACGTAGCCAACCTGGTGCCCCCCTTCGAGGAGGACGGCGGCCGGCAC
GGGGTGAGCGCGGCACTGGAGTTCGCCGTCTGCCATCTGGAGGTGGAGCACATCATCGTCCTCGGTCACTCCGGGTGCGG
TGGCATCAATGCTCTCATGGCGGGTACCAGCGGCTGCGGGGGAGACGGTTTCATCTCCCGCTGGATGTCCATCGCGGCGC
CGGCCCGGGAACGGGTGCTGGCGGAGTTATCGGACAAGGACCCACTTCTTCAGCGGAGGGCTGCCGAGCAGGCGGCGATC
CTCCTCTCCCTGGAGAACCTCCGCTCCTTTCCCTGGATCGACGAGCGGGTCGCCAGCGGGGTGCTCTCGCTGCACGGCTG
GTATTTCGACATCTCGGAGGGGGAACTGCTGGAATATCGGCCCGAGCGTGGCGTATTCGAACCGATAACCTGA

Upstream 100 bases:

>100_bases
AGAAACACGCATTATTGTCTTTGACACCCAACGAGGCCCTTGCAGAATTAACGCCTTTACGGTAGCGGCACAGCTGCCAA
GGGACCGTCAAGGAGGATTC

Downstream 100 bases:

>100_bases
TACTTCAGATTCGTACGGTTTTTGTATTCACATACGAAACTCCGCTGTTCACCACCGACCTGCCCGACCTGAGGCTGGCC
GGACGTGACAAGGTCATCGG

Product: carbonate dehydratase

Products: NA

Alternate protein names: Carbonate dehydratase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKDIERFISGFRRFRENYFGPEATHFEHLKKGQNPKTMLIGCADSRVDPAILTNCAPGDIFTVRNVANLVPPFEEDGGRH
GVSAALEFAVCHLEVEHIIVLGHSGCGGINALMAGTSGCGGDGFISRWMSIAAPARERVLAELSDKDPLLQRRAAEQAAI
LLSLENLRSFPWIDERVASGVLSLHGWYFDISEGELLEYRPERGVFEPIT

Sequences:

>Translated_210_residues
MKDIERFISGFRRFRENYFGPEATHFEHLKKGQNPKTMLIGCADSRVDPAILTNCAPGDIFTVRNVANLVPPFEEDGGRH
GVSAALEFAVCHLEVEHIIVLGHSGCGGINALMAGTSGCGGDGFISRWMSIAAPARERVLAELSDKDPLLQRRAAEQAAI
LLSLENLRSFPWIDERVASGVLSLHGWYFDISEGELLEYRPERGVFEPIT
>Mature_210_residues
MKDIERFISGFRRFRENYFGPEATHFEHLKKGQNPKTMLIGCADSRVDPAILTNCAPGDIFTVRNVANLVPPFEEDGGRH
GVSAALEFAVCHLEVEHIIVLGHSGCGGINALMAGTSGCGGDGFISRWMSIAAPARERVLAELSDKDPLLQRRAAEQAAI
LLSLENLRSFPWIDERVASGVLSLHGWYFDISEGELLEYRPERGVFEPIT

Specific function: Reversible hydration of carbon dioxide. Essential to photosynthetic carbon dioxide fixation [H]

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm. Note=Associated with carboxysomes [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family [H]

Homologues:

Organism=Escherichia coli, GI1786534, Length=210, Percent_Identity=33.8095238095238, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1786318, Length=202, Percent_Identity=31.6831683168317, Blast_Score=86, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25147564, Length=237, Percent_Identity=28.6919831223629, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115532990, Length=230, Percent_Identity=30, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324292, Length=89, Percent_Identity=41.5730337078652, Blast_Score=69, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24645213, Length=238, Percent_Identity=26.890756302521, Blast_Score=84, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001765
- InterPro:   IPR015892 [H]

Pfam domain/function: PF00484 Pro_CA [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 23153; Mature: 23153

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00704 PROK_CO2_ANHYDRASE_1 ; PS00705 PROK_CO2_ANHYDRASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDIERFISGFRRFRENYFGPEATHFEHLKKGQNPKTMLIGCADSRVDPAILTNCAPGDI
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHCCCCCCCE
FTVRNVANLVPPFEEDGGRHGVSAALEFAVCHLEVEHIIVLGHSGCGGINALMAGTSGCG
EHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHEEEEEEEEEEECCCCCHHHHHHHCCCCCC
GDGFISRWMSIAAPARERVLAELSDKDPLLQRRAAEQAAILLSLENLRSFPWIDERVASG
CHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
VLSLHGWYFDISEGELLEYRPERGVFEPIT
HHHHCCEEEECCCCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MKDIERFISGFRRFRENYFGPEATHFEHLKKGQNPKTMLIGCADSRVDPAILTNCAPGDI
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHCCCCCCCE
FTVRNVANLVPPFEEDGGRHGVSAALEFAVCHLEVEHIIVLGHSGCGGINALMAGTSGCG
EHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHEEEEEEEEEEECCCCCHHHHHHHCCCCCC
GDGFISRWMSIAAPARERVLAELSDKDPLLQRRAAEQAAILLSLENLRSFPWIDERVASG
CHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
VLSLHGWYFDISEGELLEYRPERGVFEPIT
HHHHCCEEEECCCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]