Click here to switch to the map view.

The map label for this gene is proB

Identifier: 118581358

GI number: 118581358

Start: 3223613

End: 3224734

Strand: Reverse

Name: proB

Synonym: Ppro_2953

Alternate gene names: 118581358

Gene position: 3224734-3223613 (Counterclockwise)

Preceding gene: 118581359

Following gene: 118581346

Centisome position: 80.46

GC content: 66.13

Gene sequence:

>1122_bases
ATGCGCCGGGAGCTTTTCAGGAAGATTAAGCGGGTGGTGGTCAAGATCGGCAGCAGGGTGCTGACAGACGATGAGGGCGC
CTTGGACATGGGGGTCATCGGACGCATCTGTGGAGATATCGCCAGCCTGCGCAGGCAGGGGCGCCAGGTGGTGCTGGTCT
CCTCCGGAGCCATCGCCGCCGGCCGCAGCGAGCTGGGCATGACCGAAAAGCCCCGGACCATACCGCACAAGCAGGCTGCG
GCGGCCATCGGCCAGACCCGCATGATGCGGGCCTACGAGGAGTCTTTTGCTCCCCACGGCCTCAAGGTGGCCCAGGTGCT
GCTTACCAGGGAGGACCTGGCCAGCCGGCAGCGTTTCCTGAATGCCCGTGCCACCCTGGACGCCCTCTTGGGCTTCGGCG
TCATCCCGGTGATCAACGAGAACGACACGGTGGTGGTGGAAGAGATCAAGTTTGGCGACAACGACAACCTCTCCGCCCTG
GTGACCAACGTGGCCGAGGCCGGGCTGCTGGTGATCATGACCGATATCGAGGGGTTTTACAGTGCTGATCCGCGCAGCAA
CCCGGATGCGGTGCTGGTGCCGCTGGTCCAGGGGATCACCCGCGAGATCGAGCGTGCCGCCGGCGGTTCCGGCTCCAGTG
TCGGCACCGGGGGCATGGCCACCAAGGTGGCGGCCGCCAAGAAGGCCGCCAAGAATGGTGTGCCCACCATCATCGTGCCG
GGCAAGCGCGAGGGGATCATCGCCACGCTCATGGCCGGCCAGGAGGTGGGGACCCTGTTTCTGCCGCTAGACGCCTGCCT
GAACCGGCGCAAGCACTGGCTCGCCTATTCCCTGAAACCGGCCGGGCGGATCATCGTGGATGACGGCGCCCGCGAGGTGC
TGCTGAAGAAGGGGAAGAGTCTGTTGCCCTCCGGCGTGCTGCGGGTGGAGGGGCGTTTCGAGCGGGGCGCCTGCGTTAGG
GTCTGCGGTTCGGACGAGCAGGAGTTCGCCCGGGGGCTGTCCGACTACTCCAGCAGCGAAATCGCCCGTCTGGCCGGCCA
GAGGAGCAGCCGCATCGAGGCCATCCTGGGCTACCGCTACGGTGACGTGATCATCCACCGGGACAATCTGGTGGTGCTGT
AA

Upstream 100 bases:

>100_bases
TCAGGGTCTTTCCGATCTCCTCCGCCACCGGCGAGGGGGTTGGCGAACTTTTGGACGAGATCGCCCGCCGCCTGTGGGGC
CGGATTGAAGAGGAGTGGTG

Downstream 100 bases:

>100_bases
GAAGATTCGTGAATTTTAACGAACAGGGCCCGCTTTTGCGGGCCCTGTTCGTTTCAAGGCTGTTCCGTTAATGCCCGGAA
CGCTGGCTGGTCGGCGCGGA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MRRELFRKIKRVVVKIGSRVLTDDEGALDMGVIGRICGDIASLRRQGRQVVLVSSGAIAAGRSELGMTEKPRTIPHKQAA
AAIGQTRMMRAYEESFAPHGLKVAQVLLTREDLASRQRFLNARATLDALLGFGVIPVINENDTVVVEEIKFGDNDNLSAL
VTNVAEAGLLVIMTDIEGFYSADPRSNPDAVLVPLVQGITREIERAAGGSGSSVGTGGMATKVAAAKKAAKNGVPTIIVP
GKREGIIATLMAGQEVGTLFLPLDACLNRRKHWLAYSLKPAGRIIVDDGAREVLLKKGKSLLPSGVLRVEGRFERGACVR
VCGSDEQEFARGLSDYSSSEIARLAGQRSSRIEAILGYRYGDVIIHRDNLVVL

Sequences:

