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The map label for this gene is miaA

Identifier: 118581368

GI number: 118581368

Start: 3234505

End: 3235434

Strand: Direct

Name: miaA

Synonym: Ppro_2963

Alternate gene names: 118581368

Gene position: 3234505-3235434 (Clockwise)

Preceding gene: 118581367

Following gene: 118581369

Centisome position: 80.7

GC content: 64.62

Gene sequence:

>930_bases
ATGACAGGGCCGGCACTCCCCCGCATACTGATCATCTGCGGACCCACCGCCTCGGGCAAGAGCGAACTGGCGGTACGACT
GGCGCGGGAACTGGACGGAGAGATCGTCAATGCCGACTCCATGCAGATCCATCGCGGCATGGACATCGGCACGGCCAAGC
CGACCGTCGGGGAGATGGGCGCCGTCCCCCACCATCTGCTGGACGTGGCCGATCCGGACCGGCCTTTCTCGGCCGCCGAC
TTCAGCGACGCGGCCAGCCAGGCGATTTCAGGCATCATCCGGCGGGGAAAGCGCCCCATCGTGGTGGGGGGCACCGGCCT
CTACCTGCGCGCCCTGCTGCACGGCCTGGTGGACTCCCCCAGCGGCGCCGGAGAGCTGCGCCGGCGACTGCAGGAAGAGG
CGCGGGAACTGGGCAACCAGGCCATGCTGGAGCGGTTGCGCCGGGTTGACCCGCAACTTGCAACCACGATCCACCCCAAC
AATCTGGTGCGCATCATCCGCGGGCTGGAGGTGTACCACCTGACCGGCATCCCGCTCTCCCGCTACCAGCACGAGCACGG
CTTTGCCGCAGAGCGCTACCGGAGCCTCGCCATCGGCATCCGGGTGGAACGCCGGGAGCTGTACGAACGGATAGAACGGC
GGGTTGACCGGATGCTGGCCACGGGGCTCCTGGACGAGGTCCGGGCTCTGCTGGAGGCCGGTTTCGGACCGGAACTGAAG
GCCATGCGCTCCATAGGCTACCGTGAGTCCTGCGACTTCCTGGCGGGCAATTCAAGCCTGGAGGAGACAACAGCGCTGAT
AAAGCGCAACACCCGCCGCTACGCCAAGCGTCAACTGACCTGGTTCAATGCCGACCCGGAGATTATATGGCTTGAATATC
CGGAAAAGTTTGCTACTATTTTGCGACATTGCATTGCATTTTTCGAATGA

Upstream 100 bases:

>100_bases
ACGGCATGGACGGCACCGACTTCGCCGCCAGCTGCCCCCATGGCCGCCCGGTTTCCCATGTCATCACCCTGGGAGAACTG
GAGAGGATCTTCAAACGCAC

Downstream 100 bases:

>100_bases
CAGGAGGGATGACACCCATGGCAAAGGCCCCGTTCAACATTCAGGATCAGTACCTCAACCAGTCCCGGAAGGAACGCGTC
AAGGTGATCGTCACCATGAT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase 1; DMAPP:tRNA dimethylallyltransferase 1; DMATase 1; Isopentenyl-diphosphate:tRNA isopentenyltransferase 1; IPP transferase 1; IPPT 1; IPTase 1

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MTGPALPRILIICGPTASGKSELAVRLARELDGEIVNADSMQIHRGMDIGTAKPTVGEMGAVPHHLLDVADPDRPFSAAD
FSDAASQAISGIIRRGKRPIVVGGTGLYLRALLHGLVDSPSGAGELRRRLQEEARELGNQAMLERLRRVDPQLATTIHPN
NLVRIIRGLEVYHLTGIPLSRYQHEHGFAAERYRSLAIGIRVERRELYERIERRVDRMLATGLLDEVRALLEAGFGPELK
AMRSIGYRESCDFLAGNSSLEETTALIKRNTRRYAKRQLTWFNADPEIIWLEYPEKFATILRHCIAFFE

Sequences:

>Translated_309_residues
MTGPALPRILIICGPTASGKSELAVRLARELDGEIVNADSMQIHRGMDIGTAKPTVGEMGAVPHHLLDVADPDRPFSAAD
FSDAASQAISGIIRRGKRPIVVGGTGLYLRALLHGLVDSPSGAGELRRRLQEEARELGNQAMLERLRRVDPQLATTIHPN
NLVRIIRGLEVYHLTGIPLSRYQHEHGFAAERYRSLAIGIRVERRELYERIERRVDRMLATGLLDEVRALLEAGFGPELK
AMRSIGYRESCDFLAGNSSLEETTALIKRNTRRYAKRQLTWFNADPEIIWLEYPEKFATILRHCIAFFE
>Mature_308_residues
TGPALPRILIICGPTASGKSELAVRLARELDGEIVNADSMQIHRGMDIGTAKPTVGEMGAVPHHLLDVADPDRPFSAADF
SDAASQAISGIIRRGKRPIVVGGTGLYLRALLHGLVDSPSGAGELRRRLQEEARELGNQAMLERLRRVDPQLATTIHPNN
LVRIIRGLEVYHLTGIPLSRYQHEHGFAAERYRSLAIGIRVERRELYERIERRVDRMLATGLLDEVRALLEAGFGPELKA
MRSIGYRESCDFLAGNSSLEETTALIKRNTRRYAKRQLTWFNADPEIIWLEYPEKFATILRHCIAFFE

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=309, Percent_Identity=33.3333333333333, Blast_Score=145, Evalue=4e-35,
Organism=Escherichia coli, GI1790613, Length=300, Percent_Identity=40.6666666666667, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI25144712, Length=300, Percent_Identity=27, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324848, Length=286, Percent_Identity=33.5664335664336, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24649830, Length=328, Percent_Identity=32.9268292682927, Blast_Score=151, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA1_PELPD (A1AT90)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_902618.1
- ProteinModelPortal:   A1AT90
- SMR:   A1AT90
- STRING:   A1AT90
- GeneID:   4574469
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_2963
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   A1AT90
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34596; Mature: 34465

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGPALPRILIICGPTASGKSELAVRLARELDGEIVNADSMQIHRGMDIGTAKPTVGEMG
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEECCCCHHHHCCCCCCCCCCCCHHHC
AVPHHLLDVADPDRPFSAADFSDAASQAISGIIRRGKRPIVVGGTGLYLRALLHGLVDSP
CCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCC
SGAGELRRRLQEEARELGNQAMLERLRRVDPQLATTIHPNNLVRIIRGLEVYHLTGIPLS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEECCHHHHHHHHCCCEEEECCCCHH
RYQHEHGFAAERYRSLAIGIRVERRELYERIERRVDRMLATGLLDEVRALLEAGFGPELK
HHHHHCCHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AMRSIGYRESCDFLAGNSSLEETTALIKRNTRRYAKRQLTWFNADPEIIWLEYPEKFATI
HHHHCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHCEEECCCCCEEEEECCHHHHHH
LRHCIAFFE
HHHHHHHCC
>Mature Secondary Structure 
TGPALPRILIICGPTASGKSELAVRLARELDGEIVNADSMQIHRGMDIGTAKPTVGEMG
CCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEECCCCHHHHCCCCCCCCCCCCHHHC
AVPHHLLDVADPDRPFSAADFSDAASQAISGIIRRGKRPIVVGGTGLYLRALLHGLVDSP
CCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCC
SGAGELRRRLQEEARELGNQAMLERLRRVDPQLATTIHPNNLVRIIRGLEVYHLTGIPLS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEECCHHHHHHHHCCCEEEECCCCHH
RYQHEHGFAAERYRSLAIGIRVERRELYERIERRVDRMLATGLLDEVRALLEAGFGPELK
HHHHHCCHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AMRSIGYRESCDFLAGNSSLEETTALIKRNTRRYAKRQLTWFNADPEIIWLEYPEKFATI
HHHHCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHCEEECCCCCEEEEECCHHHHHH
LRHCIAFFE
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA