Click here to switch to the map view.

The map label for this gene is rsmE [H]

Identifier: 118581365

GI number: 118581365

Start: 3231168

End: 3231914

Strand: Direct

Name: rsmE [H]

Synonym: Ppro_2960

Alternate gene names: 118581365

Gene position: 3231168-3231914 (Clockwise)

Preceding gene: 118581364

Following gene: 118581366

Centisome position: 80.62

GC content: 64.39

Gene sequence:

>747_bases
ATGAGCGGCCGGCGCTTCATGATCAGCTCCCGCAGCATCCAGGACAACCATGCCTGGCTGGAGGGGGACCTGTTCAGCCA
CATGGTGCGGGTACTGCGGCTGGGTCCGGGCGCATCGGTCACCCTGGTGGACGAGCAGGGGGCTGAACACCGGGGATCCA
TCCAACAGGTGGAGAAAGAGAGGGTCATGGTCAGGATCGCGGGATCAGTTGTTCCCGGCAACGGCGAAGGGGAAACGCCG
GCCATAACCATCTGCCAGGCCCTTCCCAAGGGGGAGAAGACCGATCTGATCCTGCAGAAGGGAACCGAGCTGGGAGTGCA
TGACTTCCGCCTGTTCGCCGGGTGCCGTTCCGTTGCCCGCGTACGCGAGGAACAACACAAGGGAAAGCTGGAGCGCTGGA
ACAGGATCACCGCCGAGGCGGCGCGCCAGTGCGGCAGACGACGCATACCGGCGGTCAGCTGGTTTCCCGACGCGGAGCGG
GCGGCAGGCTCCTGCGACCATGAGCTGCGCCTGCTGCTCTGGGAGGGGGAACGCCGGCAAGGGCTGAAGAACGCCCTGGA
GCAGCTTCCCCGTCCCGCATCGGCCATTGTAGCCATCGGCCCCGAGGGAGGCTTCGATCCCCGAGAGGTTCGCTGCTTCA
TCGGGCACGGCTTTCTCCCCGTCAGCCTGGGGAAGAGGGTCCTGCGCACGGAAACGGCATCCCTTGCCATTACCGCAATA
CTTCAGTATATTTGGGGAAACATGTAG

Upstream 100 bases:

>100_bases
AGCTGGTCCGCGCCGGATTCGCCTGCCAAGAGCTGGAATACCGTGAGACGCGGCGGCAGGGTGAATGGGTGGCCATGGTC
TACCGGAGAGCCGCACGAGC

Downstream 100 bases:

>100_bases
CCCAGCATCCGCCGGCCGCTCCCAGCGGAGCCCCTGCCCGGCGGAGACAGGCTGCACAGAGGAGAATCCCATGAAATGTC
CGAAATGCGGGTATACCAGT

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MSGRRFMISSRSIQDNHAWLEGDLFSHMVRVLRLGPGASVTLVDEQGAEHRGSIQQVEKERVMVRIAGSVVPGNGEGETP
AITICQALPKGEKTDLILQKGTELGVHDFRLFAGCRSVARVREEQHKGKLERWNRITAEAARQCGRRRIPAVSWFPDAER
AAGSCDHELRLLLWEGERRQGLKNALEQLPRPASAIVAIGPEGGFDPREVRCFIGHGFLPVSLGKRVLRTETASLAITAI
LQYIWGNM

Sequences:

>Translated_248_residues
MSGRRFMISSRSIQDNHAWLEGDLFSHMVRVLRLGPGASVTLVDEQGAEHRGSIQQVEKERVMVRIAGSVVPGNGEGETP
AITICQALPKGEKTDLILQKGTELGVHDFRLFAGCRSVARVREEQHKGKLERWNRITAEAARQCGRRRIPAVSWFPDAER
AAGSCDHELRLLLWEGERRQGLKNALEQLPRPASAIVAIGPEGGFDPREVRCFIGHGFLPVSLGKRVLRTETASLAITAI
LQYIWGNM
>Mature_247_residues
SGRRFMISSRSIQDNHAWLEGDLFSHMVRVLRLGPGASVTLVDEQGAEHRGSIQQVEKERVMVRIAGSVVPGNGEGETPA
ITICQALPKGEKTDLILQKGTELGVHDFRLFAGCRSVARVREEQHKGKLERWNRITAEAARQCGRRRIPAVSWFPDAERA
AGSCDHELRLLLWEGERRQGLKNALEQLPRPASAIVAIGPEGGFDPREVRCFIGHGFLPVSLGKRVLRTETASLAITAIL
QYIWGNM

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=243, Percent_Identity=30.8641975308642, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27506; Mature: 27375

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGRRFMISSRSIQDNHAWLEGDLFSHMVRVLRLGPGASVTLVDEQGAEHRGSIQQVEKE
CCCCEEEEECCCCCCCCCEECHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHH
RVMVRIAGSVVPGNGEGETPAITICQALPKGEKTDLILQKGTELGVHDFRLFAGCRSVAR
HHEEEEECEEECCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHH
VREEQHKGKLERWNRITAEAARQCGRRRIPAVSWFPDAERAAGSCDHELRLLLWEGERRQ
HHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCEEEEEEECCHHHH
GLKNALEQLPRPASAIVAIGPEGGFDPREVRCFIGHGFLPVSLGKRVLRTETASLAITAI
HHHHHHHHCCCCCCEEEEECCCCCCCHHHEEEEECCCEEEHHHHHHHHHHHHHHHHHHHH
LQYIWGNM
HHHHHCCC
>Mature Secondary Structure 
SGRRFMISSRSIQDNHAWLEGDLFSHMVRVLRLGPGASVTLVDEQGAEHRGSIQQVEKE
CCCEEEEECCCCCCCCCEECHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHH
RVMVRIAGSVVPGNGEGETPAITICQALPKGEKTDLILQKGTELGVHDFRLFAGCRSVAR
HHEEEEECEEECCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHH
VREEQHKGKLERWNRITAEAARQCGRRRIPAVSWFPDAERAAGSCDHELRLLLWEGERRQ
HHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCEEEEEEECCHHHH
GLKNALEQLPRPASAIVAIGPEGGFDPREVRCFIGHGFLPVSLGKRVLRTETASLAITAI
HHHHHHHHCCCCCCEEEEECCCCCCCHHHEEEEECCCEEEHHHHHHHHHHHHHHHHHHHH
LQYIWGNM
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]