>Translated_373_residues
MRRELFRKIKRVVVKIGSRVLTDDEGALDMGVIGRICGDIASLRRQGRQVVLVSSGAIAAGRSELGMTEKPRTIPHKQAA
AAIGQTRMMRAYEESFAPHGLKVAQVLLTREDLASRQRFLNARATLDALLGFGVIPVINENDTVVVEEIKFGDNDNLSAL
VTNVAEAGLLVIMTDIEGFYSADPRSNPDAVLVPLVQGITREIERAAGGSGSSVGTGGMATKVAAAKKAAKNGVPTIIVP
GKREGIIATLMAGQEVGTLFLPLDACLNRRKHWLAYSLKPAGRIIVDDGAREVLLKKGKSLLPSGVLRVEGRFERGACVR
VCGSDEQEFARGLSDYSSSEIARLAGQRSSRIEAILGYRYGDVIIHRDNLVVL
>Mature_373_residues
MRRELFRKIKRVVVKIGSRVLTDDEGALDMGVIGRICGDIASLRRQGRQVVLVSSGAIAAGRSELGMTEKPRTIPHKQAA
AAIGQTRMMRAYEESFAPHGLKVAQVLLTREDLASRQRFLNARATLDALLGFGVIPVINENDTVVVEEIKFGDNDNLSAL
VTNVAEAGLLVIMTDIEGFYSADPRSNPDAVLVPLVQGITREIERAAGGSGSSVGTGGMATKVAAAKKAAKNGVPTIIVP
GKREGIIATLMAGQEVGTLFLPLDACLNRRKHWLAYSLKPAGRIIVDDGAREVLLKKGKSLLPSGVLRVEGRFERGACVR
VCGSDEQEFARGLSDYSSSEIARLAGQRSSRIEAILGYRYGDVIIHRDNLVVL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=294, Percent_Identity=31.9727891156463, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI62912457, Length=292, Percent_Identity=31.8493150684932, Blast_Score=132, Evalue=5e-31,
Organism=Escherichia coli, GI1786437, Length=363, Percent_Identity=47.1074380165289, Blast_Score=324, Evalue=5e-90,
Organism=Caenorhabditis elegans, GI17569967, Length=274, Percent_Identity=37.2262773722628, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17569969, Length=274, Percent_Identity=37.2262773722628, Blast_Score=160, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6320506, Length=412, Percent_Identity=34.2233009708738, Blast_Score=233, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6321822, Length=417, Percent_Identity=32.8537170263789, Blast_Score=219, Evalue=4e-58,
Organism=Drosophila melanogaster, GI21357643, Length=296, Percent_Identity=33.1081081081081, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_PELPD (A1AT80)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_902608.1
- ProteinModelPortal:   A1AT80
- STRING:   A1AT80
- GeneID:   4574493
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_2953
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   A1AT80
- ProtClustDB:   PRK05429
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40095; Mature: 40095

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRELFRKIKRVVVKIGSRVLTDDEGALDMGVIGRICGDIASLRRQGRQVVLVSSGAIAA
CCHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GRSELGMTEKPRTIPHKQAAAAIGQTRMMRAYEESFAPHGLKVAQVLLTREDLASRQRFL
CCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
NARATLDALLGFGVIPVINENDTVVVEEIKFGDNDNLSALVTNVAEAGLLVIMTDIEGFY
HHHHHHHHHHHCCCEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCCC
SADPRSNPDAVLVPLVQGITREIERAAGGSGSSVGTGGMATKVAAAKKAAKNGVPTIIVP
CCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEC
GKREGIIATLMAGQEVGTLFLPLDACLNRRKHWLAYSLKPAGRIIVDDGAREVLLKKGKS
CCCCCEEEEEECCCCCCEEEEEHHHHHCCCHHEEEEEECCCCEEEEECCHHHHHHHCCCC
LLPSGVLRVEGRFERGACVRVCGSDEQEFARGLSDYSSSEIARLAGQRSSRIEAILGYRY
CCCCCEEEECCCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCCC
GDVIIHRDNLVVL
CCEEEECCCEEEC
>Mature Secondary Structure
MRRELFRKIKRVVVKIGSRVLTDDEGALDMGVIGRICGDIASLRRQGRQVVLVSSGAIAA
CCHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GRSELGMTEKPRTIPHKQAAAAIGQTRMMRAYEESFAPHGLKVAQVLLTREDLASRQRFL
CCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
NARATLDALLGFGVIPVINENDTVVVEEIKFGDNDNLSALVTNVAEAGLLVIMTDIEGFY
HHHHHHHHHHHCCCEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCCC
SADPRSNPDAVLVPLVQGITREIERAAGGSGSSVGTGGMATKVAAAKKAAKNGVPTIIVP
CCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEC
GKREGIIATLMAGQEVGTLFLPLDACLNRRKHWLAYSLKPAGRIIVDDGAREVLLKKGKS
CCCCCEEEEEECCCCCCEEEEEHHHHHCCCHHEEEEEECCCCEEEEECCHHHHHHHCCCC
LLPSGVLRVEGRFERGACVRVCGSDEQEFARGLSDYSSSEIARLAGQRSSRIEAILGYRY
CCCCCEEEECCCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCCC
GDVIIHRDNLVVL
CCEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA